• Title/Summary/Keyword: artificial crosses

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Parental Selection Strategies in Plant Breeding Programs

  • Bertan, Ivandro;Carvalho, Fernando I. F. de;Oliveira, Antonio Costa de
    • Journal of Crop Science and Biotechnology
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    • v.10 no.4
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    • pp.211-222
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    • 2007
  • Selection of the appropriate parents to be used in artificial crosses is one of the main decisions faced by plant breeders that will facilitate the exploitation of maximum genetic variability and production of superior recombinant genotypes. Several techniques have been used in aiding the identification of genotypes with promising and desirable agronomical traits for hybridization. In this way, the objective of the present review is to gather available information for the selection of parents based on different breeding designs and analytical tools showing their similarities and highlighting the main advantages and disadvantages of their use.

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Artificial and Natural Selection for Phototactic Behavior in Drosophila melanogaster (초파리의 走光性行動에 대한 人爲淘汰와 自然淘汰)

  • Choo, Jong-Kil
    • The Korean Journal of Zoology
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    • v.20 no.1
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    • pp.1-8
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    • 1977
  • Several hundred flies of Drosophila melanogaster collected in Anyang City were selected for positive, negative and neutral directions during 15 generations. The population responded effectively to the artificial selection. The realized heritability estimated for the first 10 generations was $2 \\sim 4%$ per generation in the positive and negative phototaxis. The results of diallel crosses among selected populations indicated that some polygenes showing a negative phototaxis were partially dominant over polygenes controlled the positive phototaxis, and the dominant effect became greater in later generations. The populations selected for positive and negative phototaxis were relaxed after 15 generations of selection, and their phototactic responses were completely returned to their original states. Such phenomena would be explained by the genetic homeostasis resulted from an action of natural selection. It seems reasonable to assume that the phototactic neutrality of a natural population was maintained as an adaptive trait under natural environment.

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Hybridization and Use Of Grapes as an Oviposition Substrate Improves the Adaptation of Olive Fly Bactrocera oleae (Rossi) (Diptera: Tephritidae) to Artificial Rearing Conditions

  • Sohel, Ahmad;Viwat, Wornoayporn;Polychronis, Rempoulakis;Emily A., Fontenot;Ul Haq, Ihsan;Carlos, Caceres;Hannes F., Paulus;Marc J.B., Vreysen
    • International Journal of Industrial Entomology and Biomaterials
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    • v.29 no.2
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    • pp.198-206
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    • 2014
  • The olive fly Bactrocera oleae (Rossi) is the key pest for olive cultivation worldwide. Substantial effort has been invested in the development of the sterile insect technique (SIT) to control this pest. One of the limitations to develop SIT technology for olive fruit fly is the low ability of wild females to lay eggs in other medium than olive fruits, and their slow adaptation to oviposition in artificial substrates. In the present study, fruit grapes were used as an alternative egg collection medium to harvest eggs and young larvae from freshly colonized wild strains originating from France, Italy, Spain and Croatia. The larvae were allowed to develop into the fruits until the second instar, before they were extracted out and further reared on a standard artificial diet. Furthermore, F1 to F4 female flies were alternatively offered wax bottles to oviposit. Finally, the performance of hybrid strains created from crosses between wild and long colonised flies was assessed. The results showed that females of all 4 wild strains readily oviposited eggs in grapes and from the F2 generation onward, females from all strains were adapted to laying eggs in wax bottles. No difference was observed in eggs and pupae production among all strains tested. The findings are discussed for their implications on SIT application against olive fruit fly.

Identification of Heterosis QTLs for Yield and Yield-Related Traits in Indica-Japonica Recombinant Inbred Lines of Rice (Oryza sativa L.)

  • Kim, Chang-Kug;Chu, Sang-Ho;Park, Han Yong;Seo, Jeonghwan;Kim, Backki;Lee, Gileung;Koh, Hee-Jong;Chin, Joong Hyoun
    • Plant Breeding and Biotechnology
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    • v.5 no.4
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    • pp.371-389
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    • 2017
  • Supplying sufficient rice to growing populations is a global challenge. Hybrid indica rice varieties exploiting heterosis have increased yields, but inter-subspecific crosses between indica and japonica varieties are hampered by sterility. Examination and genetic understanding of yield heterosis in indica/japonica crosses addressing yield barriers are basic requirements. In this study, QTLs for heterosis of yield traits were identified in indica-japonica recombinant inbred lines (RILs) using a total of 178 RILs originating from Dasanbyeo (indica) ${\times}$ TR22183 (japonica) (DT-RILs) and their backcrossed populations. Nine of sixty-six major quantitative trait loci (QTLs) identified in DT-RILs exhibited heterosis. Heterosis QTLs clustered with other traits on chromosomes 1, 4, and 8, and clusters were conserved between different RILs. The clusters contained several known yield enhancement genes/QTLs. Specific heterotic allele combinations contributed to four major heterosis QTLs, particularly for panicle and spikelet number traits. Heterosis for yield and yield-related traits was explained by the harmonized effects of overdominance, dominance, and epistatic interactions in inter-subspecific breeding populations.

A comparative study on the accuracies of resin denture bases and metal denture bases

  • Park Hwee-Woong;Kim Chang-Whe;Kim Yung-Soo
    • The Journal of Korean Academy of Prosthodontics
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    • v.39 no.3
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    • pp.250-259
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    • 2001
  • Since the late 1930s, acrylic resins have been the materials of choice for the fabrication of complete denture bases. It has excellent esthetic properties, adequate strength, low water sorption, and low solubility. But acrylic resin has disadvantage of processing shrinkage that reduces denture retention and accuracy of denture occlusion. Metals also have been used in denture base material. Metals used in denture bases display excellent strength and dimensional stability. The major disadvantages associated with metal denture bases include increased cost, difficulty in fabrication, compromised esthetic qualities, and inability to re-base. The purpose of this study is to compare the artificial tooth movements of complete dentures with resin bases and metal bases after curing, deflasking, polishing immersion in water for 1 week and 4 weeks. Twenty-four maxillary complete resin denture bases with artificial teeth were fabricated. Twelve of them were resin based and other twelve of them were metal based. Fine crosses were marked on the incisal edges of right central incisors and distobuccal cusps of be second molars. Measurements were done for the changes of distances of reference points at the time of wax denture, after deflasking after decasting after polishing after immersion in water for 1 week and 4 weeks Meaurements were done to the accuracy of 0.001mm with a measuring microscope. The results were as follows : 1. Metal base showed significantly less tooth movement than resin base after curing and decasting (p<0.01). 2. Metal base showed significantly less tooth movement than resin base after polishing (p<0.01). 3. After immersion in water for 1 week and 4 weeks, metal base showed less movement than resin base. Difference was significant for anterior-posterior distances (p<0.01), but not significant for molar-to-molar distance (p>0.01). 4. 1 week and 4 weeks of immersion failed to compensate the initial processing shrinkage of metal and resin bases (p>0.01).

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Overcoming F1 Sterility of Intersectional OA Lily Hybrids by Somatic Chromosome Doubling and Production of Subsequent Progeny (체세포 염색체 배가법을 이용한 백합 OA 종간 잡종의 불임극복과 후대획득)

  • Chung, Mi-Young;Chung, Jae-Dong;Jee, Sun-Ok;Tuyl J. M. Van;Lim, Ki-Byung
    • Journal of Plant Biotechnology
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    • v.31 no.2
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    • pp.103-108
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    • 2004
  • This study was carried out to develop the efficient methods for the subsequent progeny of intersectional hybrid between Oriental and Asiatic lily hybrids. The pollen fertility and germination ability of 3 different allotetraploids (OAOA) after somatic chromosome doubling was ranged from 0 to 80 percent on artificial pollen germination medium. The number of BC$_1$ progeny using allotetraploid of F$_1$ OA-hybrid as male and female parent was different. The efficiency of BC$_1$ progeny production was increased when F$_1$OA-hybrids was used as male rather than as female parent. And in back crosses of F$_1$ OA-hybrids with the Asiatic and Oriental hybrids, Asiatic hybrids showed higher efficiency on BC$_1$ progeny production. The ploidy level between 2x or 4x Asiatic hybrid and allotetraploid F$_1$ OA-hybrid was determined and showed higher progeny production in 2x-4x crosses rather than 4x-4x.

Studies on the Effect of Temperature During the Reduction Division and the Grain Filling Stage in Rice Plants II. Effect of Air Temperature at the Grain Filling Stage in Indica-Japonica Crosses (수도의 감수분열기 및 등숙기에 있어서 온도반응에 관한 연구 제2보 수도 Indica$\times$Japonica 품종의 등숙기에 있어서의 온도반응)

  • kyu-Chin Kim
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.28 no.1
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    • pp.58-75
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    • 1983
  • The effects of air temperature on the grain filling stage of indica-japonica crosses were studied in artificial temperature-controlled cabinets, using Indica-Japonica crosses (Suweon 264, Suweon 258 and Milyang 29), Indica IR 36 and Lengkwang) and a Japonica rice (Jinheung). The optimum temperature to achieve maximum grain weight during the grain-filling stage was $26/18^{\circ}C$ for all varieties. Within the temperature range of 13 to $28^{\circ}C$, the grain filling period was shorter as the temperature was increased. At the highest temperature $(32/24^{\circ}C)$ the upper-position grains ("special spikelets") of IR 36 required 13days after flowering to reach the maximum weight, in Jinheung 23 days, in indica-japonica varieties (Suweon 264, Suweon 258, and MiIyang 29) 18 days, and in Lengkwang, 23 days. In the case of Lengkwang, at $32/24, \;29/21^{\circ}C$ and $26/18^{\circ}C$temperature range the upper-position grain also required same 23 days after flowering to reach the maximum weight. At the lowest temperature range of $17/9^{\circ}C$, Jinheung required 68 days and Lengkwang 53 days after flowering to reach maximum grain weight. The whole panicle took 10 to 15 days later than the special spikelets to reach the maximum weight. At lower-than-optimum temperature range $(l7/9^{\circ}C\;and\;20/12^{\circ}C)$, all IR 36 plant died within 15 days after flowering. In the case of indica-japonica varieties all plants died within 15 days only at $17/9^{\circ}C$. There were more chalky grains when the temperature was higher and lower than optimum. The highest and lowest temperature range produced dead rice (black and dark red rice). Lower than optimum temperature ranges $(17/9^{\circ}C\;and\;20/12^{\circ}C)$ affected grain quality (length, width and depth) at these temperature ranges, grains were shorter, narrower, and thinner than grains at the optimum temperature of $26/18^{\circ}C$. With regared and the effects of temperature on frequency of grain weight at grain filling stage of indica variety IR 36, the highest $(32/24^{\circ}C)$ and lowest $(23/15^{\circ}C)$ temperature ranges matched the frequency of grain weight curves. In Japonica variety Jinheung, at the three temperature ranges, that is, lowest $(17/9^{\circ}C)$ highest $(32/24^{\circ}C)$ and optimum $(26/18^{\circ}C)$, the frequency of grain weight curve showed very different patterns, In the case of indica-japonica variety Suweon 258, the frequency of grain weight curve was midway between that of IR 36 and Jinheung. Jinheung.

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Studies on the Effects of Temperature During the Reduction Division and the Grain Filling Stage in Rice Plants I. Effect of Temperature at the Reduction Division Stage in Indica-Japonica Crosses (수도의 감수분열기 및 등숙기에 있어서 온도반응에 관한 연구 제1보 수도 indica $\times$ Japonica품종의 감수분열기에 있어서의 저온의 영향)

  • Kim, Kyu-Chin
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.28 no.1
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    • pp.41-57
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    • 1983
  • The effect of temperature 'during the reduction division stage on the Indica-Japonica rice varieties were studied in artificial temperature-controlled cabinets (Bioclimatic Laboratory). Varieties used were indica-japonica crosses (Suweon 264, Suweon 258, Milyang 29), indica (IR36, Lengkwang) and japonica rice (Jin-heung). The results obtained from this study are summarized as follows. The most sensitive stage to low temperature, the tetrad stage, was observed in each variety with the following auricle distance: Jinheung, -12cm; IR36, -3cm; Suweon264, -9cm; Suweon258, -3cm; Milyang29, -6cm and Lengkwang, +1cm. The tetrad stage occured when the palea elongation was about 50 to 60% of the final palea length at flowering stage in all varieties. The percentage of degenerated spikelets at tetrad as influenced by low-temperature treatment was very low in Jinheung and Lengkwang, but very high in IR36. In indica-japonica crosses only the 7-days treatments had a very high percentage. Between the 4-day and 7-day treatments as well as among all varieties, clear differences were observed in the low temperature induced sterility of 'special spikelets' (upper position grains at tetrad). In the 7-day treatments except in Lengkwang, all varieties showed very high sterility. It appears that the treatments is over the critical limit for treatment. However in the 4-day treatments, the intervarietal differences in sterility were very clear. In Jinheung, sterility was 46.8%; IR36, 67.6%; Suweon264, 60.9%, Milyang29, 62.2%; Lengkwang, 27.8%. A close relationship was observed between fertility and auricle distance. The lowest fertility of special spikelets was at the tetrad stage and the first contraction phase. Before and after the stage there was an increase in fertility. The palea length elongated nearly in a straight line from the spikelet formation stage to flowering. And there was a high correlation between palea length and auricle distance. distance.

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Expanded Role of Empathy in Metaverse Environment Where AI is Applied (AI가 적용될 메타버스 시대를 위한 확장된 공감의 역할)

  • Rhee, Hyunjung
    • The Journal of the Korea Contents Association
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    • v.21 no.11
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    • pp.87-99
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    • 2021
  • As face-to-face social exchanges are limited by the pandemic, the influence of the metaverse, a virtual space crosses virtual and reality, is gradually expanding. Various metaverse platforms are currently taking advanced AI technology to provide users with a more diverse experience. However, as a result, if AI-human communion in the metaverse space is as active as the relationship between humans, this may lead to side effects that threaten humanity. This study emphasized the value of empathy as a way to minimize the side effects, and suggested that the concept and role of empathy need to be expanded. Accordingly, we conducted an empirical study to examine the difference between social relationship in the metaverse and in real life at the current level, and based on the results, we predicted the future social relationship in the environment where AI was applied to the metaverse. Combining the study, we proposed "Digital Human Empathy" for human dignity in the digital age.

Breeding of New Varieties by Ovule Culture of Intergeneric Hybrid in the Aurantioideae (속간교잡 후 배주배양에 의한 감귤류 신품종 육성)

  • 이만상
    • Korean Journal of Plant Tissue Culture
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    • v.22 no.5
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    • pp.261-266
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    • 1995
  • This study was carried out to develop new varieties which are dwarf and tolerant to winter cold in the Aurantioideae by intergeneric crossing. to do that, the reciprocal crosses of Hwanggeumyooza and trifoliate orange, yooza and trifoliate orange were done and in vitro immature ovule culture of their hybrid was carried out .The callus formation from immature ovule was good in order of Hwanggeumyooza, Hwanggeumyooza $\times$ tifoliate orange, yooza, and trifoliate orange and best at 1 to 3 mg/L NAA+0.5mg/L zeatin on MT medium. In vitro germination percentage of 20week old hybrid of Hwanggeumyooza $\times$ tifoliate orange and trifoliate orange $\times$ Hwanggeumyooza were 41.3% and 37.7, respectively. The phenotype of hybrid (95%) of Hwanggeumyooza $\times$ trifoliate orange and that (100%) of trifoliate orange $\times$ Hwanggeumyooza were similar to that of trifoliate orange. After Hwanggeumyooza was pollinated by pollens of trifoliate orange, the pollen tubes grew on stigma after 3h of pollination and entered into micropyle after about 24~28 h. One gamete in pollen was fused with polar nuclei after 2 days and other one fused with egg nucleus at 3days after pollination. The fruit set percentage by intergeneric crossing was 14.0% in Hwanggeumyooza $\times$ trtfoliate orange and 17.5% in trifoliate orange $\times$ Hwanggeumyooza. The fruit set percentages of Hwanggeumyooza. and trifoliate orange were 34.2% and 39.5% by artificial self-fertilization, 34.2% and 39.5% by artificial cross fertilization, 3.1% and 1.4% by parthenocarpy and 13.0% and 3.0% by natural fertilization, respectively. The somatic and gametic chromosome numbers of Hwanggeumyooza, yooza, and trifoliate orange were 2n=18 and n=9.

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