• 한국자원식물학회지
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• 제19권2호
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• pp.360-364
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• 2006

도깨비고비와 참쇠고비의 포자발아에서 얻은 전엽체를 균질화하여 배양함으로써 전엽체 개체발생과 포자체 형성과정을 연구하였다. 배양 2주일이 지나자 균질화한 전엽체 세포들이 일차원의 필라멘트 형태를 형성하였고, 4주일째에는 다수의 가지를 뻗은 형태의 전엽체가 출현하였다. 배양 6주일째에 apical notch가 발달된 전엽체가 형성되었는데. 중심부에는 분열조직이 발달되었다. 8주의 배양기간이 지나자 전엽체의 중심부에서 장란기의 형성 없이 무수정생식에 의한 bud가 관찰되었다. 10주일이 지나자 무수정생식에 의한 bud는 포자체로 발달되었는데, flow cytometric 분석 결과 도깨비고비와 참쇠고비 모두에서 전엽체와 포자체는 동일한 ploidy level을 지닌 것으로 확인되었다. 이는 도깨비고비뿐만 아니라 참쇠고비 또한 무수정생식을 하는 양치식물임을 증명하는 결과로 생각된다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2021년도 춘계학술대회
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• pp.23-23
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• 2021

Apple (Malus×domestica Borkh.; Rosaceae) is an important fruit crop grown mainly in temperate regions of the world. Tissue culture in vitro is a biotechnological technique that has been used to genetically improve cultivars (scions) and rootstocks. This could be important in the production of genetically uniform scions and rootstocks for commercial apple production. In nurseries, apple plants are produced by grafting scions onto rootstocks. The Cornell-Geneva (Geneva® series) breeding program has bred several dwarf rootstocks that are resistant to diseases and pests and are also cold hardy. This study was conducted to determine the optimal medium strength to improve sprouting shoot rate of apical meristem of the apple rootstocks of fire blight resistance. The apple rootstocks apical meristem at size (0.2 mm to 0.3 mm) with axillary buds were cultured on the MS(Murashige & Skoog) medium supplemented with plant growth regulators. The sprouting ratio and growth characteristics was evaluated after eight weeks in vitro culture. The highest rate of bud differentiation and shoot formation were 23.8% and 55.6%, respectively. After 6 weeks, shoots were regenerated from apical meristem, and their growth characteristics was significantly varied on the respective basal medium with different plant growth regulators. Our studies showed that the apple rootstocks the apple rootstocks of fire blight resistance plantlets could be successfully produced from apical meristem differentiated out of young twigs via organogenic regeneration.

• Journal of Plant Biotechnology
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• 제37권4호
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• pp.343-356
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• 2010

Plant micropropagation techniques include bud cultures using apical or axillary buds, organogenesis through callus culture or adventitious bud induction, and somatic embryogenesis. In Korea Forest Research Institute (KFRI), the first tissue culture trial in woody plant was initiated from the bud culture of hybrid poplars (Populus alba x P. glandulosa) in 1978. Since then several mass propagation techniques have developed from conifer and hardwood species, resulting in allowing practical application to Poplars, Birches and some oak species. In addition, useful micropropagation and genetic resources conservation techniques were established in some rare and endangered tree species including Abeliophyllum distichum. Among various in vitro propagation techniques, somatic embryogenesis is known to be the most efficient plant regeneration system. Since the first somatic embryo induction was reported in Tilia amurensis by KFRI in 1986, various protocols for direct or indirect somatic embryogenesis systems have developed in conifer and hardwood species including Larix leptolepis, Pinus rigida x P. taeda F1, Kalopanax septemlobus and Liliodendron tulipifera, etc. However, most of these technologies have been developed using juvenile tissues, i.e. immature zygotic embryos or mature embryos. Therefore it has been difficult to directly application to tree breeding program due to their unproven genetic background. Recently remarkable progresses and new approaches have been achieved in mature tree somatic embryogenesis. In this article we reviewed several micropropagation techniques, which have been mainly developed by KFRI and recent international progresses.

• Journal of Plant Biology
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• 제37권1호
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• pp.17-25
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• 1994

Two potato (Solanum tuberosum L.) cultivars were transformed by Agrobacterium tumefaciens harboring cauliflower mosaic virus (CaMV) 35S promoter and $\beta$-glucuronidase (GUS) gene. Expression patterns of the CaMV 35S promoter according to tissue types and developmental stages, and genetic stability of GUS gene were investigated in the clonal progenies of transgenic potatoes. Kanamycin-resistant shoot emerged from tuber disc after 4 weeks of culture, and root was induced 6 weeks after culture on the selection medium. Shooting frequency of cvs. Superior and Dejima were 43% and 27%, respectively. Mature transformants and their clonal progenies showed no phenotypical abnormality. GUS activity was expressed primarily at parenchymatous cells of phloem tissue around the vascular cambium in the stem and root, and higher activity was found at the apical meristem of shoot, root and adventious shoot bud. GUS activity was higher at tubers of young explants than at stored tubers. These facts indicate that expression level of the CaMV 35S promoter differed according to tissue types and developmental stages of the organs. The GUS gene was stably inherited to each clonal progeny and normally expressed.

• 한국자원식물학회지
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• 제21권5호
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• pp.362-367
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• 2008

할미꽃의 정단 분열조직배양에 의한 기내 효율적 미세번식에 미치는 식물생장조절물질의 효과를 조사하기 위해 2,4-D, NAA, kinetin, TDZ와 BA를 혼용처리한 MS배지에 정단분열조직을 배양하였다. 배지내 2,4-D와 kinetin, 2,4-D와 TDZ, NAA와 TDZ 혼용처리는 캘러스 유도에 효과적이지 못하였으나 NAA와 BA가 혼용 처리되었을 때 배발생적 또는 기관분화성 캘러스가 유도되었다. 특히 0.5mg/L NAA와 1.0mg/L BA 혼용처리된 MS 배지에서는 캘러스로부터 고빈도로(62%) 신초가 형성되어 캘러스 유도 및 신초형성에 적합하였다. 적합배지에 10% coconut water를 첨가하여 30일마다 계대배양 하므로 캘러스 증식률, 신초 분화율 및 신초 신장이 현저히 증가되었는데, 그 결과 절편체 당 평균 12.9개의 shoot bud를 분리할 수 있었다. 할미꽃 기내 유도된 식물체에서 뿌리는 쉽게 분화되지 못하였으나 9.0mg/L NAA 단용 또는 0.5mg/L NAA와 1.0mg/L BA 혼용처리된 MS배지에서 캘러스를 통해서만 분화되었다. 이들 뿌리는 동일 배지에서 캘러스유도 및 식물체 분화를 반복적으로 실시하는 시원체가 되기도 하였다.

• 식물조직배양학회지
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• 제21권5호
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• pp.277-280
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• 1994

생장점배양하여 얻어진 소수의 식물체를 기내 재배양하여 건전 우량마늘을 대량으로 증식시킬 수 있었다. MS 기본배지에 서당 8%, NAA 0.1 mg.L 첨가된 배지에 지엽을 뚫고 나온 직후의 주아 생장점을 5월 중순에 치상하면 shoot의 형성 없이 기내인경이 직접 형성되어 배양 기간 및 노력을 절약할 수 있었다. 또한 총포 내의 미숙주아 및 receptacle을 6월 상순경에 0.1 mg/L NAA+2mg/L BA 혹은 1 mg/L NAA + 10 mg/L BA가 첨가된 MS 기본배지에 배양하면 multiple shoot가 유도되었다. 특히 여러 화기 재료중 receptacle에서 평균 5.8개의 shoot가 분화되어 1개의 총포 내에서 50개 이상 분화되었다. 따라서 총포배양에 의한 식물체 생산은 우량마늘의 대량번식 체계의 한 수단으로 이용할 수 있었다.

• 화훼연구
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• 제17권2호
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• pp.101-105
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• 2009

본 연구는 화학적심제 dikegulac의 처리시기와 농도가 Rhododendron simsii 'California Sunset'의 분지 및 생육에 미치는 영향을 알아보고자 수행하였다. 6월에 dikegulac의 처리는 8월에 처리한것 보다 식물의 정아 발달을 지연시켰으나, 잎의 크기와 엽록소의 함량 및 분지수를 증가 시켰다. 특히, $1,500mg{\cdot}L^{-1}$의 처리가 가장 우수하였다.

• 식물조직배양학회지
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• 제26권4호
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• pp.271-274
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• 1999

아배양을 통한 물박달나무 성숙목의 기내증식은 DKW 배지에서 양호하였다. 이 배지는 다른 두 배지보다 줄기증식 및 줄기생장이 양호하여 물박달나무의 적정 배지로 생각되었다. DKW 배지에서 액아배양과 정아배양에는 큰 차이는 없었으나 증식에는 액아배양이, 생장에는 정아배양이 다소 양호하였다. 한편 1/2 MS 배지에서는 절편기부에 callus가 직경 1.0cm이상 지나치게 형성되어 줄기신장을 억제하였고, WPM에서는 줄기형태는 정상이나 생장이 저조하여 물박달나무의 아배양 배지로는 부적당하였다. 발근은 NAA 처리가 IBA 보다 효과적이었다. l/2 DKW 배지에 1.0 mg/L NAA 처리로 80%의 기내발근 되었으며 유도된 뿌리수도 많았다 얻어진 줄기는 직접 기외삽목도 가능하였는데, 이 같은 결과는 물박달나무 성숙목의 효율적인 기내번식이 가능함을 시사한다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2000년도 The 7th International Symposium
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• pp.114-115
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• 2000

Aralia elata is found in mountain areas all over Korean peninsula. Aralia elata is the scientific name for Japanese angelica tree. The tree belongs to the family Araliaceae, commonly known as ginseng family. Bud sprouts from apical shoot tip of the plants are rich in flavor and thus mainly used for both folk medicine and vegetable. The stalks with apical buds are gathered in the early spring and planted in sandy soil or water in the greenhouse. The sprouting buds are then collected and sold as fresh vegetable. Although the plants have been used for food, they have been cultivated in a very small scale. In spring, local farmers just go around mountain areas to search the trees and gather the stalks as much as they get and sell them to the market. No conservation efforts have been made to stop the exploitation or to save the dwindling population. We tried to provide local farmers with the plants that may be used as an alternative to stalks from wild populations. This will bel! p conserve the wild populations. However, it is hard to propagate them either by conventional cuttings or by seed germination in a short period of time. Mass propagation using tissue culture systems have shown a great promise with several woody plants. Recently we developed a mass propagation technique via somatic embryogenesis system using mature and/or juvenile explants for Aralia elata. Several factors affecting somatic embryogenesis system including SE(somatic embryo) induction, embryogenic callus proliferation, SE germination, plant regeneration and transplanting to field frill be presented. And some problems arising for the somatic embryogenesis system will be also discussed.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2000년도 제7차 국제 심포지움(생약자원개발에 관한연구) 및 추계정기 학술발표회 초록집
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• pp.16-17
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• 2000

Aralia elata is found in mountain areas all over Korean peninsula. Aralia elata is the scientific name for Japanese angelica tree. The tree belongs to the family Araliaceae, commonly known as ginseng family. Bud sprouts from apical shoot tip of the plants are rich in flavor and thus mainly used for both folk medicine and vegetable. The stalks with apical buds are gathered in the early spring and planted in sandy soil or water in the greenhouse. The sprouting buds are then collected and sold as fresh vegetable. Although the plants have been used for food, they have been cultivated in a very small scale. In spring, local farmers just go around mountain areas to search the trees and gather the stalks as much as they get and sell them to the market. No conservation efforts have been made to stop the exploitation or to save the dwindling population. We tried to provide local farmers with the plants that may be used as an alternative to stalks from wild populations. This will hel! p conserve the wild populations. However, it is hard to propagate them either by conventional cuttings or by seed germination in a short period of time. Mass propagation using tissue culture systems have shown a great promise with several woody plants. Recently we developed a mass propagation technique via somatic embryogenesis system using mature and/ or juvenile explants for Aralia elata. Several factors affecting somatic embryogenesis system including SE(somatic embryo) induction, embryogenic callus proliferation, SE germination, plant regeneration and transplanting to field will be presented. And some problems arising for the somatic embryogenesis system will be also discussed.lso discussed.