• 한국군사과학기술학회지
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• 제13권1호
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• pp.126-132
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• 2010

A Lamb wave guided by a plate structure has dispersive characteristics because phase and group velocity change with the variation of frequency and thickness. The Lamb wave has two modes, symmetric and anti-symmetric mode, which propagates symmetrically and non-symmetrically with respect to centerline. In this paper, the derivation of Lamb wave equation with anisotropic material property is investigated. The phase velocity and group velocity dispersion curves are shown using the stiffness matrix of composite materials with the variation of angle.

• 전력전자학회논문지
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• 제23권6호
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• pp.373-380
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• 2018

This study proposes a new islanding detection method that uses the phase shift of feed-forward voltage and evaluates the performance of an existing method and the proposed method when the grid frequency changes within the allowable range under steady-state conditions. The investigated existing method, which is slip mode frequency shift (SMS), uses current phase shift to detect islanding. The SMS method supplies reactive current to the grid under this condition, but the proposed method does not generate additional reactive power because it does not depend on the current control loop. The performance in steady-state grid condition is evaluated through simulations and experiments.

• Asian Pacific Journal of Cancer Prevention
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• 제15권24호
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• pp.10659-10663
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• 2015

Background: Rapamycin is an effective anti-angiogenic drug. However, the mode of its action remains unclear. Therefore, in this study, we aimed to elucidate the antitumor mechanism of rapamycin, hypothetically via apoptotic promotion, using MCF-7 breast cancer cells. Materials and Methods: MCF-7 cells were plated at a density of $1{\times}10^5$ cells/well in 6-well plates. After 24h, cells were treated with a series of concentrations of rapamycin while only adding DMEM medium with PEG for the control regiment and grown at $37^{\circ}C$, 5% $CO_2$ and 95% air for 72h. Trypan blue was used to determine the cell viability and proliferation. Untreated and rapamycin-treated MCF-7 cells were also examined for morphological changes with an inverted-phase contrast microscope. Alteration in cell morphology was ascertained, along with a stage in the cell cycle and proliferation. In addition, cytotoxicity testing was performed using normal mouse breast mammary pads. Results: Our results clearly showed that rapamycin exhibited inhibitory activity on MCF-7 cell lines. The $IC_{50}$ value of rapamycin on the MCF-7 cells was determined as $0.4{\mu}g/ml$ (p<0.05). Direct observation by inverted microscopy demonstrated that the MCF-7 cells treated with rapamycin showed characteristic features of apoptosis including cell shrinkage, vascularization and autophagy. Cells underwent early apoptosis up to 24% after 72h. Analysis of the cell cycle showed an increase in the G0G1 phase cell population and a corresponding decrease in the S and G2M phase populations, from 81.5% to 91.3% and 17.3% to 7.9%, respectively. Conclusions: This study demonstrated that rapamycin may potentially act as an anti-cancer agent via the inhibition of growth with some morphological changes of the MCF-7 cancer cells, arrest cell cycle progression at G0/G1 phase and induction of apoptosis in late stage of apoptosis. Further studies are needed to further characterize the mode of action of rapamycin as an anti-cancer agent.

• Journal of Power Electronics
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• 제12권4호
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• pp.623-631
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• 2012

In this paper, a digital controller design procedure is presented for single-phase voltage-doubler boost rectifiers (VDBR). The model derivation of the single-phase VDBR is performed in the s-domain. After that the simplified equivalent z-domain models are derived. These z-domain models are utilized to design the input current and the output dc-link voltage controllers. For the controller design in the z-domain, the traditional K-factor method is modified by considering the nature of the digital controller. The frequency pre-warping and anti-windup techniques are adapted for the controller design. By using the proposed method, the phase margin and the control bandwidth are accurately achieved as required by controller designers in a practical frequency range. The proposed method is applied to a 2.5 kVA single-phase VDBR for Uninterruptible Power Supply (UPS) applications. From the simulation and the experimental results, the effectiveness of the proposed design method has been verified.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2003년도 춘계학술대회논문집
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• pp.833-838
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• 2003

The problem of elastic wave resonance scattering from elastic targets is studied in this paper. A new resonance formalism to extract the elastic resonance information of the target from scattered elastic waves is introduced. The proposed resonance formalism is an extension of the works developed for acoustic wave scattering problems by the author. The classical resonance scattering theory computes reasonable magnitude information of the resonances in each partial wave, but the phase behaves in somewhat irregular way, therefore, is not clearly explainable. The proposed method is developed to obtain physically meaningful magnitude and phase of the resonances. As an example problem, elastic wave scattering from an infinitely-long elastic cylinder was analyzed by the proposed method and compared to the results by RST. In case of no mode conversion, both methods generate identical magnitude. However, the new method computes exact $\pi$ radian phase shills through resonances and anti-resonances while RST produces physically unexplainable phases. In case of mode conversion, in addition to the phase even magnitudes are different. The phase shifts through resonances and antiresonances obtained by the proposed method are not exactly $\pi$ radians due to energy leak by mode conversion. But, the phases by the proposed method show reasonable and intuitively correct behavior compared to those by RST.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제35권3호
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• pp.143-154
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• 2013

Purpose: This study was to find efficacy of integrin alpha2 (${\alpha}_2$) and epidermal growth factor receptor (EGFR) as tumor marker of oral squamous cell carcinoma (SCC) and clarify the selective cell death effect of anti-integrin ${\alpha}_2$ and anti-EGFR on SCC cells, additionally testify conjugated gold nanoparticles (GNP) with air plasma for selective cell death of oral SCC. Methods: Expression of integrin ${\alpha}_2$, EGFR on human SCC cells (SCC25) were examined by western blot. SCC25 cells were treated with anti-integrin ${\alpha}_2$, anti-EGFR and analysed by Hemacolor staining, immunoflorescence staining, FACS flow cytometry. Conjugated GNP with integrin ${\alpha}_2$, EGFR antibody were treated by air plasma on SCC cells. Results: Integrin ${\alpha}_2$ and EGFR were over-expressed on SCC25 cells than normal lung WI-38 cells. The cell viability rate of SCC25 cells treated with anti-integrin ${\alpha}_2$, anti-EGFR was lower than WI-38 cells. The concentration changes of nucleus, releasing cytochrome c and apoptosis inducing factor (AIF) from mitochondria to cytosol were observed. The changes of proteins related with apoptosis were observed. Increase of bax, bcl-xL, activation of caspase-3, -7, -9, and fragmentation of PARP, DFF45 and decrease of lamin A/C in SCC25 cells were observed. In FACS, increase of sub-$G_1$ and S phase was observed. Cell cycle related proteins, Such as cyclin D1, cyclin dependent kinase (CDK) 4, cyclin A, cyclin E, CDK 2, p27 were decreased. After SCC25 cells treated with conjugatged GNP-Integrin ${\alpha}_2$, GNP-EGFR, additionally air plasma, the cell death rate was significantly increased. Conclusion: Integrin ${\alpha}_2$, EGFR were over-expressed in oral SCC cells. Anti-integrin ${\alpha}_2$, anti-EGFR in SCC25 cells induced apoptosis selectively. When GNP-anti integrin ${\alpha}_2$, GNP-anti EGFR were treated with air plasma on SCC25 cells, cancer cells were died more selectively. GNP-anti integrin ${\alpha}_2$, GNP-anti EGFR with air plasma could be treatment choice of oral SCC.

• Asian Pacific Journal of Cancer Prevention
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• 제13권11호
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• pp.5455-5461
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• 2012

Luteolin is a plant flavonoid which exhibits anti-oxidative, anti-inflammatory and anti-tumor effects. However, the antiproliferative potential of luteolin is not fully understood. In this study, we investigated the effect of luteolin on cell cycling and apoptosis in human esophageal squamous carcinoma cell line Eca109 cells. MTT assays showed that luteolin had obvious cytotoxicity on Eca109 with an $IC_{50}$ of $70.7{\pm}1.72{\mu}M$ at 24h. Luteolin arrested cell cycle progression in the G0/G1 phase and prevented entry into S phase in a dose- and time-dependent manner. as assessed by FCM. Luteolin induced apoptosis of Eca109 cells was demonstrated by AO/EB staining assay and annexin V-FITC/PI staining. Moreover, luteolin downregulated the expression of cyclin D1, survivin and c-myc, and it also upregulated the expression of p53, in line with the fact that luteolin was able to inhibit Eca109 cell proliferation.

• 한국전자파학회논문지
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• 제16권7호
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• pp.681-689
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• 2005

본 논문에서는 IEEE 802.15.3a의 초광대역(Ultra Wideband: UWB) 시스템용 직접 변환 혼합기를 설계 및 제작하였다. 직접 변환 방식을 사용하는 UWB 혼합기는 dc offset, 2차 고조파 왜곡 등을 발생시키는데, 이 문제를 해결하기 위해 역병렬 다이오드 쌍을 이용하였다. 본 논문에서는 $3.1\~4.8$ GHz 동위상 전력분배기와 $1.5\~2.4$ GHz 광대역 $45^{\circ}$ 위상 분배기 설계를 위하여 결합선로를 이용한 새로운 월킨슨 분배기를 제안하였다. 역병렬 다이오드 구조와 광대역 소자를 이용한 혼합기의 제작 결과는 주파수 변환 손실이 약 18 dB, input third order intercept point($IIP_3$)는 약 15 dBm, 그리고 1-dB gain compression point($P_{1dB}$)는 2 dBm으로 측정되었다. I/Q 출력 양단 간의 전력오차는 0.5 dB, 그리고 위상오차는 ${\times}3^{\circ}$를 지닌 초광대역 쿼드러쳐 혼합기로 동작하였다.

• Journal of Microbiology and Biotechnology
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• 제30권8호
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• pp.1214-1221
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• 2020

Esculetin 6-O-β-D-arabinofuranosyl-(1 → 6)-β-D-glucopyranoside (EAG) is a coumarin glycoside isolated from the stem bark of Fraxinus rhynchophylla. This study scrutinized the anti-proliferative activity of EAG on blood cancer-derived Jurkat leukemic cells. Cell viability assays in leukemic cancer cells determined that EAG possesses potent anti-proliferative effects. Moreover, treatment with EAG increased the proportion of apoptotic cells, resulted in cell cycle arrest being induced at the subG0/G1 phase, and reduced the proportion of cells present in the S phase. In addition, mitochondrial membrane potential was reduced by EAG in Jurkat cells. Additionally, EAG triggered apoptosis that was mediated by the downregulation of BCL-XL, p-IκBα, and p-p65 expressions in addition to the upregulation of cleaved Caspase 3 and BAX expressions. These findings revealed that the toxic effect of EAG was mediated by intracellular signal transduction pathways that involved a mechanism in which reactive oxygen species (ROS) were upregulated. Thus, this study concludes that EAG could potentially serve as a therapeutic agent for leukemia.

• 대한한방부인과학회지
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• 제28권2호
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• pp.26-39
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• 2015

Objectives : Dendropanax morbifera is known as a tree that has been used in traditional medicine for various diseases. However, its biological activities in cancer have not yet been clearly elucidated. In this study, we investigated the anti-cancer effects of water extract of Dendropanax morbifera (DP) on 2 human breast cancer cell lines (estrogen dependent MCF-7 and estrogen independent MDA-MB-231). Methods : The MTT assay and flow cytometry were used to assess cell proliferation, along with cell cycle analysis. Nitric oxide production was detected by Griess assay. The expression of apoptosis related gene was assessed by quantitative real-time PCR. Results : Our data revealed that DP inhibits the cell growth in a dose dependent manner (0, 50, 100, 250, and 500 μg/ml) of both estrogen independent MDA-MB-231 and estrogen dependent MCF-7 breast cancer cells. Also, LPS induced nitric oxide production was significantly reduced by DP. Cell cycle analysis showed an increased G1 phase in the MCF-7 cell and G2/M phase in the MDA-MB-231 cell. DP decreased mRNA expression of apoptotic suppressor gene Bcl-xL, and increased mRNA expression of pro-apoptotic genes. DP increased mRNA expression of p21, and Rip1 in both cell. And DP decreased mRNA expression of survivin in the MCF-7 cell. Conclusions : Taken together, these results indicate that DP extract are source of anti-cancer potential and could be developed botanical drug.