• Title/Summary/Keyword: attached growth

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Growth of Green Pepper(Capsicum annuum L.) in a Plastic Greenhouse Covered with Anti-dropping Plasma Film (방적성 Plasma 처리 필름으로 피복된 플라스틱온실의 풋고추 생육)

  • Chun, Hee;Kim, Kyung-Je;Kim, Jin-Young;Kim, Hyun-Hwan;Lee, Si-Young
    • Journal of Bio-Environment Control
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    • v.9 no.3
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    • pp.156-160
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    • 2000
  • The Plasma film treated with a high electric voltage was developed to enhance flow down of condensation drops on inside plastic film. Arch type greenhouse framed with iron pipe of 25mm diameter defand 1.5mm thickness were covered with either the developed plasma film or surfactant film(control). Green pepper seedlings raised for 40 days in plug trays were transplanted at a density of 110cm by 30cm in each greenhouse. The mount of condensational water on film surface, generated by 7$0^{\circ}C$ water bath chimney systems and flew down, was collected and measured. The amount of collected water after 150 minutes was 2.56 mL.100c $m^{-2}$ and 0.94mL.100c $m^{-2}$ , respectively, in the plasma film and surfactant film-covered greenhouses. The amount of condensational water drops attached on the cover at 08:20 a.m. at 60 days filter covering was 0.34mL.100c $m^{02}$ and 0.32mL.100c $m^{-2}$ , respectively, in the plasma film- and surfactant film-covered greenhouses. Solar irradiance transmitted into greenhouse was 2.0% higher in the greenhouse covered with the plasma film tan that in the greenhouse covered with the surfactant film. Air temperature in the plasma film-covered greenhouse was higher than the surfactant film-covered greenhouse by 0.5$^{\circ}C$. However, there was no difference in relative humidity between the two greenhouse. Plant height, leaf area, dry weight and early yield showed no significant differences.s.

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Effects of PLGA/Fibrin Scaffolds on Attachment and Proliferation of Costal Cartilage Cells (PLGA/피브린 지지체가 늑연골 세포의 부착과 성장에 미치는 영향)

  • Song, Jeong Eun;Lee, Yujung;Lee, Yun Me;Cho, Sun Ah;Jang, Ji Eun;Lee, Dongwon;Khang, Gilson
    • Polymer(Korea)
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    • v.37 no.2
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    • pp.141-147
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    • 2013
  • Poly(lactide-co-glycolic acid) (PLGA) has been widely used in the drug delivery and tissue engineering applications because of its good mechanical strength and biodegradation profile. However, cell attachment to the scaffold is low compared with that on fibrin although cells can be attached to the polymer surface. In this study, PLGA scaffolds were soaked in cells-fibrin suspension and polymerized with dropping fibrinogen-thrombin solution. Cellular proliferation activity was observed in PLGA/fibrin-seeded costal cartilage cells (CC) on 1, 3, and 7 days using the MTT assay and SEM. The effects of fibrin on the extracellular matrix (ECM) formation were evaluated using CC cell-seeded PLGA/fibrin scaffolds. The PLGA/fibrin scaffolds elicited more production of glycosaminoglycan (GAG) and collagen than the PLGA scaffold. In this study, fibrin incorporated PLGA scaffolds were prepared to evaluate the effects of fibrin on the cell attachment and proliferation in vitro and in vivo. In this result, we confirmed that proliferation of cells in PLGA/fibrin scaffolds were better than in PLGA scaffolds. The PLGA/fibrin scaffolds provide suitable environment for growth and proliferation of costal cartilage cells.

In-Vitro Thrombosis Detection of Mechanical Valve using Artificial Neural Network (인공신경망을 이용한 기계식 판막의 생체외 모의 혈전현상 검출)

  • 이혁수;이상훈
    • Journal of Biomedical Engineering Research
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    • v.18 no.4
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    • pp.429-438
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    • 1997
  • Mechanical valve is one of the most widely used implantable artificial organs of which the reliability is so important that its failure means the death of patient. Therefore early noninvasive detection is essentially required, though mechanical valve failure with thrombosis is the most common. The objective of this paper is to detect the thrombosis formation by spectral analysis and neural network. Using microphone and amplifier, we measured the sound from the mechanical valve which is attached to the pneumatic ventricular assist device. The sound was sampled by A/D converter(DaqBook 100) and the periodogram is the main algorithm for obtaining spectrum. We made the thrombosis models using pellethane and silicon and they are thrombosis model on the valvular disk, around the sewing ring and fibrous tissue growth across the orifice of valve. The performance of the measurment system was tested firstly using 1 KHz sinusoidal wave. The measurement system detected well 1KHz spectrum as expected. The spectrum of normal and 5 kinds of thrombotic valve were obtained and primary and secondary peak appeared in each spectrum waveform. We find that the secondary peak changes according to the thrombosis model. So to distinguish the secondary peak of normal and thrombotic valve quantatively, 3 layer back propagation neural network, which contains 7, 000 input node, 20 hidden layer and 1 output was employed The trained neural network can distinguish normal and valve with more than 90% probability. As a conclusion, the noninvasive monitoring of implanted mechanical valve is possible by analysing the acoustical spectrum using neural network algorithm and this method will be applied to the performance evaluation of other implantable artificial organs.

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Identification and Characterization of Entomopathogenic Fungi Isolated from Bemisia tabaci in Korea (담배가루이(Bemisia tabaci )에서 분리한 곤충병원성진균의 동정 및 병원성 검정)

  • Park, Hyun-Rho;Ryu, Young-Hyun;Yeon, Il-Kyen;Nam, Sung-Hee;Kim, Dong-Geun;Han, Myung-Sae
    • Korean journal of applied entomology
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    • v.53 no.1
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    • pp.27-34
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    • 2014
  • Entomopathogenic fungi were isolated from Bemisia tabaci in an Oriental melon field, and their growth characteristics, factors related to a natural outbreak, and infectivity against Bemisia tabaci, Tetranychus urticae, and Frankliniella intonsa were investigated. The isolates had erect conidiophores bearing whorls of 4-6 phialides with a swollen base where cylindrical conidia of $3.0-3.4{\mu}m$ were attached. The isolates were identified as Isaria fumosorosea on the basis of morphological characteristics and an ITS sequence with 99% similarity. I. fumosorosea IFs-08 grew well on Sabouraud dextrose agar+yeast extract medium(3.2 mm/day/$24^{\circ}C$); it grew better at $35^{\circ}C$ than at $15^{\circ}C$. The isolates of I. fumosorosea-IFs were highly infective and killed 93.9-96.7% B. tabaci, 84.9-92.0% T. urticae, and 81.5-84.4% F. intonsa in bioassay, whereas three isolates (Isaria tenuipes, Isaria farinosa, and Isaria fumosorosea) from KACC showed a low infectivity of 10-20%. To the best of our knowledge, this is the first report of I. fumosorosea isolated from B. tabaci in Korea.

Dedifferentiated Parosteal Osteosarcoma - A Case Report - (역분화성 방골성 골육종 - 증례 보고 -)

  • Sung, Ki-Sun;Chang, Moon-Jong;Lim, Kyung-Sub
    • The Journal of the Korean bone and joint tumor society
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    • v.13 no.2
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    • pp.195-200
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    • 2007
  • Dedifferentiated parosteal osteosarcoma is an uncommon variant of osteosarcoma. Dedifferentiation is known to be associated with a greater metastatic potential and a more rapid lethal clinical course. Thus recognition of dedifferentiation is important to establish the treatment strategy. But there may be few significant clinical clues to distinguish between dedifferentiated parosteal osteosarcoma and conventional one. A 29-year-old woman presented with 2-year history of discomfort and swelling in her proximal thigh. Examination showed a large, hard, non-mobile mass. Radiographs revealed a large ossified mass attached to the proximal femur. Diagnosis of parosteal osteosarcoma was established by MRI and needle biopsy. But she had a history of abrupt severe thigh pain and increased swelling before surgery. Follow up MRI showed enlargement of mass with invasion to muscle around tumor. The patient underwent an en-bloc resection of tumor and reconstruction. Histological examination showed parosteal sarcoma with dedifferentiation. The patient expired due to local recurrence of tumor and distant lung metastasis 2 months after the surgery. In case with rapid growth of a lesion or unusual severe pain, one must have a high index of suspicion with regard to dedifferentiation.

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The Cadmium Biosorption Mechanism in Gram Negative Bacteria, Serratia marcescens (Gram 음성 세균인 Serratia marcescens에 의한 카드뮴 흡착 기작)

  • 이호용;민봉희;최영길
    • The Korean Journal of Ecology
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    • v.22 no.1
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    • pp.39-43
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    • 1999
  • Serratia marcescens, an enterobacterium of gram-negative bacteria, is characterized by resistance of the admium. Cadmium sensitive PM strain did not grow in the medium at cadmium concentration of 50 ppm. PA strain was induced to accommodate to cadmium by cultivating the mother strain (PC strain) in the medium with 50 ppm cadmium. As compared with PC and PM strains, PA strain revealed the excellent growth in cadmium media and accumulated four to five times higher cadmium concentration in cell than other strains. PA strain accumulated 23% of cadmium in cells when cultured in medium treated with 100 ppm cadmium and this cadmium was more accumulated in cytosol fractions than membrane fractions. Analysis by TEM indicated that cadmium was concentrated as a form of granule in cytosol. In protein patterns of cell after the treatment of cadmium, two inducible proteins (28 KDa and 64 KDa) and one reducible protein (45 KDa) were detected by SDS-PAGE. By Atomic Absorption Spectrophotometer, the amounts of cadmium attached to inducible proteins of 28 KDa and 64 KDa were 318.28 ㎍ and 325.37 ㎍ per gram of protein, respectively. It is assumed that these inducible proteins play an important role in the mechanism of cadmium accumulation in cells. A plasmid of 23Kbp was found in S. marcescens. The ability of resistance to cadmium in plasmid was confirmed by curing experiments.

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TREATMENT OF OKC ON RAMUS OF MANDIBLE BY SAGITTAL SPLITTING TECHNIQUE (하악 상행지부에 발생한 치성각화낭의 시상 분할 골절단술을 이용한 치험례)

  • Song, Hyun-Woo;Ryu, Dong-Mok;Kim, Yeo-Gab;Lee, Baek-Soo;Kwon, Young-Dae;Choi, Byung-Jun;Kim, Young-Ran;Yim, Jin-Hyuk;Lee, Jung-Gyo
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.31 no.5
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    • pp.408-413
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    • 2009
  • Odontogenic keratocyst (OKC) is a epithelial developmental cyst which were first described by Phillipsen in 1956. The frequency of OKC has been reported to vary from 3% to 11% of odontogenic cysts. The most characteristic clinical aspect of OKC is the high frequency of recurrence. The mechanism of recurrence is thought to be related to residues of cyst epithelium and an intrinsic growth potential following excision. And since the lining of the OKC is thin and friable, removal of the cyst in one piece may sometimes be difficult. Complete removal of the cyst lining without leaving behind remnants attached to the soft tissue or bone is necessary to avoid recurrence. Therapeutic approaches vary in different studies from marsupialization and enucleation, which may be combined with adjuvant therapy such as cryotherapy or Carnoy's solution, to marginal or radical resection. The recurrent rate varies from approximately 20% to 62%. And OKC in the angle-ramus region of the mandible had a higher tendency to recur, because of the difficulty in accessing and removing OKC from the ramus. By employing a sagittal splitting of the mandible a good surgical access was provided and cyst could be removed completely. We present an illustrative case of a small, lobulated OKC that involved ramus on mandible, and a review of the contemporary literature.

Biological Nitrification and Denitrification for Landfill Leachate Containing High Concentration of Ammonium-Nitrogen by using MLE Process (MLE 공정을 이용한 고농도 NH4+-N 함유 침출수의 생물학적 질산화/탈질)

  • Won, Jong-Choul;Namkoong, Wan;Bae, Young-Shin;Lee, Kyung-Shin;Park, Ki-Hyuk;Song, Su-Sung;Yoon, Cho-Hee
    • Journal of Korean Society of Environmental Engineers
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    • v.22 no.6
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    • pp.1027-1035
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    • 2000
  • This study was carried out to investigate the treatability of landfill leachate having high concentration of ammonium nitrogen with/without the circulation of media in pilot-scale($48m^3basis$) process. Total nitrogen removal efficiency was relatively increased in the media added process (influent ; $1.230{\sim}2,000mg{\cdot}l^{-1}$, effluent ; $120{\sim}250mg{\cdot}l^{-1}$) compared with the control process. The difference of nitrogen removal efficiency between these processes may be due to that stable growth of nitrifiers attached to the media could be achieved 99.3% of ammonium-nitrogen removal efficiency(without ; 98.2%) and 88.5% of total nitrogen removal efficiency(without ; 85.8%) were shown in media added process, respectively. Also, optimum BOD/ $NH_4{^+}$-N ratio was relatively decreased in the media process compared with the control process. Sludge settleability, on the other hand. was shown better in media added process than in control process. This outstanding sludge settleability in the media added process indicates the compatibility of media(zeolite) to the microorganism and the possibility of using media of biofilm process.

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Effects of Fire Curtain and Forced Smoke Ventilation on Smoke Spread to Auditorium in Stage Fire of Theater (공연장 무대 화재 시 방화막과 강제 배연구가 객석으로의 연기 확산에 미치는 영향)

  • Kim, Jae Han;Kim, Duncan;Lee, Chi Young
    • Fire Science and Engineering
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    • v.31 no.5
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    • pp.28-36
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    • 2017
  • In this work, the effects of fire curtain and forced smoke ventilation on smoke spread to auditorium in the stage fire of theater were investigated using the Fire Dynamics Simulator (FDS). For the stage of 31 m (Width)${\times}$34 m (Depth)${\times}$32 m (Height) in dimension, the fast growth fire condition with 10 MW of heat release rate was applied. The forced smoke ventilation was set based on the National Fire Safety Code (NFSC) and previous research. The gap distances between the fire curtain and proscenium wall was established to be 0 m and 0.5 m. When the fire curtain was attached completely to the proscenium wall without any gap, no smoke spread from the stage to the auditorium occurred, independent of forced smoke ventilation. When the gap distance between the fire curtain and proscenium wall was 0.5 m, the smoke layer in the stage descended to the lower height from the bottom than the case without the fire curtain, which was because the smoke spread to auditorium was impeded by the fire curtain. Under the same fire curtain condition, the case with the forced smoke ventilation led to decreasing the mass flow rate of outflow through the gap between the fire curtain and proscenium wall, as compared to the case without the forced smoke ventilation. Based on this study, it was confirmed that the fire curtain and forced smoke ventilation were the effective tools to hold down the smoke spread to the auditorium in the stage fire of theater.

Effect of Wild Plant Extracts Based on Korea Traditional Prescription on Endurance Exercise Performance during Calorie Restriction Period (초저열량식이로 인한 체력 저하에서 산야초추출물의 지구력 증진 효과)

  • Kim, Joong-Hark;Kim, Hwa-Young;Chang, Hey-Eun;Chung, Ji-Sang;Hwang, Sung-Joo;Park, Mi-Hyoun;Hong, Seong-Gil
    • Korean Journal of Food Science and Technology
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    • v.38 no.1
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    • pp.147-151
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    • 2006
  • Effects of wild plant extract (Lak) based on Korea traditional prescription on maximal exercise performance and endurance were evaluated using calorie-restriction animal model. In acute forced swimming test with 10% body weight attached to tail, dietary Lak supplementation increased exercise performance endurance by increasing concentrations of ATP and insulin-like growth factor-1 (IGF-1) under calorie-restriction condition, and decrement of blood lactic acid concentration and increment of muscle ATP content were observed. These results suggest Lak is very effective for decreasing side-effects of obesity therapy using very low calorie diet.