• Korean Journal of Breeding Science
• /
• v.40 no.2
• /
• pp.192-195
• /
• 2008

'Sinunbong 1' is a japonica rice cultivar developed from the cross between "Sangjubyeo", high yield cultivar and 'Unbong 17', cold tolerance at Honam Agricultural Research Institute (HARI), NICS, RDA, in 2005. This cultivar has a short grain shape and about 111 days growth duration from transplanting to harvesting in Korean climate condition. This cultivar shows high resistant reaction to the blast, but susceptible to bacterial blight and strip virus. This variety has cold tolerance, compared to Odaebyeo. The milled kernels of 'Sinunbong 1' are translucent with non-glutinous endosperm. It has about 19.2% of amylose content and better palatability of cooked rice compared with 'Odaebyeo'. The milled rice yield of 'Sinunbong 1' is about 5.46 MT/ha under the standard fertilizer level of the ordinary transplanting cultivation. 'Sinunbong 1' would be adaptable to the northern plain, mid-mountainous, southern mountainous of Korea.

• Korean Journal of Breeding Science
• /
• v.41 no.2
• /
• pp.158-162
• /
• 2009

"Wonkwang" is a new sprout-soybean cultivar developed from the cross between Danyeobkong and MS91001 at the Honam Agricultural Research Institute (HARI) and National Institute of Crop Science (NICS), RDA, in 2007. The preliminary, advanced and regional yield trials to evaluate the performance of Iksan 56 were carried out from 2003 to 2007. This cultivar has a determinate growth habit, purple flower, grayish brown pubescence, yellow seed coat, Grayish brown hilum, lanceolate leaflet shape and small seed size (10.9 g/100 seeds). The maturity date of "Wonkwang" is three days later than the check variety, "Pungsan". It has a good seed quality for soybean-sprout and resistance to lodging. The soybean-sprouts grown from "Wonkwang" have high isoflavone ($3,481{\mu}g/g$)contents. This cultivar has resistance to soybean mosaic virus (SMV) and necrotic symptom (SMV-N) and other most troublesome soybean diseases which are bacterial pustule and black root rot. The grain yield of "Wonkwang"in the regional yield trials (RYT) for 3 consecutive years was averaged 3.05 ton per hectare, which was 8% higher than that of the check cultivar "Pungsan".

• Korean Journal of Breeding Science
• /
• v.40 no.4
• /
• pp.447-451
• /
• 2008

'Goamibyeo', a mid-late maturing ecotype with high amylose content in kernels, was developed by the rice breeding team of National Yeongnam Agricultural Experiment Station(NYAES) in 2000 and released in 2001. This variety was derived from the three way cross of Milyang 95//Kimcheonaengmi/$2^*Ilpumbyeo$ (in 1992 summer) through the pedigree breeding method and designated as 'Milyang 168' in 1997. The heading date of 'Goamibyeo' was Aug. 18 in ordinary season with culm length of 85 cm. However, 'Goamibyeo' showed susceptible to bacterial leaf blight, stripe virus and leaf blast disease. The amylose content of 'Goamibyeo' in milled rice kernels is about 26.7% with translucent and clear in chalkness. Thus, 'Goamibyeo' is expected to be used as a source grain for rice noodle industries. The milled rice yield potential of 'Goamibyeo' is about 5.38 MT/ha in local adaptability test of three years and it would be adaptable to Yeongnam plain of Korea.

• Korean Journal of Breeding Science
• /
• v.41 no.4
• /
• pp.534-538
• /
• 2009

Japanese apricot (Prunus mume) "Okboseok" was released from a cross between "Nanko" and "Yoseibai" in 1993 at the Pear Research Station of National Institute of Horticultural and Herbal Science, Rural Development Administration. It was preliminarily selected in 2001 and named in 2006. It produces high yield with a flesh about average 88.3% of fruit, although the fruit size is small (approximately 14.0g). Horticultural and physiological harvest time of this cultivar is mid and late June respectively. It has single flowers with pink petal color and its flowering time is about 4 days later than that of "Nanko". Its fruit is tolerant to bacterial shot hole (Xanthomonas arboricola pv. Pruni) and pear scab (Venturia nashicola). "Okboseok" has abundant pollen grains (about 96 mg/100 flowers), thus it could be a pollinizer. To obtain stable yields, however, its compatibility with major cultivars is needed to be confirmed as pollinizer cultivar. "Okboseok" is recommended for being preserved in sugar and liquor.

• Korean Journal of Clinical Laboratory Science
• /
• v.50 no.4
• /
• pp.438-443
• /
• 2018

The emergence of biofilms have generated urgent alarm in clinical and medicine manufacturing fields engaged in the search for novel antimicrobials from ethno-medicinal plants. The National Institutes of Health (NIH) has estimated that 70% of all microbial infections in the world are associated with biofilms. In addition, the emergence of strains resistant to conventional antibiotics has become a serious threat to global public health. Therefore, finding alternative medicines is a major issue in the field of integrative medicine. In this study, four different herb extracts were screened for biofilm formation and the activities of Klebsiella pneumoniae. Of them, Scutellaria scordifolia Fisch. ex Schrank extracts had inhibitory effects on bacterial growth and biofilm formation. The Scutellaia scordifolia Fisch. ex Schrank extracts did not cause any cytotoxicity to L929 cells. The growth of K. pneumoniae was inhibited compared to other comparators in the experimental group containing Scutellaia scordifolia Fisch. ex Schrank. In a group of experiments with plant extracts, a maximum of 60 times the level of living bacteria was confirmed compared to the controls without the addition of the Scutellaia scordifolia Fisch. ex Schrank extracts. In a group of experiments with a significantly lower level of fluorescence extraction, differential interference contrast imaging showed that the number of K. pneumonae was reduced. These results suggest that extracts of this plant be applied as antimicrobial agents against K. pneumoniae, particularly in biofilm forms.

• Clean Technology
• /
• v.24 no.4
• /
• pp.293-300
• /
• 2018

Sulfamethoxazole (SMX) is sulfaamide-based synthetic antibiotics, which are widely prescribed pharmaceutical compound to treat bacterial infections in both human and animals. Most of them are not completely decomposed as refractory substances. The environmental impact of pharmaceuticals as emerging contaminants has generated severe concerns. In this study, catalytic wet peroxide oxidation (CWPO) of SMX was carried out with $Cu/Al_2O_3$ catalyst and investigated the optimum reaction conditions of temperature, dosage of catalyst and concentration of $H_2O_2$ to completely decompose the SMX. It was observed that SMX was completely decomposed within 20 min using 0.79 mM $H_2O_2$ and 6 g $Cu/Al_2O_3$ catalyst at 1 atm and $40^{\circ}C$, but SMX was not fully mineralized and converted to intermediates as hydroylated-SMX, sulfanilic acid, 4-aminobenzenesulfinic acid and nitrobenzene. After that these are completely mineralized through organic acid. We proposed the decomposition reaction path ways of SMX by analyzing the behavior of these intermediates. To investigate the durability of heterogeneous catalyst, decomposition of SMX was observed by continuously recycling catalysts. When the heterogeneous catalyst of 10 wt% $Cu/Al_2O_3$ was continuously reused 5 times, decomposition of SMX was a little lowered, but the activity of catalyst was overall very stable.

• Korean Journal of Microbiology
• /
• v.55 no.2
• /
• pp.131-142
• /
• 2019

The primary objective of this study was to determine the content of biogenic amines in Korean traditional fermented soybean pastes (doenjang) and to isolate potential probiotic Bacillus sp. with the ability to inhibit biogenic amines accumulation. There were significant differences in the bacterial cell counts, pH value, titratable acidity, salinity, and biogenic amine content between the samples. Among Bacillus strains isolated from doenjang, Bacillus (B.) licheniformis DB102, B. subtilis DB203, B. stearothermophilus DB206, Bacillus sp. DB209, Bacillus sp. DB310, B. coagulans DB311, B. cereus DB313, B. amyloliquefaciens DB714, Bacillus sp. DB917, B. cereus DB 915, B. subtilis DB1020, and Bacillus sp. DB1022 were found to be able to produce biogenic amines. On the other hand, biogenic amine-degrading strains were identified as Bacillus sp. DB403, Bacillus sp. DB407, B. subtilis DB517, B. licheniformis DB612, and B. subtilis DB821. In particular, Bacillus sp. DB407 and B. subtilis DB821 showed probiotic properties including tolerance to artificial digestive juices, adherence to intestinal epithelial cells, resistance to antibiotics, and antibacterial activity against biogenic amine-producing strains. In conclusion, the two probiotic Bacillus strains may be considered as the suitable starter for manufacture of fermented soybean foods with low biogenic amines content.

• Journal of Korean society of Dental Hygiene
• /
• v.19 no.3
• /
• pp.387-397
• /
• 2019

Objectives: This study was conducted to examine the subjects' oral health status and changes in the oral environment after mouthwash with effervescent Vitamin C and its intake and to understand the impact on changes in the number of bacteria causing periodontal diseases in the oral cavity based on the oral health status and oral environmental condition. Methods: After obtaining approval from the Institutional Review Board of D-University, 45 people (24 in an experimental group and 21 in a control group) who participated in the oral health program were selected, among visitors of the Clinical Dental Hygienics Laboratory of D. University in B. Results: Based on the subjects' periodontal health status, the number of bacteria causing periodontal diseases in the oral cavity decreased in both experimental and control groups before and after the experiment. However, in the experimental group, it was significant when the Bleeding on Probing(BOP) level was below average, and Calculus index(CAL) was above average, in Aggregatibacter actinomycetemcomitans and the red complex. It was also significant when CAL was above average in the orange complex in the control group. Regarding bacterial changes in periodontal diseases based on the subjects' oral environmental condition, there was a significant change in the Adenosine Tri Phosphate (ATP : intraoral contaminants test) test in the oral cavity in A. actinomycetemcomitans and the red complex in the experimental group and in the O'Leary index in the control group. Further, there was a significant change in the orange complex in the ATP test in the experimental group. Conclusions: A comprehensive analysis of the results revealed that the oral health care program for dental hygiene decreased the number of bacteria causing periodontal diseases in the oral cavity in both experimental and control groups.

• Journal of Life Science
• /
• v.29 no.6
• /
• pp.712-723
• /
• 2019

Chemically synthesized compounds are widely used in oral hygiene products. However, excessively long-term use of these chemicals can cause undesirable side effects such as bacterial tolerance, allergy, and tooth discoloration. To solve these issues, significant effort is put into the search for natural antibacterial agents. The aim of this study was to assess the extracts of foreign native plants that inhibit the growth and biofilm formation of Streptococcus mutans. Among the 300 foreign plant extracts used in this study, Chesneya nubigena (D. Don) Ali extract had the highest antimicrobial activity relatively against S. mutans with a clear zone of 9 mm when compared to others. This plant extract also showed anti-biofilm activity and bacteriostatic effect (minimal bactericidal concentration [MBC], 1.5 mg/ml). In addition, the plant extracts of 19 species decreased the ability of S. mutans to form biofilm at least a 6-fold in proportion to the tested concentrations. Of particular note, C. nubigena (D. Don) Ali extract was found to inhibit biofilm formation at the lowest concentration tested effectively. Therefore, our results reveal that C. nubigena (D. Don) Ali extract is a potential candidate for the development of antimicrobial substitutes, which might be effective for caries control as well, as demonstrated by its inhibitory effect on the persistence and pathogenesis of S. mutans.

• Journal of Life Science
• /
• v.29 no.6
• /
• pp.631-636
• /
• 2019

A new nitric oxide-induced (NOI) gene was isolated by screening ESTs from a cDNA library of dehydration-treated fibrous roots of sweetpotato (Ipomoea batatas). The 720 bp cDNA fragment, IbNOI, was sequenced, from which a 77 amino acid residue protein was deduced. A search of the protein BLAST database identified significant similarity to other plant NOI protein sequences. Quantitative RT-PCR analysis revealed diverse expression patterns of IbNOI in various tissues of the intact sweetpotato plant, and in leaves exposed to different stresses. The IbNOI gene was highly expressed in storage roots and suspension-cultured cells. In leaf tissues, IbNOI showed strong expression during sodium nitroprusside (SNP)-induced NO accumulation and chemical stress treatments. Expression of IbNOI was also induced under various abiotic stress conditions, such as dehydration, salt, and bacterial pathogen infection. These results suggest that IbNOI is involved in plant responses to diverse abiotic stresses and pathogen infection through a NO-related pathway.