• Journal of Plant Biotechnology
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• 제6권2호
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• pp.125-130
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• 2004

Callus and cell suspension cultures of Eurycoma longifolia Jack could be an alternative supply of 9-hydroxycanthin-6-one and 9-methoxycanthin-6-one. The callus tissues were initiated from leaves of different trees. The friable calli were used for the preparation of the cell suspension cultures of E. longifolia. The leaf explant of tree Eu-9 produced the most callus and also induced high cell biomass in the cell suspension culture, but it produced low quantity of 9-methoxycanthin- 6-one and 9-hydroxycanthin-6-one. The leaf explant from tree Eu-8 produced low quantity of callus and cell biomass, but produced the highest quantity of 9-methoxycanthin- 6-one and 9-hydroxycanthin-6-one. Optimum production of cell biomass was obtained on cell culture medium with pH 5.75 prior to autoclaving, but high alkaloids content could be induced in culture medium in acidic condition with pH 4.75 and 5.25 prior to autoclaving.

• 한국식물병리학회지
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• 제13권2호
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• pp.132-137
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• 1997

Phytotoxicity of the culture filtrate and culture conditions for the production of the phytotoxin by Exserohilum monoceras 92-044 were examined. The necrotic lesions were developed on the leaves of Echinochloa crus-galli within 48 hrs after inoculation of the culture filtrate, and the leaves were completely blighted within 5∼7 days. Maximum toxicity was found in the culture broth containing 20% V-8 juice. Phytotoxin accumulation and fungal growth reached their highest peak at around 11 days. Typical symptom appeared on the leaf of E. crus-galli within 48 hrs. Only a weak chlorosis appeared on rice, Arundinella hirta (THUNB) and henry crabgrass (Digitaria sanguinalis SCOP.), but no further symptom developed.

• Fisheries and Aquatic Sciences
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• 제25권8호
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• pp.409-416
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• 2022

This study aims to know the effect of water-extracted horseradish Moringa oleifera leaf and KW21 fertilizer combination application on the density of Nannochloropsis sp. It was conducted in the Natural Food Laboratory of State Polytechnique, Tual. The experiment used complete randomized design with 5 treatments and 3 replications: A (25% horseradish leaf extract + 75% KW21), B (50% horseradish leaf extract + 50% KW21), C (75% horseradish leaf extract + 25% KW21), D (positive control of 100% KW21), and E (negative control of 100% horseradish leaf extract). Results showed that Treatment C yielded the best result, both the highest density of Nannochloropsis sp. and suitable harvest time.

• 한국초지조사료학회지
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• 제16권4호
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• pp.283-290
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• 1996

This experiment was carried out to determine the effects of soil condition that previous crop cultivated after [TO: Soil of corn cultivated, T1 : Soil of rye monoculture cultivated(l50kg/ha), T2 : Soil of mixedculture cultivated(Rye : Red clover = 120 : 3koa). T3 : Soil of mixed-culture cultivated(Rye : Red clover = 90 : 6kg/ ha), T4 : Soil of mixedculture cultivated(Rye : Red clover = 60 : 9kg/ha), T5 : Soil of mixedculture cultivated (Rye: Red clover = 30 : 12kg/ha), T6 : Soil of red clover monoculture cultivated(l5kgha)) on the growth characteristics and nutrient yield of silage corn. The results are summarized as follows. 1. The plant length of silage corn was high in order of T6>T5>T4 treatment. In the leaf length, T5 and T6 treatment showed short compared to the other treatments, but they were wider than the other treatments in leaf width. In the number of leaf, T6 treatment was the highest as 14 leaves per plant, while T1 treatment showed lower than the other treatments as 12.6 leaves per plant. 2. In the stem hardness, ear length, ear width and ear full degree, soil treatment of red clover mono-culture cultivation(T6) showed higher than the other treatment as 5.1 1kg/$cm^2$, 20.8~~1, 7.7cm and 97%. respectively (P<0.01, 0.05). Ihe leaf of summer depression did not many occurrence, but T6 treatment was lower than the other treatment as 0.8 leaf per plant. 3. ADF and NDF composition were not affected by soil condition. Crude protein composition of T6 treatment was the highest as 7.8%. while TI treatment was the lowest as 6.9%. Fresh weight yield(59,083kg/ha), dry matter yield(21,743kg/ha), crude protein yield(l,369kg/ha) and TDN yield(15,800kg/ha) at T6 treatment were much more increased by 41.9, 47.5, 57.4 and 49% than TI treatment(39,410, 14,259, 827 and 10,056kg/ha).

• 한국연초학회지
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• 제4권2호
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• pp.31-36
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• 1982

The field evaluation were conducted on dihaploid progenies derived from anther culture of F1 plant of Drama x Sohyang (N. tabacum L.) The mean values of agronomic and chemical traits of dihaploids such as Nicotine, length/width, plant height, Leaf length, leaves per plant and Yield were greater than those of tar parents. The correlation coefficient of dihaploids were similar to those of conventional lines and varieties. In path analysis, in relating yield components to yield, leaves per plant and leaf width had a direct effect on Yield, but the other components such as plant height, leaf length and days to flowering influenced Yield indirect way.

• 한국약용작물학회지
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• 제15권1호
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• pp.62-66
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• 2007

한국의 전통적인 민간 약재로 사용되어져온 흰민들레의 잎절편체로 부터 기관형성을 통한 재분화를 시도하였다. 잎 절편체를 BA (0-4mg/L)와 2,4-D (0-1mg/L)가 혼합 첨가된 MS 기본 배지에 치상하여 배양한 결과, 배양 3주후부터 신초가 발생되었다. 신초 분화율은 2mg/L BA 첨가된 조건에서 가장 높았으며, 배양 4주째 4mg/L BA 첨가된 조건에서는 캘러스가 유기되었다. 잎령에 따른 부정아 형성율, 발생빈도, 건량, 생중량을 조사한 결과, 발아 후 7주된 잎 절편체에서 평균 11.5개의 신초를 형성하였다. 유도된 신초는 0.5mg/L NAA가 첨가된 배지에서 발근되었으며, 화분에 옮겨 완전한 식물체로 재분화 되었다.

• 한국유기농업학회지
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• 제18권4호
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• pp.587-598
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• 2010

몇 가지 멀칭 재료가 감자의 생육과 잡초 방제에 미치는 영향을 구명코자 충북대학교 부속농장에서 2008년 8월 9일부터 2009년 6월 28일까지 수행하였다. 시험 품종은 '수미'였으며, 솔잎, 참나무잎, 짚, 왕겨, 톱밥 멀칭과 대조구(무멀칭) 등 6처리를 하였다. 재배시기에 따라 가을재배와 봄재배로 구분하였다. 본 연구 결과를 요약하면 다음과 같다. 가을감자의 초장, 경장, 엽장, 엽중, 괴경중은 솔잎 멀칭구에서 다른 처리와 유의성을 나타냈다. 가을감자 재배시 우점 잡초는 돌피, 명아주이었으며, 봄감자 재배시 우점 잡초는 돌피, 방동사니이었다. 멀칭에 따른 잡초 발생 억제 효과는 모든 멀칭구가 대조구와 유의성을 나타냈다. 수분함량은 왕겨 멀칭구에서 가장 높아 대조구와 차이를 나타냈다. 멀칭 처리구의 지온은 대조구에 비해 가을감자 재배시 $1.0{\sim}2.8^{\circ}C$ 낮았고, 봄감자 재배시 $0.7{\sim}2.3^{\circ}C$ 낮았다. 멀칭재료에 따른 지온은 솔잎 멀칭구, 짚 멀칭구, 낙엽 멀칭구, 왕겨 멀칭구, 톱밥 멀칭구, 대조구 순으로 낮았다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2003년도 제10차 국제학술회의 및 추계정기 학술발표회
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• pp.20-20
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• 2003

Isolation and culture of leaf protoplasts from two chilli cultivars (Capsicum annuum var. accumnatum and Bird chilli) were developed to enhance selection process in the somatic hybridization programmes. In order to isolate the protoplasts from leaves of these two chilli cultivars different incubation periods (3, 5 and 10 hours) were tested with combinations of enzyme mixtures containing cellulase and macerozyme. Leaves were incubated on three enzyme mixtures (2% cellulase +0.4% macerozyme, 1% cellulase +0.2% macerozyme and 0.5% cellulase +0.1% macerozyme in 13% mannitol) at 251oC in the dark. Three hours of incubation using 2% cellulase and 0.4% macerozyme was the best for the protoplast isolation of both chilli cultivars tested. The yield was 5${\times}$108protoplasts/m1/g leaf tissue in both chilli varieties. It was found that in the mixed nurse method using Nagata and Takebe (NT) medium supplemented with 1.0mg/12,4-D, NAA and BAP with 0.5M mannitol and 1.2% Sea Plaque agarose is the best medium for protoplast culture. Protoplasts of Capsicum annum var. accumnatum were alive for 14 days forming cell walls and initiating cell division.

• 한국작물학회지
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• 제37권5호
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• pp.419-424
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• 1992

콩에서의 조직배양 가능성을 알아보고자 콩 유묘를 기내에서 배양시킨 뒤, 여러 부위를 채취하여 각기 다른 배지 치상한 결과는 다음과 같다. 1. 줄기정단조직 배양에서는 대부분 shoot가 분화되어 완전한 식물체를 얻을 수 있었다. 2. 자엽절 조직배양에서는 multiple shoots가 나타나 대량증식에 유용했으나 배지에 따른 영향이 컸다. 3. 배축 및 상배축 조직배양에서는 callus만 분화되엇다. 4. 엽조직배양에서는 callus 분화 후 계대배양했을 때 shoot가 분화되었으나 완전한 식물체를 얻기는 힘들었다.

• 한국작물학회지
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• 제49권3호
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• pp.184-187
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• 2004

잎들깨 반촉성 재배시 흑색유공 polyethylene 멀칭효과와 파종적기 구명 및 입모향상 시험을 수행한 결과를 요약하면 다음과 같다. 1. 흑색유공 polyethylene 멀칭재배가 무멀칭재배에 비해 지온이 높고, 출현이 빠른 반면 성숙이 늦고, 경장이 크며, 엽수량뿐만 아니라 종실수량도 많았다. 2. 생육과 저온피해 등을 고려해 볼 때, 잎들깨의 안정적인 파종기는 1월 상순이었다. 3. 입모향상에 도움이 되는 피복재료는 부직포와 투명 polyethylene이나 출현 후 안정성이나 관리의 편의성 등을 고려해 볼 때 부직포가 적당하였다.