• Title/Summary/Keyword: artificial source

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Selection of Superior Strains from Collected Ear Mushrooms for Artificial Cultivation and Their Optimal Condition of Mycelial Growth (목이류 인공재배를 위한 우량균주 선발 및 균사생장 최적 조건 구명)

  • Kim, Jeong-Han;Lee, Yun-Hae;Jang, Myoung-Jun;Won, Seon-Yi;Ju, Young-Cheoul
    • The Korean Journal of Mycology
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    • v.42 no.1
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    • pp.57-63
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    • 2014
  • This study was carried out to obtain morphological and physiological characteristics of ear mushrooms for an artificial cultivation. Eighteen strains were cultivated with bag culture and classified into mainly five groups such as brown, black, white, purple and others group. The highest yield was shown in 43007 strain as 98.3 g/bag and 43009, 43016, 43025 and 44035 strains were more than 60 g/bag. Among collected strains, 43007, 43009 and 43035 were selected in this study as superior strains. Three selected strains were investigated for optimal mycelial growth conditions. MCM and GPYM media were selected for the favorable culture medium. The carbon sources of 43007, 43009 and 43035 on mycelial growth were maltose, fructose and glucose, respectively and peptone was selected as a nitrogen source. Highest mycelial growth was observed when the C/N ratio was 10 for 43007 and 20 for 43009 and 43035.

Studies on the Artificial Cultivation of Morchella esculenta in Ascomycetes (자낭균 곰보(Morchella esculenta)버섯의 인공재배에 관한 연구)

  • Kim, Han-Kyoung;Lee, Kang-Hyo;Cheong, Jong-Chun;Jhune, Chang-Sung;Seok, Sun-Ja;Jang, Kab-Yeul
    • Journal of Mushroom
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    • v.7 no.1
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    • pp.9-21
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    • 2009
  • This study was executed in an attempt to investigate a artificial requisites of fruitbody occurrence. Environmental requirements on habitat for fruitbody occurrence of collected cultures resulted in leading to $13-16^{\circ}C$ and 75% relative humidity, and requiring silt loam of soil texture which had more nutritional substances than a dry field. Optimal temperature was $25^{\circ}C$, medium PDB, and pH 5.0 in cultural conditions. Mannose required of 5% in ASI 59002, 59003, 59004, but 3% in ASI 59001 was selected as optimum carbon source. The substrates stimulating sclerotium formation were cotton waste, or cotton waste + oak sawdust (mixture ratio of 8:2), which had 20% additive of wheat barn respectively. Sclerotium was formed well in the substrate adjusted chemical properties by applying 2% of calcium sulfate. Sclerotium formation was the most effective in the treatment of peat moss + oak sawdust (mixture ratio of 5:5) + 30% of wheat barn.

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Biological Treatment of Wastewater Containing Chlorinated Phenols by a Mixed Culture (복합미생물제재를 이용한 염소화 페놀계 폐수의 생물학적 처리)

  • 오희목;이완석;정상욱;박찬선;윤병대;김장억
    • Microbiology and Biotechnology Letters
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    • v.29 no.2
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    • pp.115-121
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    • 2001
  • Biological Treatment of Wastewater Containing Chlorinated Phenols by a Mixed Culture. Lee, Wan-Seok1, Sang-Wook Jung, Chan-Sun Park, Byung-Dae Yoon, Jang-Eok Kim\ and Hee-Mock Oh*. Environmental Bioresources Laboratory, Korea Research Institute of Biosicence and Biotechnology, Taejon, Korea, 1 Department of Agricultural Chemistry, Kyungpool< National University, Taegu, Korea - The biodegradation of chlorinated phenols in an artificial wastewater was investigated using a mixed culture. The mixed culture was composed of 8 microorganisms isolated from the soil contaminated with various chlorinated phenols. Pseudomonas sp. BM as a main constituent of a mixed culture was Gram-negative, catalase- and oxidase-positive, and rod-shaped, and did not grow at 41°C. It degraded 99% of initial 500 mg!1 of pentachlorophenol (PCP) in the minimal salts medium as a sole source of carbon and energy within 3 days. The degradation efficiency of Pseu.domon.as sp. BM was not affected by the other organic carbon and nitrogen compounds. Pseudomonas sp. BM was able to grow in a broad range of pH 5 - 8, and degrade 2,000 mg/1 PCP. In the experiment with an artificial wastewater containing chlorinated phenols, the degradation efficiency of the mixed culture was the range of 73% (2,4-dichlorophenol) -96% (2-chlorophenol) during an incubation of 7 days. In a continuous culture experiment, the degradation efficiency of mixed culture plus activated sludge was about 2 times higher than that of the control containing only activated sludge. These results indicate that it is possible to apply the mixed culture to other wastewaters containing chlorinated phenols. Key words: Biodegradation, chlorinated phenols, pentachlorophenol, Pseudomonas sp. BM

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Experimental study on Light and Gas Pollution Resistance of Commercial Natural Pigments for Dancheong - Focucing on Korea, Japan and China Products - (시판 단청용 천연안료의 내광성·내공해성 실험 연구 -한국, 일본, 중국 생산 안료를 중심으로-)

  • Lee, Kyeong Min;Kim, Soon Kwan;Bae, Su Bin;Kim, Mi Jeong
    • Journal of Conservation Science
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    • v.31 no.4
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    • pp.443-455
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    • 2015
  • To verifying the stability of Natural pigments for Dancheong which is available on the market was carried out as light resistance and gas corrosion test. In this particular case, we will confirm the characteristics of the only pigments except of Dancheong technique and influence of substance. Medium using for coloring are animal glue and synthetic resin that widely used for Dancheong in currently. Selected to artificial light source is a xenon arc lamp, contaminated gas is nitrogen dioxide gas in experiment. Degree of degradation of pigment were compared by measuring the degree of color change. The result of light resistance test, mineral pigments were good; color is green and blue type, soil pigments also good; color is yellow ocher, white clay, red clay etc. Gamboge, unghwang, cinnabar, red lead were confirmed not good. The result of gas corrosion test, most of the natural pigments were good condition except the red lead. Generally, animal glue samples had better than a synthetic resin samples.

Prediction of multipurpose dam inflow using deep learning (딥러닝을 활용한 다목적댐 유입량 예측)

  • Mok, Ji-Yoon;Choi, Ji-Hyeok;Moon, Young-Il
    • Journal of Korea Water Resources Association
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    • v.53 no.2
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    • pp.97-105
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    • 2020
  • Recently, Artificial Neural Network receives attention as a data prediction method. Among these, a Long Shot-term Memory (LSTM) model specialized for time-series data prediction was utilized as a prediction method of hydrological time series data. In this study, the LSTM model was constructed utilizing deep running open source library TensorFlow which provided by Google, to predict inflows of multipurpose dams. We predicted the inflow of the Yongdam Multipurpose Dam which is located in the upper stream of the Geumgang. The hourly flow data of Yongdam Dam from 2006 to 2018 provided by WAMIS was used as the analysis data. Predictive analysis was performed under various of variable condition in order to compare and analyze the prediction accuracy according to four learning parameters of the LSTM model. Root mean square error (RMSE), Mean absolute error (MAE) and Volume error (VE) were calculated and evaluated its accuracy through comparing the predicted and observed inflows. We found that all the models had lower accuracy at high inflow rate and hourly precipitation data (2006~2018) of Yongdam Dam utilized as additional input variables to solve this problem. When the data of rainfall and inflow were utilized together, it was found that the accuracy of the prediction for the high flow rate is improved.

Comparison of mycorrhizal fungi associated with Pinus species in cultural characteristics and artificial mycorrhizal synthesis on Pinus thunbergii seedlings (소나무류 균근균의 배양적 특성비교 및 인공접종에 의한 해송묘목에의 균근협성)

  • Lee, Jong Kyu;Lee, Hoon Yong;Lee, Sang Yong
    • Journal of Forest and Environmental Science
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    • v.15 no.1
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    • pp.77-88
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    • 1999
  • This experiment was carried out to compare the cultural characteristics of mycorrizal fungi associated with Pinus species, and to form mycorrhizal association with Pinus thunbergii by artificial inoculation of these fungi. Mycorrhizal fungi tested showed great variations in cultural characteristics. Most fungal isolates was best grown on MP medium, except PDA for Lepista sp.(Ln73/92). Hagem for Rhizopogon rubescens(FRI91017), and FDA for Paxillus sp.(Pa60/92). Optimum temperature for these fungi was $25^{\circ}C$, except $30^{\circ}C$ for Pisolithus tinctorius(FRI91004 and Pt1). The range of pH conditions favorable for these fungal isolates were also variable from weak acidic(pH5) to weak alkalic(pH8). Utilization of the carbon sources for these mycorrhizal fungi was different. Fructose, glucose, and maltose were all utilized well, while xylose was not utilized generally. Mycelial growth on the media supplemented with potassium nitrate was better than those on other media with urea, asparagine, or peptone as a nitrogen source, and the poor growth was observed on the media with urea. Pisolithus tinctorius(Pt1) among 7 mycorrhizal fungi artificially inoculated for the mycorrhizal synthesis on pinus thunbergii seedlings in the test tube containing a mixture of peat moss-vermiculite(2:1, v/v) formed mycorrhizae successfully after 3 months. P. tinctorius formed branched and unbranched roots covered with thick fungal mantle and radiating extemal hyphae. Mycorrhizal root cross-sectioned by hand, stained, and observed by Nomarski interference microscope showed typical characteristics of ectomycorrhizae: fungal mantle on epidermal cells and thick Hartig net hyphae around cortex cells.

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Predicting the Potential Distribution of Korean Pine (Pinus koraiensis) Using an Ensemble of Climate Scenarios (앙상블 기후 시나리오 자료를 활용한 우리나라 잣나무림 분포 적지 전망)

  • Kim, Jaeuk;Jung, Huicheul;Jeon, Seong Woo;Lee, Dong-Kun
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.18 no.2
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    • pp.79-88
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    • 2015
  • Preparations need to be made for Korean pine(Pinus koraiensis) in anticipation of climate change because Korean pine is an endemic species of South Korea and the source of timber and pine nut. Therefore, climate change adaptation policy has been established to conduct an impact assessment on the distribution of Korean pine. Our objective was to predict the distribution of Korean pine while taking into account uncertainty and afforestation conditions. We used the 5th forest types map, a forest site map and BIOCLIM variables. The climate scenarios are RCP 4.5 and RCP 8.5 for uncertainty and the climate models are 5 regional climate models (HadGEM3RA, RegCM4, SNURCM, GRIMs, WRF). The base period for this study is 1971 to 2000. The target periods are the mid-21st century (2021-2050) and the end of the 21st century (2071-2100). This study used the MaxEnt model, and 50% of the presences were randomly set as training data. The remaining 50% were used as test data, and 10 cross-validated replicates were run. The selected variables were the annual mean temperature (Bio1), the precipitation of the wettest month (Bio13) and the precipitation of the driest month (Bio14). The test data's ROC curve of Korean pine was 0.689. The distribution of Korean pine in the mid-21st century decreased from 11.9% to 37.8% on RCP 4.5 and RCP 8.5. The area of Korean pine at an artificial plantation occupied from 32.1% to 45.4% on both RCPs. The areas at the end of the 21st century declined by 53.9% on RCP 4.5 and by 86.0% on RCP 8.5. The area of Korean pine at an artificial plantation occupied 23.8% on RCP 4.5 and 7.2% on RCP 8.5. Private forests showed more of a decrease than national forests for all subsequent periods. Our results may contribute to the establishment of climate change adaptation policies for considering various adaptation options.

Development of Descending Thoracic Aortomyoplasty for Cardiac Bioassist (심장보조를 위한 흉부대동맥 근성형술 개발(예비 동물실험))

  • 오중환;박승일;김은기;김영호;류기홍;이상헌;원주호;서재정
    • Journal of Chest Surgery
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    • v.33 no.6
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    • pp.469-475
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    • 2000
  • Background: Thoracic aortomyoplasty is one of the surgical treatment for heart failure and has advantages over artificial heart or intraaortic balloon pumps. It uses autogenous skeletal muscles and solves problems such as energy source. However its use in clinical settings has been limited. This preliminary study was designed to develop surgical technique and to determine the effect of acute descending thoracic aortomyoplsty. Material and Method: Thirteen adult Mongrel dogs were used. The left latissimus dorsi muscle was wrapped around the descending aorta under general anesthesis. Swan-Ganz and microtipped Millar catheter were used for the hemodynamics and endocaridial viability ratio. Data were collected with myostimulator on and off in normal hearts and the ischemic hearts. Result: In normal hearts, the mean aortic diastolic pressure increased from 72$\pm$15mmHg at baseline to 78$\pm$13mmHg with stimulator on. Coronary perfusion pressure increased from 61$\pm$11mmHg to 65$\pm$9mmHg. Diastolic time increased from 0.288$\pm$0.003 msec to 0.290$\pm$0.003msec. Systolic time decreased from 0.164$\pm$0.002msec to 0.160$\pm$0.002 msec. Endocardial viability ratio increased from 1.21$\pm$0.22 to 1.40$\pm$0.18. In ischemic hearts, mean aortic diastolic pressure incrased from 56$\pm$21mmHg at baseline to 61$\pm$15mmHg with stimulator on. Coronary perfusion pressure increased from 48$\pm$17mmHg to 52$\pm$15mmHg. Diastolic time increased from 0.290$\pm$0.003 msec to 0.313$\pm$0.004msec. Systolic time decreased from 0.180$\pm$0.002 msec to 0.177$\pm$0.003 msec. Endovascular viability ratio increased from 0.9$\pm$0.31 to 1.1$\pm$0.31. The limited number of cases ruled out the statistic significance. Conclusion: Descending thoracic aortomyoplasty is a simple operation designed to use patient's own skeletal muscles. It trends to increase diastolic augmentation and coronary perfusion pressure. Modification of surgical technique and stimulator protocol would maximize the effect to assist the heart.

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Artificial Intelligence to forecast new nurse turnover rates in hospital (인공지능을 이용한 신규간호사 이직률 예측)

  • Choi, Ju-Hee;Park, Hye-Kyung;Park, Ji-Eun;Lee, Chang-Min;Choi, Byung-Gwan
    • Journal of the Korea Convergence Society
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    • v.9 no.9
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    • pp.431-440
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    • 2018
  • In this study, authors predicted probability of resignation of newly employed nurses using TensorFlow, an open source software library for numerical computation and machine learning developed by Google, and suggested strategic human resources management plan. Data of 1,018 nurses who resigned between 2010 and 2017 in single university hospital were collected. After the order of data were randomly shuffled, 80% of total data were used for machine leaning and the remaining data were used for testing purpose. We utilized multiple neural network with one input layer, one output layer and 3 hidden layers. The machine-learning algorithm correctly predicted for 88.7% of resignation of nursing staff with in one year of employment and 79.8% of that within 3 years of employment. Most of resigned nurses were in their late 20s and 30s. Leading causes of resignation were marriage, childbirth, childcare and personal affairs. However, the most common cause of resignation of nursing staff with in one year of employment were maladaptation to the work and problems in interpersonal relationship.

Study on Sensitivity of Variables of the Experiment to Evaluate Discomfort Glare of Interior Artificial Illumination (건축실내 인공조명의 불쾌글레어 평가실험에 적용된 실험변수의 민감도 분석에 관한 연구)

  • Lee, Jin-Sook;Kim, Won-Do;Kim, Chang-Soon
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.22 no.1
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    • pp.24-32
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    • 2008
  • The ultimate purpose of the study is to develop a discomfort glare forecasting formula that can be practically used in Korea in order to effectively forecast discomfort glare considering the optical characteristics of the Koreans. The study was to examine the relations between discomfort glare and the variables such as luminance, background luminance, solid angle, luminous area and louver. To this end, experiments were conducted in a mock-up office that emulates the lighting environment of an ordinary office. The study was conducted by four steps as follow. First, previous studies on discomfort glare rating to define and rate discomfort glare were analyzed and modified to be applied to the experiments of this study. Second, experiment variables, variable scope, evaluation objects and evaluation points were determined after review on existing discomfort glare evaluation experimental formulas. Third, experiments were conducted in a mock-up office to be able to control variables. Finally, sensitivity of experiment variables were analyzed through examination of the relation between discomfort glare and the variables such as luminance, solid angle, louver, luminous area and subject's position. The result showed that the most influential variables on discomfort glare of an artificial light source is luminance and louver, luminous area and subject's position(solid angle) followed.