• The Plant Pathology Journal
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• 제26권2호
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• pp.185-188
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• 2010

This study was undertaken to isolate and to identify a fungal pathogen Monilinia sp. KV-27 associated with apple anthracnose. Rotted Fuji apples were used for the isolation of the fungus. The infected tissues were sterilized with 70% ethanol, washed with sterilized distilled water and were transferred to 50 ml containing potato dextrose broth (PDB) flasks. The peripheral hyphae of the fungal colony which developed from the infected tissues were isolated on to potato dextrose agar (PDA). On PDA plates the fungus grew well at $25^{\circ}C$ and occupied more than half of a 9 cm petri dish within 5 days. The fungal cultures on PDA were used for morphological observation and identification of the fungus. Conidiophores were produced on the gray to whitish sporodochial structures scattered on PDA plates. These conidiophores gave rise to chains of conidia, which were branched and easily detached in water. These structures were dark brown to black and consisted of hyphal masses. Conidia produced on PDA plates were hyline or light colored, lemon shaped or ellipsoidal ($10-13{\times}8.5-11{\mu}m$) in size.

• 한국환경농학회지
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• 제28권4호
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• pp.442-446
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• 2009

This study was undertaken to isolate and to identify a fungal pathogen Colletotrichum sp. KV-21 associated with apple anthracnose. Rotted Gala apples were used for the isolation of the fungus. The infected tissues were sterilized with 70% ethanol, washed with sterilized distilled water and were transferred to 50 ml containing potato broth (PDB) flasks. The peripheral hyphae of the fungal colony which developed from the infected tissues were isolated on to potato dextrose agar (PDA). On PDA plates the fungus grew well at $25^{\circ}C$ and occupied more than half of a 9 cm petri dish within 5 days. The fungal cultures on PDA were used for morphological observation and identification of the fungus. Conidiophores were produced on the gray to whitish sporodochial structures scattered on PDA plates which gave rise to conidiogenous cells. The structures of the conidia produced on PDA plates were subcylindrical to obovoid, fusoid, tapered and 4 to $6\;{\mu}m$ in size.

• 한국산림과학회지
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• 제85권3호
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• pp.439-442
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• 1996

본 연구(硏究)는 조경수(造景樹)로 많이 식재(植栽)되고 있는 사철나무에 피해(被害)를 주어 탄저병 감염(感染)이 잘되고 조기낙엽현상(早期落葉現象)이 일어나 미관(美觀)을 크게 해치는 사철나무혹파리에 대한 생태(生態) 및 약제방제방법(藥劑防除方法)을 연구(硏究)한 결과이다. 1. 사철나무혹파리의 우화시기(羽化時期)는 4월하순(月下句)~5월하순(月下句)(춘천지역(春川地域))이며, 성충(成蟲)의 체장(體長)은 평균(平均) $1.88{\pm}0.43mm$, 성충(成蟲)의 수명(壽命)은 약 11시간(時間), 성충(成蟲)의 성비(性比)는 ♀ 56%, ♂ 44%, 포란수(抱卵數)는 평균(平均) $90{\pm}28.01$개(個), 알의 모양은 장타원형으로 평균(平均) 길이 $0.32{\pm}0.013mm$, 유충(幼蟲)의 길이는 평균(平均) $1.64{\pm}0.25mm$였다. 용화(蛹化) 기간(期間)은 3월중순(月中句)부터 시작되며 용기간(蛹期間)은 약 40~45일(日)로 당해(當該) 년도(年度) 춘기(春期) 기온(氣溫)에 따라 약간의 차이가 있다. 2. 방제농약(防除農藥)으로는 카보 3%입제(粒濟)를 피해목(被害木) 주변(周邊)에 $1m^2$당(當) 200g씩을 3월중순(月中句)에 1차 매립(埋立)하여 주고 성충(成蟲) 우화(羽化)시작 후 1주일 정도 지난 후에 2차(次) 매립(埋立)처리한 시험구(試驗區)에서 96.2% 이상(以上)의 구제효과(驅除效果)가 인정(認定)되었다.