• International Journal of Stem Cells
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• v.16 no.3
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• pp.356-362
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• 2023

Glutathione (GSH) is a chief cellular antioxidant, affecting stem cell functions. The cellular GSH level is dynamically altered by the redox buffering system and transcription factors, including NRF2. Additionally, GSH is differentially regulated in each organelle. We previously reported a protocol for monitoring the real-time GSH levels in live stem cells using the reversible GSH sensor FreSHtracer. However, GSH-based stem cell analysis needs be comprehensive and organelle-specific. Hence, in this study, we demonstrate a detailed protocol to measure the GSH regeneration capacity (GRC) in living stem cells by measuring the intensities of the FreSHtracer and the mitochondrial GSH sensor MitoFreSHtracer using a high-content screening confocal microscope. This protocol typically analyses the GRC in approximately 4 h following the seeding of the cells onto plates. This protocol is simple and quantitative. With some minor modifications, it can be employed flexibly to measure the GRC for the whole-cell area or just the mitochondria in all adherent mammalian stem cells.

• Journal of Biosystems Engineering
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• v.15 no.1
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• pp.1-13
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• 1990

Aerodynamic property is the most important factor in designing the pneumatic separator and handling equipment for grains and seeds. Particularly the correct information about the terminal velocities of the corresponding grains and seeds is indispensible. However, a few studies with relation to the terminal velocities of grains and seeds were conducted in this country, even though the terminal velocities of the domestic grains and seeds are required to design those equiments which can be used for the domestic grains and seeds having specific aerodynamic properties. In this study, the terminal velocities for four varieties of varley and six varieties of paddy were investigated by means of two different methods, the suspension method and the drop method in an upward current of air. For measuring the terminal velocities, the vertical wind tunnel which had been examined about the uniform air flow in the previous study was used. In addition, the effect of the size of grains and the moisture content of grain kernel on the terminal velocity was examined. The following conclusions were derived from the study : 1. The different terminal velocities of grains are resulted from the different measuring methods. The terminal velocity measured by the drop method is smaller than that by the suspension method. It is considered that the difference in the terminal velocities is caused by the difference in the projection area of grain which is faced to the air stream. 2. The terminal velocity of grain increases as the size and the moisture content of the kernel increase. 3. The linear regression equations for the terminal velocities of grains were derived in terms of the moisture content of grains by the variety of grains and the measuring method. Also, the linear regression equations for the terminal velocity, based on the weighted size of grains, were derived in terms of the moisture contents of granis.

• Restorative Dentistry and Endodontics
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• v.42 no.4
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• pp.273-281
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• 2017

Objectives: The aims of this study were to quantify tug-back by measuring the pulling force and investigate the correlation of clinical tug-back pulling force with in vitro gutta-percha (GP) cone adaptation score using micro-computed tomography (${\mu}CT$). Materials and Methods: Twenty-eight roots from human single-rooted teeth were divided into 2 groups. In the ProTaper Next (PTN) group, root canals were prepared with PTN, and in the ProFile (PF) group, root canals were prepared using PF (n = 14). The degree of tug-back was scored after selecting taper-matched GP cones. A novel method using a spring balance was designed to quantify the tug-back by measuring the pulling force. The correlation between tug-back scores, pulling force, and percentage of the gutta-percha occupied area (pGPOA) within apical 3 mm was investigated using ${\mu}CT$. The data were analyzed using Pearson's correlation analysis, one-way analysis of variance (ANOVA) and Tukey's test. Results: Specimens with a strong tug-back had a mean pulling force of 1.24 N (range, 0.15-1.70 N). This study showed a positive correlation between tug-back score, pulling force, and pGPOA. However, there was no significant difference in these factors between the PTN and PF groups. Regardless of the groups, pGPOA and pulling force were significantly higher in the specimens with a higher tug-back score (p < 0.05). Conclusions: The degree of subjective tug-back was a definitive determinant for master cone adaptation in the root canal. The use of the tug-back scoring system and pulling force allows the interpretation of subjective tug-back in a more objective and quantitative manner.

• Journal of the Korean Chemical Society
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• v.50 no.5
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• pp.404-414
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• 2006

this study we analyzed and improved an experiment measuring chemical reaction rates introduced in the high school science textbooks through an understanding of the phenomena observed in carrying out the experiment. For this purpose, the contents of textbooks related to the experiment were analyzed, and the problems observed in carrying out the experiment were addressed through experimental analysis. When the experiment was carried out by the method of aquatic transposition presented in textbooks, the observed volume change of H2 gas was delayed and chemical reaction rate was increased in the early stage of reaction period. To resolve these problems, an improved method for measuring the reaction rates was suggested. In the improved experiment the reaction rate was measured to be constant on time, which was interpreted in terms of the concentration of H+ and the surface area of magnesium.

• Journal of Biomedical Engineering Research
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• v.35 no.6
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• pp.234-241
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• 2014

This study was designed to investigate the effects of stimulation intensity and inter-electrode distance on the parameters of the measured sensory nerve signal. 30 healthy subjects participated in this study. Sensory nerve signals were elicited by four different pulse amplitudes, i.e., 3, 6, 9, 12 mA, with the pulse width fixed at $500{\mu}s$. The sensory nerve signals elicited by the four different pulse amplitudes were measured by four different inter-electrode distances (20, 30, 40, and 50 mm). We extracted four parameters (pulse amplitude, pulse width, pulse area, and latency time from stimulation) from the sensory nerve signals. The measured pulse amplitude and pulse width were increased when the measuring inter-electrode distance was increased while the stimulating pulse amplitude was fixed. The measured pulse amplitude was saturated with the stimulating pulse amplitudes of over 6 mA while measuring inter-electrode distance. Under the same condition, measured pulse width was increased, and sensory nerve signal was initiated early. Sensory nerve signals, specially those of pulse amplitude, were distorted by a differential amplification method that commonly measures the human body signal. The experimental results indicate that the differential amplification method is required to be replaced when measuring nerve signals. Our observations suggested that the hyperpolarization of the action potential of the sensory nerve signal for preventing distortion could be used to clarify the correlation between the parameters of the sensory nerve signals and quantification of sensations.

• Journal of Advanced Navigation Technology
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• v.18 no.3
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• pp.201-208
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• 2014

Due to diversification of ships and limited space of pier, when ships come alongside the shallow water or narrow pier, sea area with small mooring facilities and floating matter is frequently applied. Through these, coming alongside the various space is capable and cost effectiveness is enhanced. However, when ships, applying small mooring facilities and floating matter, come alongside, there can be some impulse by waves between the floating things and ships which possibly leads to mass disaster. Therefore, there should be forecasts and analysis of the movement caused by waves. On this study, we develop measuring system for movement analysis of mooring and floating matters which provides base data with movement traits by measuring 3-D exercise information and acceleration at mokpo maritime university marina facility. Also, the composition and principles of the developed system is explained.

• Education of Primary School Mathematics
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• v.19 no.2
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• pp.113-125
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• 2016

The concept and measuring activities of the height of figures are essential to find the areas or volumes of the corresponding figures. For plane figures, the height of a triangle is defined to be the line segment from a vertex that is perpendicular to the opposite side of the triangle, whereas the height of a parallelogram(trapezoid) is defined to be the distance between two parallel sides. For the solid figures, the height of a prism is defined to be the distance of two parallel bases, whereas the height of a pyramid is defined to be the perpendicular distance from the apex to the base. In addition, the height of a cone is defined to be the length of the line segment from the apex that is perpendicular to the base and the height of a cylinder is defined to be the length of the line segment that is perpendicular to two parallel bases. In this study, we discuss some pedagogical problems on the concepts and measuring activities of the height of figures to provide alternative activities and suggest their educational implications from a teaching and learning point of view.

• Journal of the Korea Society of Computer and Information
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• v.24 no.12
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• pp.127-134
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• 2019

In this paper, we designed and implemented a system for automatically measuring pig growth rate using image. Pig breeding is a very important factor because the breeding rate of pigs is directly linked to the rancher's economic interests in order to raise them in livestock farms. Therefore, there is a demand for a method for easily measuring the growth rate of each pig. As a method for this, an image analysis algorithm for measuring the growth rate of pigs is presented by taking a plan view of the pig. In addition, the proposed algorithm was implemented to measure the surface area of swine and to express it as the rate of gain. The proposed method in this paper makes it possible to easily measure the individual growth rate of pigs to meet the needs of farmers. In the future, we will improve the algorithm to calculate the volume so that the same value as the actual measurement can be obtained.

• The Korean Journal of Community Living Science
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• v.14 no.3
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• pp.89-98
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• 2003

To assess the actual status of businesses selling fermented soybean products, this study surveyed 130 Farmhouse style small size soybean sauce processing sites supported by the Rural Development Administration nationwide. The sex composition of the businesses representatives of 2% male and 98% female showed that a farmhouse soybean processing business is generally operated by rural women as a non-filming business. The percentage of co-worked sites was 71.2 and that of sites with permits was 39.2. The main products were meju, doenjang, and kanjang. Total production volume was found to be 523 tons, 256 tons, and 135 k$\ell$s, respectively. The number of years of experience of the manufacturers of the kanjang and doenjang was found to be 25.7 on average which shows that long-experienced, skilled manufacturers are participating in this project. The traditional measuring unit differed greatly depending on the region. This difference in the measuring unit of soybeans resulted in a large difference in the volume of doenjang and kanjang produced Per Province. For one kg of raw soybeans, the weight of Doenjang produced also varied from 2.14 kg in the Gyeongbuk area to 1.62 kg in Jeju. In the aspect of salt use, 1.75 $\ell$ (translated by volumetric unit 0.704 due) of salt pet kg of meju, in Jeju, was the highest in Korea. Government supervision would be beneficial in the areas of raw material products, standardization of measuring units and production methods, organization and operation of a conference for the different groups of producers, and in construction of a nationwide database.

• Journal of Magnetics
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• v.21 no.2
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• pp.298-302
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• 2016

In bone density examinations, a change in the measured BMD occurs owing to the differences between the measured areas. To address this problem, we aimed to develop a new auxiliary device that could be rotated by $15^{\circ}$ by fixing the ankle to the distal femur neck. Dual energy X-ray absorptiometry (DXA) of BMD examinations were performed once a year, but 10 patients were examined over three sessions to analyze the area for measuring the femur neck BMD. The goal of this test was to determine the device's reliability, and the results were expressed in terms of the standard deviation of measurements. After performing bone density measurements using the new auxiliary device on 10 normal patients, with three measurements for each patient, the obtained standard deviation was 0.03. The standard deviation of the measured BMD was 0.19 when using the currently existing auxiliary device, while the standard deviation of the measured BMD was 0.03 when using the new auxiliary device. By using the new auxiliary device, the standard deviation could be reduced by ~80%. Accurate rotation of the femur neck was possible in all examinations, and the standard deviation of BMD measurements could be reduced by up to 80% compared with the measurements performed using the currently existing auxiliary device. We hope that this advantageous new design can be used as a standard auxiliary device for measuring the femur neck BMD.