• Title/Summary/Keyword: bacterial culture

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Quantitative analysis of oral disease-causing bacteria in saliva among bacterial culture, SYBRgreen qPCR and MRT-PCR method (타액내 구강질환 원인 균의 세균배양법, SYBR green qPCR법, MRT-PCR법 간의 정량분석)

  • Park, Yong-Duk;Oh, Hye-Young;Park, Bok-Ri;Cho, Ara;Kim, Dong-Kie;Jang, Jong-Hwa
    • Journal of Korean society of Dental Hygiene
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    • v.17 no.2
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    • pp.319-330
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    • 2017
  • Objectives: The purpose of this study was to compare SYBR Green qPCR, TaqMan, and bacterial selective medium cultures for accurate quantitative analysis of oral microorganisms. Methods: The SYBR Green method is widely used to analyze the total amount of oral microorganisms in oral saliva. However, in this study, MTR-PCR method based on TaqMan method was performed using newly developed primers and probes. In addition, it was designed to confirm the detection agreement of bacteria among bacteria detection method. Results: As a result of MRT-PCR and SYBR Green qPCR analysis, more than 40 times (0.9-362.9 times) bacterium was detected by MRT-PCR. In addition, more bacteria were detected in saliva in the order of MRT-PCR, SYBR Green qPCR, and bacterium culture, and the results of MRB-PCR and SYBR Green qPCR showed the highest agreement. The agreement between the three methods for detecting P. intermedia was similar between 71.4 and 88.6%, but the agreement between MRT-PCR and SYBR Green qPCR was 80% for S. mutans. Among them, the number of total bacteria, P. intermedia and S. mutans bacteria in saliva was higher than that of SYBR Green qPCR method, and bacterium culture method by MRT-PCR method. P. intermedia and S. mutans in saliva were detected by MRT-PCR and MRT-PCR in 88.6% of cases, followed by the SYBR Green qPCR method (80.0%). Conclusions: The SYBR Green qPCR method is the same molecular biology method, but it can not analyze the germs at the same time. Bacterial culturing takes a lot of time if there is no selective culture medium. Therefore, the MRT-PCR method using newly developed primers and probes is considered to be the best method.

혐기성 PCE 탈염소화 미생물 농화 배양 및 미생물 군집 해석

  • 문부영;이태호;박태주
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2004.04a
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    • pp.332-336
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    • 2004
  • An anaerobic PCE(tetrachloroethylene) dechlorinating bacterial culture from a landfill soil was enriched and characterized. The enrichment culture could dechlorinate 60$\mu$mol/$m\ell$ of PCE during a month of incubation and cis-DCE(cis-dichloroethylene) was observed as a main product of PCE dechlorination. Microbial analysis of the dechlorinating enrichment culture by rising PCR-DGGE (Polymerase chain reaction-Denaturing gradient gel electrophoresis) method showed that at least three microorganisms were related to the anaerobic PCE dechlorination.

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Characteristics of Culture Conditions for the Production of Crude Biosurfactant by Bacillus subtilis JK-1 (Bacillus subtilis JK-1의 생물계면활성제 생산을 위한 배양 특성)

  • Kim, Ji-Yeon
    • Journal of Applied Biological Chemistry
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    • v.54 no.3
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    • pp.153-158
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    • 2011
  • Optimal culture conditions were characterized for production of crude biosurfactant of Bacillus subtilis JK-1. During incubation of B. subtilis JK-1, the bacterial growth pattern, changes of the surface tension at variable temperatures, pH and NaCl concentrations in bacterial culture medium were studied. The strain was able to grow and produce biosurfactant at $15-45^{\circ}C$, in the pH range of 6-10, and at 0-10% (w/v) NaCl. In case, culture broth pH was gradually changed to neutral or weak alkaline. Optimal culture conditions for crude biosurfactant production were at $35^{\circ}C$ and pH 7.0 after 48 h incubation and the surface tension of biosurfactant was 24.0 mN/m. Besides, as the concentration of NaCl was increased from 0 to 10% (w/v), the growth was decreased, pH of the culture broth was converted from weak alkaline to acidic, and the surface tension rised.

Effect of a Microbial Product on the Control of Soilborne Diseases of Turfgrasses (미생물제에 의한 잔디의 토양전염병 방제 효과)

  • 박규진;김영호;박은경;김동성
    • Plant Disease and Agriculture
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    • v.1 no.1
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    • pp.19-29
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    • 1995
  • A microbial product composed of three antagonistic fungal isolates (Aspergillus sp., Penicillium sp. and Trichoderma sp.) and three bacterial isolates (Arthrobacter sp., Bacillus sp., and Pseudomonas sp.) was tested for the control of Pythium blight caused by Pythium sp., brown patch by Rhizoctonia solani (anastomosis group(AG) 1-1) and large patch by R. solani (AG 2-2) of turfgrasses. Cultures of the antagonistic fungi and bacteria varied in the effectiveness in reducing disease severity of Pytium blight and brown patch on bentgrass. The antagonistic fungal and bacterial isolates were mixed and cultured at 20-$25^{\circ}C$ for 3 days in a growth medium, and the diluted solution of the microbial culture was applied under the field conditions after inoculation of the above turfgrass pathogens. The treated turfgrass was incubated at 28$^{\circ}C$ in a growth chamber. In this experiment, Pythium blight was almost completely controlled and brown patch was slightly decreased by the microbial product, while no control was observed in large patch of zoysiagrass. In zoysiagrass treated with the microbial culture, thatch accumulation was notably reduced.

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Degradation of Rice Straw by Rumen Fungi and Cellulolytic Bacteria through Mono-, Co- or Sequential- Cultures

  • Ha, J.K.;Lee, S.S.;Kim, S.W.;Han, In K.;Ushida, K.;Cheng, K.J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.6
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    • pp.797-802
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    • 2001
  • Two strains of rumen fungi (Piromyces rhizinflata B157, Orpinomyces joyonii SG4) and three strains of rumen cellulolytic bacteria (Ruminococcus albus B199, Ruminococcus flavefaciens FD1 and Fibrobacter succinogenes S85) were used as mono-cultures or combinationally arranged as co- and sequential-cultures to assess the relative contributions and interactions between rumen fungi and cellulolytic bacteria on rice straw degradation. The rates of dry matter degradation of co-cultures were similar to those of corresponding bacterial mono-cultures. Compared to corresponding sequential-cultures, the degradation of rice straw was reduced in all co-cultures (P<0.01). Regardless of the microbial species, the cellulolytic bacteria seemed to inhibit the degradation of rice straw by rumen fungi. The high efficiency of fungal cellulolysis seems to affect bacterial degradation rates.

Characterization of Organochlorine Insecticide Endosulfan-Degrading Bacterium Isolated from Seaside Sediment (갯벌에서 분리한 유기염소계 살충제 Endosulfan 분해 세균의 특성)

  • Park, Mi-Eun;Kim, Young-Mog;Chung, Yong-Hyun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.44 no.3
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    • pp.207-215
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    • 2011
  • An endosulfan degrading bacterial strain, K-1321, was isolated by endosulfan-enrichment culture from a seaside sediment collected at Dadaepo Beach, Busan, Korea. The strain was identified as a Serratia sp. based on the results of morphological, biochemical and 16S rDNA homology analyses. Serratia sp. K-1321 was able to completely degrade 50 ppm endosulfan in culture media and soil within 6 weeks at $25^{\circ}C$. GC/MS analysis revealed that endosulfan diol was an intermediate of the bacterial endosulfan degradation. Considering the above results, we concluded that Serratia sp. K-1321 utilized endosulfan as a carbon source and metabolized endosulfan via a less toxic pathway, such as the formation of endosulfan diol as an intermediate.

Isolation of Antibiotic-producing Bacteria Antagonistic to Fusarium oxysporum from Sesame-growing Soils and Evaluation of Their Antifungal Activity

  • Lee, Yong Se;Ho Young Lee;Chang Ho Lee;Hee Sung Park
    • Journal of Microbiology and Biotechnology
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    • v.5 no.6
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    • pp.346-352
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    • 1995
  • For isolation of antibiotic-producing bacteria antagonistic to Fusarium oxysporum, a total of 327 microorganisms were screened from sesame-growing soils collected at various locations in Korea by the modified Herr's triple-agar-Iayer technique. Among the 36 bacterial isolates further screened by the dual culture test on tryptic soy agar, 10 were tested to show their antagonistic activity against 14 plant pathogenic fungi. Bacterial culture filtrates were shown either to inhibit some phytopathogenic fungal growth or to suppress F. oxysporum infection of sesame plants maintained in the green house. An isolate, B23, with the most prominent antagonistic activity was identified as Bacillus subtilis.

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Isolation and Identification of Antifungal Bacteria on Blue Mold in Apple (사과 푸른곰팡이병의 길항미생물의 분리 및 동정)

  • 이인선;조정일
    • Journal of Food Hygiene and Safety
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    • v.14 no.2
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    • pp.167-171
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    • 1999
  • In order to screen the antagonistic bacteria which inhibit the growth of the apple pathogen, Penicillum expansum, we isolated an effective bacterial strain and investigated into the antifungal activity of the antagonist and it's identification. The eleven strains of bacteria which strongly inhibited P. expansum were isolated from the nature, and the best antagonistic bacterial strain designated as CH142, was selected. The antagonistic strain CH142 was identified to be the genus Bacillus subtilis based on morphological and biochemical characterization. The CH142 showed 55.9% of antifungal activity against the growth of P. expansum. By the treatment of the culture broth and the heat treated culture filtrate of it, the B. subtilis CH142 showed 90% and 15% of antifungal activity, respectively.

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Idiopathic Nodular Panniculitis in a Poodle Dog (푸들개에서 발생한 특발성 결절성 피부지방염의 발생 예)

  • 박희명;오태호;정인성
    • Journal of Veterinary Clinics
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    • v.17 no.2
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    • pp.510-514
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    • 2000
  • A seven-year-old male poodle was presented with multiple non-pruritic draining skin lesions. On physical examination the dog was depressed and had three draining nodules 3.0 to 4.0 cm in diameter on the neck, flank and back. The multifocal sparing of fat lobules, macrophages, and numerous neutrophils were observed in aspiration cytologic examination of nodule. Haematological examination demonstrated a moderate leukocytosis and neutrophilia. An aerobic bacterial culture was undertaken from draining lesions on the flank. But there was no bacterial growth in aerobic culture. Therapy was initiated with oral cephalexin at a dose of 20 mg/kg, body weight three times daily and prednisolone at 1.0 mg/kg twice daily. The (lug returned to normal within 24 hours of treatment and its general attitute and appetite were improved. However, within 1 month new nodules had appeared on the dorsum over the rump, coalescing into a mass of draining nodules and tracts.

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Isolation of A Bacterial Strain for Fermentation of Omija Vinegar (오미자 식초 제조를 위한 식초산균 분리 및 동정)

  • Lim Yong-Suk;Sul Ill-Whan
    • Journal of the East Asian Society of Dietary Life
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    • v.14 no.5
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    • pp.508-512
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    • 2004
  • In other to produce vinegar using Schizandra chinensis Ballon(omija), acetic acid bacteria(AAB) were selected from several conventional vinegars, and total 30 acetic acid bacterial strains were isolated. Among the isolated strains, a strain was selected from medium containing omija juice which showed the highest productivity of acetic acid. The strain was identified as Acetobacter sp. C5-1b. Optimum conditions for acetic acid production of Acetabacter sp. C5-1b were involved with 30t: of fermentation temperature and shaking culture. The acidity of culture medium was reached to 5.3% after 8 days shaking cultivation at 30℃.

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