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Molecular characterization and immunohistochemical localization of a mitogen-activated protein kinase, Accp38b, from Apis cerana cerana

  • Zhang, Liang;Meng, Fei;Li, Yuzhen;Kang, Mingjiang;Guo, Xingqi;Xu, Baohua
    • BMB Reports
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    • v.45 no.5
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    • pp.293-298
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    • 2012
  • The p38 mitogen-activated protein kinase (MAPK) is involved in various processes, including stress responses, development, and differentiation. However, little information on p38 MAPK in insects is available. In this study, a p38 MAPK gene, $Accp38b$, was isolated from $Apis$ $cerana$ $cerana$ and characterized. The quantitative real-time PCR (Q-PCR) analysis revealed that $Accp38b$ was induced by multiple stressors. Notably, the expression of $Accp38b$ was relatively higher in the pupae phase than in other developmental phases. During the pupae phase, Accp38b expression was higher in the thorax than in the head and abdomen and higher in the fat body than in the muscle and midgut. Immunohistochemisty showed significant positive staining of Accp38b in sections from the brain, eyes, fat body, and midgut of $A.$ $cerana$ $cerana$. These results suggest that Accp38b may play a crucial role in stress responses and have multiple aspects function during development.

Numerical calculation of Laminar flow in a Square Duct of 90° Bend (정사각형 단면을 갖는 90° 곡관의 층류유동 계산)

  • Kim H. T.;Kim J. J.
    • Journal of computational fluids engineering
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    • v.2 no.1
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    • pp.1-7
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    • 1997
  • A FA-FD hybrid method, developed for solving three-dimensional incompressible Navier-Stokes equations, is applied to calculate three-dimensional laminar flows through a square duct with a 90° bend. The method discretizes the convective terms in the primary flow direction with 3rd-order upwind finite-differences and the convective and diffusive terms in the transverse directions with the two-dimensional finite analytic method. The non-staggered grid system is used and the pressure-velocity coupling is achieved by a global iteration procedure based on the PISO algorithm. Detailed comparisons between the computed solutions and the available experimental data are given mainly for the velocity distributions at cross-sections in a 90° bend of a square duct with both fully developed and developing entry flows. Although the computational result shows generally a good agreement with the experimental data, there are some significant discrepancies underlining the necessity of more accurate numerical methods as well as reliable experimental data for their validation.

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A Study on the Purchasing Cost Reduction -through Value Analysis Technique- (구매원가절감에 관한 연구 (VA 활동을 중심으로))

  • 여동길
    • Journal of Korean Society of Industrial and Systems Engineering
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    • v.2 no.2
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    • pp.11-18
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    • 1979
  • The purpose of this study is to establish a purchasing cost reduction program for the management practical use. The research method of this study is a bibliographical search of the materials available in the library at Keimyung University and the writer's private study, and the case analysis. The body of this study is divided into three sections. The first section deals with the purchasing management systems in terms of the nature and characters of purchasing management and purchasing cost in total management system. The second section includes purchasing cost reduction program through value analysis technique and the third section covers summary and the following conclusions : 1) Top management should be aware of the important position of purchasing activities from the cost point of view, and they should be aware that a good purchasing cost reduction program could be established through value analysis technique. 2) For such a purchasing cost reduction program to be carried out. effectively the management should support and back up the program. 3) There should be a training program to educate and develop the personnel who can manage and operate the purchasing cost reduction program through valuean analysis technique. 4) And cooperative system should be established in management and operative organization system from the total management systems, view for the effective use of the program.

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Estimation of Performance and Pavement Life using National Highway Pavement Condition Index (NHPCI 지표를 활용한 공용성 추정과 수명 산정)

  • Do, Myung-Sik;Lee, Yong-Jun;Lim, Kwang-Su;Kwon, Soo-Ahn
    • International Journal of Highway Engineering
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    • v.14 no.5
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    • pp.11-19
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    • 2012
  • PURPOSES: The new methodology is proposed for estimation of long-term performance and pavement life based on the national highway database in Daejeon area. Furthermore, this study tried to verify the applicability of performance estimation using NHPCI (National Highway Pavement Condition Index) on tendency of pavement deterioration as time goes by under Korean road environments. METHODS: Reliability theories are applied to estimate the mean life and to determine the appropriate distribution using 3 levels of traffic loads (high, medium, low) based on maintenance and rehabilitation history data for 15 years. RESULTS: As a result, Lognormal distribution is suitable for explanation of pavement lifetime in Daejeon area regardless of traffic loads. In addition, we found that the results of mean life and maintenance timing based on NHPCI for the pavement sections of 3 levels of traffic loads are available. CONCLUSIONS: Based on this study, it was found that mean life of high, medium and low levels of traffic loads are about 8.1 years, 12.2 years and 12.7 years, respectively. Higher level of traffic loads shorten the pavement mean life.

Wind Turbine Airfoils considering Surface Roughness Effects (표면거칠기 둔감도를 고려한 풍력발전기용 익형 개발)

  • Kim, Seok-Woo;Shin, Hyung-Ki;Jang, Moon-Seok
    • New & Renewable Energy
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    • v.3 no.3
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    • pp.36-44
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    • 2007
  • Most airfoils for wind turbines commercially available have been developed for aircrafts, which are operated at high Reynolds numbers. However, Reynolds numbers of wind turbines are very low compared to those of aircrafts. In other to improve wind turbine performances, airfoils for the use of wind turbine shall be designed such as S-series airfoils developed by NREL in America. The authors have designed new airfoils for wind turbines considering designated operation conditions of wind turbines and even local wind resources in Korea. The designed airfoils are characterized by improved roughness insensitivities compared to other airfoils such as S814 and S820. The developed KWA005-240 and KWA009-127 are for root and tip sections of a wind turbine blade, respectively. Although the results show much improved performances against NACA airfoils, performance data of post-stall regulation loses some accuracies due to the characteristics of the simulation tool of XFOIL. Therefore, wind tunnel experiments are required for more accurate evaluation of the designed airfoils. Currently, the experiments has been completed and the data analysis works are going on now. The final results obtained from the experiments will be published soon.

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Analysis Model for Predicting the Flexural Behavior of RC Beam Strengthened with FRP (FRP로 보강된 RC보의 휨거동 예측을 위한 해석모델)

  • Hong, Ki-Nam;Lee, Bong-No;Han, Sang-Hoon
    • Journal of the Korean Society of Safety
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    • v.26 no.2
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    • pp.62-69
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    • 2011
  • This paper presents a new simple two-dimensional frame finite element able to accurately estimate the load-carrying capacity of reinforced concrete beams flexurally strengthened externally bonded fiber reinforced polymer (FRP) strips and plates. The proposed analysis model considers distributed plasticity with layer-discretization of the cross-sections and the bond-slip behavior of epoxy layer. The proposed model is used to predict the load-carrying capacity and the applied load-midspan deflection response of RC beams subjected to bending loading. Numerical simulations and experimental measurements are compared based on numerous tests available in the literature and published by different authors. The numerically simulated response agree remarkably well with the corresponding experimental results. Thus, the proposed model is suitable for efficient and accurate modeling and analysis of flexural strengthening of RC beams with externally bonded FRP sheets/plates and for practical use in design-oriented parametric studies.

Scenario Analysis of Flood Travel Time using Hydraulic Model in Downstream of Nakdong River (수리학적 모형을 이용한 낙동강 하류구간에서의 홍수도달시간 시나리오 분석)

  • Choi, Hyungu;Lee, Eulrae
    • Journal of Korea Water Resources Association
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    • v.48 no.3
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    • pp.197-207
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    • 2015
  • Modification of travel time is necessary in all Nakdong river basin because hydrological conditions of Nakdong river basin were changed after major rivers project. Also calculation of flood travel time at between sections of weirs is necessary. In this study, flood travel time was calculated using hydraulic model and the latest topographical data from Changnyeong-Haman weir to Nakdong river estuary bank. Analysis of discharge and stage conditions were carried out. 84 of the scenarios were organized according to flow rate, discharge type, boundary conditions, and tributary conditions. Flood travel time of initial and peak were calculated with discharge and stage conditions, respectively. The results of this study will be available in practical business work such as flood forecast warning and weir operation on algae removal.

A Study on Constant-Speed Position Control of Solid Freeform Fabrication System (임의형상가공시스템의 정속위치제어)

  • Jung, Yong-Rae;Ko, Min-Kook;Kim, Seung-Woo
    • Proceedings of the KIEE Conference
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    • 2002.11c
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    • pp.75-78
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    • 2002
  • SFFS(Solid Freeform Fabrication System) is commercializing to rapid prototyping concept in world-wide some corporations including the States, have much technological problems yet and need new mode for agile solid freeform fabrication as well as prototyping. In this paper, we design an automatic control algorithm that the cutting path of laser beam, on the SFFS, is controlled with constant speed. The designed algorithm for constant-speed path control is implemented and experimented in the $CAFL^{VM}$ (Computer Aided Fabrication of Lamination for Various Material) system, the new SFFS which is developed in this paper. Its process is an automated fabrication method in which a 3D object is constructed from STL(SToreoLithography) 2D data, derived from CAD 3D image, by sequentially laminating the part cross-sections. The constant-speed path control is started from the STL data. After STL file is modified in data format to be available for control. The fabrication of the 2D part is, with constant speed, conducted from the 23 position data by laser beam. we confirm its high-performance through experiment results from the application into $CAFL^{VM}$ system.

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A Study on Quantitative Lateral Drift Control of Tall Steel Braced Frames subject to Horizontal Loads (수평하중을 받는 고층철골가새골조의 정량적인 횡변위제어에 관한 연구)

  • Kim, Ho-Soo;Lee, Han-Joo
    • Journal of Korean Society of Steel Construction
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    • v.12 no.4 s.47
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    • pp.397-406
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    • 2000
  • This study presents an effective optimal technique to control quantitatively lateral drift for tall steel braced frames subject to horizontal loads. In this paper, the displacement sensitivity depending on behavior characteristics of steel braced frames is established, and also the approximation concept that has the generality of the mathematical programming and can efficiently solve large scale problems is introduced. Especially, the commercially available standard steel sections are used for the discrete selection of member sizes. Three types of 12-story braced frames and a 30-story braced framework are presented to illustrate the features of the quantitative lateral drift control technique proposed in this study.

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식육의 연화증진에 관한 최근의 연구동향 - 적색육의 연도 증진에 이용되는 전기자극의 작용 -

  • 황인호
    • Proceedings of the Korean Society for Food Science of Animal Resources Conference
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    • 2002.05a
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    • pp.17-25
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    • 2002
  • Application of electrical stimulation in the red meat species (eg. beef and sheep) processing has been erratic around the world and this may reflect an incomplete knowledge of how to optimise the technology. Although it is well established that stimulation increases the rate of post-mortem glycolysis other biochemical and biophysical effects have been implicated with the use of this technology. On the basis of currently available knowledge, this mini-review seeks to examine the current theories about the effect of stimulation on post-mortem muscle. The classical view that stimulation prevents muscle from shortening excessively during rigor development has been expanded to include the possibility that it also results in physical disruption of muscle structure. The interaction of these effects with the acceleration of the rate of proteolysis through activation of the calpain protease system has not been comprehensively reviewed in the past. As a result of conclusion driven, this article highlights several areas that may prove fruitful for further research. The challenge for further development of electrical stimulation systems is optimisation of the activation of the enzyme systems in parallel with manipulation of chilling regimes so as to ensure rigor mortis is achieved at temperatures which minimise shortening. The potential of regional stimulation of sections of the carcass to achieve this outcome is worthy of study given the different fibre composition of muscles and temperature gradients.

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