• Journal of Veterinary Science
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• 제23권1호
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• pp.6.1-6.8
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• 2022

Background: With limited information available, the association among urinary tract infections, urease-producing bacteria and the presence of magnesium ammonium phosphate (MAP) urolithiasis in canines in Thailand requires more study. Objectives: This study aimed to investigate the association between demographic characteristics of canines and the presence of MAP urolithiasis in canines, and to evaluate antimicrobial susceptibility patterns of bacteria isolated from canine uroliths. Methods: A total of 56 canines admitted for treatment with surgical removal of uroliths were recruited. Demographic characteristics and clinical chemistry data were recorded. Bacteria isolated from the removed uroliths were identified. Chemical compositions of the uroliths were analyzed by Fourier transform infrared spectrometer. Potential risk factors were determined with univariable and multivariable logistic regression analyses. Results: Of 56 canine urolithiasis, bacteria were isolated from uroliths of 38 canines (27 MAP and 11 non-MAP) but not from uroliths of 18 canines (5 MAP and 13 non-MAP). The most common bacteria found in nidus of MAP uroliths was Staphylococcus pseudintermedius (approximately 51%). An antimicrobial resistance was frequently found in Staphylococci isolates (42.86%). Multivariate logistic regression analysis showed that the predictors of MAP urolith in canine urolithiasis were being female (p = 0.044; adjusted odds ratio [OR], 10.22; 95% confidence interval [CI], 1.06-98.24) and the positive urolith culture (p = 0.012; adjusted OR, 8.60; 95% CI, 1.60-46.30). Conclusions: Our results indicate that S. pseudintermedius (a urease-producing bacterium) is the major causative bacteria of MAP uroliths. A positive urolith culture and being female are risk factors of MAP urolithiasis in canines.

• 미생물학회지
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• 제38권4호
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• pp.281-286
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• 2002

2000년 1 월부터 2000년 10월까지 가두리 양식장에서 Vibrio vulnificus를 검출하였고 이들의 억제 방법을 연구하였다. 이 세균의 검출은 선택적 분리법과 vvhA 유전자를 확인하는 방법을 이용하였다. V. vulnificus는 수온이 $17^{\circ}C$이상인 5월부터 검출되었고 $19^{\circ}C$ 이상인 6월부터 9월까지는 대부분의 시료에서 검출되었다. V. vulnificus를 제어하기 위한 방법 중 냉동 및 냉장 처리는 살균효과를 나타내지 못했다. Citric acid도 균의 생장을 억제하지 못했으나, 500 mg/1이상의 EDTA가 첨가될 경우 균이 완전히 사멸되었다. 분말 광촉매인 산화티타니움은 자외선을 조사할 경우 15분～1 시간 이내에 이 세균을 완전히 사멸시키는 효과를 나타내었다. 산화티타니움을 유리 구슬에 코팅한 광촉매 장치를 이용하여 0.2/min의 turnover rate로 사멸효과를 얻었다.

• 한국식품위생안전성학회지
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• 제7권2호
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• pp.107.2-119
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• 1992

Fish pathology is one of the main scientific bases upon which this expansion in aquaculture has been dependent and requires a wide knowledge of the environmental constraints, the physiology and characteristic of the various pathogens, the responses of the host, and the methods by which they may be controlled. The primary disease and parasite problems in aquaculture animals related to viral, bacteria, fungal and protozoan epizootics. Parasitic nematodes, trematodes and cestodes are commonly found in aquaculture animals, but seldom are they present in concentrations sufficient to cause significant problems, When an epizootic does occur and chemical treatment is indicated, the appropriate chemical must be selected an properly applied. We have antibiotics, sulfa, nitrofuran and other chemicals for treatment of fish diseases, Some may be mixed with the feed during formulation, added to the pellets of feed as a surface coating, given in the form of an injection or used as a bath. Even though a drug or chemical has been officially approved for use in aquaculture, the substance should never be used unless there is a clear need, Some of the reasions for this view are as follows: (1) the constant use of antibiotics can leak to the development of resistant strains of bacteria, (2) biofilter efficiency may be impaired or destroyed by chemicals added to closed recirculating water systems, and(3) the injudicious use of chemical can have a damaging effect on the environment as well as on human.

• 대한치위생과학회지
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• 제5권2호
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• pp.155-164
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• 2022

Background: By regulating the factors that contribute to oral diseases in a healthy way, oral health can be maintained and prevented. Methods: The general characteristics, PHP index, oral health behavior, and clinical periodontal index of each group were calculated by frequency analysis, and a cross-analysis (χ² test) was conducted to assess the homogeneity of the general characteristics, oral health behavior, and clinical periodontal index of the study subjects. An Oral bacteriological examination was performed by gargling with saliva. Results: The expert periodontal prevention group showed a decrease in the copy number of periodontal disease causative bacteria, and A. actinomycetemcomitans, P. gingivalis, T. forsythus, andT. denticolashowed a significant difference before and after treatment (p=0.021). In the periodontal treatment group, A. actinomycetemcomitans, P. gingivalis, T. forsythus, andT. denticolaall showed a decrease in copy number, but there was no significant difference. Conclusions: This study showed professional periodontal prevention management had some effect on periodontal bacterial reduction.

• 한국지반환경공학회 논문집
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• 제13권5호
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• pp.51-57
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• 2012

본 연구에서는 친환경적인 그라우트재의 개발을 위하여 연약지반에 대한 바이오그라우트 가능성을 확인하고, Bacillus Pasteurii 균을 이용하여 탄산칼슘 침전 효과를 분석하였다. 연약지반에 미생물의 탄산칼슘 침전을 이용하여 바이오그라우트에 미치는 영향을 알아보기 위해 4가지 시료의 조건(멸균 시료, 비멸균 시료, 반응용액과 미생물용액의 선처리 혼합시료, 반응용액과 미생물용액의 후처리 혼합시료)으로 실험되었다. 전자현미경(SEM), EDX와 X선 분석 회절기(XRD)를 이용하여 연약지반 시료의 분석을 수행하였고, 이러한 연구결과를 바탕으로 탄산칼슘 침전을 이용한 미생물 처리 공법은 바이오그라우트의 특성을 개선하였다.

• 상하수도학회지
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• 제22권4호
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• pp.449-454
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• 2008

Some pretreatment methods have been proposed to enhance the biodegradability and to shorten the hydrolysis reaction time. By means of efficient pretreatment the suspended solids (SS) can be made of better accessible for the anaerobic bacteria. There are several ways how this can be accomplished, which include biological, mechanical, thermal, and chemical methods. For the sludge solubilization using the cavitation phenomenon, we have tried to develop a pretreatment process consisted of a reactor and pumps. The objectives of this study were to develop a advanced wastewater treatment consisted of IABR and the cavitation with PGA. The most effective removal for organic matter and nutrients were occured when both cavitation pretreatment and ${\gamma}$-PGA were applied at the IABR process. Only small portion of ${\gamma}$-PGA at a rate of 1.38mg/L, was enough to improve sedimentation ability, SS removal efficiencies, and sludge volume reduction. After the sludge solubilization by the cavitation, SCOD increased to 193% and SS decreased to 36%. The removal ratio of BOD was 94.5%, T-N removal ratio was 85.5% and T-P removal ratio was 84.9%. The combination process of the IABR with the cavitation and PGA addition seems to be very effective alternative wastewater treatment process.

• IMMUNE NETWORK
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• 제8권3호
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• pp.67-74
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• 2008

Background: The effects of the dietary administration of two heat-inactivated whole bacteria from the Vibrionaceae family, singly or combined, on innate immune response of the rainbow trout were studied. The two bacteria (Pdp11 and 51M6), which were obtained from the skin of rainbow trout, showed in vitro characteristics that suggested they could be considered as potential fish probiotics. Methods: The fish were fed four different diets: control (non-supplemented), or diets supplemented with heat-inactivated bacteria at $10^8$ cfu/g Pdp11, $10^8$ cfu/g 51M6 or with $0.5{\times}10^8$ cfu/g Pdp11 plus $0.5{\times}10^8$ cfu/g 51M6 for 4 weeks. Six fish were sampled at weeks 1, 2, 3 and 4, and then the main humoral (natural haemolytic complement activity and serum peroxidase content) and cellular innate immune responses (leucocyte peroxidase content, phagocytosis, respiratory burst and cytotoxicity) were evaluated. Results: The serum peroxidase content and the natural haemolytic complement activity increased with time, reaching the highest values in the third and fourth weeks of feeding, respectively. The phagocytic ability of specimens fed the mixture of the two inactivated bacteria was significantly higher than in the controls after 2 and 3 weeks of treatment. The same activity increased significantly in rainbow trout fed the Pdp11 diet for 2 weeks or the 51M6 diet for 3 weeks. Respiratory burst activity was unaffected by all the experimental diets at all times assayed. Cytotoxic activity had significantly increased after 3 weeks in fish fed the 51M6 diet. Conclusion: Our results demonstrated the usefulness of incorporating inactivated probiotic bacteria into fish diets.

• 한국미생물·생명공학회지
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• 제48권2호
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• pp.193-204
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• 2020

Sixteen acetic acid bacteria (AAB) were isolated from blueberries and citric fruits of the Salto Grande region (Concordia, Entre Rios, Argentina) using enrichment techniques and plate isolation. Enrichment broths containing ethanol and acetic acid enabled maximum AAB recovery, since these components promote their growth. Biochemical tests allowed classification of the bacteria at genus level. PCR-RFLP of the 16S rRNA and PCR-RFLP of the 16S-23S rRNA intergenic spacer allowed further classification at the species level; this required treatment of the amplified products of 16S and 16S-23S ITS ribosomal genes with the following restriction enzymes: AluI, RsaI, HaeIII, MspI, TaqI, CfoI, and Tru9I. C7, C8, A80, A160, and A180 isolates were identified as Gluconobacter frateurii; C1, C2, C3, C4, C5, C6, A70, and A210 isolates as Acetobacter pasteurianus; A50 and A140 isolates as Acetobacter tropicalis; and C9 isolate as Acetobacter syzygii. The bacteria identified by 16S rRNA PCR-RFLP were validated by 16S-23S PCR-RFLP; however, the C1 isolate showed different restriction patterns during identification and validation. Partial sequencing of the 16S gene resolved the discrepancy.

• 한국환경과학회지
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• 제16권2호
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• pp.227-232
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• 2007

Water pollution in enclosed water bodies such as lake and river has become a serious problem over the world. Domestic wastewater is responsible for more than 60 % pollution load in public water area in Korea. Effluent of the treated domestic wastewater at low removal level is abundantly fed rivers and lakes and thus be an serious cause of lake pollution. Therefore, effective implement of domestic wastewater treatment in basin of lake and river must be prepared. The septic tank is one of the effective domestic wastewater treatment equipment and used in individual treatment for a unit of household, The purpose of septic tank as biological treatment system is simultaneously to remove BOD, T-N, T-P and reduce turbidity from influent. Accordingly, the appropriate control of functional microorganisms is important subject for the establishment of stability and economy of the biological treatment method. Especially, microanimals as a high-ranked microorganisms of food-chain are important, because microanimals control the other microorganisms especially various bacteria and effect on function of treatment. Therefore, it is necessary that functional predator like rotifers are attached in wastewater treatment process. In this study, the methods for attachment high density the rotifer to and improvement of transparency in the effluence by a dense rotifer was examined using laboratory scale biological treatment reactor simulated septic tank and real one.

• 대한한의학회지
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• 제26권1호
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• pp.195-205
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• 2005

Objective & Methods: To evaluate the in vitro synergic effect of Eunkyo-san, a traditional poly-herbal formula used in the treatment of respiratory diseases in oriental medicine with quinolone antibiotics, represented by ciprofloxacin (CPFX), which was used in the minimal concentration (MIC), $MIC_{50}$ and $MIC_{90}$. of single use of quinolones in concomitant treatment with Eunkyo-san against 9 strain$ of gram positive bacteria. Results: In. the case of aerobic gram positive bacteria, the MIC, $MIC_{50}$ and $MIC_{90}$ against Staphylococcus aureus, Staphylococcus aureus smith, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus pneumoniae Type I, Type II and Type III were significantly decreased in concomitant treated groups with Eunkyo-san compared to those of single treated groups of CPFX, respectively. However, no significant changes were demonstrated against Bacillus subtilis and Enterococcus faecalis. Conclusion: The in vitro antibacterial activity of CPFX were increased against some strains of gram positive strains, especially, pneumococcus such as Staphylococcus and Streptococcus, by concomitant use of Eunkyo-san.