• Journal of Microbiology and Biotechnology
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• 제14권1호
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• pp.15-21
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• 2004

The immunomodulating activities and chemical characteristics of a water-soluble exopolymer from submerged mycelial culture of Phellinus pini were studied. Anticomplementary activity of this polymer was found to be $73.2\%$, and its activation system occurred through both classical and alternative pathways, where the classical pathway was detected to be the major one by crossed immunoelectrophoresis. Nitric oxide (NO) release ability and acid phosphatase activity of macrophage were increased by 1.6-fold ($100{\mu}g/ml$) and 3.4-fold ($500{\mu}g/ml$), respectively, and splenocyte proliferation in mixed lymphocyte reaction (MLR) was also increased by 2.6-fold ($200{\mu}g/ml$), compared to the control. The molecular weight of this polymer, determined by HPLC, was under 5 kDa. Total sugar and protein contents were 89.7 and 10.3%, respectively. Both sugar and amino acid compositions of the exopolymer were also analyzed.

• Applied Biological Chemistry
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• 제40권2호
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• pp.152-156
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• 1997

도라지는 약리성분을 많이 함유하고 있으며 식품 및 생약재료로 널리 사용되고 있다. 재배도라지 24년근 및 3년근의 주요성분을 동정하기 위하여 약리성분을 분석하고 비교하였다. 재배도라지의 에탄올 추출물에서는 24년근에서 역상column을 사용한 HPLC분석에서 retention time이 약 50분 이후부터 3년근에는 보이지 않았던 peak가 나타나고 있어 연수에 따라 다수의 약리성 물질이 생성되거나 미량물질이 생성되었다. 재배도라지의 sterol 조성비 및 saponin 함량에서는 24년근과 3년근간에는 별다른 차이가 없었다. 재배도라지 15년근및23년근, 인삼6년근, 더덕3년근 모두 유의성있는 항보체 활성효과는 없었다.