• Applied Biological Chemistry
• /
• 제42권4호
• /
• pp.310-316
• /
• 1999

대두가공제품 중에 존재하는 Bowman-Birk protease inhibitor(BBPI) 함량을 protease 저해활성 측정 및 경합 Enzyme-Linked Immunosorbent Assay(ELISA)로 살펴보았다. 항체제조를 위한 BBPI는 ion exchange chromatography와 전기영동 후 gel slicing 방법으로 시판 soybean trypsin-chymotrypsin inhibitor로부터 순수, 분리하였다. 순수분리한 BBPI를 면역원으로 rabbit anti-BBPI antibody를 조제하였으며 단백질 농도별 titration방법으로 BBP에 비교적 선택적으로 결합하는 항체임을 확인하였다. 이를 이용한 경합 ELISA 방법으로 BBPI를 정량하기 위한 표준 정량곡선을 작성하였으며 시료용액중의 BBPI 함량이 $0.03{\sim}30\;{\mu}g/ml$ 범위일 경우에 정량적인 분석이 가능하였다. 대두품종별 chymotrypsin 저해활성은 $8,462{\sim}12,428\;U/g$이었으며 BBPI 함량은 $482{\sim}692\;mg%$ 이었다. 시판 대두 가공제품 중에서 5종의 콩나물은 건물량 기준으로 $10,695{\sim}13,249\;U/g$의 chymotrypsin 저해활성과 $529{\sim}803\;mg%$의 BBPI 함량을 나타내었으며 일부 두부제품에서도 68.9 mg%정도의 BBPI가 검출되었다. 그 밖의 두유, 된장, 고추장 및 간장 등의 대두발효식품, 탈지대두박 등에서는 chymotrypsin 저해활성 및 BBPI가 거의 검출되지 않았다.

• 한국식품위생안전성학회지
• /
• 제28권4호
• /
• pp.367-375
• /
• 2013

해동피는 약리학적으로 진통, 소염작용이 있어, 허리와 무릎의 통증 등을 치료하는데 효과가 있다고 알려져 있다. 본 연구에서는 해동피(Kalopanax pictus)의 국산 및 중국산과 그 유사생약으로 사용되는 절동피(znthoxylum ailanthoides)의 국산과 중국산, 목면피(bombax malabaricum)의 $H_2O$ 및 70% EtOH 추출물의 항위염 효과와 항산화효과에 대해 알아보았다. 먼저 HCl EtOH로 유발한 흰쥐위염 모델을 통해 효과를 확인하였고, 위염 및 위궤양, 위암의 원인으로 잘 알려진 H. pylori에 대한 colony 억제작용과 대표적 공격인자인 과다한 산에 대한 억제작용을 확인 하고자 제산력 시험법을 실시하였다. 항위염 효과에 대한 항산화 기전으로는 DPPH 시험법, 환원력 시험법, ${\beta}$-carotene/linoleic acid syetem을 이용한 지질과산화 억제활성 시험을 실시하였고, 실험에 사용한 추출물 중 가장 효과가 우수한 해동피 국산 추출물을 이용하여 superoxide radical 및 hydrogen peroxide 소거 작용을 확인하였다. Indomethacin으로 유발한 흰쥐 위궤양 모델에서는 해동피 국산 $H_2O$ 및 70% EtOH 추출물에서 각각 73.1%, 53.5%로 손상을 억제시킴을 확인하였다. 흰쥐를 이용한 gastric secretion 실험으로는 해동피 국산 $H_2O$ 및 70% EtOH 추출물이 위액의 분비를 줄이고, pH를 상승 시켰으며 총 산도를 감소시킴을 확인하였다. 그 결과, 해동피국산 및 중국산, 절동피국산 및 중국산, 목면피중국산 해동피의 항산화 효과가 가장 우수하였으며 해동피 국산은 위염 및 위궤양에서 우수한 효과를 확인하였고, 건강 기능성 식품 및 천연물 의약품으로서의 개발 가능성을 확인할 수 있었다. 또한, 이 결과를 토대로 오랜 세월 한약재로 사용되어 온 해동피의 기원이 다른 유사품 및 불량약재의 무분별한 유통, 정확한 기원의 약재에 대한 기준 설정, 높아지는 한약재 수입 의존도에 따른 위품, 저질품의 혼입 방지와 객관적, 신뢰성 있는 표준규격 설정에 기여할 것으로 예상된다.

• 대한화장품학회지
• /
• 제35권1호
• /
• pp.19-25
• /
• 2009

피부주름 개선에는 콜라겐(collagen)뿐만 아니라 탄력섬유인 엘라스틴(elastin) 등도 기여하는 것으로 보고되고 있다. 더 나아가 자외선에 의하여 사람의 피부에서 광노화 현상이 나타나며 자외선 조사 후 엘라스타제(elastase)의 활성이 증가하기 때문에 엘라스타제의 활성증가는 자외선에 의한 피부 탄성도의 감소 및 주름 생성의 주요원인으로 생각된다. 따라서 본 연구에서는 피부주름 생성에 영향을 미치는 엘라스타제의 활성을 측정하기 위한 모델을 마련하기 위하여 시판되는 두 가지 엘라스타제, 돼지 췌장 엘라스타제(porcine pancreatic elastase)와 사람 호중구 엘라스타제(human neutrophil elastase)를 사용하였으며 다음 세 가지는 정상 사람 섬유아세포(normal primary human fibroblasts), 쥐의 3T3 섬유아세포주(3T3 mouse fibroblasts), 사람의 CCD-25Sk 섬유아세포주(CCD-25Sk human fibroblasts)로부터 elastase를 준비하여 사용하였다. 준비된 5가지 효소의 농도에 기질의 농도 및 배양시간에 따르는 효소의 활성을 비교 평가하였다. 양성대조군으로 사용한 phosphoramidon이 normal human primary fibroblast elastase와 CCD-25Sk fibroblast elastase의 활성을 유의성 있게 억제한 반면에 porcine pancreatic elastase에는 별다른 영향을 미치지 못하였다. 따라서 주름 개선 후보물질 탐색을 위한 엘라스타제의 선정에는 신중한 고려가 필요할 것으로 사료된다.

• Archives of Pharmacal Research
• /
• 제25권6호
• /
• pp.903-909
• /
• 2002

Research in the cytokine field has grown exponentially in recent years, and the validity of such studies relies heavily on the appropriate measurement of levels of cytokines in various biological samples. Transforming growth factor (TGF)-$\beta$, a hormonally active polypeptide found in normal and transformed tissue, is a potent regulator of cell growth and differentiation. The most widely used bioassay for TGF-$\beta$ is the inhibition of the proliferation of mink lung epithelial cells. Though detection of [$^3$H]thymidine incorporation is more sensitive than the MTT assay, it presents some disadvantages due to the safety and disposal problems associated with radioisotopes. In this study, we attempted to ascertain the experimental conditions which could be used for measuring the in vitro biological activity of TGF-$\beta$ in a safer and more sensitive way compared with the currently available methods. We compared the commonly used method, the MTT assay, to the XTT assay using different parameters including cell number, incubation time and the wave length used for detecting the product. We examined the anti-proliferative activities of TGF-$\beta$ in three different cell lines: Mv-1-Lu mink lung epithelial cells, MCF10A human breast epithelial cells and H-ras-transformed MCF10A cells. Herein, we present an experimental protocol which provides the most sensitive method of quantifying the biological activity of TGF-$\beta$, with a detection limit of as low as 10 pg/ml: Mv-1-Lu or H-ras MCF10A cells ($1{\times}10^5/well$) were incubated with TGF-$\beta$ at $37^{\circ}C$ in a humidified $CO_2$ incubator for 24 hr followed by XTT treatment and determination of absorbance at 450 or 490 nm. Our results may contribute to the establishment of an in vitro bioassay system, which could be used for the satisfactory quantitation of TGF-$\beta$.

• 한국철도학회:학술대회논문집
• /
• 한국철도학회 2011년도 정기총회 및 추계학술대회 논문집
• /
• pp.2090-2100
• /
• 2011

Railway rail fastening system is the critical device which gives big influences to not only the vehicle driving stability and the orbit's structural stability against the impulsive load, but also the noise vibration and the ride comfort. As a part of the low-carbon green growth, the importance of the railroad industry is getting highlights on its excellent energy-efficiency and eco-friendliness. However, so far the Korea's domestic rail fastening system technology is not so good and the technical reliance to abroad is very heavy. In this study, we conducted comparative analysis on the rail fastening system with new and used one of the same type. And those systems are imported by Seoul Metro and are being used by it. With this basis, we developed the components and the materials and then, established the durability assessment methods appropriate to the Korean domestic circumstances. And through the reliability qualification test on the 7 parts of the rail fastening system, we've improved the reliability and guaranteed the 15 years of service lifetime. ($B_{10}Life15$) Establishment and standardization of Reliability Standard on the parts of the rail fastening system such as anti-vibration pads, guide-plate, screw spike made it possible to perform the internationally fair assessment. And it is thought that we can satisfy the manufactures' and consumers' needs of cost-cutting and qualification security by shortening of assessment period on rail fastening system.

• Asian-Australasian Journal of Animal Sciences
• /
• 제13권6호
• /
• pp.845-855
• /
• 2000

As high as 50% of pregnancies are known to fail and the majority of such losses occur during the peri-implantation period. For the establishment of pregnancy in mammalian species, therefore, implantation of the conceptus to the maternal endometrium must be completed successfully. Physiological events associated with implantation differ among mammals. In ruminant ungulates, an elongation of the trophohlast in early conceptus development is required before the attachment of the conceptus to the uterine endometrium. Moreover, implantation sites are restricted to each uterine caruncula where tissue remodeling, feto-maternal cell fusion and placentation take place in a coordinated manner. These unique events occur under strict conditions and are regulated by numerous factors from the uterine endometrium and trophoblast in a spatial manner. Interferon-tau (IFN-${\tau}$), a conceptus-derived anti-Iuteolytic factor, which rescues corpus luteum from its regression in ruminants, is particularly apt to play an important role as a local regulator in coordination with other factors, such as TGF-${\beta}$, Cox-2 and MMPs at the attachment and placentation sites.

• International Journal of Costume and Fashion
• /
• 제10권1호
• /
• pp.67-79
• /
• 2010

Cultural sensation by appearance of Elvis Aaron Presley is thought to have been greatly influenced by spread of popular culture beginning to form with the development of mess media in USA that was changed rapidly after the war, changing aspect of popular music in U.S.A through the media development and teenager culture of USA that has rapidly emerged. Elvis intended to change into 'music to be seen' deviating from 'music to be simply listened to' with expressing the conversion of sexual consciousness, defiance to older generation and a sense of value on wealth, and the like, and also suggested a new culture and style to the young people at that time. Flow of Elvis's fashion may be categorized into the classifications such as the fashion in the period. First, Formative period(before 1954) distinguished by pimp look and sideburns with tough image and wild clothes. Second, Establishment period(1954-57) may be categorized into the hot rockabilly style which showed a glittering fashion by brilliant and colorful costumes, the cool rockabilly style which changed him to a fashionable star of modem image(1958-60). Third, re-heyday(after 1968) where a brilliant and bold decoration was displayed. In Elvis fashion, the masculine sensualism, defiance to main value and expression of luxury and consuming culture, etc. are included as a unique aesthetic consciousness. 'Expression of masculine sensualism' represented the sensualism through sexual objectification of men's body by men's sexual ostentation which was prohibited. 'Defiance to main value' means that attempts of non-mainstream image for social class are expressed with an exercise of power by young people through the public. Although the Elvis look has fanned an anti-culture, it was expressed and accepted as having a normally capitalistic appearance. In addition, it being thought of as the standard, symbolizing a success and a wealth through the presentation of image which accepted this material benefit, we may classify this into 'expression of luxury and consuming culture'.

• BMB Reports
• /
• 제47권5호
• /
• pp.274-279
• /
• 2014

Bortezomib has been known as the most promising anti-cancer drug for multiple myeloma (MM). However, recent studies reported that not all MM patients respond to bortezomib. To overcome such a stumbling-block, studies are needed to clarify the mechanisms of bortezomib resistance. In this study, we established a bortezomib-resistant cell line (U266/velR), and explored its biological characteristics. The U266/velR showed reduced sensitivity to bortezomib, and also showed cross-resistance to the chemically unrelated drug thalidomide. U266/velR cells had a higher proportion of CD138 negative subpopulation, known as stem-like feature, compared to parental U266 cells. U266/velR showed relatively less inhibitory effect of prosurvival NF-${\kappa}B$ signaling by bortezomib. Further analysis of RNA microarray identified genes related to ubiquitination that were differentially regulated in U266/velR. Moreover, the expression level of CD52 in U266 cells was associated with bortezomib response. Our findings provide the basis for developing therapeutic strategies in bortezomib-resistant relapsed and refractory MM patients.

• 복식
• /
• 제37권
• /
• pp.71-88
• /
• 1998

This study will analyze the aesthetic qual-ities revealed in the work of Vivienne Wes-twood in order to gain an understanding of the development process and uniqueness of modern avant-garde fashion. Westwood gained worldwide recognition in the 1970s as the Punk movement emerged in London. Although her works have often been described as decadent and anti-establishment, her anarchic view of fashion has had a considerable influence on other designers, both in England and around the world. Vivienne Westwood's works can be divided into three periods. In the first period(1971∼78). Westwood design demonstrated elements which were variations of the subcultural Punk style. The noteworthy designs of this period included ripped T-shirts, bondage clothes, and fetishist accoutrements. In the second period(1979∼83), her designs expressed elements from ethnic and primitive tribal societies through the 'Pirate', 'Savages', 'Buffalo Girls', 'Witches' collections, which inspired New Romanticism movement. In the third period (1985∼ ), through works such as 'Mini-Crini'collection, her works identified elements from the old west and used materials such as crinoline and Harris tweed and contemporized them by rearranging the innovative technique. In the end the news synthesis helped formulate new ideas. The aesthetic qualities in Vivienne Wes-twood's works can bed identified with the following themes : Punkature, Erotic Intelligent-sia, Anarchic Collage. First, within Punkature, Westwood's ideas are at the forefront as her impact on the cut of clothes and creative detailing have been considerable. Also, while her ideas can be extreme, her clothes are wear-able, resulting in the synthesis of Punk and couture. Second, as Erotic Intelligentsia, Vivienne Westwood does not present sexuality as a straight forward attribute that fashion so often tends to do, but instead as a matter for inquiry, exploration and debate. She asserts that sexuality is always an interplay between the polarities of masculine and feminine, of dress and undress. Third, as Anarchic Collage, she has taken, juxtaposed, and transformed objects and symbols from dominant culture, like every modern subversive movement from Dada to Punk. She has continued this form of anarchic collage, mixing styles from various times and places, taking them out of their traditional context. From thiss viewpoint, Vivienne Westwood's works can be considered to be made up of past themes merged with is contemporary. The usage of contrasting elements such as Punk and couture to communicate her ideas visually or technically.

• Toxicological Research
• /
• 제23권3호
• /
• pp.231-238
• /
• 2007

Evaluation of potentials of chemicals to alter expression of genes that are involved in carcinogenesis may serve useful tools in toxicological research. In this investigation, we developed reporter cell lines that expressed luciferase in response to transactivation of hypoxia inducible factor-1, P53 tumor suppressor and Nur77 of which roles have been well established in cancer development and progression. Whereas these reporter cell lines displayed low constitutive backgrounds, the reporter activities were significantly enhanced in response to $desferriosamine/CoCl_2$, adriamycin or 6-mercaptopurine, which are hypoxia mimicking chemicals, P53 activator or Nur77 inducer, respectively. The activation of the reporter was time- and dose-dependent. Known tumor initiators and promoters, such as phorbol 12-myristate 13-acetate and phorbol 12, 13-dicaprinate induced the reporter activity at as low as 10nM in these stable cell lines. Further, known anti-tumor promoters, such as ascorbic acid and ${\beta}-carotene$ repressed the reporter activities. These results indicate that our stable reporter cell lines could serve as a useful system for rapid assessment of carcinogenicity of toxic chemicals.