• Molecules and Cells
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• 제27권3호
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• pp.359-363
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• 2009

Chfr, a checkpoint with FHA and RING finger domains, plays an important role in cell cycle progression and tumor suppression. Chfr possesses the E3 ubiquitin ligase activity and stimulates the formation of polyubiquitin chains by Ub-conjugating enzymes, and induces the proteasome-dependent degradation of a number of cellular proteins, including Plk1 and Aurora A. While Chfr is a nuclear protein that functions within the cell nucleus, how Chfr is localized in the nucleus has not been clearly demonstrated. Here, we show that nuclear localization of Chfr is mediated by nuclear localization signal (NLS) sequences. To reveal the signal sequences responsible for nuclear localization, a short lysine-rich stretch (KKK) at amino acid residues 257-259 was replaced with alanine, which completely abolished nuclear localization. Moreover, we show that nuclear localization of Chfr is essential for its checkpoint function but not for its stability. Thus, our results suggest that NLS-mediated nuclear localization of Chfr leads to its accumulation within the nucleus, which may be important in the regulation of Chfr activation and Chfr-mediated cellular processes, including cell cycle progression and tumor suppression.

• 대한음성학회:학술대회논문집
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• 대한음성학회 2006년도 춘계 학술대회 발표논문집
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• pp.37-40
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• 2006

In this paper, we propose a VQ codebook design of speech recognition feature parameters in order to improve the performance of a distributed speech recognition system. For the context-dependent HMMs, a VQ codebook should be correlated with phonetic distributions in the training data for HMMs. Thus, we focus on a selection method of training data based on phonetic distribution instead of using all the training data for an efficient VQ codebook design. From the speech recognition experiments using the Aurora 4 database, the distributed speech recognition system employing a VQ codebook designed by the proposed method reduced the word error rate (WER) by 10% when compared with that using a VQ codebook trained with the whole training data.

• 음성과학
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• 제15권2호
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• pp.55-65
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• 2008

Recently, the multi-model based speech recognizer has been used quite successfully for noisy speech recognition. For the selection of the reference HMM (hidden Markov model) which best matches the noise type and SNR (signal to noise ratio) of the input testing speech, the estimation of the SNR value using the VAD (voice activity detection) algorithm and the classification of the noise type based on the GMM (Gaussian mixture model) have been done separately in the multi-model framework. As the SNR estimation process is vulnerable to errors, we propose an efficient method which can classify simultaneously the SNR values and noise types. The KL (Kullback-Leibler) distance between the single Gaussian distributions for the noise signal during the training and testing is utilized for the classification. The recognition experiments have been done on the Aurora 2 database showing the usefulness of the model compensation method in the multi-model based speech recognizer. We could also see that further performance improvement was achievable by combining the probability density function of the MCT (multi-condition training) with that of the reference HMM compensated by the D-JA (data-driven Jacobian adaptation) in the multi-model based speech recognizer.

• BMB Reports
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• 제44권3호
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• pp.147-157
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• 2011

The meiotic process from the primordial stage to zygote in female germ cells is mainly adjusted by post-transcriptional regulation of pre-existing maternal mRNA and post-translational modification of proteins. Several key proteins such as the cell cycle regulator, Cdk1/cyclin B, are post-translationally modified for precise control of meiotic progression. The second messenger (cAMP), kinases (PKA, Akt, MAPK, Aurora A, CaMK II, etc), phosphatases (Cdc25, Cdc14), and other proteins (G-protein coupled receptor, phosphodiesterase) are directly or indirectly involved in this process. Many proteins, such as CPEB, maskin, eIF4E, eIF4G, 4E-BP, and 4E-T, post-transcriptionally regulate mRNA via binding to the cap structure at the 5' end of mRNA or its 3' untranslated region (UTR) to generate a closed-loop structure. The 3' UTR of the transcript is also implicated in post-transcriptional regulation through an association with proteins such as CPEB, CPSF, GLD-2, PARN, and Dazl to modulate poly(A) tail length. RNA interfering is a new regulatory mechanism of the amount of mRNA in the mouse oocyte. This review summarizes information about post-transcriptional and post-translational regulation during mouse oocyte meiotic maturation.

• BMB Reports
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• 제49권10호
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• pp.527-528
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• 2016

In embryonic stem cells (ESCs), cell cycle regulation is deeply connected to pluripotency. Especially, core transcription factors (CTFs) which are essential to maintaining the pluripotency transcription programs should be reset during M/G1 transition. However, it remains unknown about how CTFs are governed during cell cycle progression. Here, we describe that the regulation of Oct4 by Aurora kinase b (Aurkb)/protein phosphatase 1 (PP1) axis during the cell cycle is important for resetting Oct4 to pluripotency and cell cycle related target genes in determining the identity of ESCs. Aurkb starts to phosphorylate Oct4(S229) at the onset of G2/M phase, inducing the dissociation of Oct4 from chromatin, whereas PP1 binds Oct4 and dephosphorylates Oct4(S229) during M/G1 transition, which resets Oct4-driven transcription for pluripotency and the cell cycle. Furthermore, Aurkb phosphormimetic and PP1 binding-deficient mutations in Oct4 disrupt the pluripotent cell cycle, lead to the loss of pluripotency in ESCs, and decrease the efficiency of somatic cell reprogramming. Based on our findings, we suggest that the cell cycle is directly linked to pluripotency programs in ESCs.

• 한국발생생물학회지:발생과생식
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• 제18권1호
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• pp.65-71
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• 2014

Cell cycle process is regulated by a number of protein kinases and among them, serine/threonine kinases carry phosphate group from ATP to substrates. The most important three kinase families are Cyclin-dependent kinase (Cdk), Polo-like kinase (Plk), and Aurora kinase. Polo-like kinase family consists of 5 members (Plk1-Plk5) and they are involved in multiple functions in eukaryotic cell division. It regulates a variety of aspects such as, centrosome maturation, checkpoint recovery, spindle assembly, cytokinesis, apoptosis and many other features. Recently, it has been reported that Plks are related to tumor development and over-expressed in many kinds of tumor cells. When injected the anti-Plk antibody into human cells, the cells show aneuploidy, and if inhibit Plks, most of the mitotic cell division does not proceed properly. For that reasons, many inhibitors of Plk have been recently emerged as new target for remedy of the cancer therapeutic research. In this paper, we reviewed briefly the characteristics of Plk families and how Plks work in regulating cell cycles and cancer formation, and the possibilities of Plks as target for cancer therapy.

• Journal of Astronomy and Space Sciences
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• 제21권2호
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• pp.109-120
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• 2004

2003년 9월 발사된 과학기술위성 1호의 탑재체 원자외선 분광기(FIMS)기는 우주 및 오로라 관측을 주 임무로 하고있다. 본 논문에서는 원자외선 분광기의 성공적인 천문 관측을 지원하기 위해, 탑재 컴퓨터와 원자외선 분광기와의 간단하고 신뢰성 있는 시간 동기화 기법을 소개한다. 이것은 과학기술위성 1호라는 특별한 환경 하에서 위성의 신뢰성을 줄이지 않으면서 시간 동기화를 이루도록 고안된 방법이다. 정확도가 서로 다른 두 가지 방법이 제시되었으며, 주된 방법을 사용할 수 없을 때 정확도가 낮은 방법을 사용함으로써 고장에 대처할 수 있도록 하였다. 이 기법들은 위성 시스템의 변경을 최소화하고 간단한 회로를 사용함으로써 구현에 따른 비용을 절감하고 고도의 신뢰성을 유지하도록 설계되었다.

• 천문학회보
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• 제36권2호
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• pp.101.2-101.2
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• 2011

We have developed solar and space weather monitoring system for space weather users since 2007 as a project named 'Construction of Korea Space Weather Prediction Center'. In this presentation we will introduce space weather monitoring system for Geostationary Satellites and Polar Routes. These were developed for satisfying demands of space weather user groups. 'Space Weather Monitoring System for Geostationary Satellites' displays integrated space weather information on geostationary orbit such as magnetopause location, nowcast and forecast of space weather, cosmic ray count rate, number of meteors and x-ray solar flux. This system is developed for space weather customers who are managing satellite systems or using satellite information. In addition, this system provides space weather warning by SMS in which short message is delivered to users' cell phones when space weather parameters reach a critical value. 'Space Weather Monitoring System for Polar Routes' was developed for the commercial airline companies operating polar routes. This provides D-region and polar cap absorption map, aurora and radiation particle distribution, nowcast and forecast of space weather, proton flux, Kp index and so on.

• 한국전문물리치료학회지
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• 제7권4호
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• pp.16-38
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• 2000

일상생활동작(activities of daily living)을 주요 목적이자 치료 수단으로 사용하고 있는 작업치료사들이 그 활동 중의 하나인 성적 욕구의 표현(sexual expression)에 대해 체계적인 접근이 미진하다는 지적을 극복 하고자 본 연구가 계획되었다. 특히 만성 장애가 있는 65세 이상 연령층의 성생활에 대해 기술적으로 접근하는 방법을 고안하는 것은 상당한 임상적 의미를 부여 할 수 있다. 본 연구의 주된 목적은 관절염이 65세 이상의 미국인의 성생활에 미치는 영향과 대책에 대한 논의를 작업치료사의 입장에서 자연스럽게 유도하기 위한 질문내용을 고안하는데 있다. 본 연구자들은 광범위한 문헌연구를 바탕으로 7개항의 질문을 작성하여 10명의 노인학 전공 작업치료사들에게 비평을 의뢰하였다. 7명의 작업치료사들이 전문가로서의 세밀한 답변을 해주었고 그들은 공통적으로 일상생활 중의 하나인 성생활이 삶의 질을 향상시키는데 매우 중요한 역할을 함을 강조하였다. 이들 전문가들의 조언을 바탕으로 새로운 질문서가 완성되었다.

• Molecules and Cells
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• 제41권5호
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• pp.444-453
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• 2018

Aberrations in histone modifications are being studied in mixed-lineage leukemia (MLL)-AF9-driven acute myeloid leukemia (AML). In this study, we focused on the regulation of the differentiation of the MLL-AF9 type AML cell line THP-1. We observed that, upon phorbol 12-myristate 13-acetate (PMA) treatment, THP-1 cells differentiated into monocytes by down-regulating Aurora kinase A (AURKA), resulting in a reduction in H3S10 phosphorylation. We revealed that the AURKA inhibitor alisertib accelerates the expression of the H3K27 demethylase KDM6B, thereby dissociating AURKA and YY1 from the KDM6B promoter region. Using Flow cytometry, we found that alisertib induces THP-1 differentiation into monocytes. Furthermore, we found that treatment with the KDM6B inhibitor GSK-J4 perturbed the PMA-mediated differentiation of THP-1 cells. Thus, we discovered the mechanism of AURKA-KDM6B signaling that controls the differentiation of THP-1 cells, which has implications for biotherapy for leukemia.