• Title/Summary/Keyword: ascomycetes

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Dactylospora glaucomarioides (Ascomycetes, Dactylosporaceae): A Lichenicolous Fungus New to South Korea

  • Joshi, Yogesh;Knudsen, Kerry;Wang, Xin Yu;Hur, Jae-Seoun
    • Mycobiology
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    • v.38 no.4
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    • pp.321-322
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    • 2010
  • The lichenicolous fungi flora of South Korea is poorly known. During recent field trips to various parts of South Korea and after an extensive examination of herbarium lichen specimens, we encountered a lichenicolous fungi growing over a thallus of the lichen Ochrolechia yasudae Vain., characterized by small black apothecia with mostly three-septate brown ascospores. It was identified as Dactylospora glaucomarioides. This is the first report of this lichenicolous fungus from South Korea. A taxonomic description and comments are presented.

Notes on the Korean Ascomycetes (I) (한국산 자낭균류기 (I))

  • DuckHyunCho
    • Korean Journal of Plant Resources
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    • v.9 no.3
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    • pp.291-297
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    • 1996
  • Many Ascomycetes of higher fungi were collected at Mt.Jiri National Park and Mt.Moak Provincial Park from March 1995 to September 1996. They were identified by the keys of Dennis(1981) and Breitenbach and Kranzlin(1984). Of these, Hypoxylon multiforme, H. rubiginosum, H.udum, H.rutilum, Nectria gallignea, N. coccillea, N. pallidula, and N. fumicola were recorded as new to Korea.

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Notes on the Korean Ascomycetes(V) (한국산자낭균류기 (V))

  • 조덕현
    • Korean Journal of Plant Resources
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    • v.10 no.4
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    • pp.369-374
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    • 1997
  • Many higher fungi of ascomycetes were collected at Mt.Jiri National Park, Pyonsan Peninsula National Park, Mt. Pangtae of Kangwon-do, Mt. Moak Provincial Park of Chollabuk-do and Mt. Manduck near Chonju city from June 1995 to October 1996. Identification of the revealed that Calycellina, Diatrype and Diatrypella were new genera to Korea and Scutellinia umbrarum, S. pseudoumbarum, Dasyscyphus virgineus. Calycellina punctiformis, Xylaria oxyacanthae, Diatrype disciformis, Diatrypella quercina and D. verrucaeformis were newly to Korea.

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Shifting reproductive mode of a mycotoxin producing-fungus by manipulation of mating-type genes

  • Lee, Jungkwan;Lee, Teresa;Lee, Yin-Won;Yun, Sung-Hwan;Gillian Turgeon
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 2003.10a
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    • pp.85.1-85
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    • 2003
  • In most ascomycetes, a single mating type locus, MAT, with two alternate forms (MAT1-1 and MAT1-2) called idiomorphs, controls mating ability. In heterothallic ascomycetes these alternate idiomorphs reside in different nuclei. In contrast, most homothallic ascomycetes carry both MAT1-1 and MAT1-2 in a single nucleus, usually closely linked. An example of the latter is Gibberella zeae, a producer of mycotoxins such as trichothecene and zearalenone that threaten human and animal health. We asked if G. zeae could be made strictly heterothallic by manipulation of MAT. Targeted gene replacement was used to differentially delete MAT1-1 or MAT1-2 from a wild type haploid MAT1-1 MAT1-2 strain, resulting in MAT1-1;mat1-2, mat1-1;MAT1-2 strains that were self-sterile, yet able to cross to wild type testers and more importantly, to each other. These results indicated that differential deletion of MAT idiomorphs eliminates selfing ability of G. zeae, but the ability to outcross is retained. To our knowledge, this is the first report of complete conversion of fungal reproductive strategy from homothallic to heterothallic by targeted manipulation of MAT. Practically, this approach opens the door to simple and efficient procedures for obtaining sexual recombinants of G. zeae that will be useful for genetic analyses of mycotoxin production and other traits, such as ability to cause disease.

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Ascophyllum and Its Symbionts. VII. Three-way Interactions Among Ascophyllum nodosum (Phaeophyceae), Mycophycias ascophylli (Ascomycetes) and Vertebrata lanosa (Rhodophyta)

  • Garbary, David J.;Deckert, Ron J.;Hubbard, Charlene B.
    • ALGAE
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    • v.20 no.4
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    • pp.353-361
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    • 2005
  • Ascophyllum nodosum (L.) Le Jolis has a systemic infection with the ascomycete Mycophycias ascophylli (Cotton) Kohlmeyer and Volkmann-Kohlmeyer with which it establishese a mutualistic symbiosis. In addition, A. nodosum is the host for the obligate red algal epiphyte, Vertebrata lanosa (L.) Christensen. Using light and electron microscopy we describe morphological and cytochemical changes occurring as a consequence of rhizoid penetration of V. lanosa into cortical host tissue. Rhizoids induce localized cell necrosis based on physical damage during rhizoid penetration. Host cells adjacent to the rhizoid selectively undergo a hypersensitive reaction in which they become darkly pigmented and become foci for hyphal development. Light and electron microscopy show that M. ascophylli forms dense hyphal aggregations on the surface of the V. lanosa rhizoid and extensive endophytic hyphal growths in the rhizoid wall. This is the first morphological evidence of an interaction between M. ascophylli and V. lanosa. We speculate that M. ascophylli may be interacting with V. lanosa to limit tissue damage to their shared host. In addition, the fungus provides a potential pathway for the transfer of materials (e.g., nutrients and photosynthate) between the two phototrophs.

Formation of Fungal Protoplast by the Reaction of Cell Wall Lytic Enzymes (섬유소 가수분해효소와 Chitinase 처리에 의한 균류 원형질체 생성에 관한 연구)

  • Oh, Sung-Hi;Chang, Myung-Ho
    • Korean Journal of Food Science and Technology
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    • v.13 no.2
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    • pp.101-106
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    • 1981
  • Osmotically sensitive fungal protoplasts were liberated from the mycelium of several kinds of molds by mixed enzyme system of cellulase from Trichoderma viride $TO_4$ and chitinase from the culture filtrate of Streptomyces sp. 115-5. Relatively higher number of protoplast were released from young mycelium of Zygomycetes strains than Ascomycetes strains by using 10 mM phosphate buffer (pH 6.0) and 0.6 M NaCl as osmotic stabilizer. Protoplasts were released through ruptures in the wall, initially at the apices, but later also from old party of the hyphae.

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Germ Tube Formation of Ascospores of Two Terrestrial Higher Ascomycetes, Hypoxylon mammatum and H. truncatum

  • Lee, Yang-Soo;Han, Sang-Sub;Shin, Jong-Ho;Lee, Young-Mi;Song, Bong-Keun
    • Journal of the Korean Wood Science and Technology
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    • v.28 no.4
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    • pp.10-16
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    • 2000
  • Two wood decay ascomycetes fungi identified as Hypoxylon mammatum and H. truncatum were isolated from backyard of Korea Research Institute of Chemical Technology (KRICT) in Korea. Hypoxylon truncatum is newly recorded as a wood degrader in Korea. Unusual germination mechanisms of ascospores in H. mammatum and H. truncatum are described and illustrated. The differences between two species were noticed on the process of germ tube formation. In the process of germ tube formation, the fast movement to pigmented ascospores activated from their perispores was termed as spore eclosion that was only found in H. mammatum. This sophisticated recognition mechanism indicated the existence of specific eclosion and germ tube formation due to the composition of cell wall layers and their preferable host derive, based on examined two species under a genus. The observation on present study postulates different composition of wall layers of ascospore and different nutrient composition for germination.

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Four Endophytic Ascomycetes New to Korea: Cladosporium anthropophilum, C. pseudocladosporioides, Daldinia eschscholtzii, and Nigrospora chinensis

  • Lee, Dong Jae;Lee, Jae Sung;Lee, Hyang Burm;Choi, Young-Joon
    • The Korean Journal of Mycology
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    • v.47 no.3
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    • pp.187-197
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    • 2019
  • Ascomycota is the largest phylum of the Fungi, including approximately 6,600 genera. They are often isolated from soils, indoor air, and freshwater environments, but also from plants as pathogens or endophytes. In this study, four species of Ascomycota (two of Cladosporium and one of each Daldinia and Nigrospora) were collected from the leaves of four woody plants (Camellia japonica, Ginkgo biloba, Quercus sp., Vitis vinifera). Their cultural characteristics were investigated on five different media (PDA, V8A, CMA, MEA, CZA) at 3 days after incubation at $25^{\circ}C$ in darkness. BLASTn search and phylogenetic analysis were performed using the internal transcribed spacer (ITS) rDNA sequences, in addition to tef1 gene sequences for Cladosporium species. Based on the cultural, morphological, and phylogenetic data, the isolates were identified as Cladosporium anthropophilum, Cladosporium pseudocladosporioides, Daldinia eschscholtzii, and Nigrospora chinensis. Previously, some members of Cladosporium and Nigrospora have been recorded as endophytes inhabiting the leaves and stems of various plants, whereas Daldinia eschscholtzii is a wood-inhabiting endophyte or wood-decaying fungus. To our knowledge, this is the first report of these four ascomycetes in Korea.