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Insecticidal effects of Anethum graveolens and Artemisia vulgaris oils against Sitophilus zeamais and Sitotroga cerealella in the granary (정부양곡창고내에서 딜과 머그워트 오일을 이용한 어리쌀바구미와 보리나방에 대한 살충 실증실험)

  • Song, Jaeun;Lee, Hoi-Seon
    • Journal of Applied Biological Chemistry
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    • v.60 no.1
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    • pp.1-3
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    • 2017
  • This study was conducted to evaluate the insecticidal effects of the Anethum graveolens and the Artemisia vulgaris oils against Sitophilus zeamais and Sitotroga cerealella, respectively, in granary. Insecticidal effects of the A. graveolens oil showed 100 % mortality against S. zeamais for 5 days. In the case of S. cerealella, the A. vulgaris oil showed 100 % mortality for 2 h. These results indicate that the A. graveolens and A. vulgaris oils have potential for development as insecticidal to control S. zeamais and S. cerealella, respectively.

Biological Activities of Yellow Garlic Extract (황마늘 추출물의 생리활성)

  • Kang, Jae Ran;Hwang, Cho Rong;Sim, Hye Jin;Kang, Min Jung;Kang, Sang Tae;Shin, Jung Hye
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.44 no.7
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    • pp.983-992
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    • 2015
  • This study investigated the quality characteristics and biological activities, such as antioxidant, whitening, anti-diabetes, and anti-inflammatory activities, of yellow garlic, by simplify processing time and manufacturing process compared with black garlic. Extracts were prepared various ratios of water and ethanol solvent [water : ethanol (v/v)=100:0, 70:30, 50:50, 30:70, 0:100] from yellow garlic. Alliin content of yellow garlic showed no difference compared with fresh garlic, whereas S-allyl cysteine content of yellow garlic was higher than that of fresh garlic. Alliin content of yellow garlic extracts increased in an ethanol concentration-dependent manner. Total phenol and flavonoid contents were highest in 100% ethanol extract. DPPH and ABTS radical scavenging abilities did not show significant differences among 0~70% ethanol extracts, whereas 100% ethanol extract showed the highest contents of 93.45% and 91.46%, respectively. Tyrosinase and ${\alpha}$-glucosidase inhibitory activities were also highest in 100% ethanol extract, but did not show significant differences among the extract solvents. Water and ethanol extracts from yellow garlic showed anti-inflammatory effects by modulating production of NO and cytokines at a concentration of $100{\mu}g/mL$. We suggest that yellow garlic has antioxidant, whitening, anti-diabetes, and anti-inflammatory activities and can be used as a functional material similar to black garlic.

Antioxidant and Antimicrobial Activities of Fruiting Bodies of Phellinus gilvus Collected in Korea (국내에서 수집된 마른진흙버섯 자실체의 항산화 및 항균 효과)

  • Yoon, Ki-Nam;Jang, Hyung Seok
    • Korean Journal of Clinical Laboratory Science
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    • v.48 no.4
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    • pp.355-364
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    • 2016
  • This study was initiated to evaluate the antioxidant and antimicrobial activities of methanol extract (ME) and hot water extract (HWE) obtained from the fruiting bodies of medicinal mushroom, Phellinus gilvus. The free radical scavenging activity of ME from P. gilvus on 1,1-diphenyl-2-picrylhydrazyl (DPPH) were 93.65% at 2 mg/mL, which was comparable with the positive control, butylated hydroxytoluene (BHT, 96.97%) at the same concentration. The ferrous ion-chelating ability of ME and HWE was significantly higher than that of BHT at all concentration levels. The antimicrobial assay of ME was performed against six bacteria and one species of fungus. ME exhibited antibacterial activity against 5 out of 6 bacteria: Staphylococcus aureus, Streptococcus mutans, Bacillus subtilis, Escherichia coli, and Pseudomonas aeruginosa; whereas, ME did not show antimicrobial activity against gram-negative bacterium Vibrio vulnificus and fungal species Candida albicans. The minimum inhibitory concentration (MIC) of ME against 5 strains of bacteria, such as S. aureus, S. mutans, B. subtilis, E. coli, and P. aeruginosa, was 100, 100, 50, 100, 200 mg/mL, respectively. The results suggest that good antioxidant and microbial activities of P. gilvus fruiting bodies might be used for natural antioxidant and antimicrobial agents.

Formation of Fine Pitch Solder Bumps on Polytetrafluoroethylene Printed Circuit Board using Dry Film Photoresist (Dry Film Photoresist를 이용한 테프론 PCB 위 미세 피치 솔더 범프 형성)

  • 이정섭;주건모;전덕영
    • Journal of the Microelectronics and Packaging Society
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    • v.11 no.1
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    • pp.21-28
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    • 2004
  • We have demonstrated the applicability of dry film photoresist (DFR) in photolithography process for fine pitch solder bumping on the polytetrafluoroethylene (PTFE/Teflon ) printed circuit board (PCB). The copper lines were formed with 100$\mu\textrm{m}$ width and 18$\mu\textrm{m}$ thickness on the PTFE test board, and varying the gaps between two copper lines in a range of 100-200$\mu\textrm{m}$. The DFRs of 15$\mu\textrm{m}$ thickness were laminated by hot roll laminator, by varying laminating temperature from $100{\circ}C$ to 15$0^{\circ}C$ and laminating speed from 0.28-0.98cm/s. We have found the optimum process of DFR lamination on PTFE PCB and accomplished the formation of indium solder bumps. The optimum lamination condition was temperature of $150^{\circ}C$ and speed of about 0.63cm/s. And the smallest size of indium solder bump was diameter of 50$\mu\textrm{m}$ with pitch of 100$\mu\textrm{m}$.

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The influence of Procyanidins, Gallic acid and Theaflavins extracted level when fermented sun-dried salt and green tea (천일염을 이용한 녹차의 발효가 Procyanidins와 Gallic acid 및 Theaflavins 추출량에 미치는 영향)

  • Yoon, Hyun;Choi, Cheol-Won
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.15 no.6
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    • pp.3774-3780
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    • 2014
  • This study examined green tea fermented by the addition of sun-dried salt to increase the extraction of Procyanidins and Gallic acid and Theaflavins. An experiment on the change in the amount of the extract according to the saturation degree of sun-dried salt was performed. The sample of fermented only green tea extracted only Gallic acid (0.004 mg/g). No Procyanidins and Theaflavins were extracted. When the green tea was fermented by sun-dried salt, large amounts of the three compounds were extracted: Procyanidins, 75%(0.244 mg/g) and 100%(0.949 mg/g); Gallic acid, 75%(0.386 mg/g) and 100%(0.691 mg/g), and Theaflavins, 75%(0.083 mg/g). According to the date of fermentation, the largest amount of Procyanidins, Gallic acid and Theaflavins extracted were on the 2nd day(0.295 mg/g) and 7th day(2,256 mg/g) and 4th day(0.168 mg/g), respectively.

MEIS를 이용한 Cu3Au(100)의 Surface Induced disorder 직접관찰

  • 오두환;강희재;채근화;김현경;문대원
    • Proceedings of the Korean Vacuum Society Conference
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    • 1999.07a
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    • pp.179-179
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    • 1999
  • Cu3AU(100) 단결정은 fcc 구조를 가지고 있으며 (100)면은 Cu와 Au가 1:1로 존재하고 가운데(200)면은 Cu만 존재한다. 따라서 Au 층은 (100)면에서만 존재하여 각 Au층은 서로 0.5nm 떨어져 있다. 이와 같은 Cu3Au(100) 단결정을 MEIS(Medium Energy Ion Scattering Spectroscopy) 실험 장비를 사용하여 0.35nm 떨어져 있는 Single unit cell의 윗면과 아래면, 즉 첫 층의 Au와 셋째층의 Au의 층분리를 통해서, 온도 변화에 따른 Cu3Au(100) 단결정의 표면 물리적 현상인 surface induced disorder을 밝혀내고자 한다. 우선 두 Au층의 분리 시도는 수소이온을 이용한 실험 조건에서는 extremely glancing exit angle 등 극한의 산란조거에서도 성공하지 못하였다. 깊이 분해능을 정해주는 electronic energy loss를 극대화하기 위해 수소이온이 아닌 질소 이온을 사용하여 energy spectra를 측정해 본 결과 아래 그림에서와 같이 표면 Au 층과 표면 셋째 Au층을 구분할 수 있었다. <110>으로 align된 조건에서는 셋째층의 Au 원자들이 완전히 shadow cone 내부에 존재하여 관측되지 않지만 9.75$^{\circ}$ tilt 한 경우 셋째층의 Au 원자들이 shadow cone 바깥으로 나오게 되어 그림에서와 같이 첫째 층과 셋째 층이 확실히 분리되어 측정되었다. 이를 바탕을 Cu3Au(100)의 온도변화에 다른 order disorder and segregation 현상을 측정하였다. ordered Cu3Au(100)은 28$0^{\circ}C$ 근처에서 surface층이 먼저 disordered상으로 바뀌는 surface induced disorder 현상이 일어나고 bulk transition 온도 39$0^{\circ}C$ 이하에서 R.T으로 온도를 낮추면 본래의 ordered 구조로 되돌아간다. 하지만 bulk transition 온도를 지나면 order-disorder transition이 비가역적이고 segregation 현상이 일어난다.

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The study of masan barber's coastal line change during 100years

  • Choi, Chul-Uong;Kim, Young-Seop;Cho, Sung-Hwan
    • Proceedings of the KSRS Conference
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    • 2002.10a
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    • pp.273-273
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    • 2002
  • Masan barber was situated in S.Korean southern central coast. And it is contributing greatly in S.korea's economy development to international trading port of Heaven's blessing that possess natural, geographical situation. Also, because there are Masan free tax area and chanwon heavy industrial complex, sachon air industrial complex etc. on back, it is important permanency in our country. Specially, because inland transport routes such as southern highway, Guma highway and national road system are developed well, the importance is very high. Masan harbor 1899.05.01 be that opened, the 1st and 2nd (central pier) was build 1938∼1944 year, and the 3rd pier was 1973∼1978 year. the 4th pier was 1974∼1983 year, 5th pier was 1984∼1988, 6th pier (west pier) was 1985∼1992 year. it was developed over 100 years. But, it did great many harbor and bay development of last 100 yens but research about coastline change and seashore reclamation is insufficient. Therefore, this research executed research about coastline change of Masan bay of last 100 years, In this study, we analyzed aerial photographs and tide data for the past 100 years using digital aerial photo analysis and GIS techniques for each 3-year interval. We abstracted beach DEM (digital elevation model) and ortho aerial photographs, and conducted a space analysis. As a result, we were able to identify changes in the area As a result, we drew 10 years cycle coastline change of Masan bay. and we can detect bay coastal line change and calculate refill rate.

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S100A16 is a Prognostic Marker for Lung Adenocarcinomas

  • Saito, Keita;Kobayashi, Makoto;Nagashio, Ryo;Ryuge, Shinichiro;Katono, Ken;Nakashima, Hiroyasu;Tsuchiya, Benio;Jiang, Shi-Xu;Saegusa, Makoto;Satoh, Yukitoshi;Masuda, Noriyuki;Sato, Yuichi
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.16
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    • pp.7039-7044
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    • 2015
  • Background: Many functional molecules controlling diverse cellular function are included in low-molecular weight proteins and peptides. Materials and Methods: To identify proteins controlling function in lung adenocarcinomas (AC), we performed two-dimensional gel electrophoresis employing tricine-SDS polyacrylamide in the second dimension (tricine 2-DE). This system was able to detect proteins under 1 kDa even with post-translational modifications. To confirm the utility of detected proteins as novel tumor markers for AC, we performed immunohistochemical analysis using 170 formalin-fixed and paraffin-embedded lung AC tissues. Results: Tricine 2-DE revealed that five proteins including S100A16 were overexpressed in lung AC-derived cells compared with lung squamous cell carcinoma, small cell carcinoma, and large cell neuroendocrine carcinoma-derived cells. Immunohistochemically, S100A16 showed various subcellular localization in lung cancer tissues and a membranous staining status was correlated with the T-factor (P=0.0008), pathological stage (P=0.0015), differentiation extent (P=0.0001), lymphatic invasion (P=0.0007), vascular invasion (P=0.0001), pleural invasion (P=0.0087), and gender (P=0.039), but not with the age or smoking history. More importantly, membranous staining of S100A16 was significantly correlated with a poorer overall survival of either stage I (P=0.0088) or stage II / III (P=0.0003) lung AC patients, and multivariate analysis confirmed that membranous expression of S100A16 was an independent adverse prognostic indicator (P=0.0001). Conclusions: The present results suggest that S100A16 protein is a novel prognostic marker for lung AC.

E. faecalis and E. faecium Isolated in Dried Marine Products (시판 건해산물에서 분리한 Enterococcus faecalis와 E. faecium의 미생물학적 특성)

  • Ham, Hee-Jin
    • Journal of Food Hygiene and Safety
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    • v.22 no.4
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    • pp.294-299
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    • 2007
  • Eighty seven strains were isolated from 164 dried marine products(dried squid and dried alaska pollack etc) in Seoul Garak wholesale market. Among 87 isolates, twenty four E. faecalis and 4 E. faecium were identified by API strep kit. Twenty eight strains of E. faecalis, and E. faecium were resistant in streptomycin (95.6%), kanamycin (84.5%), gentamycin (66.7%), cephaloxin (97.8%), ampicillin/sulbactam (88.9%), ticarcillin(66.7%), amikacin (97.8%), sulfonamides (97.8%), ceftriaxone (75.6%), nalidixic acid (100.0%), and cefoxitin (100.0%), and were susceptible in amoxicillin/clavulanic acid(97.8%), chloramphenicol(95.6%), sulfamethoxazole/trimethoprim (97.8%), and tetracycline (71.1%). Also, ten strains of E. faecalis was resistant in $S-K-GM-CF-SAM-TIC-An-S_3-CRO-NA-FOX$ drugs simultaneously. Conclusively, E. faecalis strains from dried marine products were resistant on antibiotic drugs residue.

Investigation of adsorption structure for methionine on Ge(100)

  • Yang, Se-Na;Yun, Yeong-Sang;Park, Seon-Min;Hwang, Han-Na;Hwang, Chan-Guk;Kim, Se-Hun;Lee, Han-Gil
    • Proceedings of the Korean Vacuum Society Conference
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    • 2010.02a
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    • pp.369-369
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    • 2010
  • Adsorption and ordering of methionine molecules on Ge(100) surface have been studied using high resolution photoemission spectroscopy and low-energy electron diffraction (LEED) to investigate the adsorption structure as a function of coverage. Analysis of C 1s, S 2p, N 1s, and O 1s core levels reveals quite different according to methionine coverage. We found that the relative population of the two types of thiolates induces a structural change in the ordering from $2\;{\times}\;1$ to $1\;{\times}\;1$. Such an unusual evolution of the methionine adsorption on the Ge(100) surface is discussed in relation to chemical reactions and possible molecular rearrangement on the surface.

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