• 공업화학
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• 제24권4호
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• pp.416-422
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• 2013

알지네이트에 은이온을 첨가하기 위하여, 질산은 수용액을 이용하여 은-알지네이트를 제조하였다. 본 연구에서는 은-알지네이트를 Poly vinylpyrrolidone (PVP) 수용액과 블렌드하였고, 전기방사는 블렌드 용액을 이용하여 수행하였다. 은-알지네이트/PVP 혼합 용액의 항균효과는 colony counting test로 대장균과 포도상 구균에 대해 확인하였다. 은-알지네이트/PVP 혼합용액의 전기방사 조건은 조성물의 농도를 다양하게 하여 방사거리 22 cm, 방사속도 0.01 mL/min, 전압 26 kV 조건하에서 수행하여 나노섬유를 제조하였다. 은-알지네이트 나노 섬유의 형태와 크기는 SEM과 Image J를 통해 확인하였으며, 전기방사된 SA5P15 섬유들의 평균 직경은 124 nm를 보였으며, 균일하게 방사되는 것을 확인하였다. SA5P15의 균 감소율은 24 시간 후 99.9%를 보였다.

• Advances in Traditional Medicine
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• 제3권1호
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• pp.40-45
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• 2003

We studied the effect of Bamboo salt-pro on the growth and acid production of S. mutans. The growth of S.mutans was reduced by the presence of the Bamboo salt-pro (1 mg/ml) and NaCl (1 mg/ml) significantly, and the positive control group (1 % of NaF) also exhibited antibacterial activity significantly. Bamboo salt-pro (1 mg/ml) reduced the rate of acid production by S. mutans. Bamboo salt alone did not demonstrate such a reduction in acid production at the concentration of 1 mg/ml. The inhibitory action of Bamboo salt-pro on acid production was found at a concentration of 1 mg/ml, but bamboo salt alone was not at a concentration of 1 mg/ml. In addition, we investigated the anti-inflammatory effect of Bamboo salt-pro on human mast cell line HMC-1. Bamboo salt-pro (0.1 and 0.01 mg/ml) inhibited significantly the secretion of inflammatory cytokine, tumor necrosis factor-a with $59.47{\pm}0.15%$, $51.98{\pm}0.16%$ respectively. Our results suggest that Bamboo salt-pro importantly contributes to the prevention or treatment of periodontitis and other oral diseases and inflammatory diseases.

• 한국산업보건학회지
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• 제31권2호
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• pp.138-146
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• 2021

Objectives: This study aims to compare and evaluate the reduction efficiency of disinfectants used in the domestic meat processing industry to reduce bioaerosol exposure of meat industry workers and to use this as basic data for establishing work environment management measures. Methods: Thirteen disinfectants sold in South Korea were selected for evaluation and the bacterial reduction effect of the disinfectants was investigated. Bacterial suspension and surface disinfection tests were conducted to compare and analyze the antibacterial strength of the disinfectants. Pork carcasses, cutting boards, benches, and conveyor belts were selected for surface sterilization tests. Results: As a result of the bacterial suspension experiment test, all disinfectants had a bacterial reduction efficiency of more than 86%. Among them, the bacterial reduction efficiency of chlorine disinfectants was 99.93% on average. In the results of the pork carcass surface sterilization test, the rate of reduction of disinfectants made of quaternary ammonium compounds (QACs) was the highest. Tests of plastic cutting boards showed that chlorine disinfectants had the best sterilization effect. Experiments on stainless steel benches showed the best bacterial reduction efficiency for chlorine dioxide and QACs disinfectants. In the conveyor belt made of urethane, QACs disinfectants showed excellent sterilization effects. Conclusions: The study evaluated the disinfection power of disinfectants against bacteria occurring in domestic meat processing plants. All disinfectants were found to be effective in bacterial suspension experiments, and chlorine disinfectants were particularly effective. In surface sterilization experiments, sterilizing agents with QACs as the main ingredient were excellent.

• 한국지역사회생활과학회지
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• 제28권1호
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• pp.143-154
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• 2017

This study was conducted to develop fabrics using non-toxic and eco-friendly natural dyes made from curcuma and coffee extract. The natural dyes were applied to non-printed cotton fabrics using the dip dyeing method, and the pattern was hand-printed onto the dyed fabrics to make dyed printed-fabrics. The four types of developed fabric samples were compared to analyze the effects of the dyeing materials and printing on the hygienic properties of curcuma- and coffee-dyed fabrics. The findings were as follows. First, air permeability of both curcuma- and coffee-dyed fabrics was greatly affected by the presence of patterns, although the type of dye did not seem to have an effect. Printing greatly reduced air permeability of the fabric, and coffee-dyed fabric showed greater reduction of dyeing method than curcuma-dyed fabric. Moisture regain also tended to decrease with printing, but the change was much smaller compared to air permeability. Second, increased UV blockage was observed in curcuma- and coffee-dyed fabrics with patterns compared to non-printed fabrics, showing that printing enhanced UV blocking. Third, deodorization rate tended to increase sharply for both curcuma- and coffee-dyed fabrics until 30 minutes and gradually increased thereafter to yield 30% for curcuma and 50% for coffee at 120 minutes, demonstrating higher deodorization of coffee dye. Coffee-dyed fabric showed an antibacterial rate about twice as high as that of curcuma-dyed fabric, and the observed data suggest that curcuma-dyed fabric had an insignificant level of antibiosis. Fourth, printing significantly enhanced wash, sunlight, and compound colorfastness of the two types of dyed fabrics. The effect of printing was most dramatic on sunlight and compound colorfastness, which are aspects in which natural dyed fabrics perform poorly in general. Eventually, the development and application of biologically- and environmentally-friendly fabrics with natural dyes correspond with increased interest towards the wellness and healthy attitudes of modern society.

• 한국염색가공학회지
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• 제25권4호
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• pp.314-326
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• 2013

The purpose of this research is to analyze the effects of Dansam and Sappan wood extract to perform combination dyeing on silk fabrics, with respect to color changes, fastness (washing, dry cleaning, perspiration, rubbing and light fastness), and functionality (antibacterial activity and deodorization). Combination dyeing was performed by first combining Dansam with Sappan wood, then Sappan wood with Dansam, in these orders. Given the changes in the combination ratio, pre-mordant treatment was performed. Looking at the surface colors of each dye, Dansam generally produces YR color series, while Sappan wood produces YR, R, and RP color series. The effects of changing the order in which combination dying was performed on the surface colors were as follows. First, combination dyeing (A) was performed by using Dansam before Sappan wood, to produce YR and R color series. Then combination dyeing (B) was performed by using Sappan wood before Dansam, to produce YR, R, and RP color series. By visual inspections, more similar color changes of the combination dyeing were noticed with the post-dyeing material rather than the pre-dyeing material. Therefore, it was presumably confirmed that surface color changes of combination dyeing were greatly influenced by the post-dyeing color. Individual dyeing tests for fastness showed that Dansam was comparatively superior to Sappan wood, which demonstrated lower fastness to washing, dry cleaning, perspiration, and light, relatively. The fastness of combination dyed samples was shown middle, but similar fastness to the post-dye material, The fastness of (B) method was higher than (A) method in the washing and light fastness. This confirms that color fastness from combination dyeing was considerably influenced by the post-dye material. It was found that all dyed samples had a very high bacterial reduction rate of 99.9% and high deodorization rate of 95%.

• Journal of Microbiology and Biotechnology
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• 제24권4호
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• pp.522-533
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• 2014

Bionanotechnology has revolutionized nanomaterial synthesis by providing a green synthetic platform using biological systems. Among such biological systems, microalgae have tremendous potential to take up metal ions and produce nanoparticles by a detoxification process. The present study explores the intracellular and extracellular biogenic syntheses of silver nanoparticles (SNPs) using the unicellular green microalga Scenedesmus sp. Biosynthesized SNPs were characterized by AAS, UV-Vis spectroscopy, TEM, XRD, FTIR, DLS, and TGA studies and finally checked for antibacterial activity. Intracellular nanoparticle biosynthesis was initiated by a high rate of $Ag^+$ ion accumulation in the microalgal biomass and subsequent formation of spherical crystalline SNPs (average size, 15-20 nm) due to the biochemical reduction of $Ag^+$ ions. The synthesized nanoparticles were intracellular, as confirmed by the UV-Vis spectra of the outside medium. Furthermore, extracellular synthesis using boiled extract showed the formation of well scattered, highly stable, spherical SNPs with an average size of 5-10 nm. The size and morphology of the nanoparticles were confirmed by TEM. The crystalline nature of the SNPs was evident from the diffraction peaks of XRD and bright circular ring pattern of SAED. FTIR and UV-Vis spectra showed that biomolecules, proteins and peptides, are mainly responsible for the formation and stabilization of SNPs. Furthermore, the synthesized nanoparticles exhibited high antimicrobial activity against pathogenic gram-negative and gram-positive bacteria. Use of such a microalgal system provides a simple, cost-effective alternative template for the biosynthesis of nanomaterials in a large-scale system that could be of great use in biomedical applications.

• 미생물학회지
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• 제51권4호
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• pp.407-418
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• 2015

본 연구는 백김치로부터 분리된 Lactobacillus paracasei BK57의 항균물질 생산을 위한 최적의 배양 조건을 검색하고, BK57 유산균으로 프레쉬 치즈를 제조한 후 균주의 활성과 유산 및 박테리오신 생산량을 측정하여 Listeria monocytogenes KCTC 3569에 대한 항균 활성을 조사하였다. 최대의 균 증식과 항균 물질 생산량은 pH 6.0으로 조정한 MRS broth에서 $37^{\circ}C$, 24시간 동안 호기적인 조건으로 배양했을 때 나타났다. 하지만, 효모추출물(2.0%)을 첨가한 전유 내에서 생성된 항균물질의 양과 유산균의 증식률은 MRS broth에서 보다는 다소 낮았다. 우유 내에서 L. monocytogenes의 저해율은 BK57 균주의 생균과 배양 상등액에 의해 높게 나타났으나, 유산균이 생산한 박테리오신에 의한 저해율은 우유 보다는 BHI broth 내에서 더 높게 나타났다. BK57 균주로 발효시킨 프레쉬 치즈를 $4^{\circ}C$와 $15^{\circ}C$에서 6일간 저장하는 동안 유산균수, 유산 생성량 및 박테리오신 활성은 유의한 변화가 없었다. 제조 직 후 프레쉬 치즈에 인위적으로 접종한 L. monocytogenes ($10^5CFU/ml$)의 균수는 각각의 온도대에서 6일 이내에 최소 15% 이상 감소되는 효과가 나타났으므로 BK57 균주를 발효유제품 제조에 이용할 경우 리스테리아균을 제어할 수 있는 생물학적 보존제로서의 가치를 확인하였다.

• 대한의생명과학회지
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• 제16권1호
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• pp.68-70
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• 2010

Puer tea is a traditional beverage originating from Yunnam area of China. It supplies basic nutrients such as vitamin C. It has been well reported that daily drinking of Puer tea can help the digestion and ease the stomachache after food intake. Puer tea also contains various polyphenols which may exert antibacterial activity against Staphylococcus aureus and Listeria monocytogenes. Because of these functional effects on digestive system we suspected if Puer tea can display any dietary effect or decrease the obesity after long-term drinking. We employed 6-week old SD rats as experimental animal and treated them with extract of Puer tea in relation to the body weights. Rats were divided into 5 groups (NC, PC, E, E+P, E+P5). NC group was experimental control and rest of them are as follows: water only (PC), water with exercise (E), water with exercise and Puer tea extract (E+P), water with exercise and 5X extract of Puer tea (E+P5). Feeding was carried out every day for 5 weeks by oral administration. Reduction rate of body weights was highest in E group. Relative ratio of losing weight was as follows: PC group (100.78%), E group (95.57%), E+P group (94.53%) and E+P5 group (74.22%), respectively. Exercise was more helpful to control the body weight. The result strongly suggests that Puer tea is highly effective to control the body weight and could be used for pharmaceutical purpose to treat obesity without side effects.

• 한국임상수의학회지
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• 제27권1호
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• pp.17-22
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• 2010

천연물 유래 항생제 대체 사료첨가제 Flavo-$SK^{TM}$은 원광대학교 인수공통감염병연구센터에서 1,000종 이상의 천연물로부터 효능평가를 거쳐 개발되었다. 본 연구는 육계 농장에서 대규모 임상 효능평가를 통한 Flavo-$SK^{TM}$의 항균 효과 규명과 항생제 대체 효과를 알아보고자 수행되었다. 20,000 마리의 병아리를 Flavo-$SK^{TM}$ 투여군과 비투여군 2 그룹으로 나누어 31일 동안 육계농장에서 연구가 수행되었다. Flavo-$SK^{TM}$ 투여군에는 항생제가 없는 사료에 Flavo-$SK^{TM}$를 0.29% 배합하여 급여하였고 비투여군은 항생제 포함된 일반 사료를 급여하였다. 연구기간 동안 사료섭취량, 체중, 임상증상을 분석하고 시험종료일에 부검하여 육안검사와 미생물학적 검사 및 병리조직학적 검사를 수행하였다. 연구결과 천연물 유래 항생제 대체 사료첨가제 Flavo-$SK^{TM}$은 육계에 항균효과를 가진 항생제 대체 사료첨가제로서 효과적인 것을 확인할 수 있었다.

• 생명과학회지
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• 제17권11호
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• pp.1555-1561
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• 2007

본 연구는 무기물성 및 식물성 유래 생리활성물질로서 scoria, germanium, charcoal, 생강, stevia 및 CLA(conjugated linoleic acid)가 병원성 미생물 및 반추위 미생물에 대한 작용을 조사하기 위하여 수행되었다. 병원성 균으로서 Escherichia coli O157, Salmonella paratyphi, Listeria monocytogenes 및 Staphylococcus aureus에 적용하여 항균활성을 측정하고, in vitro 발효 시 반추위 미생물 성장률, 가스 생성량, 암모니아 농도, CMCase 활성 및 미생물의 수를 측정하였다. 병원성 미생물 배양액에 생강을 0.1% 첨가한 구에서만 항균활성이 나타났으나, paper disc법에 의한 항균활성 시험에서는 stevia 10%첨가구와 CLA 10% 첨가구에서 E. coli에 대해서 항생제 첨가구인 positive control 구와 비슷한 크기의 clear zone을 형성하였다. in vitro 반추위 미생물 발효시험에서는 생강, stevia 및 CLA가 반추위 박테리아와 프로토조아의 증식을 억제하는 것으로 나타났는데, 특히 생강 첨가구의 경우 메탄 생성균의 서식지로 알려져 있는 프로토조아를 크게 억제함으로써 메탄생성 억제제로서 개발 가능성이 있는 것으로 사료된다.