Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Increase in antifungal activity by the combination of tolaasin and its analogue peptides

톨라신류 펩티드 혼합처리에 의한 항진균 활성의 증가

  • Yun, Yeong-Bae (Department of Environmental and Biological Chemistry, Chungbuk National University) ;
  • Lee, Hyoung-Jin (Department of Environmental and Biological Chemistry, Chungbuk National University) ;
  • Kim, Young-Kee (Department of Environmental and Biological Chemistry, Chungbuk National University)
  • Received : 2018.02.12
  • Accepted : 2018.02.22
  • Published : 2018.03.31
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Abstract

Oak mushroom (Lentinus edodes) is cultivated by using oak logs and sawdust medium. Green mold (Trichoderma) infection on these media severely suppresses the growth of oak mushroom. Usages of antibiotics and chemicals are not generally allowed to control of green mold since the mushroom is a fresh food. Tolaasin and its analogues, peptide toxins secreted by Pseudomonas tolaasii, have the antifungal activity and they have been successful to control the green mold disease. When the green mold, Trichoderma harzianum H1, was cultured in the presence of these toxins, the growth of fungus was effectively suppressed. In sawdust media, when the bacterial culture supernatants were sprayed on the aerial hyphae of green molds, the fungal growth was completely suppressed. Particularly, the antifungal activity was greatly increased by the combined culture extracts of P. tolaasii 6264 and HK11 strains. Therefore, these bacterial strains and their peptide toxins were able to suppress the growth of green molds and these can be good candidates to prevent from Trichoderma disease in oak mushroom cultivation.

표고버섯(Lentinus edodes)은 원목이나 톱밥배지를 사용하여 재배한다. 이 배지에 푸른곰팡이(Trichoderma)가 감염되면 표고버섯의 생장을 크게 억제한다. 버섯은 신선식품이기 때문에 푸른곰팡이를 방제하기 위한 항생제와 화학약품의 사용은 허용되지 않는다. Pseudomonas tolaasii에 의해 분비되는 펩티드 독소인 톨라신과 톨라신 유사체들은 항진균 활성을 가져 푸른곰팡이병 방제에 성공적이었다. 푸른곰팡이 Trichoderma harzianum H1에 톨라신 펩티드를 처리하였을 때 곰팡이의 성장은 효과적으로 억제되었고, 실제 톱밥배지에서 균주 배양액을 푸른곰팡이 균사에 분사하였을 때, 균사 성장은 완벽하게 억제되었다. 특히, P. tolaasii 6264와 HK11 균주의 배양 추출액을 혼합하여 처리하였을 때에 항진균 활성이 크게 증가하였다. 따라서 이러한 균주들과 펩티드 독소들은 푸른곰팡이의 성장을 억제할 수 있고, 표고버섯 재배에서 푸른곰팡이병을 방제하기 위한 좋은 후보가 될 수 있다.

Keywords

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