• BMB Reports
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• v.48 no.6
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• pp.354-359
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• 2015

Vascular smooth muscle cells (VSMCs) undergo death during atherosclerosis, a widespread cardiovascular disease. Recent studies suggest that oxidative damage occurs in VSMCs and induces atherosclerosis. Here, we analyzed oxidative damage repair in VSMCs and found that VSMCs are hypersensitive to oxidative damage. Further analysis showed that oxidative damage repair in VSMCs is suppressed by a low level of poly (ADP-ribosyl)ation (PARylation), a key post-translational modification in oxidative damage repair. The low level of PARylation is not caused by the lack of PARP-1, the major poly(ADP-ribose) polymerase activated by oxidative damage. Instead, the expression of poly(ADP-ribose) glycohydrolase, PARG, the enzyme hydrolyzing poly(ADP-ribose), is significantly higher in VSMCs than that in the control cells. Using PARG inhibitor to suppress PARG activity facilitates oxidative damage-induced PARylation as well as DNA damage repair. Thus, our study demonstrates a novel molecular mechanism for oxidative damage-induced VSMCs death. This study also identifies the use of PARG inhibitors as a potential treatment for atherosclerosis. [BMB Reports 2015; 48(6): 354-359]

• Journal of the Korean Society of Costume
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• v.28
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• pp.137-150
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• 1996

The objectives of this study were to classify daily clothing selection motives and to group men into life style types and to examine the differences in daily clothing selection motives according to the life style types. Questionnaire comprised of three sections: 36 Likert type items of life style measure; 29 Likert type items of daily clothing selection motives measure: and 4 demographic variables. Samples were 267 salary men in gheir 20's to 30's in Seoul Korea. The data were analyzed using factor analy-sis cluster analysis one-way ANOVA Dun-can's multiple range test and t-test. 1. Five factors of life style were derived by factor analysis ; F. 1 'appearance oriented' f. 2. 'positive activity' F. 3 'economy oriented': F.4 'active-leisure';F. 5 'contemporary' Four types of life style of men were defined by cluster analysis of the five factors: T. 1'posi-tive activity'; T. 2.'economy interest'; T. 3'contemporary' : T 2'economy interest' T.3'contemporary' ; T. 4 'appearance interest' 2. Four factors of daily clothing selection motives were derived by factor analysis: F.1 'clothing harmony'; F.2 'clothing utility'; F.3 'psychological influence'; F.4 'exogenous situ-ation adaptation 3. There were significant differences in daily clothing selection motives according to the life style types. The types of positive activity economy interest and contemporary had 'clothing utility' motive more than appearance interest type. Economy interest type had 'hpsychological influence' motive more than other three types. Economy interest type and appearance interest type had 'exogenous situ-ation adaptation' motive more than other two types 4. 20's and unmarried men had'psychological influence' motive more than 30's and married. Upper class had 'clothing harmony' and 'ex-ogeneous situation adaptation' motives more than middle and low. Men with less education had ' clothing utility' and 'exogenous situation adaptation' motives more than did more edu-cation.

• Journal of the Korean Chemical Society
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• v.45 no.6
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• pp.549-554
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• 2001

For the development of new COX-2 inhibitor, novel compound were synthesized through induction an arylsulfonyl group to 1-position, an arylcarboxamidyl group to 2-position and an alkyl group to 4-position of L-proline. We started from 4-hydroxy-L-proline, the 4-methylphenylsulfonyl of 1-position was introduced through N-tosylation and the carboxylic acid group was protected by esterification. We sucessfully converted to a various derivatives 4b-d for O-alkyl-(or aralkyl)ation of 4-position using silver oxide as catalysis. The 4-alkyloxy-1-tosyl L-prolines 5b-d were synthesized through base-hydrolysis for the deprotection of carboxylic acid. Final compound 1,2,4-substituted pyrrolidines, 4-alkyloxy-2-phenylcarboxamidyl-1-tosyl pyrrolidines 6a-d were synthesized through the condensation of arylamine with 3 and 5b-d using DCC.

• YAKHAK HOEJI
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• v.45 no.6
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• pp.650-655
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• 2001

Carvedilol is a nonselective $\beta$-blocking agent with vasodilating properties that are attributed mainly to its blocking activity at $\alpha$$^{1}$-receptors. Carvedilol is used in the treatment of mild to moderate hypertention and angina pectoris and is often used in combination with other drugs. This study was carried out to evaluate the bioequivalence and pharmacokinetics of two carvedilol 25mg tablet formulations according to the guidelines of Korea Food and Drug Administration (KFDA). Twenty healthy volunteers are enrolled and received a single dose (25mg as carvedilol) of each drug in the fasting state, in a randomized 2-way crossover design. After oral administration, blond samples were collected for a period of 30 hours. Plasma concentrations of carvedilol were determined by a rapid and sensitive HPLC method with spectrofluorometric detection. The major pharmacokinetic parameters such as AU $C_{0-}$30hr/, AU $C_{inf}$ , $C_{max}$, $T_{max}$, $t_{1}$2 / Cl/F and V $_{\beta}$//F were calculated. ANOVA test and t-test were utilized for the statistical analysis of each parameter. The results showed that the differences in AU $C_{0-}$30hr/, $C_{max}$ and $T_{max}$ between two were ~5.66, 1.74 and 0.00%, respectively. Minimum detectable differences ($\Delta$) at $\alpha$=0.05 were less than$\pm$ 20% except $T_{max}$ (8.44, 18.36, and 33.86%, respectively). The 90% confidence intervals of all parameters were within $\pm$20% (-10.60~ -0.72, -9.00~12.49 and -19.81~19.81%, respectively). Therefore, it is concluded that the two formulations are bioequivalent for both the extent and the rate of absorption after single dose administration.ation.ion.ion.ation.ion.n.

• Membrane Journal
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• v.6 no.1
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• pp.44-52
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• 1996

For the purpose of the optimal design of a frame and plate type of pervaporation module, model equations which can predict the effects of feed flow condition on the temperature distribution of the feed developed in the module were established and the temperature distribution with feed flow condition was investigated through the model si$$\mu$ation. With increasing the Reynolds number of feed flow in the module, the flow velocity gradient in the channel height-direction as well as the volume rate of feed which acts as energy source for the evaporation of perrneants on the permeate-side surface of a membrane increased to such an extent that both mass and heat flux in the channel height direction could increase and the temperature drop of feed due to the evaporation of the permeant could be reduced correspondingly. A decrease in channel height caused the temperature drop of feed because of decreasing feed flow in the module. It was observed that the si$$\mu$ation result on the effect of Re on the temperature distribution of feed in the module has an agreement with experiment.

• Biotechnology and Bioprocess Engineering:BBE
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• v.8 no.2
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• pp.101-105
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• 2003

Recombinant bioluminescent bacteria were used to monitor and classify the to xicity of azo dyes. Two constitutive bioluminescent bacteria, Photobacterium phosphoreum and Es-Cherichia coli, E, coli GC2 (lac::luxCOABE), were used to detect the cellular toxicity of the azo dyes. In addition, four stress-inducible bioluminestent E. coli, DPD2794 (recA::luxCDABE), a DNA damage Sensitive strain; DPD2540 (fabA::luxCDABE), a membrane damage sensitive strain; DPD2511 (katG::luxCDABE), an oxidative damage sensitive strain; and TV1061 (grpE::luxCDABE), a protein damage sensitive strain, were used to provide information about the type of toxicity caused by crystal violet, the most toxic dye of the 16 azo dyes tested. These results suggest that azo dyes result in serious cellular toxicity in bacteria, and that toxicity monitoring and classific ation of some azo dyes, In the field, may be possible using these recombinant bioluminescent bacteria.

• Journal of Korean Institute of Industrial Engineers
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• v.33 no.1
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• pp.44-51
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• 2007

The purpose of this study was to identify the relationship between the cumulative fatigue of trunk muscles andthe period of recovery time during repetitive lifting and lowering tasks with symmetric and asymmetric postures.Ten subjects participated in the experiment. Subjects had 1, 2, 3, 4 and 5 minutes recovery time respectivelywhile they were performing the lifting and lowering task repeatedly for 3 minutes with the weight equivalent to25% level of MVC. EMG signals from ten trunk muscles were collected and the fatigue level was analyzedquantitatively. In results, the local muscle fatigue was no longer accumulated when 5 minutes recovery time wasgiven in symmetric position. For asymmetric position, it took longer minutes to prevent the fatigue accumul-ation. Different trunk muscles indicated slightly different recovery patterns in terms of MPF (Mean Powerfrequency) value.This result would help ergonomist design the length of recovery time to control the cumulative fatigue of trunkmuscles in industry with repetitive lifting and lowering task.

• 한국기계연구소 소보
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• s.9
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• pp.141-151
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• 1982

An iterative procedure for the calculation of the thick axisymmetric boundary layer and wake near the stern of a body of revolution is presented. Procedure consists of the potential flow calculation by a method of the integral equation of first kind and the calculation of boundary layer and wake by a differential me¬thod of the boundary layer theory. Additionally, higher order terms are included in the conventional momentum equations and continuity equation for the consider¬ation of the characteristics of axisymmetric flow different from the one of two dimentional flow and the thick boundary layer. These solutions are matched at the edge of boundary layer and wake. The results obtained by the present me¬thod are compared with the experimental data and it is found that the nominal wake distribution at the propeller plane of a axisymmetric body is in good agree¬ment with the experiment.

• Journal of Agricultural Extension & Community Development
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• v.2 no.2
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• pp.135-145
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• 1995

The objectives of this study were 1) to investigate the important items affecting the farmers' education system, 2) to estimate their influences on the farmers' education and 3) to present the development directions of farmers education under the changing circumstances of localization. The major or results and implications were as follows. 1. The important items of farmers education were authority in organizing the agricultural extension agency, personnel management, finance, and inspection and audit. 2. The development directions of farmers education were such views as differenti-ation of educational functions of various levels extension organizations, extensionists' competence, developing programs corresponding with farmers needs, participation of farmers, and expanding the educational opportunity for urban-ites and the old generation, etc.

• Korean Journal of Hydrosciences
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• v.6
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• pp.51-66
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• 1995

Various Eulerian-Lagerangian numerical models for the one-dimensional longtudinal dispersion equation are studied comparatively. In the models studied, the transport equation is decoupled into two component parts by the operator-splitting approach ; one part governing advection and the other dispersion. The advection equation has been solved using the method of characteristics following flud particles along the characteristic line and the result are interpolated onto an Eulerian grid on which the dispersion equation is solved by Crank-Nicholson type finite difference method. In solving the advection equation, various interpolation schemes are tested. Among those, Hermite interpo;ation po;ynomials are superor to Lagrange interpolation polynomials in reducing both dissipation and dispersion errors.