Plant Biotechnology Reports

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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High-frequency shoot regeneration from leaf explants through organogenesis in bitter melon (Momordica charantia L.)

  • Thiruvengadam, Muthu (Graduate Institute of Biotechnology, National Chung-Hsing University) ;
  • Rekha, K.T. (Graduate Institute of Biotechnology, National Chung-Hsing University) ;
  • Yang, Chang-Hsien (Graduate Institute of Biotechnology, National Chung-Hsing University) ;
  • Jayabalan, Narayanasamypillai (Department of Plant Biotechnology, Bharathidasan University) ;
  • Chung, Ill-Min (Department of Applied Life Science, Konkuk University)
  • Received : 2009.12.17
  • Accepted : 2010.09.06
  • Published : 2010.12.30
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Abstract

An efficient protocol for in vitro organogenesis was achieved from callus-derived immature and mature leaf explants of Momordica charantia, a very important vegetable and medicinal plant. Calluses were induced from immature leaf explants excised from in vitro (15-day-old seedlings) mature leaf explants of vivo plants (45 days old). The explants were grown on Murashige and Skoog (MS) medium with Gamborg (B5) vitamins containing 30 g $1^{-1}$ sucrose, 2.2 g $1^{-1}$ Gelrite, and 7.7 lM naphthalene acetic acid (NAA) with 2.2 ${\mu}M$ thidiazuron (TDZ). Regeneration of adventitious shoots from callus (30-40 shoots per explant) was achieved on MS medium containing 5.5 ${\mu}M$ TDZ, 2.2 ${\mu}M$ NAA, and 3.3 ${\mu}M$ silver nitrate ($AgNO_3$). The shoots (1.0 cm length) were excised from callus and elongated in MS medium fortified with 3.5 ${\mu}M$ gibberellic acid ($GA_3$). The elongated shoots were rooted in MS medium supplemented with 4.0 ${\mu}M$ indole 3-butyric acid (IBA). Rooted plants were acclimatized in the greenhouse and subsequently established in soil with a survival rate of 90%. This protocol yielded an average of 40 plants per leaf explant with a culture period of 98 days.

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References

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