• Title/Summary/Keyword: antibacterial material

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A Study on Antioxidant and Antibacterial Efficacy Evaluation and Cosmetic Application of Rumex crispus L. Root Extract (소루쟁이 뿌리 추출물(Rumex crispus L.)의 라디칼 소거능과 항균력 평가 및 화장품 적용 가능성에 관한 연구)

  • Yu-Jin Park;Jae-Chan Yang
    • Journal of the Korean Applied Science and Technology
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    • v.41 no.2
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    • pp.159-171
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    • 2024
  • This study confirmed the antioxidant activity and antimicrobial efficacy and formulation stability for the effectiveness experiment of Rumex crispus. L root extract. For antioxidant activity, DPPH radical scavenging, FRAP activity, ABTS+ radical scavenging, and SOD-like activity were performed. Antimicrobial activity was evaluated for Staphylococcus epidermidis, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Candida albicans strains. In addition, skin containing Rumex crispus. L root extract is checked over time for pH, temperature, and daylight for 21 days. As a result of antioxidant evaluation, it was confirmed that the activity increased in a concentration-dependent manner at a concentration of 0.0625-1 mg/mL. The clear zones of each bacterium at 100mg/mL concentrations were 10.45±0.34, 9.77±0.59, 9.92±0.22, and 10.08±0.12, which were superior to the control group Methyl paraben, and the antibacterial power of S. aureus and E. coli was confirmed at 100mg/mL concentration for MIC. There was little change in absorbance when the pH of the skin was 4.0, 6.0, and 7.0 and At 4℃, 25℃, and 40℃, it was discolored as the temperature increased. It was also observed that discoloration occurred when exposed to daylight. This is presumed to be able to prevent discoloration when it is shielded and stored at low temperatures. When the results of this study are summarized, Rumex crispus. L root extract is considered to have high value in use as a cosmetic raw material that can expect antioxidant and antibacterial activities.

Antioxidant and Antibacterial Activities of Grape Pomace Fermented by Various Microorganisms (발효 미생물에 따른 포도가공 부산물의 항산화 활성 및 항균활성)

  • Kim, Kyoung-Hee;Yun, Young-Sik;Chun, Se-Young;Yook, Hong-Sun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.41 no.8
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    • pp.1049-1056
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    • 2012
  • The antioxidant activities and antibacterial activities of grape pomace fermented using a variety of useful microorganisms were analyzed. There were several experimental groups: the control, with non-fermented grape pomace; the BS group, fermented by Bacillus subtilis; the LP group, fermented by Lactobacillus plantarum; the LC group, fermented by L. casei; the CU group, fermented by Candida utilis; the Y1 group, fermented by Saccharomyces cerevisiae strain CHY1011; the Y2 group, fermented by S. cerevisiae strain ZP 541; and the M group, fermented by a mixed strain culture of LP, LC, and CU. The yield of freeze-dried powder of fermented grape pomace by BS, LP, LC, CU, Y1, Y2, and M was 10.74%, 9.36%, 8.68%, 9.55%, 7.49%, 9.60%, and 9.71% w/w, respectively. The total polyphenol content of grape pomace showed the highest value in the control, but the fermented LP had higher total polyphenol content than those of other fermented grape pomace. The control and fermented LP had 0.16 mg/mL and 0.28 mg/mL as $IC_{50}$ values on DPPH radical scavenging, and 0.22 mg/mL and 0.53 mg/mL of ABTS radical scavenging activity, respectively. The FRAP value (5 mg/mL) showed the highest value on fermented LP (2.44 mM) but did not show a significant difference in the control group (12.27 mM). The fermented LC showed the antimicrobial activities against B. cereus (11 mm), B. subtilis (11 mm), Staphylococcus aureus (12 mm), Escherichia coli (12 mm), Enterobacter cloacae (10.5 mm), Salmonella enterica (11.5 mm), and Pseudomonas aerugionsa (11 mm) at 5 mg/disc, but the control and other fermented grape pomace did not show antimicrobial activities. Thus, fermented grape pomace by LC is shown to be producing a material that has antibacterial activity. In conclusion, grape pomace fermentation using various lactic acid bacteria strains showed excellent effects in promoting the production of functional materials. Especially, using L. casei exhibited an increase in antibacterial activity, and using L. plantarum exhibited antioxidant activity.

Antimicrobial Synergistic Effects of Gallnut Extract and Natural Product Mixture against Human Skin Pathogens (피부 병원성균에 대한 오배자 천연 복합물의 시너지 항균 효과)

  • Kim, Ju Hee;Choi, Yun Sun;Kim, Wang Bae;Park, Jin Oh;Im, Dong Joong
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.47 no.2
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    • pp.155-161
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    • 2021
  • This study was attempted to investigate natural materials with antimicrobial activity and to apply as natural preservatives in cosmetics. The disc diffusion method was used to search for nine species of natural antibacterial material for three species of skin pathogenic bacteria (Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa) and Candida albicans. As a result of measuring the size of inhibition zone, Rhus Semialata gall (Gallnut) extract, Oak vinegar, and ε-polylysine were showed strongest antibacterial activities (> 10 mm). The Minimum Bactericidal Concentration (MBC) of gallnut and oak vinegar ranged from 10 to 20 mg/mL and from 20 to 40 mg/mL against five human skin pathogens. The MBC of ε-polylysine ranged from 0.5 to 2 mg/mL in fungus. The synergic effect of gallnut extract/oak vinegar mixture and gallnut extract/ε-polylysine mixture were evaluated by checkerboard test. Compared to when used alone, the MBC of gallnut extract/oak vinegar mixture were at 4 times lower concentration against E. coli, C. albicans, and A. brasiliensis. Also Furthermore, the MBC of gallnut extract/ε-polylysine mixture were at 4 times lower concentration against C. albicans and A. brasiliensis. It was confirmed that the combination of gallnut extract with oak vinegar or ε-polylysine resulted in synergistic antibacterial effect against three human skin pathogens. Thus, it is expected that gallnut extract and natural product mixture can not only demonstrate antibacterial synergies, but also be applied in cosmetics as a natural preservative system with a wide antibacterial spectrum.

A Study on the Effectiveness of a Mixed Fermented Extract of Vitamin Tree Fruit, Acai Palm Fruit, Mango, Lemon, Apricot and Blueberry as an Active Ingredient (비타민나무열매, 아사이팜열매, 망고, 레몬, 살구 및 블루베리의 혼합 발효추출물의 효능 연구)

  • Kim, Su Chang;Lee, Mi Rae;Cho, Won Pyo;Yoon, Year Pill
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.48 no.3
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    • pp.235-244
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    • 2022
  • In this study, the cell viability, radical scavenging ability, elastase activity, moisturizing activity, and antimicrobial activity of mixed fermented extract of vitamin tree fruit, acai palm fruit, mango, lemon, apricot and blueberry as an active ingredient were measured. The experiment was performed at three concentrations(0.5 / 1.5 / 2%), since cytotoxicity was not shown at these three concentrations. Based on this, a radical scavenging experiment was conducted to compare with L-ascorbic acid, a representative raw material of an antioxidant material. The results showed that the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging ability was 95.1 ± 0.6%, 94.3 ± 0.7%, 95.3 ± 0.6%, 95.1 ± 0.7%, 95.1 ± 0.3%, 95.5 ± 0.3%, and 95.4 ± 0.4% respectively, when the concentration of the mixed fermentation extract was 2%. As the concentration increased at the three concentrations (0.5/1.5/2%) and the content of blueberry extracts increased, sample 7 showed excellent elastase activity at a concentration of 2% to 0.9 cm, such as retinol crystal, and moisturizing activity was also found to be 71.5%, 75.6%, and 81.6%, respectively. Particularly, at the 2% concentration of the mixed fermented extract, the antibacterial activity effect on Propionibacterium was very excellent in sample 7. Therefore, it is considered that it is worth developing the mixed fermented extracts of 2% concentration as a cosmetic component with antioxidant, moisturizing, and antibacterial properties.

Effects of a Dye from Artemisia, the Natural Dyeing Material, on the Functionality of Dyeing in Mind-Peace Education for the Silver Generation (실버세대 감성교육을 위한 천연염재 쑥속(Artemisia)식물의 염료가 염색의 기능성에 미치는 영향)

  • Lee, Jung-Eun;Kwon, Young-Suk
    • Journal of Environmental Science International
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    • v.24 no.5
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    • pp.633-639
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    • 2015
  • The experience of natural dyeing is expected to be effective in promoting health through good exercise, in giving emotional stability based on the functional aromatic components of the dyeing material, and in improving self-esteem and increasing positive experiences through the behavior of creation. A natural dyeing material easily found around us, artemisia has long been used in food and Oriental medicine and was recently found to contain antioxidative, anticancer, deodorizing, antibacterial, anti-obesity, and anti-diabetes substances through chemical analysis. It has also been reported that group reminiscence therapy using the fragrance of artemisia is effective in relieving pain and depression and in promoting ego integrity in the elderly population. This study aimed to make a comparative analysis of dyeability, washing fastness, and deodorization between Artemisia princeps, Artemisia iwayomogi, and Artemisia annua, all of which are considered to be healthful and functional dyeing materials, among about thirty domestic plants in the genus of Artemisia and to provide basic data concerning natural dyeing in mind-peace education for the silver generation. The fabric dyed and post-mordanted at $80^{\circ}C$ for 40 minutes showed the greatest surface color variation; in this condition, artemisia princes (32.29) gave the greatest color difference, followed by artemisia iwayomogi (31.07) and artemisia annua (26.17). While all the types of artemisia were excellent in washing fastness, dry-cleaning fastness, and rubbing fastness at the fourth- or fifth-grade, light fastness was at the third grade for artemisia princes, at the second to third grade for artemisia annua, and at the second grade for artemisia iwayomogi; therefore, artemisia princes was found to give better fastness than the other two types of artemisia. In determining functionality of the fabric dyed with artemisia, deodorization test also found that the fabric dyed with artemisia princes had 20% higher deodorization.

Isolation and Purification of an Antimicrobial Material from the Jellyfish Nemopilema nomurai (노무라 입깃 해파리(Nemopilema nomurai)로부터 항균활성물질의 분리 및 정제)

  • Moon, Ho-Sung;Kim, Yeon-Kye;Lee, Moon-Hee;Yoon, Na-Young;Lee, Doo-Seog;Yoon, Ho-Dong;Seo, Jung-Kil;Park, Nam-Gyu
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.44 no.5
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    • pp.478-483
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    • 2011
  • An antimicrobial material was purified from the acidified whole body extract of the jellyfish Nemopilema nomurai by using C18 reversed phase and cation-exchange high performance liquid chromatography (HPLC). Whole body extract and the purified compound (JAP-1) showed potent antimicrobial activities against a wide range of microorganisms including Escherichia coli D31, Bacillus subtilis, Streptococcus iniae and Candida albicans, without significant hemolytic activity. Treatment of JAP-1 with trypsin completely abolished all antibacterial activity against Bacillus subtilis, suggesting that JAP-1 is likely to be a proteinaceous antibiotic. The molecular weight of JAP-1 was determined to be 680.10 Da by MALDI-TOF mass spectroscopy.

Improvement in Storabilities of Rice Bran Protein Film Containing Bacteriocin Produced by Pseudomonas putida 21025 (Pseudomonas putida 21025가 생성하는 bacteriocin을 이용한 항균성 미강 단백질 필름의 저장성 향상)

  • 김은정;김경미;배동호
    • Food Science and Preservation
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    • v.10 no.3
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    • pp.411-416
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    • 2003
  • An effort was attempted to utilize an under-utilized protein source, rice bran protein, in coating or wrapping food material for the purpose of protection them from oxidation and bacterial infection. However, the utilization of rice bran protein as a food coating material is limited because the rice bran protein coating material itself can be spoiled by a bacterial infection. Therefore, this study was conducted to produce the economical and antibacterial rice bran protein film by utilizing rice bran and bacteriocin-producing microorganism. Bacteriocin produced by Pseudomonas putida 21025 was partially purified after 33h of shaking incubation at 30$^{\circ}C$. The amount of amino-type nitrogen did not increase in the rice bran protein film containing the bacteriocin any more after gradual increase upto the content of 0.22% for 8 days, while that without the bacteriocin increased continuously, implying that application of the bacteriocin to the rice bran had positive effects on prolonging the shelf-life of not only film itself but also the foods wrapped by this film.

Enhancing the Physicochemical Properties of Sodium Iodide-based Root Canal Filling Material with Lanolin Incorporation

  • Hye Shin Park;Jongsoo Kim;Joonhaeng Lee;Jisun Shin;Mi Ran Han;Jongbin Kim;Yujin Kim;Junghwan Lee
    • Journal of the korean academy of Pediatric Dentistry
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    • v.51 no.2
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    • pp.140-148
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    • 2024
  • This study aimed to enhance the physicochemical properties of sodium iodide-based root filling materials, particularly solubility. In earlier developmental stages, the iodoform-containing paste exhibited high antibacterial efficacy but failed to meet only the solubility requirement among the ISO 6876 criteria. Therefore, this study focused on enhancing the physicochemical properties of the paste under development, particularly centering on reducing its solubility. Four experimental groups were established, including three control group. The previously developed D30 paste was named the Oil 33 group, and the control group was named the Vitapex® group. The Oil 50 group, in which the oil content was increased, and the Oil 45L group, in which lanolin was incorporated. The physical properties (solubility, pH, flowability, and film thickness) of the four pastes were evaluated according to the ISO 6876 standards. No significant differences were observed between the Oil 45L and Vitapex® groups in any of the physical property evaluations. While the Oil 33 and Oil 50 groups met the ISO 6876 standards for flowability and film thickness, the Oil 45L group met all the physical properties. However, reducing the overall oil content may be necessary to enhance the antimicrobial properties. The result of the physicochemical experiments showed that the Oil 45L group with the newly formulated composition and incorporated lanolin exhibited low solubility meeting the ISO 6876 standard of ≤ 3%. We were able to develop a paste with more stable solubility than previous iodide-based root-filling materials. Therefore, the oil content must be further adjusted to improve its antimicrobial properties. If other physical properties also meet the ISO 6876 standards and demonstrate excellent results in cytotoxicity tests, this root filling material could potentially replace existing options.

Development of Green Rehabilitation Material for Rock and Abandoned Mine Debris Slopes (폐광산 암반 및 폐석사면에 적합한 친환경 식생복원 소재 개발에 대한 연구)

  • Jung, Mun-Ho;Ryu, Jong-Heum;Kim, Tae-Heok;Lee, Hak-Joo;Choo, Chang-Oh;Shin, Youn-Soo;Jeong, Gyo-Cheol
    • The Journal of Engineering Geology
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    • v.22 no.4
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    • pp.439-448
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    • 2012
  • The aim of this study is to develop eco-friendly, fundamental technology for vegetative restoration of barren lands using green rehabilitation material, for the stabilization of abandoned mine debris slopes and depository slopes composed of rock fragments. It is expected that such methods would help to improve the negative view of artificial slopes that remain following mining activity, while also contributing to slope stabilization and prevention of the loss of rock fragments and the dispersion of tailings. We tested the tensile strength and tear strength of various materials, including commonly used natural coir material, natural fibers, and synthetic textile materials with enhanced durability. In conclusion, there is the possibility that natural coir can be used as an eco-friendly material in vegetation restoration, while its durability in natural conditions can be improved by controlling the degree of knot convolution and by antibacterial treatment against biodegradation.

The Contemporary Metal Urns : Art and Symbolism (현대 금속 골호작품의 조형적 특징과 상징적 의미 연구)

  • Ahn Myung-Sun
    • Journal of Science of Art and Design
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    • v.6
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    • pp.226-248
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    • 2004
  • The researcher has tried to develop the aesthetic value of the art and the function of metalwork in conjunction with our daily life. especially has been eager to develop the new possibility of metal urns through the investigation of ordinary metalwork. Funeral urns are vessels to fill the ashes of bodies. As the social issues, including problems of restricted land territory and environmental pollution are awakening, many people are much concerned about the use of urns in recent days. Although social concerns about cremation and urns are getting high, most urns in Korea have been made of ceramic and stone. The shape of old urns are pot style with lids, and their color is simple white or jade green. Moreover, old urns are expensive, unpractical, simple, and furthermore, the feature of urns has not preserved the sense of beauty. In this context, we have narrow choices to select urns, and it has been difficult for us to be familiar with the old-style urns. Therefore, the need for developing metal urns are necessary as the increase of public concerns towards cremation is widely spreading. As it is well known, metal is not only regarded as hard, antibacterial, pro-environmental material , but also regarded as good substance for urns. Metal is also suitable for making urns because of metal's characteristics of easy processing, mass production, and cheap price. The researcher has been tried to find the possibility of art of metalwork through the dissemination of metal urns to the public. The researcher has also attempted to represent the characteristics and symbolic implications of contemporary metal urns for ordinary people to adopt metal urns as their 'Rite Haven'.

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