• ALGAE
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• 제37권1호
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• pp.75-84
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• 2022

Intercellular nutrient and signal transduction are essential to sustaining multicellular organisms and maximizing the benefits of multicellularity. It has long been believed that red algal intercellular transport of macromolecules is prevented by the protein-rich pit plug within pit-connections, the only physical connection between cells. Fluorescein isothiocyanate-dextran and recombinant green fluorescence protein (rGFP) of various molecular sizes were injected into vegetative cells of Griffithsia monilis using a micromanipulator, and intercellular transport of the fluorescent probes was examined. Pit-connections were found to provide intercellular transport of tracers at rates comparable to plasmodesmata in other organisms. The time necessary for the transport to an adjacent cell was dependent on the molecular size and the direction of the transport. Fluorescent dextran of 3 kDa was transported to adjacent cells in 1-2 h after injection and migrated to all cells of the filament within 24 h, but fluorescent dextran of 10-20 kDa took 24 h to transfer to neighboring cells. The migration occurred faster towards adjacent reproductive cells and to apical cells than basally. Fluorescent tracers above 40 kDa and rGFP was not transported to neighboring cells, but accumulated near the pit plug. Our results suggest that pit-connections are conduit for macromolecules between neighboring cells and that these size-specific conduits allow intercellular communication between the vegetative cells of red algae.

• Restorative Dentistry and Endodontics
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• 제48권2호
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• pp.21.1-21.15
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• 2023

Objectives: This study evaluated the effects of Biodentine (BD), Bio-C Repair (BCR), and mineral trioxide aggregate (MTA) plug on the fracture resistance of simulated immature teeth with replacement root resorption (RRR) and in vitro-induced osteoclastogenesis. Materials and Methods: Sixty bovine incisors simulating immature teeth and RRR were divided into 5 groups: BD and BCR groups, with samples completely filled with the respective materials; MTA group, which utilized a 3-mm apical MTA plug; RRR group, which received no root canal filling; and normal periodontal ligament (PL) group, which had no RRR and no root canal filling. All the teeth underwent cycling loading, and compression strength testing was performed using a universal testing machine. RAW 264.7 macrophages were treated with 1:16 extracts of BD, BCR, and MTA containing receptor activator of nuclear factor-kappa B ligand (RANKL) for 5 days. RANKL-induced osteoclast differentiation was assessed by staining with tartrate-resistant acid phosphatase. The fracture load and osteoclast number were analyzed using 1-way ANOVA and Tukey's test (α = 0.05). Results: No significant difference in fracture resistance was observed among the groups (p > 0.05). All materials similarly inhibited osteoclastogenesis (p > 0.05), except for BCR, which led to a lower percentage of osteoclasts than did MTA (p < 0.0001). Conclusions: The treatment options for non-vital immature teeth with RRR did not strengthen the teeth and promoted a similar resistance to fractures in all cases. BD, MTA, and BCR showed inhibitory effects on osteoclast differentiation, with BCR yielding improved results compared to the other materials.

• 대한소아치과학회지
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• 제39권4호
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• pp.397-403
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• 2012

미성숙 영구치는 치아우식증, 외상, 비정상적인 치아 형태로 인한 파절 등에 의하여 치수 생활력을 상실할 수 있다. 이때 이용되는 치료법이 치근단 형성술로서 실활치의 치근단 치수 부위에 광화된 조직의 형성을 통하여 치근단 폐쇄를 유도한다. 현재 수산화칼슘 치근단 형성술이 가장 많이 이용되는데 근관 내에 수산화칼슘을 장기간 적용할 경우 치근단 경조직 형성을 유도할 수 있지만, 강한 알칼리성으로 인하여 치근 약화를 야기하여 치아 파절 위험성이 높아진다. 그러므로 근관 충전 시 치아보강을 위한 수복이 고려되어야 한다. 본 증례는 생활력을 상실한 미성숙 영구치에서 장기간의 수산화칼슘 치근단 형성술 후 치아 보강을 위하여 MTA plug, 섬유강화형 포스트 그리고 복합 레진을 이용한 근관 충전을 시도하였기에 보고하는 바이다.

• Restorative Dentistry and Endodontics
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• 제36권6호
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• pp.510-514
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• 2011

Mineral trioxide aggregate (MTA)는 치근 천공을 수복하기 위한 생체적합성이 우수한 재료로 소개되었으며 현재 근관 치료의 다양한 분야에서 사용되고 있다. MTA는 전통적인 수복재료와는 달리 수분이 있는 환경에서 경화되는 특징이 있다. 임상에서 흔치는 않지만 경화되지 않은 MTA가 관찰되는 경우가 있으나 이의 원인은 불명확하며 관련된 연구도 많지 않다. 본 증례는 정 방향 MTA 충전 6년 후 MTA가 소실(wash-out)된 환자에 대한 증례로서 그 원인에 대해 고찰해보고자 한다.