• Title/Summary/Keyword: apical size

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Elimination of SPFMV from Virus-infected Sweet Potato Plants through Apical Meristem Culture

  • Kim, Young-Seon;Jeong, Jae-Hun;Park, Jong-Suk;Eun, Jong-Seon
    • Plant Resources
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    • v.7 no.3
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    • pp.200-205
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    • 2004
  • Sweet potato infected with a viral disease (SPFMV) showed irregular chlorotic patterns, so called feathering associated with faint or distinct ring spots that have purple-pigmented borders. SPFMV was eliminated from sweet potato plants using meristem tip culture. MS medium supplemented with BAP (2mg/L) and NAA (0.05 mg/L) was used for shoot proliferation and 1/2 MS medium for rooting of the plants. Highest percentage of regenerated plants (60%) was obtained from the optimum size (0.3-0.5mm) meristem tips. Of these, 60% plants were found negative for SPFMV by RT-PCR. Virus detection by RT-PCR was found to be a reliable method. Meristem-tip culture to produce SPFMV-free quality sweet potato and virus detection by RT-PCR is an efficient, time saving and reliable method for production of SPFMV-free tissue culture raised plants.

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Traumatic Ventricular Septal Defect Secondary to Nonpenetrating Chest Trauma -A Case Report- (비관통성 외상에 의한 심실중격결손증 -수술치험 1례-)

  • 홍기표
    • Journal of Chest Surgery
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    • v.27 no.2
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    • pp.161-165
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    • 1994
  • We have experienced a case of ventricular septal defect due to blunt chest trauma. A 22 year old male patient was admitted due to chest pain after Motor cycle accident on July 1st,1993. On 5th hospital day, sudden onset of dyspnea was noted and auscultation represented newly developed systolic murmur. A cardiac catheterization and Left ventriculogram revealed ruptured septum at the apical portion. Because there was open wound on anterior chest wall and congestive heart failure was medically controlled, the patient was discharged for elective operation. He was readmitted on August 14th, 1993.At operation, ventricular septal defect was found in apico-posterior muscular septal area, about 2.0 x 1.5 cm in size. The defect was repaired by double velour patch with interrupted suture and ventriculotomy was closed with Teflon felt. The patient`s postoperative course was uneventful and discharged 10 days postoperatively without complication. The patient have been followed up~ for 2 months. He is on functional class I with small amount of residual shunt at the ventricular septum.

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First Report on Gray Blight of Tea Plant Caused by Pestalotiopsis theae in Korea

  • Shin, Gil-Ho;Park, Hyoung-Koog;Hur, Jae-Seoun;Koh, Young-Jin
    • The Plant Pathology Journal
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    • v.15 no.5
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    • pp.308-310
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    • 1999
  • A fungus associated with gray blight on tea plant (Camellia sinensis) was identifed as Pestalotiopsis theae based on the mycological characteristics. Mycelial growth on potato dextrose agar and size and shape of conidia of P. theae were similar to those of P. longiseta, but P. theae was different from P. longiseta in the color of three median cells and the number of apical appendages. Artificial inoculation of conidial suspension or mycelial mats on the wounded leaves and shoots of healthy plants induced the same disease, respectively. The Korean native variety was relatively. The Korean native variety was relatively more resistat to P. theae than a Japanese variety‘Yabukita’which has bee recently introduced and planted in large areas of southern parts of Korea. Here, we report the report the first record of gary blight caused by P. theae on tea plant in Korea.

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Riffle beetle genus Leptelmis (Coleoptera Elmidae) in Korea with descriptions of two new species

  • Jung, Sang-Woo;Bae, Yeon-Jae
    • Animal cells and systems
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    • v.16 no.3
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    • pp.254-259
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    • 2012
  • The riffle beetle genus Leptelmis is newly recorded in the Korean fauna with descriptions of two new species, L. coreana, n. sp. and L. ochra, n. sp. Dorsal habitus and line-drawings of diagnostic characters are provided. The adults of L. coreana and L. ochra are similar to those of L. gracilis Sharp or L. parallela Nomura in general appearance, but can be distinguished by the absence of apical spurs on the middle and hind tibiae, light brown body color, smaller body size, and the shape of aedeagus apex. Leptelmis coreana can be distinguished from L. ochra by the absence of hindwings, lack of prominent humeri and wider elytra in posterior portion. Adults of both species occur in lowland streams and large rivers, but L. ochra were encountered relatively infrequently.

Comparative Study on the Structure of Vascular Cambium in Some Salicaceae (수종의 버드나무과 유관속 형성층의 구조에 관한 비교연구)

    • Korean Journal of Plant Resources
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    • v.11 no.3
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    • pp.326-334
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    • 1998
  • Vascular cambium in 16 species of Salicaceae is nonstoried in all of them and is made up of fusiform and ray initials. The growth of cambial girth occurred by pseudotransverse division of fusiform initials and subsequent apical intrusive growth of daughter initials. The measurements of fusiform initials in tangerntial sections have revealed that the average length varies from 252.2-437.1${\mu}{\textrm}{m}$ in investigated species, with the maximum being in Populus euramericana and the minimum in Salix purprea var. Multinervis. The length of fusiform initials depends on the length of the tapering end wall , therefore, the longer the length of the tapering end wall, therefore, the longer the length of the taperding end wall, the longer the length of fusiform initials. Ray initials have their own size, magnitude, and frequency of occrrence in each species. The relative proportion of ray initials fall about 15.0-24.5% in Salicaceae. Shrubby species of Salix have a lower ratio of ray initials than the species which possess wider trunks. New ray initials are originated from cut off the side or the end of fusiform initials and central partition of a fusiform initials.

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New record of the cold freshwater dinoflagellate Palatinus apiculatus (Dinophyceae) from the Paldang Reservoir, Korea

  • Kim, Taehee;Ki, Jang-Seu
    • Journal of Species Research
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    • v.11 no.3
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    • pp.162-168
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    • 2022
  • Compared to marine dinoflagellates, freshwater species are rarely recorded in Korea. In the present study, we isolated a freshwater dinoflagellate, Palatinus, from the Paldang Reservoir, Korea, in December 2021. The overall cell shape was ovoid, and the cell size was 34.3 ㎛ in length (25.8-39.5 ㎛) and 28.4 ㎛ in width (21.5-34 ㎛). An eyespot was usually observed near the sulcal region. The Kofoidian plate formula of the species was determined to be 4', 2a, 7", 6c, 5s, 5''', and 2''''. Apical pore complex was not observed. However, variations in the cingular plate caused by the fusion of 3C and 4C were observed. Analyses of 28S rDNA sequences revealed that the unidentified species is 100% similar to Palatinus apiculatus, and clustered together in the same lineage in the phylogenetic tree (100% bootstrap value). Our findings confirmed that the isolated dinoflagellate is Palatinus apiculatus, which was discovered for the first time in Korean freshwaters.

Review of the genus Eopompilus Gussakovskij, 1932 (Hymenoptera: Pompilidae) in South Korea

  • Kim, Jeong-Kyu
    • Journal of Species Research
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    • v.11 no.3
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    • pp.194-201
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    • 2022
  • Species of the genus Eopompilus Gussakovskij, 1932 from South Korea are reviewed. Three species are confirmed: E. internalis, E. luteus, and E. minor. Eopompilus luteus is recorded for the first time in Korea, and is easily separated from its congers by markedly extensive coloration and comparatively large body size in both sexes. Females are characterized by yellowish wing, light brown stigma and veins, lacking of subapical or apical dark fascia on forewing. Males are characterized by serrate antenna formed by submesal convex on each flagellomeres 2-11, symmetrical claws of fore tarsus, hypopygium widened basally and forming a rhomboid plate, lacking of dark fasciae in forewing. The specimen identified as Cryptocheilus nicevilli Bingham from Korea by Kim (1970) is examined and is reclassified as conspecific with E. luteus. A key is presented of the three species complemented by digital images and diagnosis.

Buckling resistance, torque, and force generation during retreatment with D-RaCe, HyFlex Remover, and Mtwo retreatment files

  • Yoojin Kim ;Seok Woo Chang;Soram Oh
    • Restorative Dentistry and Endodontics
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    • v.48 no.1
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    • pp.10.1-10.9
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    • 2023
  • Objectives: This study compared the buckling resistance of 3 nickel-titanium (NiTi) retreatment file systems and the torque/force generated during retreatment. Materials and Methods: The buckling resistance was compared among the D-RaCe (DR2), HyFlex Remover, and Mtwo R25/05 retreatment systems. J-shaped canals within resin blocks were prepared with ProTaper NEXT X3 and obturated by the single-cone technique with AH Plus. After 4 weeks, 4 mm of gutta-percha in the coronal aspect was removed with Gates-Glidden drills. Retreatment was then performed using DR1 (size 30, 10% taper) followed by DR2 (size 25, 4% taper), HyFlex Remover (size 30, 7% taper), or Mtrwo R25/05 (size 25, 5% taper) (15 specimens in each group). Further apical preparation was performed with WaveOne Gold Primary. The clockwise torque and upward force generated during retreatment were recorded. After retreatment, resin blocks were examined using stereomicroscopy, and the percentage of residual filling material in the canal area was calculated. Data were analyzed using 1-way analysis of variance with the Tukey test. Results: The HyFlex Remover files exhibited the greatest buckling resistance (p < 0.05), followed by the Mtwo R25/05. The HyFlex Remover and Mtwo R25/05 files generated the highest maximum clockwise torque and upward force, respectively (p < 0.05). The DR1 and DR2 files generated the least upward force and torque (p < 0.05). The percentage of residual filling material after retreatment was not significantly different between file systems (p > 0.05). Conclusions: NiTi retreatment instruments with higher buckling resistance generated greater clockwise torque and upward force.

TRANSPORTATION OF CURVED CANAL AFTER CANAL ENLARGEMENT ACCORDING TO FILING INSTRUMENTS (만곡근관의 확대시 기구에 따른 형태변화에 관한 연구)

  • Lee, Seok-Jong;Shin, Young-Guen;Hwang, Ho-Keel
    • Restorative Dentistry and Endodontics
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    • v.24 no.3
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    • pp.503-510
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    • 1999
  • The purpose of this study was to evaluate the amount of transportation of original canal, zip formation, permanent deformation and fracture of instruments after canal enlargement. In this study, the 60 resin blocks that have curved canals were randomly divided into 3 experimental groups with 20 teeth each according to instrument types and filling methods for canal enlargement. The curved canals of each experimental groups were enlarged to No 40 ISO size with the K-flexo stainless steel file (Group 1), Engine-driven Ni-Ti Profile new series(Group 2) and Engine-driven Ni-Ti Quantec 2000 series(Group 3) according to the manufacturer's recommendation. Pre- and postoperative X-rays were taken at same position and the films were scanned and the canal images were traced to determine the canal curvature according to the method of Schneider. The amount of reduction in canal curvature were calculated between pre- and postoperative X-rays. In addition to zip formation, permanent deformation and fracture of instruments were examined after canal enlargement. The results were as follows : 1. All experimental groups showed some loss of canal curvature after instrumentation. There was a significant change in curvature between before and after instrumentation in each group(p<0.001). 2. Engine-driven Ni-Ti instrumentations resulted in an average loss of curvature of 2.36 degrees for Profile new series, 3.43 degrees for Quantec series, and hand instrumentation showed an average loss of curvature of 6.48 degrees for K-flexo file. There was a statistical significant difference between hand instrumentation and engine-driven Ni-Ti instrumentations(p<0.05). But there was no statistical difference between Profile new series and Quantec series. 3. There were many apical zip formations in group 1(Hand instrumentation). But there were no apical zip formations in group 2,3(Engine-driven Ni-Ti instrumentation). 4. The instrument deformation occured 9 cases in group 1(K-flexo file), 2 cases in group 2(Profile new series) and 3 cases in group 3(Quantec) after instrumentation. And the instrument fracture occured 1 case in each group. The results showed that the engine-driven Ni-Ti instruments, if we use carefully according to manufacturer's recommendations, can be use effectively for instrumenting the curved root canals in case of the MAF was over size 40.

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Effect of Particle Size of Zinc Oxides on Cytotoxicity and Cell Permeability in Caco-2 Cells

  • Chang, Hyun-Joo;Choi, Sung-Wook;Ko, Sang-Hoon;Chun, Hyang-Sook
    • Preventive Nutrition and Food Science
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    • v.16 no.2
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    • pp.174-178
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    • 2011
  • The cell permeability and cytotoxic effects of different-sized zinc oxide (ZnO) particles were investigated using a human colorectal adenocarcinoma cell line called Caco-2. Morphological observation by scanning electron microscopy revealed that three zinc oxides with different mean particle sizes (ZnO-1, 20 nm; ZnO-2, 90~200 nm; ZnO-3, $1\sim5\;{\mu}m$) tended to aggregate, particularly in the case of ZnO-1. When cytotoxicities of all three sizes of zinc oxide particles were measured at concentration ranges of $1\sim1000\;{\mu}g$/mL, significant decreases in cell viability were observed at concentrations of $50\;{\mu}g$/mL and higher. Among the three zinc oxides, ZnO-1 showed the lowest viability at $50\;{\mu}g$/mL in Caco-2 cells, followed by ZnO-2 and ZnO-3. The permeate concentration of ZnO-1 from the apical to the basolateral side in the Caco-2 model system after four hours was about three-fold higher than that of either ZnO-2 or ZnO-3. These results demonstrated that ZnO-1, with a 20 nm mean particle size, had poorer viability and better permeability in Caco-2 cells than ZnO-2 and ZnO-3.