This study investigates the effect of ${\beta}$-glucans on the growth of Caenorhabditis elegans. Comparison was made among lipopolysaccharide (LPS) and ${\beta}$-glucans extracted from Phellinus baumii, in the presence of peptidoglycans which is available as the major carbon source from OP50, a non-pathogenic strain of Escherichia coli. When the three sources of carbohydrate were added singularly or in mixture to the culture media, a significant level of variation was observed with respect to fecundity. Addition of ${\beta}$-glucans appeared to increase the fecundity. When ${\beta}$-glucans was reinforced in the culture media, the fecundity increased at least 20 percent compared to the OP50-only media which exclusively contains peptidoglycans. In terms of life span, C. elegans showed a modest reduction when treated especially with ${\beta}$-glucans. C. elegans accumulated less fat in the ${\beta}$-glucans containing media different from the OP50 media. Based on the Sudan black staining, fat deposition significantly decreased corresponding to the ${\beta}$-glucans content in the media. On LPS-supplemented media, no difference was observed in fat deposition compared to the normal OP50 media. At the level of motility, ${\beta}$-glucans-treated worms moved more distance as well as LPS-treated worm. They also showed a comparable degree of motility under similar treatment with each source of carbohydrate. In conclusion, LPS and ${\beta}$-glucans, extracted from P. baumii, may not entirely replace the food required for C. elegans; however, it might be utilized as valuable alternative food source which C. elegans use as forms of carbohydrates in stead of peptidoglycan of OP50.
This study was conducted to investigate the responses of soil properties and microbial communities to different agricultural management and soil types, including organic management in Andisols (Org-A), organic management in Non-andisols (Org-NA), conventional management in Andisols (Con-A) and conventional management in Non-andisols (Con-NA) by using a pyrosequencing approach of 16S rRNA gene amplicon in Radish farms of volcanic ash soil in Jeju island. The results showed that agricultural management systems had a little influence on the soil chemical properties but had significant influence on microbial communities. In addition, soil types had significant influences on both the soil chemical properties and microbial communities. Organic farming increased the microbial density of bacteria and biomass C compared to conventional farming, regardless of soil types. Additionally, Org-NA had the highest dehydrogenase activity among treatments, whereas no difference was found between Org-A, Con-A and Con-NA and had the highest species richness (Chao 1) and diversity (Phyrogenetic diversity). Particularly, Chao 1 and Phyrogenetic diversity were increased in organic plots by 12% and 20%, compared with conventional plots, respectively. Also, regardless of agricultural management and soil types, Proteobacteria was the most abundant bacterial phylum, accounting for 21.9-25.9% of the bacterial 16S rRNAs. The relative abundance of putative copiotroph such as Firmicutes was highest in Org-NA plot by 21.0%, as follows Con-NA (13.1%), Con-A (6.7%) and Org-A (5.1%.), respectively and those of putative oligotrophs such as Acidobacteria and Planctomycetes were higher in Con-A than those in the other plots. Furthermore, LEfSe indicated that organic system enhanced the abundance of Fumicutes, while conventional system increased the abundance of Acidobacteria, especially in Non-andisols. Correlation analysis showed that total organic carbon (TOC) and nutrient levels (e.g. available P and exchangeable K) were significantly correlated to the structure of the microbial community and microbial activity. Overall, our results showed that the continuous organic farming systems without chemical materials, as well as the soil types made by long-term environmental factors might influence on soil properties and increase microbial abundances and diversity.
Kim, Myung-Seok;Park, Jang-Hyun;Chung, Byung-Jun;Park, Gyu-Chul;Park, Tae-Dong;Kim, Sang-Chul;Shim, Jae-Han
Korean Journal of Medicinal Crop Science
/
v.9
no.2
/
pp.91-98
/
2001
Scutellaria baicalensis G. was cultivated in plots of different soil textures which were sand loam(SL), loam(L) and clay loam(CL). Also three fertilizer conditions were used; non application (NA)treatment, rice straw manure(RSM) treatment and N-P-K, chemical fertilization(CF) treatment. The chemical-physical properties, such as organic matter, available phosphate, $K_2O$, CaO, clay contents and porosity ratio in CL plot with RSM application were the most proper in CL plot and RSM application for the culture of S. baicalensis plants. RSM had very high contents of total nitrogen, 2.25% and C/N ratios, 21.4. Thus the growth of shoot and root in loam plot with RSM treatment were greater compared to that of CL plot with NA treatment. Whereas, The highest baicalin, baicalein and wogonin contents in roots were found in CL plot with RSM treatment. There was significantly positive correlation between aerial and underground parts of plant, yield and contents of T-N, $K_2O$ but negatively correlated with the contents of baicalin, baicalein and wogonin in S. baicalensis roots.
Magazine of the Korean Society of Agricultural Engineers
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v.16
no.1
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pp.3293-3301
/
1974
An experimental work was conducted by using a laboratory-made model dryer to investigate the effect of the rate of natural forced-air on the drying rate of rough rice which was deposited in the deep-bed. The dryer consisted of 8 cylinderical containers with grain holding screen at their bottoms, each of which having 30cm in diameter and 15cm in height. The containers were sacked vertically with keeping them air-tight by using paper tape during dryer operation. Two separate layers of containers were operated in the same time to have two replications. The moisture contents of grains within each bins after predetermined period of dryer operation were determined indirectly by measuring the weight of the individual containers. The air-rates were maintained at 6 levels, or 5, 8, 10, 15, 18 and 20 millimenters of static head of water. The roomair conditions during dryer operation were maintained in the range of 10-l5$^{\circ}C$ in temperature and 40-60% in relative humidity. The results of the study are summarized as follows: 1. Drying characteristics of the grains in the bottom layers were approximately the same regardless of airdelivery rates, giving the average drying rate as about 0.35 percent per hour after 40-hour drying period, during which moisture content (w. b.) reduced from 24 percent to about 10 percent. 2. After about 40-hour drying period, the mean drying rates increased from 0.163 percent per hour to 0.263 percent per hour as air-flow rates increased from 5mm to 87.16mm of static head of water. In the same time, the moisture differences of grains between lower and upper layers varied from 12.7 percent at the air rate of 5mm of water head to 7.5 percent at the air-flow rate of 20mn of water head. Thus, the greater the air-flow rate was, the more overall improvement in drying performance was. Additionally, from the result of ineffectiveness of drying grain positioned at 70cm depth or above by the air rate of 5mm of static head of water it may be suggested in practical application that the height of grain deposit would be maintained adequately within the limits of air-rates that may be actually delivered. 3. Drying after layer-turning operation was continued for about 30 hours to test the effectiveness of reducing moisture differences in the thick layers. As a result of this layer-turning operation, moisture distribution through layers approached to narrow ranges, giving the moisture range as about 7 percent at air-flow rate of 5mm head of water, about 3 percent at 10mm head about 2 percent at 15mm head, and less than 1 percent at 20mm head. In addition, from the desirable results that drying rate was rapid in the lower layers and dully in the upper layers, layer-turning operation may be very effective in natural air drying with deep-layer grain deposit, especially when the forced air was kept in low rate. 4. Even though the high rate of air delivery is very desirable for deep-layer natural-air drying of rough rice, it can be happened that the required air delivery rate could not be attained because of limitation of power source available on farms. To give a guide line for the practical application, the power required to perform the drying with the specified air rate was analyzed for different sizes of drying bin and is given in Table (5). If a farmer selects a motor of which size is 1 or {{{{1 { 1} over {2 } }}}} H.P. and air-delivery rate which ranges from 8~10mm of head, the diameter of grain bin may be suggested to choose about 2.4m, also power tiller or other moderate size of prime motor may be recommended when the diameter of grain bin is about 5.0m or more for about 120cm grain deposit.
Kim, Sang-Youb;Oh, Hyun-Woo;Moon, Doo-Khil;Han, Hae-Ryong;Chung, Jong-Bae
Korean Journal of Environmental Agriculture
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v.17
no.2
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pp.174-181
/
1998
In four citrus grow of Satsuma mandarin (rootstock of trifoliate orange) including two grove of organical management and two groves of conventional management, spores of arbuscular mycorrhizal(AM) fungi were identified and seasonal changes in spore density in soils and AM colonization of citrus roots were investigated. AM colonization in weeds found in the groves were also examined. Three species of Glomus (G.deserticola, G. vesiculiferum, G. rubiforme ) and one unknown species of Acaulospora were observed in all of the groves. Annual mean density of AM fungal spores were in the range of 10,000${\sim}$40,000 per 100g soil with more spores in the organically-managed groves. The least spores were observed in December in all groves, and the most spores in April in the organically-managed groves while in February or April in the conventionally- managed. Annual mean AM colonization more 27% of citrus root were observed in the organically-managed with the high peaks in April and October and the minimum in August, while mean colonization less than 15% in the conventionally-managed with the peak in February and the minimum in different times depending on groves and years. AM colonization corresponded to a sigmoidal curve consisting of a laf phase during winter and a subsequent increase in spring, then succeeded by a maximum, and then a decrease at the end of vegetation. Fungal spore density and AM colonization showed a parallel pattern during the sample period. The seasonality appeared to be related more to the phenology of the plant than to the soil factors. Generally more spore density and AM colonization were found in organically managed groves. AM colonization was not correlated with available P and organic matter content in soil in this field investigation. Among sixteen weed species found in the groves, Astrogalus sinicus of Leguminosae, Portulaca oleracea of Portulacaceae showed high colonization in all groves and they can be considered as a source of inoculumn and host plants for propagation of AM fungi.
Kim Jong Duk;Kim Su Gon;Kwon Chan Ho;Abuel Sherwin J.;Chae Sang Heon;Kim Myoung Ki
Journal of The Korean Society of Grassland and Forage Science
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v.25
no.3
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pp.151-158
/
2005
This experiment was conducted to evaluate forage production and quality, and soil improvement of three legumes at Cheonan Yonam College. The three legumes used in the experiment were crimson clover (Trifolium incarnatum L.) red clover(Trifolium pratense L.) and chinese milk vetch(Astragalus sinicus L.). Flowering stage was observed on the 20th of April for chinese milk vetch and on the 30th of April for crimson clover, but the red clover did not produce flower until harvest time. The dry matter(DM) content of crimson clover was the highest among the three legumes. In terms of DM yield, crimson clover has the highest yield, the highest yield in crude protein(CP) and total digestible nutrients(TDN) was red clover. In terms of forage quality, the highest CP content was red clover, while the chinese milk vetch was the lowest for both neutral detergent fiber(NDF) and acid detergent fiberr(ADF). Red clove. and chinese milk vetch were highest fur TDN content, and the chinese milk vetch has the highest for relative feed value(RFV). Assessment of the chemical properties of soil after harvest showed that the nitrogen content of soil increased in all legumes. The organic matter(OM) content of soil in both crimson and red clover increased, while the chinese milk vetch decreased. The available $P_2O_5$ decreased in all three legumes, but the biggest decrease was in the chinese milk vetch. The exchangeable potassium in the soil for both crimson and red clover increased, but in chinese milk vetch it decreased. Based on the results of this study, the chinese milk vetch was superior in terms of forage quality, but crimson and red clover are excellent in forage yield and in maintaining soil quality in upland.
Forest soil fertility was evaluated through the approach of numerical method. In this study, the soil chemical properties analyzed for 35 different soil series as table 2 were cited in numerical analysis. Minimum contents of essential nutrients in the surface soil for a satisfactory growth of tree in the plantation were evaluated by comparing with Wild's standard as table 1. Demanding level of fertilization were evaluated by using the formula 1 as table 5. Similar relation of soil chemical properties between soil series were calculated through formula 2, and then classified into 5 groups in soil chemical properties. 1. General chemical properties of surface soil in case of 35 soil series. About 40 percent of 35 different soil series are less than 2 percent in organic matter, 10 ppm in available phosphorus, 1.25m.e/l00g in exchangeable calcium and 0.5m.e/l00g in exchangeable magnesium. Generally, shortage of exchangeable potash are not found. CEC less than 10m.e/l00g are in two thirds and strong acid soil less than PH 5.5 are in about four fifths. 2. Soil series requested or not the fertilization are indirectly evaluated from the formula 1 using the relative figure of chemical components of CEC, OM and MgO. Through this analysis, 8 different soil series have very poor quality in soil chemical capacity so that demands highly the fertilization. On the other hand, other 13 different soil series group have not been thought to need the fertilization according to chemical guality. 3. By the results comparing the similarity of chemical properties of forest soil, it is thought to be suitable that the forest soil fertility are divided into 5 groups as follows: 1. Low CEC soil 1-1 Low organic matter soil less than 2 percent 1-2 Medium organic matter soil less than 4 percent 2. High CEC and organic matter soil 2-1 Low magnesium soil 2-2 High magnesium soil 3. High magnesium and calcium soil as lime stone.
Kim, Ju-Hyeon;Kim, Mee-Jeong;Oh, Hyun-Kyung;Chang, Moon-Jeong;Kim, Sun-Hee
Journal of the East Asian Society of Dietary Life
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v.17
no.6
/
pp.860-875
/
2007
The purpose of this study was to investigate the seasonal variation in the mineral contents of fruits and vegetables available all the year -round. We analyzed Na, K, Mg, Ca, P, Fe Cr, Mn, Cu, Zn, Se, and Al concentrations by ICP-mass in 4 fruits and 17 vegetables items which are frequently consumed in Koreans. For Na content, fall apples showed the highest levels among 4 seasons, but fall tangerines contained the lowest amount of Na among the four seasons. Among the vegetables, the spinach, carrots, and lettuce contained relatively high amounts of Na. The K contents of cucumbers, cabbage, and zucchini were higher in the fall than in the other seasons. For Ca and Fe contents, spring strawberries, fall pears, and fall cabbage had the highest levels. The Cr contents of the apples were higher in the fall and winter than those were in the spring and summer. The strawberries contained their highest amounts of Cr, Mn, and Cu in the spring. The above results showed there are seasonal differences in the mineral contents of fruits and vegetables. Additionally, the fruits and vegetables tended to contain more minerals in the season they were harvested. Therefore, it is recommended to consume those fruits and vegetables during their harvest season.
One of the most common needle leaf species used in planting in Korea is korean pine (Pinus koraiensis S. et Z.), and jack pine (Pinus banksiana Lamb.) is one of the test species for suitability. The relation of nursery bed density of 2-1 korean pine and 1-1 jack pine was studied at the Kwang Nung Nursery, Central Branch Station of Forest Research Institute, and about 40km north of Seoul. Nursery bed density of 2-1 korean pine, which ranged from 36 to 324 trees per square meters and of 1-1 jack pine, which ranged from 25 to 169 trees per square meters, had a marked effect on caliper, height, dry weight and percent and amount of plantable stock. The soil physical and chemical properties is silt plus clay, 50.55 percent; organic matter, 2.09 percent; total nitrogen, 0.13 percent; available phosphorus, 253.25 ppm; exchangeable potash, 0.46 m.e/100g; and pH, 5.58. As the density of the nursery seedling stand of 2-1 korean pine increases, the average tree height increases (Fig. 1A), but in 1-1 jack pine density do not affect to increase or decrease the average tree height. As the density of nursery bed increases, the average stem caliper (at 2cm above ground line) and dry weight decrease (Fig. 1B), but the decreasing rate is more seriously in 1-1 jack pine than 2-1 korean pine (Fig.5). As increasing the density of nursery bed, the T/R ratio of trees of the test species increase. Also the dry weight of leaf, stem and root parts are decreasing in proportion to the increase of stand density, but the drop rate of jack pine is more rapid than korean pine (table. 1) The patent facts of difference of growth characteristics between 2-1 korean pine and 1-1 jack pine were studied. These facts should be used to select the scale of stand density at the nursery bed or the plantable site. Korean pine is demanded high density, on the other hand in jack pine low density are more suitable to manage the stand density. Stands of comparatively low density had the greatest percentage of high-quality stock, and the stands of high density had less than the high quality trees of low density. An important criterion of the best density is percent and number of high-quality trees produced per square meter of bed area. Stem caliper and stem height of seedling is used in most public nurseries to sort seedling into plantable grades. The stock grade standard has set at 4.5mm caliper and 16cm height of 2-1 korean pine as the minimum desired stem caliper and height. By the result studies, the plantable stock grade standards of 2-1 korean pine used at stem height 16cm and stem caliper 4.5mm from public nurseries should be reformed to stem height 18cm and stem caliper 4.0mm by the growth characteristics and the tree distribution of stem height and caliper of relation to density. For the 2-1 korean pine, best density should be about 160 to 200 trees per square meter according to soil fertility. For the 1-1 jack pine, the suitable standard of plantable stock should be at stem height 25cm and caliper 6mm (at 2cm above ground line) and best density was about 100 to 120 trees.
World production of mushrooms has been increasing 10-20% every year. Recently, Pleurotus eryngii and P. nebrodensis are very popular as new mushroom species for cultivation. Two kinds of mushrooms, Gumji (Ganoderma) and Soji, were described in old book of Samguksagi (History of the three kingdoms; 1145) in Koryo-dynasty. Many kinds of mushrooms were also described in more than 16 kinds of old books during Chosun-dynasty in Korea. One hundred and sixty commercial strains of 25 species in mushrooms were distributed to cultivators. By the way, only 8 varieties of them have registered variety protection. Mushroom industry as important export products developed from 1960 to 1980. Production of mushrooms as food was 181,828 metric tons valued at 800 billion Korean won in 2003. Isolated and identified substances from mushrooms are promising antifungal, antiinflammatory, antitumor, antiviral (anti-HIV), antibacterial & antiparasitic, antidiabetic, immunomodulating, kidney tonic, hepatoprotective, nerve tonic, and sexual potentiator. These substances can also be used for blood pressure regulation and effective against cardiovascular disorders, hypocholesterolemia & hyperlipidemia, and chronicbronchitis. Mushroom products including pharmaceuticals, tonics, healthy beverages, functional biotransformants, and processed foods have also became available on the markets. Compost and feed can likewise be made from mushroom substrates after harvest. The mushroom industry is already one of the fastest growing investment sectors in Korea. By the way, there is a need to strain improvement for variety protection, advanced cultivation technology at low cost for growers, and control of demand and supply for marketing in order to more upgrade development of mushroom industry in the future.
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