• The Korean Journal of Mycology
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• v.37 no.2
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• pp.167-172
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• 2009

Mycelia of Pleurotus ostreatus, Phellinus linteus, Ganoderma lucidum and Lentinus edodes were cultured in the selected cereals to generate functionally active cereals. The optimum water contents for the mycelial growth were 50%(wt/wt) for brown rice, barley and soybean and 75% for wheat and corn, respectively. P. ostreatus grew well in the most cereals while the mycelial growth of P. linteus, G. lucidum and L. edodes in soybean were siginificantly retarded. The contents of β-glucan and glucosamine in the mycelial cereals were determined. Wheat cultured with mushroom mycelia showed high ß-glucan content. Especially, wheat with G. lucidum contained the highest value of 26.16%. Soybean cultured with G. lucidum showed two-fold increase in glucosamine content with 9.63% of total mass while wheat showed 7.91%. Overall, wheat cultured with G. lucidum was the best functional cereal in terms of β-glucan and glucosamine contents.

• Journal of Microbiology and Biotechnology
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• v.19 no.6
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• pp.634-634
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• 2009

• 한국생물공학회:학술대회논문집
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• 2005.04a
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• pp.502-505
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• 2005

This study was concentrated to investigate the dependency of recovery yield on the extraction conditions. From fruiting body, the extracted amount of polysaccharide was studied with various solvent. In order to maximize the yield, the optimum extraction conditions were elucidated with respect to solvent, temperature and time. In addition, the dialysis was applied to obtain the higher purity of polysaccharide with varying the exposure time.

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 1998.10a
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• pp.63-65
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• 1998

최근, 생표고의 수요가 급증하고 있으며 주년적인 생산과 공급을 원활하게 하기 위해서 시설재배가 필수적으로 요구되고 있다. 1996년도에 있어 표고 골목은 8,433천㎥이었는데, 이 가운데 노지재배가 92%, 시설재배가 8%를 각각 차지하고 있으나 생산량에서는 노지재배가 90% 그리고 시설재배가 10%를 차지하고 있다. 또한 표고의 시설재배를 통하여 생산량은 약 20%, 생산액은 약 50% 각각 증가하여 소득 면에서도 기여하고 있다. (중략)

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 1994.05a
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• pp.98-101
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• 1994

표고는 다른 농산물에 비하여 품질등급을 결정하는 외관 특징들이 갓의 전후면에 걸쳐 복잡하게 분포하고 있어 자동 선별장치를 구현하기 위해서는 갓의 전후면 인식과 아울러 외관특징을 검출해야 한다. 특히 건조 표고의 경우 갓 바깥으로 튀어나온 꼭지부의 존재 여부 및 상태가 갓의 크기, 모양 및 후면 내피의 말린 정도를 검색하는 데 큰 영향을 미친다. (중략)

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 1999.11a
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• pp.221-224
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• 1999

표고(Lentinus edodes (Berk.) Sing.)는 송이과 Tricholomataceae에 속하는 식용 버섯으로서, 동북아시아 지역에서 가장 인기 있는 재배 버섯이다. 표고의 수요와 생산량을 매년 꾸준히 증가하고 있고, 1985년 표고 생산량은 880톤, 87년 1,080톤, 89년 1,530톤, 91년 1,761톤, 92년의 표고의 재배면적은 397,340평이고, 생산량은 2,253톤이며 금액으로 375억에 이르고 있다. (중략)

• Journal of Biosystems Engineering
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• v.19 no.2
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• pp.124-137
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• 1994

Visual features of a mushroom(Lentinus Edodes, L.) are critical in grading and sorting as most agricultural products are. Because of its complex and various visual features, grading and sorting of mushrooms have been done manually by the human expert. To realize the automatic handling and grading of mushrooms in real time, the computer vision system should be utilized and the efficient and robust processing of the camera captured visual information be provided. Since visual features of a mushroom are distributed over the front and back sides, recognizing sides and states of the stalk including the stalk orientation from the captured image is a prime process in the automatic task processing. In this paper, the efficient and robust recognition process identifying the front and back side and the state of the stalk was developed and its performance was compared with other recognition trials. First, recognition was tried based on the rule set up with some experimental heuristics using the quantitative features such as geometry and texture extracted from the segmented mushroom image. And the neural net based learning recognition was done without extracting quantitative features. For network inputs the segmented binary image obtained from the combined type automatic thresholding was tested first. And then the gray valued raw camera image was directly utilized. The state of the stalk seriously affects the measured size of the mushroom cap. When its effect is serious, the stalk should be excluded in mushroom cap sizing. In this paper, the stalk removal process followed by the boundary regeneration of the cap image was also presented. The neural net based gray valued raw image processing showed the successful results for our recognition task. The developed technology through this research may open the new way of the quality inspection and sorting especially for the agricultural products whose visual features are fuzzy and not uniquely defined.

• Journal of Nutrition and Health
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• v.28 no.11
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• pp.1091-1099
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• 1995

The cyutotoxic effects of hot water soluble polysaccharides extract(PS) from the mushroom, Lentinus edodes, and in combinations with vitamin A or vitamin E on life span of ICR mice bearing P388 cancer cells and in vitro against P388 cancer cells were examined. The chemical components of PS and fractions were analyzed and survival time and cell number of P388 treated with extract fractions with and without vatamin A or E supplementation were also measured. The results obtained were summarized as follows; The extract of fraction B was shown to have the highest antitumor activity against P388 implanted in ICR mice. The antitumor fraction B was consisted of 82.0% of polysaccharide and 4.2% of protein. All three fractions seemed to have in vivo antitumor activity against P388, and fraction B showed the highest activity, In vitro P388 cell growht was inhibited 76%, 89%, 54% by the addition of fraction A, B and C respectively. Vitamin A or E did not appear to have any accelerating effects on either in vivo or in vitro cell cytotoxicity when each of them was combined with the PS and fractions. All three fractions contained more than 68% of polysaccharides. The fraction B showed the highest value of 88% in polysaccharides. Monsaccharides of the fraction B were identified as galactose(59.1%), glucose(29.2%), fructose(2.8%) and uronic acid(4.2%). Hydrolysis of protein from the fraction B was didentifed to have 17 kinds of defined and 5 undifined amino acids. The inhibitory effects of the hot water extracts from mushroom against cancer cell growth of P388 were stronger than the control group. And the survival time of ICR mice was shown to be 161% between the control group and the experimental groups.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 1993.10a
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• pp.1243-1253
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• 1993

Visual features of a mushroom(Lentinus Edodes L) are critical in sorting and grading as most agricultural products are. Because of its complex and various visual features, grading and sorting of mushrooms have been done manually by the human expert. Though actions involved in human grading looks simple, a decision making undereath the simple action comes form the results of the complex neural processing of the visual image. And processing details involved in the visual recognition of the human brain has not been fully investigated yet. Recently, however, an artificial neural network has drawn a great attention because of its functional capability as a partial substitute of the human brain. Since most agricultural products are not uniquely defined in its physical properties and do not have a well defined job structure, a research of the neuro-net based human like information processing toward the agricultural product and processing are widely open and promising. In this pape , neuro-net based grading and sorting system was developed for a mushroom . A computer vision system was utilized for extracting and quantifying the qualitative visual features of sampled mushrooms. The extracted visual features and their corresponding grades were used as input/output pairs for training the neural network and the trained results of the network were presented . The computer vision system used is composed of the IBM PC compatible 386DX, ITEX PFG frame grabber, B/W CCD camera , VGA color graphic monitor , and image output RGB monitor.

• Journal of Applied Biological Chemistry
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• v.61 no.1
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• pp.69-73
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• 2018

Oak mushroom (Lentinus edodes) is cultivated by using oak logs and sawdust medium. Green mold (Trichoderma) infection on these media severely suppresses the growth of oak mushroom. Usages of antibiotics and chemicals are not generally allowed to control of green mold since the mushroom is a fresh food. Tolaasin and its analogues, peptide toxins secreted by Pseudomonas tolaasii, have the antifungal activity and they have been successful to control the green mold disease. When the green mold, Trichoderma harzianum H1, was cultured in the presence of these toxins, the growth of fungus was effectively suppressed. In sawdust media, when the bacterial culture supernatants were sprayed on the aerial hyphae of green molds, the fungal growth was completely suppressed. Particularly, the antifungal activity was greatly increased by the combined culture extracts of P. tolaasii 6264 and HK11 strains. Therefore, these bacterial strains and their peptide toxins were able to suppress the growth of green molds and these can be good candidates to prevent from Trichoderma disease in oak mushroom cultivation.