• Archives of Pharmacal Research
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• 제28권5호
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• pp.518-528
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• 2005

In the present study, the antioxidative and inhibitory activity of Zingiber officinale Rosc. Rhizomes-derived materials (on mushroom tyrosinase) were evaluated. The bioactive co mponents of Z. officinale rhizomes were characterized by spectroscopic analysis as zingerone and dehydrozingerone, which exhibited potent antioxidant and tyrosinase inhibition activities. A series of substituted dehydrozingerones [(E)-4-phenyl-3-buten-2-ones] were prepared in admirable yields by the reaction of appropriate benzaldehydes with acetone and the products were evaluated in terms of variation in the dehydrozingerone structure. The synthetic analogues were examined for their antioxidant and antityrosinase activities to probe the most potent analogue. Compound 26 inhibited Fe$^{2+}$-induced lipid peroxidation in rat brain homogenate with an IC$_{50}$ = 6.3${\pm}$0.4 ${\mu}$M. In the 1,1-diphenyl- 2-picrylhydrazyl (DPPH) radical quencher assay, compounds 2, 7, 17, 26, 28, and 29 showed radical scavenging activity equal to or higher than those of the standard antioxidants, like ${\alpha}$-tocopherol and ascorbic acid. Compound 27 displayed superior inhibition of tyrosinase activity relative to other examined analogues. Compounds 2, 17, and 26 exhibited non-competitive inhibition against oxidation of 3,4- dihydroxyphenylalanine (L-DOPA). From the present study, it was observed that both number and position of hydroxyl groups on aromatic ring and a double bond between C-3 and C-4 played a critical role in exerting the antioxidant and antityrosinase activity.

• 동의생리병리학회지
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• 제34권5호
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• pp.229-237
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• 2020

The purpose of this study was to investigate the antioxidant and hangover cure effects of compound prescription containing Phyllanthus emblica and Azadirachta Indica leaf extract (CP). In vitro experiments, HepG2 cells were induced oxidative stress by hydrogen peroxide (H2O2) and treated with CP at 50, 100, 200 ㎍/㎖ concentration. Antioxidant enzyme (superoxide dismutase (SOD), catalse (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) activity and glutathione (GSH) content were decreased by hydrogen peroxide-induced oxidative stress, but CP was increased that. In vivo experiments, experiment rats were orally administered alcohol 3 g/kg and, after 30 min administered CP 200 mg/kg. After 1 and 3 h of alcohol administration, blood was collected from the tail vein, while after 5 h, blood was collected from the heart. CP modulates alcohol dehydrogenase (ADH) and acetaldehyde level, thereby decreased alcohol level in serum. Also, CP decreased the levels of aspartate aminotransferase (AST) and alkaline phosphatase (ALP). These results suggest that CP has antioxidant effects and alleviates alcohol hangover symptoms.

• Natural Product Sciences
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• 제19권4호
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• pp.366-371
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• 2013

Seventeen compounds (1 - 17), ${\beta}$-sitosterone (1), lupenone (2), arborinone (3), ${\beta}$-sitosterol (4), lupeol (5), epi-lupeol (6), taraxerol (7), betulinic acid (8), 24R-methyllophenol (9), germanicol (10), hexatriacontane (11), nonacosan-1-ol (12), benzoic acid (13), tetradecyl(E)-ferulate (14), di(2-ethylhexyl) phthalate (15), trilinolein (16) and monopalmitin (17), were isolated from the methylene chloride-soluble fraction of the cork of Euonymus alatus Sieb. The structures of these compounds were elucidated on the basis of spectroscopic evidence. Compounds 6, 11, 13 and 14 were isolated for the first time from this plant. Compound 4 showed moderate cytotoxic activity with an $IC_{50}$ value of 6.22 ${\mu}M$ in HL-60 cell line. Compound 9 exhibited moderate cytotoxic activity with $IC_{50}$ values of 63.31, 15.45, 15.14 and 21.72 ${\mu}M$ in four kinds of human cancer cell lines, Jurkat T, HeLa, HL-60 and MCF-7, respectively. Compound 17 showed moderate cytotoxic activity with an $IC_{50}$ value of 70.71 ${\mu}M$ in Jurkat T cell line. In addition, compounds 2, 3, 14 and 16 exhibited weak antioxidant activity with $IC_{50}$ values of 151.76, 170.79, 137.46 and 139.37 ${\mu}M$, respectively.

• Advances in Traditional Medicine
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• 제8권2호
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• pp.135-145
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• 2008

The study was aimed at evaluating the antioxidant and free radical scavenging activities of ethanolic extract and its fractions of Cleome rutidosperma. The antioxidant activity, reducing power, total phenolic content, total flavonoid content, superoxide anion scavenging activity, nitric oxide anion scavenging activity, in vitro antilipid peroxidation activity and in vitro non-enzymatic hemoglobin glycosylation were studied. The results obtained in the study indicate that Cleome rutidosperma is a potential source of natural antioxidant. All the parameters were found to be concentration dependent and increased with increasing amounts of sample. Flavonoids, phenolic compound like tannins, terpenoids may be responsible for the antioxidant activity of the plant. Variation of solubility parameters in various models may be attributed to non-linearity of activity of ethanol extract fractions models. Further investigation on the isolation and identification of antioxidant component(s) in the plant may lead to chemical entities with potential for clinical use.

• 생약학회지
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• 제33권3호통권130호
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• pp.173-176
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• 2002

In search for the plant-derived antioxidant compound, it was found that the EtOAc, BuOH extracts obtained from the leaves parts of Oryza sativa L. which exhibited a significant antioxidant activity from 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical cleavage. Activity-guided fractionation on the basis of the inhibitory activity upon the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical cleavage. and repeated column chromatography afforded several antioxidant compounds from Oryza sativa L. The structures and stereochemistry of these compounds were established on the basis of analysis of spectra and some chemical transformations as follows: 5,7-dihydroxy- 8-methoxyflavone, $acacetin-7-O-{\beta}-rutinoside$, $pectolinarigenin-7-O-{\beta}-rutinoside$. At antioxidant activity test for isolated three compounds, antioxidant activity was showed too.

• 생약학회지
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• 제27권3호
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• pp.190-195
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• 1996

Wildbean(Glycine soja Siebold et Zucc.) is known as the orgin of soybean (Glycine max Merr.). Based on the hypothesis that the secondary transformation of chemical compound in wildbean might have occurred during its breed improvement to soybean. this study was carried out to compare the antioxidant activities and chemical composition in wildbean and soybean. The present study demonstrates that 1) Antioxidant activity was much higher in EtOAc, extract of wildbean than in soybean. 2) strong antioxidant activity observed in EtOAc extract of wildbean was due to the presence of (-)-epicatechin, which was not present in the extract of soybean but isolated, for the first time, from the extract of wild bean, and 3) antioxidant activity of the isolated (-)-epicatechin was greater than that of tocopherol, the previously known antioxidant.

• 생약학회지
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• 제44권3호
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• pp.224-229
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• 2013

Bromotopsentin (BSM) is a bisindole alkaloid compound, which is recognized as a metabolite of the marine sponge Spongosorites sp. In this study, the antioxidant activity of BSM was investigated. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay, the trolox equivalent antioxidant capacity (TEAC) assay, the superoxide radical scavenging (NBT) assay, the lipid peroxidation and hydroxyl radical-induced DNA damage assays were carried out to evaluate the antioxidant activity of BSM. It was found that BSM had stronger scavenging activity on the stable free radical DPPH and superoxide radical than L-ascorbic acid with an $IC_{50}$ value of 62 and 64 ${\mu}M$, respectively. The TEAC value which indicated the total antioxidant capacity of BSM was about 0.8, which was also stronger than L-ascorbic acid. About 1.3 ${\mu}M$ of BSM induced 50% inhibition of lipid peroxidation. 60 nM of BSM exhibited a significant protective activity against DNA strand scission by hydroxyl radical on pBR322 DNA. Taken together, we suggest that BSM possesses strong antioxidant activity, and could be a valuable new addition to the list of anti-aging chemotherapeutic agents.

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2006년도 제47회 학술심포지움 및 추계국제학술대회
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• pp.61.1-61.1
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• 2006

• Applied Biological Chemistry
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• 제44권1호
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• pp.35-37
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• 2001

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.211.1-211.1
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• 2003

On the purpose of develcpement of antioxidative compound from natural sources, medicinal plants known to have antioxidant actuvity have been examined concening DPPH radical scavenging activity and SOD-liked activities. Among 8 plants exhibiting the activity, Houttuynia cordata THUNB was selected as resources to search for active compounds due to rareness of study. The antioxidative compounds from Houttuynia cordata THUNB,quercitrin was assayed using a DPPH free radical. The DPPH radical scavenging activity of quercitrin was similar to that of BHA and Ascorbic acid