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검색결과 141건 처리시간 0.024초

Nitric Oxide 화합물 첨가가 소 체외수정란의 체외발육에 미치는 효과 (Effects of Nitric Oxide Scavenger and Inhibitor on the Development of Bovine IVM/IVF Embryos)

  • 장현용;김종택;박춘근;정희태;김정익;양부근
    • Reproductive and Developmental Biology
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    • 제28권3호
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    • pp.161-166
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    • 2004
  • 체외수정 후 체외배양48시간에 난구세포의 제거 유ㆍ무에 따라 CR/sub 1aa/ 배양액에 homoglobin을 0, 1, 5 ㎍/㎖를 첨가한 구의 상실배기 이상 체외발육성 적은 난구세포를 제거한 구에서 23.8%, 33.3% 및 26.8% 였으며, 난구세포를 제거하지 않은 구에서는 각각 39.5%, 54.8% 및 48.8%로서 난구세포를 제거하지 않은 구에 Hb를 1 ㎍/㎖를 첨가한 구가 여타구보다 통계적으로 높은 결과를 얻었다(P<0.05). 체외수정 후 체외배양 96시간 후 난구세포를 제거 유ㆍ무에 따라 Hb를 0, 1, 5 ㎍/㎖를 첨가하였을 때, 상실배기 이상 체외발육성적은 난구세포를 제거한 구에서 각각 28.6%, 46.2% 및 39.1%였으며, 난구세포를 제거하지 않은 구에서는 각각 33.9%, 67.2% 및 46.0%로서, 난구세포를 제거하지 않은 구에 Hb를 1 ㎍/㎖를 첨가구가 여타구에 비해 통계적으로 유의하게 높게 나타났다(P<0.05). CR/sub 1aa/ 배양액에 L-NAME를 0, 10, 50 및 100 mM을 첨가한 구에서 상실배 이상 발육된 체외발육성적은 각각 55.6%, 64.9%, 58.8% 및 66.7%로써 L-NAME 첨가구가 무첨가구에 비해 커다란 차이가 나타나지 않았다(P>0.05). 모든 처리구에 배반포까지 발육된 체외수정란의 세포수에는 커다란 차이가 인정되지 않았다.

토끼의 바이러스성 출혈성 폐렴(잠정명칭) 발생 (An Outbreak of Viral Haemorrhagic Pneumonia(Tentative Name) of Rabbits in Korea)

  • 박남용;정치영;김진호;조성만;차연호;정병탁;김동성;윤지병;박진열;위성하
    • 대한수의사회지
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    • 제23권9호
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    • pp.603-610
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    • 1987
  • The pathological and microbiological studies were carried out to investigate an acute, febrile, highly fetal, infectious disease of rabbits that had occurred in the Winter and in the Spring and that had begun to be reported in Korea from November, 1985. The clinical signs of this disease were characterized by high fever, lethargy, piercing shriek, convulsion, and sudden death with epistaxis, but often they were not observed. The predominant pathogical findings were severe congestion and hamorrhage in trachea, dark brown discoloration of liver by diffuse necrosis or acute viral hepatitis, and hamorrhagic damages of lung, heart, spleen, kidney, etc. The etiological agent was a small round virus, in 25-35nm in diameter and without envelope, thus looking like a picorna virus. This disease resembled what was called the 'Viral Hamorrhagic Pneumonia in Rabbits'(tentative name) that had been reported for the first time in China in 1984. It will be desirable that the disease should be renamed as the 'Viral Hemorrhagic Fever in Rabbits', the 'Acute Viral Hepatitis in Rabbits', etc. because of its charateristics and the basis of pathological findings. An inactivated vaccine is now in the process of preparation for the prophylaxis of this viral disease.

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First Korean Record of Porcellanopagurus japonicus (Decapoda: Paguroidea), a Hermit Crab Living in Bivalve Shells

  • Jung, Jibom;Lee, Sang-Hui
    • Animal Systematics, Evolution and Diversity
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    • 제36권4호
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    • pp.391-395
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    • 2020
  • This study reports the pagurid hermit crab Porcellanopagurus japonicus in Korean waters for the first time. This species has a right cheliped larger than the left one, reduced and globular abdomen, and symmetrical uropods. Porcellanopagurus japonicus is similar to P. nihonkaiensis, a previously reported congeneric species in Korea, but also has distinguished morphological and ecological characters. There are currently issues with the Korean scientific name for P. japonicus, we suggest a reasonable Korean scientific name of this organism with its etymology. In addition, this species inhabits bivalve shells, which is not a typical habitat for hermit crabs, and the unique habitats of this and several other Korean hermit crabs are reviewed.

한(韓) 중(中) 일(日)의 공정서(公定書)(약전(藥典) 및 규격집(規格集))에 수재(收載)된 전통천연약물(傳統天然藥物)의 품질(品質)에 관한 조사(調査) (I) -기원(起源)에 관한 분류학적(分類學的) 검토(檢討)- (Systematic Botanical Survey of Traditional Herbal Medicines Listed in the Official Drug Compendia(Pharmacopoeia and Natural Drug Standards) of Korea, China and Japan)

  • 박상희;염정록;장일무
    • 생약학회지
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    • 제22권2호
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    • pp.112-123
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    • 1991
  • Traditional drugs(herbal and animal drugs) of Korea, China and Japan have developed essentially from the same origin, since the traditional medicine of three countries has been originated from ancient China. Due to different geographical locations and discrepancy of plant resources of the traditional drugs, some divergency in terms of systematic botany in traditional drug materials has appeared in the three countries. Present report aims to survey traditional herbal drugs that have been called same traditional names in three countries, but they are actually different with respect to systematic botanical view-point. The official drug compendia(pharmacopoeia and natural drug standards) of three countries were subject to examination. Survey was conducted by the following categories. Traditional drugs were listed under same name, however, 1) they belong to different genus; 7 traditional herbal drugs were listed. 2) they belong to same genus, but different species; 24 traditional herbal drugs were found. 3) a variety of related species are used; 15 traditional drugs were listed. 4) actually same plant, but taxonomical name is differently called and/or different parts of plant are used; 10 traditional drugs were counted. 5) animal drugs belong to one of the above categories; 7 traditional animal drugs were found. Total 63 traditional drugs(herbal and animal) were found to comprise different taxonomical names when the official drug compendia of Korea, China and Japan were examined.

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Nitric oxide 화합물 첨가가 한우 체외수정란의 체외발육에 미치는 효과

  • 장현용;박동헌;박춘근;정희태;김정익;양부근
    • 한국동물번식학회:학술대회논문집
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    • 한국동물번식학회 2001년도 춘계학술발표대회
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    • pp.88-88
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    • 2001
  • 소 난포란을 체외에서 성숙, 수정시킨 후, 2~8 세포기 수정란을 체외배양액인 CR$_1$aa에 일정량의 nitric oxide scavenger, Hb와, inhibitor인 L-nitro - L- arginine methyl ester (L-NAME)를 첨가 배양하여 체외발육에 미치는 영향을 검토하고자 실시하였다. 체외수정 후 체외배양 48시간에 난구세포의 제거 유.무에 따라 CR$_1$aa 배양액에 hemoglobin을 0, 1, 5$\mu\textrm{g}$/$m\ell$를 첨가한 구의 상실배기이상 체외발육성적은 난구세포를 제거한 구에서 23.8%, 33.3% 및 26.8%였으며, 난구세포를 제거하지 않은 구에서는 각각 39.5%, 54.8% 및 48.8%로써 난구세포를 제거하지 않은 구에 Hb를 1$\mu\textrm{g}$/$m\ell$를 첨가한 구가 여타구 보다 통계적으로 높은 결과를 얻었다(P<0.05). 체외수정 후 체외배양 96시간 후 난구세포를 제거 유.무에 따라 Hb를 0, 1, 5$\mu\textrm{g}$/$m\ell$를 첨가하였을 때, 상실배기 이상 체외발육성적은 난구세포를 제거한 구에서 각각 28.6%, 46.2% 및 39.1%였으며, 난구세포를 제거하지 않은 구에서는 각각 33.9%, 67.2% 및 46.0%로써, 난구세포를 제거하지 않은 구에 Hb를 1$\mu\textrm{g}$/$m\ell$첨가구가 여타구에 비해 통계적으로 유의하게 높게 나타났다( P<0.05).CR$_1$aa배양액에 L-NAME를 0, 10, 50 및 100mM을 첨가한 구에서 상실배이상 발육된 체외발육성적은 각각 55.6%, 64.9%, 58.8% 및 66.7%로써 L-NAME 첨가구가 무첨가구에 비해 커다란 차이가 나타나지 않았다(P>0.05). 본 실험의 결과로 볼 때, nitric oxide scavenger인 hemoglobin의 첨가는 체외수정 후 체외배양 96시간에 1$\mu\textrm{g}$/$m\ell$ 첨가하는 것이 체외수정란의 체외 발육율을 향상시켰으며, nitric oxide inhibitor인 L-NAME는 첨가 농도에 관계없이 체외수정란의 체외발육율에 영향을 미치지 않는 것으로 나타났다. 본 실험에서 생산된 배반포기 체외수정란의 세포수에는 커다란 차이가 인정되지 않았다.

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소 음경후인근의 Nitric oxide(NO) 매개성 이완 (Nitric oxide(NO)-mediated relaxation of bovine retractor penis muscle)

  • 양일석;장희정;강동묵;이장헌
    • 대한수의학회지
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    • 제36권3호
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    • pp.599-605
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    • 1996
  • This study was designed to examine the mechanism of penile erection in adult bull by analyzing the responses of bovine proximal retractor penile muscle strips(BRP) to electtical field stimulation(EFS), exogenous nitric oxide(NO), NO synthesis precursor(L-arginine), NO synthase inhibitors(L-NAME, L-NMMA), guanylate cyclase inhibitor(methylene blue) and nonspecific potassium channel blocker(tetraethylammonium, TEA) treatments. Isometric tension of BRP was measured using physiograph. Results were summarized as follows: 1. EFS of nonadrenergic noncholinrgic(NANC) nerve in BRP produced frequency-dependent inhibitory responses to the contraction induced by co-treatment of epinephrine, guanethidine and atropine. The inhibitory responses to EFS were blocked by tetrodotoxin(TTX, $1{\mu}M$). 2. Treatment of L-NAME ($10,\;20{\mu}M$) inhibited the relaxation to EFS whereas L-NMMA ($100{\mu}M$) had no effect. 3. Treatment of NO($20,\;40{\mu}M$; as an acidified solution of $NaNO_2$) induced concentration-dependent relaxation whereas preincubation of TTX($1{\mu}M$) and L-NAME($20{\mu}M$) had no effect on the relaxation response. 4. L-arginine treatment(10mM) blocked the inhibitory effect of L-NAME($20{\mu}M$). 5. Pretreatment of methylene blue($40{\mu}M$) reduced the NANC-induced relaxation of BRP. 6. Tetraethylammonium(TEA, 80mM) reduced NANC relaxation. These results suggest that NO may act as a NANC neurotransmitter in BRP and the effects might be mediated by cGMP and potassium channel.

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NOS만성억제로 인한 고혈압에서 아드레날린성 활성증가기전 (Mechanism of Increased Adrenergic Activities in Hypertension Induced by Chronic Inhibition of NOS)

  • 정국현;이석용
    • 약학회지
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    • 제45권1호
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    • pp.85-92
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    • 2001
  • Nitric oxide is a tonically produced vasodilator that maintains blood pressure in the normal animal. The chronic inhibition of nitric oxide synthase (NOS) elicits the hypertension in rats. However, the mechanism of hypertension induced by chronic inhibition of NOS is not clear. Thus, to clarify the mechanism of the occurance of hypertension, the changes in $\alpha$-adrenergic systems in rats treated with NOS inhibitors for 21 days were examined. Chronic administration of L-NAME significantly increased in the basal blood pressure, but chronic administration of 7-nitroindazole did not. Phenylephrine and G-protein stimulator elicited the more potent contraction in the aorta of the L-NAME-induced hypertensive rats. However when the contractile responses by phenylephrine and G-protein stimulator were calculated the proportion to the contraction by 25 mM KCL, there was no difference between the vehicle-treated rats and the L-NAME-treated rats. The density of $\alpha$-adrenergic receptors in aortic tissue was not changed by the chronic inhibition of NOS. These results suggest that hypertension induced by chronic inhibition of NOS is due to the inhibition of eNOS and the increased responses to the adrenergic drugs are due to the changes of the intracellular contactile mechanism of aortic tissue rather than the changes of receptor density.

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Taxonomic Review of Chrysis smaragdula Group (Hymenoptera: Chrysididae) from Korea

  • Jeong-Kyu Kim;Minkyu Jeong
    • Animal Systematics, Evolution and Diversity
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    • 제40권2호
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    • pp.150-156
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    • 2024
  • A taxonomic review of the Korean Chrysis smaragdula group (Hymenoptera: Chrysididae), characterized by the six apical teeth (including lateral angles or obtuse protuberances) of metasomal tergum 3, is presented. Three species, namely Chrysis daphne, C. equestris, C. principalis, are included herein. Of these, C. equestreis is recorded from Korea for the first time. Chrysis daphne is a corrected name for the previous Korean records under the name of C. fasciata. A determination key to species complemented with illustrations is made. Additional diagnoses including color variations and descriptions of male genitalia (unavailable in C. equestris) based on Korean materials are also provided.

N-{2-[2, 3,-bis-(methoxy-carbonyl) -guanidino]-5-(phenyl-thio)-phenyl}-2-methoxy-acetamid, Febantel(=Rintal)에 의한 가축의 장내기생충에 대한 구충실험 (Anthelmintic Efficacy of Febantel (Rintal) upon Intestinal Parasites of Domestic Animals)

  • 이원창;윤화중;정용서
    • 대한수의사회지
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    • 제16권2호
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    • pp.67-71
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    • 1980
  • In order to find out the anthelmintic efficacy upon intestinal parasites of domestic animals, a single dose of Rintal (generic name; Febantel) was administered orally against 10 heads of helminthes-infested dairy cattle and 21 heads of helmintesinfested s

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