• Journal of Evidence-Based Herbal Medicine
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• v.2 no.2
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• pp.1-7
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• 2009

Pulsatillae Radix, the root of Pulsatilla koreana Nakai (Ranunculaceae), has been traditionally used as a remedy for leukorrhea and amoebic dysentery by taking internally‘ and as an antimicrobial for external use. We carried out this study to examine the anti-dandruff effect of 70% Ethanol-Extract of Pulsatillae Radix (EPX) compared with 1 % zinc pyrithione used commercially as anti-dandruff. The EPX showed significant antimicrobial activity against Pityrosporum ovale, major cause of dandruff, and also showed significant anti-dandruff and itch-improvement effect of head skin.

• Journal of Physiology & Pathology in Korean Medicine
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• v.23 no.5
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• pp.1125-1131
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• 2009

Lophatheri Herba, Patriniae Radix, Alli tuberosi Herba, and Menthae Herba have long been used externally for treatment of injuries or inflammations in Korea. This study was carried out to examine the anti-dandruff effect of the mixture made with equal parts of 70%-ethanol extracts of Lophatheri Herba, Patriniae Radix, Allii tuberosi Herba, and Menthae Herba (LPAM) and of each one above, compared with 1%-zinc pyrithione (Standard) used commercially as anti-dandruff. The results showed the mixture was more effective than each crude drug. The 10%-LPAM produced no toxicity in skin reaction test and eye irritation test, and showed significant antimicrobial activity against Pityrosporum ovale, the major cause of dandruff, and also showed significant anti-dandruff and itch-improvement effects on head skin.

• Journal of the Korean Society of Fashion and Beauty
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• v.3 no.3 s.3
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• pp.31-40
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• 2005

The reason that aromatherapy began to draw attention again as the alternative therapy is because the essential oils that are used for aromatherapy not only allow the human body to maintain homeostasis but also have an effect on curing a mental feeling of fatigue and because these efficacies and effects are proceeding with being proved scientifically. Also, with coming to be high in a voice of concern about severally side effects that the modern medicine and the synthetic chemistry have, the aromatherapy came to be paid attention as the replacement therapy that can substitute for and supplement this. In particular, at the time of requiring a study on a substance to substitute for this after having been announced in Japan for 1998 a risk of Zincpyrithione that is being used as a component of dandruff treatment, it is thought that the aroma oil that was known for having an anti-dandruff effect has a merit of being easy in a user's access, thus it will be effective in curing the symptom of dandruff. Accordingly, this study conducted the following experiment in a bid to examine whether the aroma oil has the anti-microbial effect on the actual malassezia. First of all, it measured the minimum concentration of aroma oil in which the growth of malassezia is inhibited, by carrying out MIC(Minimum Inhibition Concentration) test in terms of anti-microbial activity against Malassezia furfur KCTC 7744 while using oils of Patchouli, Rosemary, Lavender, and Tea tree among aroma oils that are being used for dandruff treatment. As a result of that, Tea tree oil has the most excellent anti-microbial activity, and the measurement result of anti-microbial activity was shown in order of Lavender oil>Rosemary oil>Patchouli oil. Next, it carried out the is actually eased by applying Tea tree oil directly to the scalp of dandruff. In consequence of the experiment, the massage of Tea tree oil indicated the result of relieving the symptoms of dandruff such as scale, itch, pain, inflammation, and sebum, and among these symptoms of dandruff, it was shown to be most effective in easing itch. Especially, this clinical experiment was indicated that the massage of Tea tree oil has the most effect on relieving itch of targets with a dry-skin type who had no experience of dandruff treatment.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.34 no.3
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• pp.209-215
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• 2008

Iodopropynyl butylcarbamate and climbazole as anti-dandruff agents widely used in cosmetics and pharmaceutics were micronized using supercritical fluid. Supercritical carbon dioxide was selected due to relatively low critical temperature and critical pressure. Iodopropynyl butylcarbamate and climbazole were chosen because of their solubility in supercritical carbon dioxide. The rapid expansion of supercritical solution (RESS) experiments involved investigations of the effect of pressure, temperature on particle size and morphology.

• International Journal of Internet, Broadcasting and Communication
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• v.14 no.3
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• pp.171-181
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• 2022

Purpose: In this paper, we show our blending ratio of 10 types of Essential Oils that survives beneficial bacteria and kills harmful bacteria in the scalp, and we investigate the possibility of application of our blending ratio to the development of anti-dandruff products and the possibility of being used as a raw material for clinical beauty and customized cosmetics. Methods: The scalp microorganisms used in our study were M. furfur, S. epidermidis, E. coli, and P. nitroreducens. There are a total of 10 Essential Oils such as True Lavender, Lime, Roman chamomile, Rosemary camphor, Cedarwood, Geranium, Clove, Tea tree, Palmalosa, and Peppermint. The antibacterial test of the blended Essential Oil was carried out according to the test method of the standardized evaluation methodology of "Food and Food Additives Code". Since M. furfur is related to the growth of sebum in the scalp, in this study we used the fnLNB and the fnLNA with 20 ㎖ of whole fat cow milk added. Results: The blending ratio of EO, which inhibits dandruff-causing bacteria such as M. furfur, S. epidermidis, E. coli, and does not inhibit P. nitroreducens showing dominant growth in a healthy scalp, was B8(Clove 0.2%, Roman chamomile 0.5%, Tea tree 0.3%), B9(Geranium 0.1%, Palmarosa 0.1%, Roman chamomile 0.5%, Tea tree 0.3%), B10(Clove 0.1%, Geranium 0.1%, Palmarosa 0.1%, Roman chamomile 0.5%, Tea tree 0.2%). Conclusion: It is thought that the blending ratio of BEO obtained as a result of this study can provide a basis for use as an alternative to antibiotics in developing anti-dandruff drugs and emerge as a new alternative to solve scalp microbial imbalance. In order for EO to be used as a useful raw material for anti-dandruff preparation, researches on 1) Standardization (the effects of products differ according to the types, regions, climate, extraction methods, etc.), 2) Antimicrobial effects, 3) Safety, etc., must be established.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.25 no.3
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• pp.47-66
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• 1999

We have tried to measure the anti-dandruff effect of the several kinds of formulations by determining the MIC values of the P. ovale which was determined by resazurin(alarmar Blue$^{TM}$). To get high reproducibility, it was suggested that about 2.6$\times$10$^{5}$ cfu/$m\ell$ of P ovate should be incubated with alarmar Blue$^{TM}$, optimum dilution ratio between alarmar Blue$^{TM}$ and PBS buffer should be 1:1 -1:4, and optimum incubation time should be 16 ~ 24 hours. Even though 1:1 diluted alarmar Blue$^{TM}$ was incubated with P ovale, the metabolic activity of if ovule was not inhibited by alarmar BlueTM. The Minimum Inhibitory Concentration(MIC) values of several kinds of anti-dandruff formulation which were the mixture system between Zinc pyrithione and Climbazole make it possible to determine the optimal anti-dandruff formulation, which show similar results with that of microscopic MIC determination and that of SDDM(Skin-Disk Diffusion Method). It is expected that the anti-dandruff test method which uses alarmar Blue$^{TM}$ could be used as an effective in vitro test method because it was not so much affected by the turbidity of the broth and samples, and it can afford the MIC values of many samples within relatively short time by using microplate reader.te reader.

• Toxicological Research
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• v.26 no.1
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• pp.47-52
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• 2010

The Malassezia fungi are responsible for various human skin disorders including dandruff and seborrheic dermatitis. Of the Malassezia fungi, Malassezia globosa (M. globosa) is one of the most common in human scalp. The completed genome sequence of M. globosa contains four putative cytochrome P450 genes. To determine the roles of Malassezia P450 enzymes in the biosynthesis of ergosterol, we isolated MGL3996 gene from M. globosa chromosomal DNA by PCR. The MGL3996 gene encodes an enzyme of 616 amino acids, which shows strong similarity with known CYP52s of other species. MGL3996 gene was cloned and expressed in Pichia pastoris (P. pastoris) heterologous yeast expression system. Using the yeast microsomes expressing MGL3996 protein, a typical P450 CO-difference spectrum was shown with absorption maximum at 448 nm. SDS-PAGE analysis revealed a protein band of apparent molecular weight 69 kDa and Western blot with anti-histidine tag antibody showed that MGL3996 was successfully expressed in P. pastoris. Cloning and expression of a new P450 gene is an important step to study the P450 monooxygenase system of M. globosa and to understand the role of P450 enzymes in pathophysiology of dandruff.

• Journal of Mycology and Infection
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• v.23 no.4
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• pp.91-98
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• 2018

Background: Malassezia, a lipophilic yeast, is a causative agent for dandruff and seborrheic dermatitis. Many biological agents have been studied for anti-Malassezia effect but further studies are needed for their clinical application. Objective: The study was conducted to evaluate the inhibitory effect of different natural essential oils and a fruit extract on Malassezia species in an in vitro study and a clinical trial. Methods: The antifungal effects of natural essential oils and a fruit extract on Malassezia species (M. furfur and M. sympodialis) were evaluated by measuring the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) and using the disc diffusion method. Natural essential oils of citron seed, lavender, and rosemary and citrus junos fruit extract were used for the in vitro study. The clinical trial was conducted with a shampoo containing four ingredients. A total of 22 subjects used the shampoo every day for 4 weeks and were evaluated using clinical photography, trichoscopy, and sebumeter at baseline, 2 weeks, and 4 weeks after treatment. Results: Antifungal activity of agents was relatively lower in lavender and rosemary essential oils at MIC and MFC. Disc diffusion method revealed same results. In the clinical trial, the amount of sebum decreased statistically significantly and erythema, dandruff, and lesion extent also improved. Conclusion: The natural essential oils and fruit extract are effective for suppressing Malassezia activity, therefore these might be used as an alternative for treatment of dandruff and seborrheic dermatitis.

• Journal of Haehwa Medicine
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• v.20 no.2
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• pp.67-78
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• 2012

Volatile odor substance originating from drying and making dry peel of tangerine from the fruit skin were collected through modern equipment. The anti-microbial activity of the substance against various skin-residing bacteria including acne, dandruff, athelete's foot, and gingivitis inducing microorganisms were tested. Anti-microbial activity was observed in purified oil, where 87 to 92% was D-limonene. Against P. acnes, 103$cfu/m{\ell}$ of P. acnes were suppressed at 0.1% Unshiu oil, and the MIC was measured to be 0.3%. Against P. ovale, a dandruff inducing bacteria, 104$cfu/m{\ell}$were suppressed at 0.1% Unshiu oil, and the MIC was measured to be 0.1%. Against T. rubrum and T. Mentagrophytes, both of which are athelete's foot inducing microorganisms, 83% of T. Mentagrophytes and 99.9% of T. rubrum were suppressed at 0.1% Unshiu oil, and the MIC were 0.3% and 0.05% respectively. Against S. aureus, a skin infection inducing bacteria, 103$cfu/m{\ell}$ of the bacteria were suppressed at 0.1% Unshiu Oil. Against B. subtilis, a non-pathogenic sporulating bacteria, 104$cfu/m{\ell}$ of the bacteria were suppressed at 0.1% Unshiu Oil. Against C. albicans, found in mucous membranes, 104$cfu/m{\ell}$ of the bacteria were suppressed at 0.1% Unshiu Oil. Against Aspergillus niger, an otomycosis inducing microorganism, 99.9% were suppressed at 0.1% Unshiu Oil. The results above indicate that low concentration of purified oil extracted from tangerine had strong antimicrobial activities against bacteria and fungi residing on the skin, and that it may be developed into skin disorder treating products in the future.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.24 no.1
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• pp.100-112
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• 1998

향 비듬 샴푸에 대한 비중이 증가되는 현 샴푸시장에서 향 비듬력을 측정할 수 있는 간단한 in vitro 실험법의 요구가 증대되고 있다. 기존의 향 비듬력의 측정을 위하여 사용되고 있는 방법으로는 Pityrosporum ovale에 대한 MIC test와 halo test가 널리 쓰이고 있으나, Pityrosporum 배지의 자체 탁도 때문에 균의 성장 여부를 육안으로 관찰하기 어렵고, 종이 디스크와 두피와의 차이에 의하여 실제 사용과 효과가 다르다는 단점이 있었다. 저자들은 임상 실험을 거치기 전 간단히 향 비듬력을 측정할 수 있는 in vitro 방법으로 skin disk diffusion method를 개발하였다. SDDM에서는 종이 디스크 대신 기니픽의 피부 조직을 사용하고, 향 비듬 제제의 처리 후 세척 단계를 포함시켜 실제 사용과의 차이를 줄였다. SDDM의 유용성을 확인하기 위하여 향 비듬 제제로 2.0% ketoconazole, 분산된 상태의 zinc pyrithion, 분자화 기술로 활성을 높인 ZPT에 대해 기존의 in vitro 시험법과 함께 향 비듬력을 측정하였다. 결과는 SDDM 시험에서만 20% ketoconazole 제제와 분자화된 0.5% ZPT제제의 억제대가 비슷한 것으로 나타나 임상 시험에서 두 제제 모두 비듬에 대해 개선 효과가 있었으며 향 비듬 효과에 차이가 없다는 결과와 일치하였다. 본 시험의 결과로 SDDM이 향 비듬 샴푸와 같은 wash-off 제품의 효능 측정에 간단하고 효과적인 적절한 in vitro 시험법으로 생각되어 SDDM 시험과 임상 시험결과를 소개한다.