• The Plant Pathology Journal
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• v.34 no.3
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• pp.157-162
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• 2018

The objective of this work was to accomplish the isolation, molecular identification and characterizing the physiology of the causal agent of the algal spot in mango trees. For this purpose, the pathogen growth was assessed in different culture media, with subsequent observation and measurements of the filamentous cells. The molecular identification was made using mycelium obtained from leaf lesions and pure algae colonies grown in culture medium. Descriptions based on DNA sequencing indicated that the algae is Cephaleuros virescens. The algae must be isolated primarily in liquid medium for further pricking into agar medium. The highest mycelial growth average in Petri dishes occurred when the algae were grown in Trebouxia and BBM. Trebouxia enabled larger cells in the filamentous cells when compared to other culture media.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.29 no.4
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• pp.328-333
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• 1984

Variation in ploidy level of regenerated plants from rice anthers and effective diploidization methods of haploid plants were studied to obtain basic information in rice breeding through anther culture. In a total of 574 plants derived from anther culture using 14F$_1$ hybrids as materials, there were 49.7% haploids, 48.6% diploids and 1.7% polyploids, respectively. The frequency of haploids in Japonica/Indica crosses was 60.6%, and that of Japonica/Japonica crosses was 43.0% in average. Inclusion of 2.4-D or NAA as phytohormone may increase the frequency of haploids, but kinetin may increase the frequency of diploids. The rate of auto-diploidization by tiller separation of haploid plants showed 8.2% in average. The rate of diploidization by leaf-sheath injection of colchicine showed 18.8% in average. Morphological characters of haploids plants showed that 64.6% in culm length, 63.4% in panicle length, 68% in flag leaf length, and 74.4% in flag leaf width compared to diploid plants. These apparent morphological differences will contribute to identify the ploidy of plants derived from rice anther culture.

• Plant Biotechnology Reports
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• v.4 no.4
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• pp.321-328
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• 2010

An efficient protocol for in vitro organogenesis was achieved from callus-derived immature and mature leaf explants of Momordica charantia, a very important vegetable and medicinal plant. Calluses were induced from immature leaf explants excised from in vitro (15-day-old seedlings) mature leaf explants of vivo plants (45 days old). The explants were grown on Murashige and Skoog (MS) medium with Gamborg (B5) vitamins containing 30 g $1^{-1}$ sucrose, 2.2 g $1^{-1}$ Gelrite, and 7.7 lM naphthalene acetic acid (NAA) with 2.2 ${\mu}M$ thidiazuron (TDZ). Regeneration of adventitious shoots from callus (30-40 shoots per explant) was achieved on MS medium containing 5.5 ${\mu}M$ TDZ, 2.2 ${\mu}M$ NAA, and 3.3 ${\mu}M$ silver nitrate ($AgNO_3$). The shoots (1.0 cm length) were excised from callus and elongated in MS medium fortified with 3.5 ${\mu}M$ gibberellic acid ($GA_3$). The elongated shoots were rooted in MS medium supplemented with 4.0 ${\mu}M$ indole 3-butyric acid (IBA). Rooted plants were acclimatized in the greenhouse and subsequently established in soil with a survival rate of 90%. This protocol yielded an average of 40 plants per leaf explant with a culture period of 98 days.

• Korean Journal of Environmental Agriculture
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• v.42 no.4
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• pp.279-285
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• 2023

The demand for the fresh leaf of hooker chive, which is mainly used as functional roots and contains dietary sulfur or saponin, is increasing, but the leaves are only harvested 3-4 times per year under conventional field conditions. A plant factory system with different light qualities or intensities was applied for year-round production of the fresh leaves. Hooker chive (Allium hookeri) roots were hydroponically cultured under the plant factory with a mixture of blue plus red LEDs (Light-Emitting Diodes) and fluorescent lights for 50 weeks. Maximum leaf growth was attained with the 1.5 dS/m EC in the culture medium under the plant factory. The average leaf and shoot numbers of hooker chive grown hydroponically under a mixture of 200 µmol/m²/s LEDs increased by 147% and 140%, respectively compared to those under 100 µmol/m²/s LEDs at the 10th harvest. The leaf length of hooker chive grown under the LEDs treatment with the lowest light intensity significantly increased by 27% compared with the natural light treatment at the 10th harvest. However, there was no significant difference in leaf pigmentation between natural and 200 µmol/m²/s LEDs treatments. Plant factory with the mixture LEDs of blue and red lights can be applied for year-round production of hooker chive fresh leaves to ensure a stable supply of leafy vegetable throughout the year.

• Journal of Biosystems Engineering
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• v.32 no.2 s.121
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• pp.109-114
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• 2007

In mass production of seed-potato plantlets, the processes for in vitro propagation and ex vitro acclimatization with a high cost should be improved by a culture system with environmental control using scaled-up culture vessels. The experiment was conducted to design a hydroponic culture system for enhancement of growth and development of seed-potato (Solanum tuberosum) plantlets cultured under photoautotrophic (without sugar in culture medium) conditions with controlled light intensity and ventilation rate. The culture system was consisted of scaled-up culture vessels, ventilation pipes, a multi-cell tray and an environmental control system (ECS) for optimum controlling in temperature, light intensity, ventilation rate, and culture-medium supply. Growth and development of the plantlets was significantly increased under the ECS compared with a conventional culture system (CCS) of photomixotrophic culture (with sugar in culture medium) using small scale vessels. For 21 days, leaf area of the plantlets was expanded more than 2 times, and number of internodes also approximately 4 times greate. under the ECS. In addition, the photoautotrophic growth in sweetpotato (Ipomoea batatas) and chrysanthemum (Chrysanthemum morifolium) plantlets was greater more than 2 times compared with the CCS.

• Journal of Plant Biotechnology
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• v.30 no.4
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• pp.365-368
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• 2003

Variations of agronomic traits were evaluated on the progenies of regenerated rice plants of different callus origin and subculture period. From eighty-eight percent to ninety percents of ovary culture-derived plants (OCP) and anther culture-derived plants (ACP) were not segregated within the lines in major agronomic characters. Compare to ACP and OCP lines, the seed culture derived lines (SCP) showed more segregation (17％) in major agronomic traits among the lines. The most frequent segregating traits were grain fertility (6.1％) in ACP, leaf color (4.2％) in OCP and grain fertility (4.9％) and leaf color (4.9％) in SCP lines. The SCP line showed more variation in culm length, panicle length and heading date than those of OCP and ACP lines. The variation of agronomic traits in SCP lines was tended to increase with prolonged subculture. Culm and panicle length were shorter than those of original cultivar in all three types of tissue culture-derived lines (OCP, ACP and SCP).

• Fashion & Textile Research Journal
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• v.24 no.1
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• pp.95-107
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• 2022

In reality, the distinction between the Korean traditional culture and the cultures of other countries is at a delicate boundary. Additionally in the wake of the recent socio-cultural confrontation between Korea and China, it has become necessary to establish the foundation and area of Korean traditional culture and to actively utilize the importance of improving awareness of Korean traditional culture. To reorganize the reckless use of the gold foil pattern shown in the rental hanbok, data on the museum's collection of gold leaf patterns were collected and analyzed. Based on the gold foil, Gilsang characters such as Phoenix pattern, Crane pattern, Bat pattern, Flower pattern, Fruit pattern and recovery advice were extracted through references. The traditional gold foil pattern was reconstructed and relocated to design the gold leaf patch. Based on the collection and analysis of the museum's relics, the Wonsam & Daedae, Dangeui, Sranchima, Sagyusam, Jeonbok, Bokgun, and Daenggi were produced. Therefore, we present the possibility of producing gold foil and modern methods for producing gold foil using laser cutting techniques that can express refinement and complexity well, and gold foil thermal transfer paper with retouchable effects. Additionally, we would like to reflect upon the practicality and the convenience to modern people by considering the complexity and hassle of the traditional gold foil production process, and the disadvantages of processes that require relatively longer time. It intends to help revitalize the market of Korean traditional clothing and fashion products.

• Proceedings of the Korean Society of Crop Science Conference
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• 2005.08a
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• pp.100-113
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• 2005

Leaf color and plant vigor are key indicators of crop health. These visual plant attributes are frequently used by greenhouse managers, producers, and consultants to make water, nutrient, and disease management decisions. Remote sensing techniques can quickly quantify soil and plant attributes, but it requires humans to translate such data into meaningful information. Over time, scientists have used reflectance data from individual wavebands to develop a series of indices that attempt to quantify things like soil organic matter content, leaf chlorophyll concentration, leaf area index, vegetative cover, amount of living biomass, and grain yield. The recent introduction of active sensors that function independent of natural light has greatly expanded the capabilities of scientists and managers to obtain useful information. Characteristics and limitations of active sensors need to be understood to optimize their use for making improved management decisions. Pot experiments involving sand culture were conducted in 2003 and 2004 in a green house to evaluate corn and red pepper biomass. The rNDVI, gNDVI and aNDVI by ground-based remote sensors were used for evaluation of corn and red pepper biomass. The result obtained from the case study was shown that ground remote sensing as a non-destructive real-time assessment of plant nitrogen status was thought to be a useful tool for in season crop nitrogen management providing both spatial and temporal information.

• Journal of Fashion Business
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• v.19 no.1
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• pp.95-105
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• 2015

This study investigated the color culture in the Japanese Medieval Age. The Japanese Medieval Age included the Kamakura period (1180-1333) and Muromachi period (1336-1573), and the leading group transitioned from the Kuge families to the Buke families. The taboos about colors from ancient times became nominal, and forbidden colors, such as purple, celadon, and red, became the colors of the samurai, leading to beautiful soldier gears that were unparalleled in history. In the Kamakura period, colors that conveyed a strong impression were created and preferred with the combination of a samurai's reasonable spirit and zen thoughts. The period was also called "the era of hari", and cross dyeing based on basic colors such as suou (red), ai (blue), and kuchinasi (yellow) was popular. In both the Kamakura and Muromachi periods, conspicuous and strong colors were sought for costumes, and embroidery was used with gold leaf, silver leaf, gold threads, silver threads, and background color. The colors of costume preferred by Buke men in the period included green, blue, and brown. In the characteristics of the kosode, the sugan and hitadare were used for men's formal dress, while kosode was used for the grooming of the working class. In these periods, additionally, the working class began to be socially engaged in actively wearing the one-layer kosode, which became popular, and the characteristics of the Japanese Medieval Age, during which functionality and practicality was valued, were also reflected in the dressing.

• Journal of Korean Society of Forest Science
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• v.80 no.1
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• pp.1-8
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• 1991

Developmental micropropagation method and somatic embryogenesis for hybrid poplars, Populns ehrarnericana Eco28, P. nigra ${\times}$ P. moximowiczii 62-9, were established using nodule culture system. Calli of Eco28 and 62-9 clone were initiated from leaf explant on the medium with 0.5mg/l and 2.0mg/l 2, 4-D, respectively. Cell suspension culture was established from callus derived from leaf explant culture. When suspended on MS medium with optimal combination of BA and NAA fine nodules were obtained after 2 weeks of culture. For shoot regeneration, nodules were transferred into liquid and agar solidified medium. Numerous shoots were regenerated from nodules of 62-9 on liquid media. Organogenesis was effectively achieved on agar solidified regeneration media containing different concentrations of BA and adenine sulfate. Average numbers of 27 and 24 shoots per nodule were induced from 62-1 and Eco28 clones after 8 weeks of culture, respectively. In addition, somatic embryogenesis also occurred in the same regeneration medium. This procedure can be applied to vegetative propagation, utilization of somaclonal variation, production of secondary metabolite and materials of biotechnology research.