This study describes plant regeneration from leaf explant of Bupleurum falcatum through somatic embryogenesis, and the effect of 2,4-dichlorophenoxyacetic acid on somatic embryo abnormalities. The relationship between the cotyledon number of somatic embryo and its germinability is also described. Embryogenic calli were selected from calli formed on explants cultured on MS solid basal medium supplemented with 1 mg/L 2,4-D. Cotyledonary abnormalities were observed in somatic embryos which were developed from calli cultured on MS medium with 1 mg/L 2,4-D for 6-week and then subcultured on 2,4-D free MS medium for 3 weeks. The frequency of abnormalities was as follows: 7% of somatic embryos had one cotyledon, 65% of them had two cotyledons, 25% three cotyledons, 5% four cotyledons, 2% five cotyledons, and 3% trumpet-like cotyledons. The two cotyledon somatic embryos were germinated at a frequency of 80%. However the germination frequency of one cotyledon embryo and multicotyledonary embryo was lower than that of the two cotyledon somatic embryo. All of trumper-like somatic embryos did not germinate. Histological observations of multicotyledon embryo showed circular procambium in the root but pocambial strands in the cotyledonary node or upper hypocotyl. The number of the strands was equal to the cotyledon number.
This paper reviews battering, sexual violence and divorce experienced among women. There three problems have negative influence on health status in women and are further developed to social problems such as family dissolution. The victims of the problems may manifest physical injury, emotional difficulties and social withdrawl, while their children may show problems caused by lack of parental caring and by resembling abusive behaviors of their parents. Hence, nurses need to pay attention to batting, sexual violence, and divorce and to develop relevant nursing interventions for them. Some strategies of dealing with those problems are presented in the following. First, we have to eliminate sexism prevailing in out society. Our society is assigning inequal and asymmetrical gender role. Mass media should inspire equality between genders and show a healthy model of family and community. Second, social system and laws should be changed through collective efforts. Those living conditions of women cannot be changed by the effort of women themselves only. We all need to work for establishing and changing the law, so that those women in suffering can obtain immediate and adequate protection. Third, social support system of consulting and referring women's problems should be established. Such support system as hot line, shelter and counseling clinics would help women in crisis. Fourth, job training and arrangement should be available to women who are divorced. Fifth, there should be self-help group for those women in suffering. Self-help group would help those women in sharing their problems and feelings and in establishing coping strategies. Nurses, as the largest group among health professionals, are sensitive and respond to health needs of clients and have an effect on managing women's health. However, we nurses have not been ready for dealing with problems of women, although most of us are women. we not need to change our perspective of women's health problems from a traditional medical perspective to feministic one. Accordingly, nurses need to develop realistic way of caring those women in suffering and to assist them in making decisions for their lives by themselves.
The thermotropic liquid crystalline behavior of the homologous series of combined-type liquid crystalline polymers, tri-O-{4-(4'-cyanophenylazo)phenoxy}alkyl celluloses (CACETn, where n, the number of methylene units in the spacer, is 2~10) have been investigated. The CACETn with n of 5 and 7 exhibited enantiotropic nematic phases, while other polymers showed monotropic nematic phases. The isotropic-nematic transition temperature($T_{iN}$) increased when n is increased up to 4, but it decreased with increasing n more than 5. The entropy change at $T_{iN}$ also reaches a minimum at n=5, before it increases again for n=6. The sharp change at n=5 may be attributed to the difference in arrangement in the side groups. The nematic-crystalline transition temperatures, in contrast with $T_{iNS}$, exhibited a distinct odd-even effect, suggesting that the average shape of the side chains in the crystalline phase is different from that in the nematic phase. The mesophase properties of CACETn were significantly different from those reported for tri-O-alkyl celluloses and poly[1-{4-(4'-cyanophenylazo)phenoxyalkyloxy}ethylene]s. The results were discussed in terms of the difference in the chemical structures of the main and side chains and the number of the mesogenic units per repeating unit.
Many of penthouse apartments began to be built and distributed in Korea from the 2000s. However, we have a transformed definition from the international concept of penthouse. It seems that the penthouse apartments in Korea have a confined definition as a luxury and privileged house-we often find it out from advertisements of newly built apartments. In this thesis, therefore, it needs to make a clear and substantial definition of penthouse. And on the base of this definition, this study is intended to analyze planes of penthouse apartments which are distributed recently. It is in order to find out positive aspects of penthouse apartments and to make practical application of this analysis. The process of this study for plane-planning is as follows. Firstly, it is investigated and analyzed that external factors that have an effect on plane-planning such as locations and types of penthouse apartments. Secondly, an interior of penthouse apartments are divided into a private, public and outward area. And properties of each area through this research show the way of division and arrangement of plane. Thirdly, methods of connection between spaces is analyzed on the base of results of research for the way of partition. Finally, specific characters of penthouse apartments planning is derived from synthesis of research. In conclusion, analysis of plane-planning of penthouse apartments is summarized as follows. The majority of penthouse apartments is arranged to small portions of households and on higher stories in apartment which is situated around Han River or public parks. These external factors influence the plane-planning in both the interior and exterior way. For the exterior planning in space, it becomes important to design bays as many as possible in contact with outside in order to satisfy demands for fine prospects. It is also important to plan extra places naturally such as a terrace in order to provide virtual grounding. In the interior planning, a large size of interior induces to develop space for linking between each area, such as corridors or extra rooms. This makes it possible that the private area is linked to each other, at the same time it has its own distinctiveness according to its usage.
Won, Deok Hee;Han, Taek Hee;Park, Woo Sun;Park, Jong Sub;Kang, Young Jong
Journal of the Korea Academia-Industrial cooperation Society
/
v.14
no.2
/
pp.927-935
/
2013
Recently, bridge structures has progressed the researches about seismic performance by occurrence of earthquake increased compared with the past. In the substructure of bridge, confining transverse reinforcement has arranged in plastic hinge region to resist the lateral load which increased the lateral confining effect. Columns are increased the seismic performance through secure of the stiffness and ductility The design specification for arrangement of confining transverse reinforcement same specification of domestic and international that suggested to solid reinforced concrete column(RC). This design specification have limits for Internally Confined Hollow RC(ICH RC) column because of different the component and performance characteristics of column. In this paper suggested the modified equation for economics and rational design through investigation of displacement ductility when applied the existing specification at the steel composite hollow RC column.
Effects of soil moisture and temperature on the degradation rate of imazamethabenz were studied in two soils, a Declo sandy loam soil with 1.5% organic matter and pH of 8.0, and a Pancheri silt loam soil with 2.1% organic matter and pH of 7.7. Soils were incubated for 12 weeks under controlled conditions. Treatments were a factorial arrangements with combinations of three soil moistures (45, 75, 100% of field capacity) and two soil temperatures (20, 30C). Imazamethabenz degradation followed first-order kinetics for all soil moisture-soil temperature combinations. Imazamethabenz degradation rate was proportional to increase of soil moisture and temperature. Soil moisture effect on imazamethabenz degradation was greater when soil moisture was increased from 45 to 75% of field capacity (half-life decreased 2.6 fold) than when moisture increased from 75 to 100% of field capacity (half-life decreased 1.2 fold). Imazamethabenz degradation occurred more rapidly in the Pancheri silt loam than the Declo sandy loam soil. Formation of imazamethabenz acid from imazamethabenz followed a quadratic trend for most soil-moisture-soil temperature combinations. Imazamethabenz acid formation initially increased at earlier stages, but later gradually decreased. In most cases, increasing soil moisture and temperature appeared to accelerate it's acid breakdown to other metabolites.
Journal of the Korean Society of Marine Environment & Safety
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v.15
no.1
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pp.17-24
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2009
This study was conducted to find out the effects of relationship between ocean-meteorological factors and snowfall at Incheon, Gunsan and Mokpo when cold and dry continental air mass passed through the West Sea of Korea in winter. Mean snowfall from December to February showed the order of Gunsan (12.7 cm), Mokpo (9.0 cm) and Incheon (7.8 cm). In particular, the snowfall in the three regions showed the regional difference in December and February. It was well consistent with the extension of continental high. Extension of continental high can cause effect on snowfall at the west regions (Inchoen, Gunsan, Mokpo) of the Korean Peninsula. The continental high extended from the southern China to western coastal region of the Korean Peninsula in December, it extended from the northern China to central area of the Korean Peninsula in January. It also extended from the north side of China through Bohai Sea and Yodong Peninsula to central area of the Korean Peninsula in February. Therefore, more snowfall recorded in Incheon is higher in February than December whereas Gunsan and Mokpo is the opposite. The heavy snowfall at the three regions was caused by loss of the heat from the ocean to air when the heat loss was higher than 100 $W/m^2$. The heavy snowfall was also observed when the arrangement of continental high atmospheric pressure and low pressure was high at the west and low at the east, which formed a front in West and when the wind blow from the North or North West at the speed of 4${\sim}$8 m/sec.
Journal of the Korean Society for Marine Environment & Energy
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v.18
no.4
/
pp.274-281
/
2015
Thermal and nuclear power plants on shore commonly use the sea water for cooling facility. Discharged cooling water has the high kinematic energy potential due to amount of water flux. Numerical analysis was made to find the suitable combinations between the arrangement of tidal turbines and the overall dimensions of the discharged channel. Several parameters such as the turbine diameter to inlet size, and the axial distance to turbine size were investigated. Power coefficients for various test conditions were also compared to see the effect of inlet configurations such as single inlet and dual inlet. For the single inlet, the mean power coefficient appeared to be gradually decreased with increasing distance, and the maximum power was obtained when the turbine diameter was same as the inlet diameter. For the dual inlet, the tendency was similar so that the better result when the turbine diameter was same as the inlet diameter. It is expected that the present methodology can be extensively utilized to harness the high kinetic energy flow of the discharge channel of power plant.
Kim, Dong-Hoon;Kim, Myo-Kynng;Lee, Hoi-Chang;Ko, Duck-Sung;Park, Won-Il;Kwon, Hynck-Chan;Lee, Ho-Joon
Clinical and Experimental Reproductive Medicine
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v.28
no.2
/
pp.147-153
/
2001
Objective: The present study was performed to investigate the efficiency of partial laser assisted hatching (p-LAH; lased 1/2 ZP width from ZP edge) on hatching of mouse blastocysts. Methods: We used non-contact $1.48{\mu}m$ diode laser (MTM, Switzland) to create a precise hole on zona pellucida. 2-cell embryos were collected from the mouse (ICR) oviduct at 48 hours after hCG administration. Collected 2-cell embryos were cultured in the P-1 medium supplemented with 0.4% BSA. For experiments, embryos at 8-cell stage were used after $20{\sim}22$ hours in culture. After conventional (c-LAH) or partial laser assisted hatching, the embryos were further cultured in P-1 medium supplemented with 0.4% BSA for 3 days. To compare efficiency of complete and partial laser assisted hatching, hatching rate, hatching time and blastocyst diameter and zona pellucida thickness at hatching time were investigated. Embryos were examined every 12 hours. Blastocyst diameter and zona pellucida thickness at hatching time were measured with an ocular micrometer. Results: Hatching rates of p-LAH group (84.2%) was significantly higher than that of control group (39.3%), but there was no difference between the p-LAH (84.2%) and c-LAH (91.2%). p-LAH group was hatched 12 hours earlier than control group, but hatched 12 hours later than c-LAH group. The diameter of blastocyst at hatching time of p-LAH group ($113.1{\pm}6.4{\mu}m$) was smaller than that of control group ($122.2{\pm}5.0{\mu}m$), but larger than that of c-LAH group ($102.2{\pm}2.7{\mu}m$). Zona pellucida thickness at hatching time of p-LAH group ($6.4{\pm}0.9{\mu}m$) was thicker than that of control group ($4.5{\pm}1.5{\mu}m$), but thinner than that of c-LAH group ($10.0{\pm}0.8{\mu}m$). Conclusion: These results suggest that p-LAH may maintains the cell arrangement of early embryos to ensure successful development and prevent precocious hatching of blastocyst when compare to c-LAH and conventional (acidic tyrode) AH. Thus, p-LAH may provide a valuable and effective AH technique for human ART program.
A study was conducted to determine the effects of restricted feeding and feed form on the growth performance, characteristics of carcass and digestive tract, and days to first egg of Japanese quail (JQ, Coturnix coturnix japonica). A total of 240 oneweek-old JQ chicks were allocated randomly into 4 experimental groups that consisted of 3 replicates according to a 2${\times}$2 factorial arrangement for two feeding methods (ad libitum, AF and restricted feeding, RF) and two diet forms (mash, MD and crumble, CD). The JQ chicks were placed in a room with floor battery brooders and fed a commercial starter diet from 7 to 14 d of age. According to the experimental design, four treatments (1: ad libitum MD, 2: restricted MD, 3: ad libitum CD, and 4: restricted CD) were applied. Feed restriction was applied by 30% reduction of ad libitum feed intake for both MD and CD from 15 to 28 d of age. All birds were fed ad libitum with treatment diets from 29 d of age until the first laid egg seen (45 d of age). The commercial starter diet, MD and CD were in the same nutrient content (240 g crude protein with 13.4 MJ ME per kg diet). The body weight and overall feed conversion ratio (g feed/g gain) were higher (p<0.05) for the AF quails than the RF at 42 d of age. Carcass weights, dressing percentage and percentage yields of breast and back were similar for AF and RF groups at 42 d of age. The RF delayed (p<0.05) onset of egg production 2 days compared to the AF. Quail fed with the CD showed higher value (p<0.05) for carcass weight and dressing percentage at 42 d of age compared to birds fed with the MD. The interaction effect of feeding method${\times}$feed form on any of the studied parameters was not significant. The results suggest that feed restriction as in the present study can achieve a better feed conversion without reduction in carcass weight, and a significant benefit of feeding the crumble diet over the mash diet was obtained in terms of carcass weight in the JQ.
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