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Different Combinations of Condensed Chlorella and Baker′s Yeast for Mass Culture of the Freshwater Rotifer, Brachionus calyciflorus Pallas (담수산 농축 Chlorella와 빵효모의 혼합 공급 비율에 따른 담수산 Rotifer, Brachionus calyciflorus Pallas의 대량배양)

  • 이균우;박흠기;박기명
    • Journal of Aquaculture
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    • v.13 no.2
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    • pp.147-152
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    • 2000
  • To reduce the production cost of the rotifer, different combinations of Chlorella and Baker's yeast were used to mass culture of Brachionus calyciflorus. Rotifer density tended to decrease abruptly at 100% Chlorella-feeding, when unionized ammonia level ranged from 14.1 to 29.6 ppm and DO level from 0.2 to 0.3 ppm. Hence DO level > 0.3 ppm and unionized ammonia level < 14 ppm should be maintained. Production cost of the rotifer was the highest, when a combination of 100 % Chlorella was chosen as food source, but it was about 1.8 times less, when a combination of 70 % Chlorella and 30% yeast was used as food source. However, the highest rotifer density remained almost at the same level, when either of the combination was chosen as food source. A combination of 70 % Chlorella and 30 % yeast is recommended for maximum production of the rotifer at the lowest feed cost.

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Effects of Systematic Variation Application of Fe, Mn, Cu, and Zn on the Dry Matter Yields of Orcharograss and White Clover (Fe, Mn, Cu 및 Zn의 Systematic Variation 시비가 Orchardgrass 및 White Clover의 건물수량에 미치는 영향)

  • Jung, Yeun-Kyu
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.24 no.3
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    • pp.193-200
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    • 2004
  • This pot experiment was conducted to investigate the effects of systematic variation application of Fe, Mn, Cu, and Zn on forage performance of orchardgrass and white clover. The treatments of systematic variation were 0/100, 25/75, 50/50, 75/25, and $100/0\%$ in the Fe/Cu(trial-1), Mn/Zn(trial-2), and Fe+Cu/Mn+Zn(trial-3), respectively. The treatments of Fe/Mn/Cu/Zn(trial-4) were composed of $70\%$ in main element and $10\%$ in other 3 elements, respectively. 1. By the systematic variations of Fe, Mn, Cu, and Zn, the yields were more significantly influenced in white clover than in orchardgrass. In addition, the yields of white clover were closely correlated to the trends of root/nodule growth and flowering. In the Fe/Cu trial, the relatively high yields were obtained at the $100/0\%$ in orchardgrass and at the $75/25\%$ in white clover. The yields of white clover were more negatively influenced by the 100/0(Cu control) than by the 0/100(Fe control). The yields of orchardgrass, however, tended to be opposite to the above trends. 2. In the Mn/Zn trial, both forages showed generally high yields at the high ratios of Mn/Zn. Compared with orchardgrass, the yields of white clover were greatly decreased by the Mn-deficiency(low ratio of Mn/Zn). The effects of Zn on forage yields, however, were not recognized. 3. In the Fe+Cu/Mn+Zn trial, the yields of orchardgrass tended to be slightly different among the treatments. The yields of white clover, however, were relatively' high at the 75/25, and showed a severe decrease at the 100/0 in the 2nd half cuts. In the Fe/Mn/Cu/Zn trial, the yields of white clover tended to be relatively high at the Cu and Zn treatments. It was likely to be caused by the balanced Fe/Mn ratio.

Influences of Polycyclic Aromatic Hydrocarbons on Soybean and Rice Growth (다환방향족탄화수소가 콩과 벼의 생육에 미치는 영향)

  • Kim, Young-Ju;Shim, Doo-Bo;Song, Sun-Hwa;Kim, Seok-Hyeon;Chung, Jong-Il;Kim, Min-Chul;Chung, Jeong-Sung;Kim, Hyung-Gon;Shim, Sang-In
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.59 no.2
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    • pp.181-187
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    • 2014
  • Polycyclic aromatic hydrocarbons (PAHs) are a group of ubiquitous hazardous pollutants derived from fossil fuel, various combustion sources and pyrolysis of a wide range of plastics. Because PAHs can be uptake into crop plants, the inhibitory effects on rice and soybean plants were examined in greenhouse and growth chamber experiment. Soil-applied PAHs (phenanthrene of 0, 10, 30, 100 ppm) slightly reduced the plant height and dry weight both in transplanted rice and soybean plant. The inhibitory effect on growth was greater in soybean than rice. Plant height of soybean plants treated by 100 ppm was 58.9 cm and this value was 87.2% of untreated plant. In rice plant, the plant height was less inhibited (96.0% of untreated plant) by 100 ppm at 80 days after treatment (DAT). However, leaf chlorophyll content and chlorophyll fluorescence were less inhibited by PAHs at late growth stage (after heading) although the photosynthesis-related parameters were slightly inhibited from 20 DAT to 70 DAT. In agar medium experiment with infant seedlings, inhibition of seedling length and fresh weight by phenanthrene at 100 ppm were greater as compared to the experiment with adult plant in pot. Seedling length and fresh weight were reduced by 54.2% and 33.3% for rice and 27.9% and 13.2% for soybean, respectively. The results reflected that PAHs were more inhibitory during juvenile stage than adult stage and more inhibitory to rice plant than soybean for juvenile stage.

Primary Extranodal Non-Hodgkin's Lymphoma: Clinicopathological Features, Survival and Treatment Outcome in Two Cancer Centers of Southern Turkey

  • Mertsoylu, Huseyin;Muallaoglu, Sadik;Besen, Ayberk Ali;Erdogdu, Suleyman;Sezer, Ahmet;Sedef, Ali Murat;Kose, Fatih;Arican, Ali;Ozyilkan, Ozgur
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.17
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    • pp.7207-7211
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    • 2014
  • Background: The aim of this study was to assess the epidemiological and clinicopathological characteristics of primary extranodal non-Hodgkin's lymphoma (pENL) patients, focusing on treatment and survival outcome. Materials and Methods: Between October 2003 and March 2012, 802 patients with non-Hodgkin's lymphoma (NHL) were diagnosed and treated in two different cancer centers of Southern Turkey. Results: pENL, constituted 12.4% (100/802) of all NHL studied during this period. Median age of the patients was 56 years (range 17-87 years) and the male: female distribution was 3:2. Eighty-five of 100 patients (85%) were in stage I/II, 9/100 (9%) in stage III, whereas 6/100 (6%) were in stage IV. Head and neck constituted the most common site (51/100, 51%), followed by gastrointestinal tract (GIL) (37/100, 37%), and cerebrum (CL) (5/100, 5%). Diffuse large B cell lymphoma (DLBCL) was the most common histological type, observed in 53% of patients, followed by marginal zone extranodal lymphoma (13%). Most of patients (76%) received a CHOP containing regimen. Complete remission (CR) were achieved in 71% of patients. The median follow-up duration of all patients was reported as 37.6 months (range, 0.8-165 months). This period was reported as 137.5 months (range, 117.5-1578.6 months) in gastrointestinal lymphoma (GIL) patients, 119.0 months (range, 91.8-146.1 months) in head and neck lymphoma (HNL) patients, and 18.4 months (range, 12.6-24.1 months) in cerebral lymphoma (CL) patients. Conclusions: Head and neck, and the gastrointestinal tract were the two most common extranodal sites observed. Histologically DLBC accounted for the majority of cases. Most patients were on earlier stages, had low-low intermediate IPI scores and had a favorable prognosis.

Studies on Nutritional Analysis and Antioxidant activity of Oriental Medicines with Bloodstream Improvement (혈류개선 효능을 지닌 한약재의 영양성분 분석 및 항산화 활성에 관한 연구)

  • Park, Sung-Hye;Park, Byung-Ju;Park, Hae-Ryoung
    • Journal of Digital Convergence
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    • v.14 no.10
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    • pp.563-570
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    • 2016
  • This study investigated applicable values and developments of 5 different kinds of oriental medicine materials as functional supplements or medicinal uses, known to improve blood circulation by means of analyzing nutritional compositions and antioxidant activities. Among general nutritional compositions, protein, iron and copper contents in Polygini Multiflori Radix (20.28%, $132.22{\mu}g$/100 g, $26.54{\mu}g$/100 g), Angelica Gigantis Radix (16.54%, $98.20{\mu}g$/100 g, $32.15{\mu}g$/100 g) and Paeoniae Radix(18.67%, $62.10{\mu}g$/100 g, $20.32{\mu}g$/100 g) were significantly higher than other materials. Angelica Gigantis Radix had the highest of total polyphenols and flavonoid contents, Polygini Multiflori Radix (middle), Paeoniae Radix (the lowest). Antioxidant activities by using DPPH radicals removal showed the highest in Polygini Multiflori Radix, Angelica Gigantis Radix (middle), Paeoniae Radix (the lowest). This study tried to use integrated approaches, including Western and Oriental medicine to improve blood circulation and to set up base to develop products originated from natural compositions.

Effect of Electric Conductivity and Potassium Level of the Culture Solution on the air Pollution Sensibility of Perillar Frutescens Suwon 16 (양액의 전기전도도 및 칼륨 수준이 들깨 수원 16호의 대기오염 지표성에 미치는 영향)

  • Kim, Jeong-Gyu;Lee, Yong-Bum;Koh, Kang-Suk
    • Korean Journal of Soil Science and Fertilizer
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    • v.29 no.4
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    • pp.411-418
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    • 1996
  • This experiment was conducted to investigate the effect of electric conductivity (EC) and potassium (K) concentration level in the culture solution on the growth and the air pollution monitoring capability of Perilla frutescens Suwon 16 which was recognized as a possible bioindicator for air pollution. The plants were exposed to $NO_2$, $O_3$, $SO_2$ and $NO_2+SO_2$ within a phytotron at $25^{\circ}C$ (day) or at $20^{\circ}C$(night) with 70% of relative humidity and evaluated the effect of EC and K level on the injury of the plants, The highest dry weights were gained as 16.3 g/plant at 0.5 dS/m of EC and as 32.3 g/plant at 100 and 200mg/l of K concentration, respectively. The diffusive resistances appeared as low values at 0.5 dS/m of EC and at 50, 100mg/l of K concentration. The increasing of the visible injury with increasing the dose of air pollutants could be taken at 0.5. 1.0 ds/m of EC and at 50, 100mg/l of K. The recommendable level of EC and K of the culture solution were 0.5-1.0 dS/m of electric conductivity. 50-100mg/l of K for the plant as an air pollutant biomonitor, when the 7th or 8th leaf was developed.

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Antimutagenic Effects of Browning Products Reacted with Polyphenol Oxidase Extracted from Apple (사과 Polyphenol Oxidase에 의한 효소갈변반응 생성물의 항돌연변이 효과)

  • Baik, Chang-Weon;Ham, Seung-Shi
    • Korean Journal of Food Science and Technology
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    • v.22 no.6
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    • pp.625-631
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    • 1990
  • This study was carried out to investigate the antimutagenic effects of five kinds of apple enzymatic browning reaction products(AEBRP) on mitomycin C (MMC), N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), 4-nitroquinoline-1-oxide(4NQO), benzo(${alpha}$)pyrene(B(${alpha}$)P) and 3-amino-1,4-dimethyl-5H-pyri-do [4,3-b]indol (Trp-P-1). In spore rec-assay using B. subtilis Hl7($rec^+$) and M45($rec^-$), homocatechol-AEBRP and hydroquinone-AEBRP showed strong antimutagenic effects on MMC and MNNG as the concentration of AEBRP increased. In the Ames test using S. typhimurium TA98 and TA100, hydroxyhydroquinone-AEBRP and pyrogallol-AEBRP showed strong antimutagenic effects on Trp-p-1 and B(${alpha}$)p in TA98 and TA100 in the presence of S-9 mix. Most of AEBRPs suppressed about 50% to 80% the mutagenesis in S. typhimurium TA98 induced by MNNG, however, AEBRPs except hydroxyhydroquinone-AEBRP showed antimutagenic effects of about 94% in TA100. Antimutagenic effects of the five kinds of AEBRPs on 4-NQO were more or less weak, in particular homocatechol-AEBRP exhibited the inhibitory effect of about 48% in TA98, and homocatechol-AEBRP and hydroquinone-AEBRP showed inhibitory effects of about 46% to 58% in TA 100.

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Iodine Supplementation of Leucaena leucocephala Diet for Goats. I. Effects on Nutrient Utilization

  • Rajendran, D.;Pattanaik, A.K.;Khan, S.A.;Bedi, S.P.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.6
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    • pp.785-790
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    • 2001
  • Twelve indigenous male goats, comprising of six intact and six castrated (2.5-3 years; $24.4{\pm}0.62kg$) were assigned evenly into two dietary treatments, viz. $I_0$ and $I_{100}$ and were used to study the effect of supplementation of iodine on the nutrient utilization when their diet contained Leucaena leaf meal. They were offered a conventional concentrate mixture along with Leucaena leucocephala leaf meal, the latter to meet 50% of their crude protein (CP) requirements, and supplemented with either no iodine ($I_0$) or 0.1 mg of iodine ($I_{100}$)/day/animal as potassium iodide for a period of 105 days. Wheat straw given ad libitum was the sole source of roughage. A metabolism trial of 8 days duration was conducted after 90 days of experimental feeding. It was observed that the overall dry matter (DM) intake during experimental period was higher (p<0.05) in $I_{100}$ group as compared to $I_0$ group (508.6 vs. $443.7g\;d^{-1}$). The intake of CP, digestible crude protein (DCP) and metabolisable energy (ME), although non-significant, tended to be higher in the iodine supplemented group, $I_{100}$. Digestibility of dry matter, organic matter (OM), CP, ether extract and crude fiber (CF) did not differ (p>0.05) between the treatments. However, nitrogen retention was higher (p<0.01) in $I_{100}$ than $I_0$ with the values being 2.63 and $1.70g\;d^{-1}$, respectively. No difference (p>0.05) was evident in the retention of calcium and phosphorus between the two groups. The castrated animal exhibited lower DM intake concurrent with higher digestibility of DM and crude fibre (p<0.05), and organic matter and total carbohydrates (p<0.01) when compared to intact ones. It was concluded that supplementation of iodine to leucaena based ration may help in improving the DM intake and nitrogen utilization by goats.

Antioxidant, Antibacterial and Anti-inflammatory Effects of Stachys sieboldii Extract (초석잠 추출물의 항산화, 항균 및 항염 활성)

  • Lee, Jeong Ho
    • Korean Journal of Plant Resources
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    • v.34 no.5
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    • pp.420-432
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    • 2021
  • This study was conducted to evaluate antioxidant, antibacterial, anti-inflammatory, and digestive enzyme activity in water extract (SAW) and 60% ethanol extract (SAE) from Stachys sieboldii. As the treatment concentration of each extract S. sieboldii extract increased, antibacterial and antioxidant activity increased. The total polyphenol and total flavonoid contents of SAW were 106.25 ± 0.94 mgGAE/g, 24.4 ± 0.24 mgQE/g and SAE were 124.61 ± 1.11 mgGAE/g, 45.2 ± 3.52 mgQE/g, respectively. The 400 ㎍/mL of SAW and SAE performed more than 53% protective effects against oxidative stress in HepG2 cell lines. All extracts were not showed cytotoxicity to RAW 264.7 cell line at 100 ㎍/mL. NO production was reduced to 44.3 ± 1.4% for SAW and 45.1 ± 1.0% for SAE at a concentration of 100 ㎍/mL. The production of inflammatory cytokines each TNF-α, IL-1β, and IL-6 was inhibited in a concentration-dependent. S. sieboldii extract did not showed Caco-2 cells cytotoxicity and inhibited NO production in concentration-dependent. As the concentration of the S. sieboldii extract increased of α-amylase and protease enzymes activity, which are digestive enzyme. As a result of the experiment, it is judged that it can be used as basic data for the development of health food using S. sieboldii.

Bacteriological Characteristics of Drinking Water in Pusan Area (부산지역 음용수의 세균학적 특성)

  • 김용관
    • Journal of Environmental Health Sciences
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    • v.19 no.2
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    • pp.34-39
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    • 1993
  • One hundred and eighty-seven water samples were collected from 23 of spring water, 2 of ground water, 1 of tap water in Pusan area and 3 of natural mineral waters. Total coliform group, fecal coliform, viable cell count and microflora were investigated to evaluate water quality of drinking water. The results were as follows: range and geometric mean value of total coliform and fecal coliform MPN's of spring water were 0~1,500/100 ml, 85/100 ml and 0~460/100 ml, 24/100 ml but coliform group was not detected in the samples of tap water and natural mineral water. Viable cell count of spring water, ground water and tap water were lower as 100 cell than the criteria for drinking water but that of natural mineral water was higher as 6.5X 10$^2$~7.4X 10$^3$ /ml. Predominant speces among the 219 strains isolated from the samples were 19.6% Aeromonas spp., 19.2% Enterobacteriaceae, 16% Acinetobacter spp. Especially, spring water and vessels were contaminated by Hafnia spp. and Providencia Spp, inhabitant of the oral cavity.

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