• Molecules and Cells
• /
• v.25 no.3
• /
• pp.452-456
• /
• 2008

The interactions between monocytes and extracellular matrix proteins have been implicated in atherosclerosis pathophysiology. In the present study we evaluated monocyte attachment and migration through oxidized and non-oxidized collagen IV. Monocyte attachment was tested on microwells coated with either native or oxidized collagen IV. Monocyte migration through collagen IV was examined on transwells. Monocytes derived from patients with diabetes mellitus showed an increased ability to attach and migrate through collagen IV as compared to those derived from healthy volunteers. Moreover, control monocytes attached to oxidized collagen at a higher degree, while they migrated through oxidized collagen at a lower degree, as compared to the native protein. Our results also showed the involvement of the alpha2 integrin subunit in the above phenomena suggesting a modified interaction between monocytes and collagen IV in diabetes mellitus.

• Journal of Veterinary Clinics
• /
• v.37 no.1
• /
• pp.23-27
• /
• 2020

Between May and November 2018, babesiosis was examined in 162 bloods samples obtained to an animal hospital in Jeju island for anemia and medical examination. Sixty-two of 162 (38.3%) were positive by PCR. The ultra fast real-time PCR test with blood directly analyzed without DNA extraction showed the same results. Accurate diagnosis, treatment and prognosis of babesiosis should be combined with clinical symptoms, blood tests, the babesia antibody test, and the PCR antigen test. Ultra fast real-time PCR, with these tests, is expected to be a point-of-care testing (POCT) for easy, fast and accurate diagnosis of babesiosis in the veterinary clinic.

• Journal of Veterinary Clinics
• /
• v.37 no.3
• /
• pp.123-129
• /
• 2020

We aimed to investigate the differences in the efficacy of insert and circumaural earphones when performing the brainstem auditory evoked response (BAER) test with dogs. Hearing loss may occur congenitally or secondarily in dogs. The BAER test, unlike the classical ethological method, is the most reliable diagnostic tool to assess canine auditory function. Furthermore, there are certain advantages of using insert earphones rather than the standard, circumaural earphones. We subjected eight dogs to the BAER test with insert earphones and circumaural earphones. The result revealed that the latency of waves was delayed with an insert earphone. The inter-peak latency did not show any significant differences between the two transducers, and the threshold was higher when using an insert earphone. Moreover, the circumaural headphones produced a greater degree of crossover effect than the insert earphones, and this cross-over effect could affect the outcomes of the BAER test. Considering these results, we concluded that insert earphones may be more appropriate when performing the canine BAER test.

• Journal of the Korea Safety Management & Science
• /
• v.12 no.4
• /
• pp.73-80
• /
• 2010

The purpose of this study was to investigate the actual conditions of radiation safety supervision in animal clinics using inspection standard of X-ray generator for diagnosis. The surveys for inspection standard system, equipment condition, and safety supervision were carried out in 18 animal clinics randomly. The inspection standard included reproducibility of dose exposure, kVp, mAs, collimator accuracy test, collimator luminance test, X-ray view box luminance test, grounding system equipment test and external leakage current test. The surveys of equipment condition and safety supervision used one-on-one interview with 5 points measurement. As a result, 44.44% of reproducibility of dose exposure was proper, 81.25% of kVp test was good, and 100% of mAs test was appropriate. Also, 66.66% of collimator accuracy test was proper, 61.11% of collimator luminance test was good, 53.13% of X-ray view box luminance test was suitable. In addition, only 5.55% of grounding system equipment and ground resistance was proper, 63.64% of external leakage current test was appropriate in grounding system equipment test. The 100mA electric capacity of X-ray generator for diagnosis was popular with 44.44%, and its 55.56% was purchased used equipment. Monthly average of less than 50 times (61.11%) was top frequency in use, and no animal clinic had a thermo-luminescence dosimeter(TLD). The 16 animal clinics with radiation safety zone and 2 without radiation safety zone were appeared.

• Korean Journal of Veterinary Service
• /
• v.34 no.4
• /
• pp.403-407
• /
• 2011

This study describes a canine lymphosarcoma with a rapid proliferation and recurrence. A 4-year-old, male, Shih Tzu dog was examined for acute swelling mass. The mass had been identified since 3 months ago and enlarged $10{\times}7$ cm and located in the right axillary region. The surgical removal was recommended when patient visited veterinarian and the operation was conducted. The removed tumor was $11{\times}8{\times}7$ cm and firm, lobulated and white cut surface. Routine screening laboratory test was assessed with blood and radiological analysis. The metastasis sign was not detected on thoracic and abdominal radiography. Blood test revealed decreased lymphocytes. After surgical removal of the mass, microscopic histopathological examination was performed to determine the final diagnosis. Histopathologically, the tumors are characterized by the same histological features, including the presence of neoplastic cellular populations, and lymphocytes infiltration in varying proportions. Also, DNA was extracted and PCR analysis was employed to analyze the origin of tumor cells. T-cell specific nucleic acid fragments were specifically amplified by PCR. On the basis of the laboratory results, the tumor was diagnosed with canine T-cell lymphosarcoma. On the basis of our knowledge, this is the first report of canine T-cell lymphosarcoma in a Shih Tzu dog.

• Asian-Australasian Journal of Animal Sciences
• /
• v.21 no.12
• /
• pp.1779-1784
• /
• 2008

Seasonal changes in the concentration of free amino acids were determined monthly in plasma from the jugular blood of 50 thoroughbred racehorses that compete during the night between June and September and during the day between October and January. The concentration of most free amino acids remained relatively constant between June and January. However, those of glutamic acid, alanine, isoleucine and lysine tended to decrease and that of arginine (Arg) significantly decreased between July and September. The concentration of methionine (Met) gradually increased between June and September and significantly decreased thereafter. The concentration of 3-methylhistidine (3-MH) significantly increased between July and September and decreased thereafter. There were significant correlation between 3-MH and Arg. In conclusion, this study provided evidence of significant seasonal change in plasma 3-MH, Arg and Met of racehorses, and this was considered to relate to an environmental effect. Moreover, our study is the first to show 3-MH in plasma of racehorses affected by environmental change.

• Journal of Veterinary Clinics
• /
• v.22 no.4
• /
• pp.435-438
• /
• 2005

Two dogs (case 1:2-year-old intact male German Shepherd-mixed dog and case 2: 4-year-old intact female Jindo miked) with perineal hernia were referred to the Chonbuk Animal Medical Center, Chonbuk National University. In the both cases, there were unilateral perineal swelling involving the right side of the anus. In addition, in case 2, swelling was also present ventral to the anus. Rectal palpation of pelvic diaphragm revealed weakness of the muscles. Both cases were surgically treated using muscle transposition technique for hemiorrhaphy. In case 1, transposition of the superficial gluteal and internal obturator muscles and in case 2, transposition oi semitendinosus muscle was performed for hemiorrhaphy and reconstruction of the pelvic diaphragm. Both dogs became recovered after the surgical correction and no complications were observed during 10-month (casel) and 9-month(case2). Muscle transposition can be a useful technique for the treatment of perineal hernia in the dog.

• Journal of Veterinary Science
• /
• v.23 no.6
• /
• pp.83.1-83.7
• /
• 2022

Severe fever with thrombocytopenia syndrome (SFTS) is a zoonotic disease, and its clinical information and prevalence are important. This study was conducted on 22 feline patients from the Republic of Korea (ROK), suspected to suffer from a tick-borne disease. Four cats were positive for SFTS, and genotypes B-1, B-3, D, and F were identified. Clinical symptoms, such as anorexia, jaundice, thrombocytopenia, leukopenia, and hyperbilirubinemia, were detected. This is the first report of SFTS virus genotypes B-1, D, and F from cats in the ROK. Moreover, our results suggest that jaundice may be an indicator of SFTS in cats.

• Asian-Australasian Journal of Animal Sciences
• /
• v.24 no.7
• /
• pp.952-956
• /
• 2011

The objective of the study was to evaluate the effect of passive transfer status, determined by measuring serum immunoglobulin (Ig) concentration 24 hours after parturition, on growth performance in buffalo calves allowed to nurse the dam during the first month of life. Serum Ig concentration 24 hours after birth ranged from 28.1 to 35.9 mg/ml, birth weight ranged from 29 to 41 kg, body weight 30 days after birth ranged from 48.5 to 62.9 kg. The Average Daily Gain (ADG) from birth to day 30 ranged from 448 to 1,089 g/d. Significant linear associations were detected between serum Ig concentration 24 hours after birth and day-30 weight (p< 0.05; $R^2$ = 0.31) and between serum Ig concentration 24 hours after birth and ADG from birth to day 30 (p<0.001; $R^2$ = 0.72). Results indicated that passive transfer status was a significant source of variation in growth performance when buffalo calves nursed the dam. Maximizing passive transfer of immunity by allowing calves to nurse the dam can increase growth performance during the first month of life.

• Korean Journal of Veterinary Service
• /
• v.33 no.3
• /
• pp.303-307
• /
• 2010

The aim of this study was to determine the effects of body weight, organ weight, hematological values and biochemical parameters by estrogen on the ovariectomized (OVX) rats. The animals were divided into 4 groups. Intact group (n=10) received no treatment and operation. Sham group (n=10) received only sham operation and no treatment. OVX group (n=10) received operation and no treatment. OVX+E2(n=10) received operation and estrogen. The body weight of each group increased, but that of OVX+E2 group decreased. There were very significant differences (P<0.001) of body weights between OVX+E2 group and the other groups. Also, the wight of important organs such as heart, liver, spleen and kidney was measured. The heart weight was significantly lower(P<0.05) in the OVX+E2 group than the Intact group. The liver weight of the OVX+E2 group was significantly different in comparison with those of the other groups. On the other hand, there were no significantly differences in the organ weight of spleen and kidney between the OVX+E2 group and the other remaining groups. The hematological values of WBC, RBC, MeV, MCH and MCHC were not significantly different among the groups. The serum total protein and albumin decreased significantly in the OVX+E2 group as compared to those in the intact group. But, there were no significant differences in Ca, IP and Mg among the groups. We conclude that estrogen was significantly decreased the body weight in the ovariectomized rats. Our findings suggest that estrogen may influence the process of lipid packaging and absorption in the ovariectomized rats.