• 한방안이비인후피부과학회지
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• 제24권3호
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• pp.17-26
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• 2011

Objectives : Corydalis tuber has been used for promoting blood circulation and for relieving pain in Oriental medicine. In the present study, we focused on the antimicrobial activity of Corydalis tuber and compared its antimicrobial activity with the processed Corydalis tuber. Methods : Processing of Corydalis tuber was accomplished by immersing in 5% of acetic acid for 12 h and then by roasting at $250^{\circ}C$ for indicated time periods(0-30 min). Minimum inhibitory concentration(MIC) and the zone of growth inhibition were determined against Propionibacterium acnes(P. acnes). Results : The methanolic extracts of Corydalis tuber showed potent antimicrobial effect(MIC 62.5 ${\mu}g/ml$). Its alkaloidal component, dehydrocorydaline, also exhibited antibacterial activity(MIC 25.0 ${\mu}g/ml$). After processing of Corydalis tuber, its inhibitory effect on the growth of P. acnes was significantly enhanced compared with that of unprocessed Corydalis tuber. Furthermore, elevated content of dehydrocorydaline was found in the processed than the unprocessed Corydalis tuber. However, the different roasting minutes effected on antimicrobial activity. The best roasting time of Corydalis tuber was 10 min, while roasting for the time above 15 min resulted in diminishing antimicrobial activity. Thus, it was concluded that the standardized processing condition of Corydalis tuber should be established to obtain enhanced antimicrobial(P. acnes) activity. Conclusion : For antimicrobial effect against P. acnes, the best processing condition of Corydalis tuber is immersing in 5% of acetic acid for 12 h and by roasting at $250^{\circ}C$ for 10 min.

• KSBB Journal
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• 제18권5호
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• pp.393-397
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• 2003

수용성 절삭유에서 발생빈도가 높고 생존ㆍ성장력이 우수한 Pseudomonas aeruginosa를 사용하여 방청제가 방부제 (Kathon 886 MW, Triadine 3, Triadine 10 and Grotan BK)의 항균효능에 미치는 영향을 조사하였다. 실험결과 방부제와 방청제를 함께 사용할 경우 방부제의 효능은 첨가되는 방청제에 의해 그 영향을 받았다. Kathon 886 MW 항균효능은 SS 510과 MEA를 각각 첨가하였을 때 증가하였다. Triadine 3, Triadine 10, Grotan BK는 사용된 방청제에 대해 유사한 경향의 향균효능을 보였는데, CP-105, CP-E-7, MEA에 대해서 상승효과를 보였다. 그리고 방청제 고유의 항균력을 비교해 본 결과, CP-E-7과 MEA는 bioresistant 하였으며, 다른 방청제 (CP-105, AMIDE, SS-510, TEA)는 biosupportive하였다. 방부제 고유의 항균력을 비교해본 결과, Triadine 10 ＜ Triadine 3 < Kathon 886 MW < Grotan BK 순으로 항균성이 좋게 나타났다.

• Journal of Nutrition and Health
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• 제37권8호
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• pp.655-661
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• 2004

This study was performed to investigate the antimicrobial effect of the Pulsatilla koreana extracts against food-borne pathogens. First, the Pulsatilla koreana was extracted with methanol at room temperatures, and fractionation of the methanol extracts from Pulsatilla koreana was carried out by using petroleum ether, chloroform, and ethyl acetate, and methanol respectively. The antimicrobial activity of the Pulsatilla koreana extracts was determined using a paper disc method against food-borne pathogens and food spoilage bacteria. The ethyl acetate extracts of Pulsatilla koreana showed the highest antimicrobial activity against Staphylococcus aureus, Salmonella enteritidis and Shigella dysenteriae. The Staphylococcus aureus and Shigella dysenteriae were inhibited by petroleum ether and chloroform extracts of Pulsatilla koreana as well as ethyl acetate extracts of Pulsatilla koreana. The synergistic effect has been found in combined extracts of Pulsatilla koreana and Portulaca oleracea as compared to each extracts alone. Finally, the growth inhibition curve was determined using ethyl acetate extracts of Pulsatilla koreana against Staphylococcus aureus and Shigella dysenteriae. The ethyl acetate extract of Pulsatilla koreana showed strong antimicrobial activity against Staphylococcus aureus at the concentration of 2,000 ppm. The 2,000 ppm of ethyl acetate extract from Pulsatilla koreana retarded the growth of S. aureus more than 12 hours and Shigella dysenteriae up to 9 hours.

• 동아시아식생활학회지
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• 제15권2호
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• pp.182-187
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• 2005

This study was performed to investigate the antimicrobial effect of the Viscum album var. coloratum extracts against food-borne pathogens. First, the Viscum album var. coloratum was extracted with methanol at room temperature and the fractionation of the methanol extracts was carried out by using petroleum ether, chloroform, and ethyl acetate, and methanol, respectively. The antimicrobial activity of the Viscum album var. coloratum extracts was determined by using a paper disc method against food-borne pathogens and food spoilage bacteria. The petroleum ether extracts of Viscum album var. coloratum showed the highest antimicrobial activity against Bacillus cereus and Shigella dysenteriae. Synergistic effect in inhibition was observed when Viscum album var. coloratum extract was mixed with Perillae folium extract as compared with each extract alone. Finally, the growth inhibition curves were determined by using petroleum ether extracts of Viscum album var. coloratum against Bacillus cereus and Shigella dysenteriae. The petroleum ether extract of Viscum album var. coloratum had strong antimicrobial activity against Bacillus cereus at the concentration of 5,000 ppm. At this concentration, the growth of Bacillus cereus was retarded more than 24 hours and up to 12 hours for Shigella dysenteriae. In conclusion, the petroleum ether extracts of Viscum album var. coloratum inhibit efficiently Bacillus cereus and Shigella dysenteriae.

• 한국재료학회지
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• 제29권12호
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• pp.735-740
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• 2019

The purpose of this study is to fabricate an ophthalmic lens by copolymerizing two types of carbon nanotubes and hydrophilic hydrogel lens materials, and to investigate its application as an ophthalmic lens material by analyzing its physical properties and antimicrobial effect. For polymerization, HEMA (2-hydroxyethyl methacrylate), EGDMA (ethylene glycol dimethacrylate), a crosslinking agent, and AIBN (azobisisobutyronitrile), an initiator, are used as a basic combination, and a single-walled carbon nanotube and a single-walled, carboxylic-acid-functionalized carbon nanotube are used as additives. To analyze the physical properties, the water content, refractive index, breaking strength, and antimicrobial effect of the fabricated lenses are measured. The fabricated lenses satisfies all the basic properties of the basic hydrogel ophthalmic lens. The water content increases with increasing amount of additive and decreases with addition of 0.2 % ratio of nanoparticles. The refractive index is inversely proportional to the water content result. As a result of the antimicrobial test of the fabricated lens, the addition of carbon nanotubes shows an excellent antimicrobial effect. Therefore, it is considered that the fabricated lens can be applied as a functional material for basic ophthalmic hydrogel lenses.

• Journal of Microbiology and Biotechnology
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• 제21권11호
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• pp.1199-1202
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• 2011

Infection of surgical wounds is a severe problem. Conventional tissue reattachment methods have limits of incomplete sealing and high susceptibility to infection. Medical adhesives have several advantages over traditional tissue reattachment techniques, but still have drawbacks, such as the probability of infection, low adhesive strength, and high cytotoxicity. Recently, a new medical adhesive (new-adhesive) with high adhesive strength and low cytotoxicity, composed of aldehyded dextran and ${\varepsilon}$-poly(L-lysine), was developed. The antimicrobial activity of the new-adhesive was assayed using agar media and porcine skin. In the agar diffusion method, inoculated microorganisms that contacted the new-adhesive were inactivated, but this was not dependent on the amount of new-adhesive. Similar to the agar media results, the topical antimicrobial effect of new-adhesive was confirmed using a porcine skin antimicrobial assay, and the effect was not due to physical blocking based on comparison with the group whose wounds were wrapped.

• 생약학회지
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• 제49권2호
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• pp.155-164
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• 2018

Nypa fruticans Wurmb is a species of palm, which is widely distributed in the mangrove forest of Southeast Asia. Various parts of N. fruticans has been used as a traditional medicinal plant. However, the physiological activities of N. fruticans has not yet been clarified well. Therefore, in this study, the 50% ethanol extract and its aqueous and ethyl acetate fractions of young shoots of N. fruticans were investigated for their antioxidant, cytoprotective effect, and antimicrobial activities. Every sample possessed very high free radical and various ROS scavenging capacities assessed by employing different in vitro assays such as $DPPH^{\cdot}$, $O_2^{{\cdot}-}$, ${\cdot}OH$, and $^1O_2$ scavenging activities. Based on these results, the cytoprotective effect was investigated using the oxidative hemolysis of erythrocyte. We found that the extract and fractions provide a greater protective effect compared with (+)-${\alpha}$-tocopherol. Furthermore, antimicrobial activities were confirmed against skin pathogens by broth microdilution assay. The ethyl acetate fraction had much higher antimicrobial activities than methyl paraben against Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, and Candida albicans. Taken together, our results indicated that the young shoots of N. fruticans may have the potential role as a natural active ingredient through their antioxidant, cytoprotective effect, and antimicrobial activities.

• Korean Chemical Engineering Research
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• 제56권2호
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• pp.240-244
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• 2018

There is growing concern about cross infection among the patients to patients, patients to staffs, and tools to patients in healthcare facilities, especially in dentistry. In this study, the most widely used dental impression materials were prepared and the synergy effect of Chlorhexidine and essential oil on antimicrobial activity was examined in the impression materials. Chlorhexidine concentration of 0.1 wt% and 0.5 wt% showed no antimicrobial activity on Escherichia coli (E. coli) and Candida albicans. At 1.0 wt% Chlorhexidine, 0% of E. coli and 34.7% of Candida albicans were survived. Bergamot (Essential oil) concentration of 0.5 wt% and 1.0 wt% showed no antimicrobial activity on E. coli. At 2.0 wt% Bergamot oil, 71.9% of E. coli were survived. Tea tree oil (Essential oil) of 0.5 wt% showed no antimicrobial activity on E. coli. At 1.0 wt% Tea tree oil, 11.2% of E. coli was survived. At 2.0 wt% Tea tree oil, no E. coli was survived. However, no E. coli was survived at the concentration of 0.8 wt% Bergamot with 0.3 wt% Chlorhexidine. At the concentration of 0.8 wt% Tea Tree oil with 0.3 wt% Chlorhexidine, 1.3% of E. coli were survived. The experimental results showed that the synergy effects between Chlorhexidine and essential oils on antimicrobial activity were prominent.

• 한국식품조리과학회지
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• 제14권1호
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• pp.114-118
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• 1998

In ordo. to develop the natural food preservative agent, freeze dried dandelion (Taraxcum platycarpum) was extracted with several solvents, and antimicrobial activity was investigated. The methanol extract obtained from the dandelion exhibited antimicrobial properties against five strains such as Bacillus subtilis, Escherichia coli, Staphylococcus aureus, Listeria monocytogenes, and Vibrio parahaemolyticus. The methanol extract at the concentration of 2000 $\mu\textrm{g}$/ml completely inhibited the growth of B. subtilis, E. coli, L. monocytogenes and V. parahaemolyticus. Antimicrobial activity of the ethylacetate fraction from the methanol extract of dandelion was the strongest fraction compare to those the other solvent fractions such as n-hexane, chloroform, n-butanol and water. The ethylacetate fraction showed the inhibitory effect at the concentration of 0.5 mg/disc on the growth of the food spoilage microorganisms.

• Journal of Microbiology and Biotechnology
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• 제11권2호
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• pp.281-286
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• 2001

Since the number of amino groups which are exposed by deacetylation of acetyl-D-glucosamine influences antimicrobial activity, a chitosan oligosaccharide (COS) derivative by N-conjugation of COS with asparagine, an amino acid with two amino groups, was synthesized and the antimicrobial effect on E. coli growth was compared with other COS derivatives which were N-conjugated with glycine, alanine, aspartic acid, cysteins, an methionine, and unmodified COS. The structure of asparagine N-conjugated COS (Asn-COS) derivative was identified by using a FT-IR, $^{13}C\;FT-NMR$, and an elemental analyzer. The antimicrobial activity of Asn-COS against E. coli growth was significantly improved as compared to the other COS derivatives as well as COS itself. This means that Asn-COS with two positive charges strongly interacts with the carboxyl negative charges on the bacteria cell wall. The results for Asn-COS were as follows: 100% bactericidal activity, 0.002% MIC, and no growth of E. coli during 3 days of culture time, suggesting that Asn-COS may be useful as a new antibiotic agent.