• Title/Summary/Keyword: ammonia treatment

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Effects of Mustard Seeds and Powder on In vitro Ruminal Fermentation Characteristics and Methane Production (겨자종자와 겨자분의 첨가가 반추위 발효성상과 메탄생성에 미치는 영향)

  • Lee, Kang Yeon;Kim, Kyoung Hoon;Baek, Youl Chang;Ok, Ji Un;Seol, Yong Joo;Han, Ki Jun;Park, Keun Kyu;Ryu, Ho Tae;Lee, Sang Suk;Jeon, Che Ok;Oh, Young Kyoon
    • Journal of Animal Science and Technology
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    • v.55 no.1
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    • pp.25-32
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    • 2013
  • The purpose of this study was to investigate the effects of mustard, which contains allyl isothiocyanate, on ruminal fermentation and methane emission in vitro. To this end, diluted ruminal fluid(30ml) was incubated anaerobically at $39^{\circ}C$ or 6, 12, and 24 h with or without seeds or powdered mustard. Either mustard seed or powdered mustard was weighed and serially (0, 3.33, 5.00, 6.67, and 8.33 g/L) mixed with ruminal fluid. Ammonia-N was increased (P < 0.05) by mustard treatment in a dose dependent manner. Regardless of concentration or form, mustard increased (P < 0.05) total VFA content but decreased (P < 0.01) pH compared to control group. Molar proportion of acetate (A) was decreased (P < 0.05) whereas propionate (P) was increased (P < 0.05) by mustard treatment, thereby A:P ratio was decreased (P < 0.05) compared to control group. Total gas production was increased (P < 0.01) in a linear manner by mustard treatment compared to control group. There was no effect of mustard powder, except 8.33 g/L level at 6 h, on methane emission. However, at 24 h, methane emission was reduced (P < 0.05) by 4.77% and 11.54% with 6.67 g/L and 8.33 g/L of mustard seeds supplementation, respectively. Altogether, these results suggest that mustard seeds containing allyl isothiocyanate may reduce methane production without disturbing ruminal fermentation.

Factors Affecting the Formation of Iodo-Trihalomethanes during Chlorination in Drinking Water Treatment (정수처리에서 염소 처리시 요오드계 트리할로메탄류 생성에 영향을 미치는 인자들)

  • Son, Hee-Jong;Yoom, Hoon-Sik;Kim, Kyung-A;Song, Mi-Jeong;Choi, Jin-Taek
    • Journal of Korean Society of Environmental Engineers
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    • v.36 no.8
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    • pp.542-548
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    • 2014
  • Effects of bromide ($Br^-$) and iodide ($I^-$) concentrations, chlorine ($Cl_2$) doses, pH, temperature, ammonia nitrogen concentrations, reaction times and water characteristics on formation of iodinated trihalomethanes (I-THMs) during oxidation of iodide containing water with chlorine were investigated in this study. Results showed that the yields of I-THMs increased with the high bromide and iodide level during chlorination. The elevated pH significantly increased the yields of I-THMs during chlorination. The formation of I-THMs was higher at $20^{\circ}C$ than $4^{\circ}C$, $10^{\circ}C$ and $30^{\circ}C$. In chloramination study, addition of ammonium chloride ($NH_4Cl$) markedly increased the formation of I-THMs. Among the water samples collected from seven water sources including wastewater treatment plant (WWTP) effluent water (EfOM water), prepared humic containing water (HA water) and algal organic matter (AOM) containing water (AOM water), EfOM water generated the highest yields of I-THMs ($12.31{\mu}g/mg$ DOC), followed by HA water ($4.96{\mu}g/mg$ DOC), while AOM water produced the lowest yields of I-THMs ($0.99{\mu}g/mg$ DOC). $SUVA_{254}$ values of EfOM water, HA water and AOM water were $1.38L/mg{\cdot}m$, $4.96L/mg{\cdot}m$ and $0.97L/mg{\cdot}m$, respectively. The I-THMs yields had a low correlation with $SUVA_{254}$ values ($r^2$ = 0.002).

Utilization of Wood Chips for Disposing of Swine Manure (목질칩의 축분뇨 정화재로의 이용)

  • Choi, In-Gyu
    • Korean Journal of Environmental Agriculture
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    • v.20 no.4
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    • pp.203-210
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    • 2001
  • In order to environmentally use wood chips manufactured from low valued forest resources by forest tendering, wood chips were used for the evaluation on chips characteristics, decomposition capability of organic wastes, and field experiment and determination of conditions for decomposer. Bioclusters manufactured by Cryptomeria japonica, commercially available wood chips in Japan, showed higher pore ratio, water reservation and water resistance, and higher cellulose content with lower hot water solubles than domestic wood chips. The useful size of wood chips for swine manure decomposition was 10 (length) ${\times}$ 5 (width) ${\times}$ 2 (thickness) mm, and cellulose contents and alkali solubles of Pinus densiflora and Populus tomentiglandulosa were similar to those of bioclusters. According to the decomposition ratio depending on wood species, it was ordered as Pinus densiflora > Pinus koraiensis > Cryptomeria japonica. The swine manure decomposition ratio depending on treatment hours by Pinus koraiensis was constant with the ratio of 15 to 16 g per hour by 1 kg of chip, indicating of daily swine decomposition amount of 390 kg by 1 ton of chips which was equal to the amount of daily swine manure production by 70 swines. Analyzing by long term used wood chips during 40 days treatment, the treated wood chips characteristically showed stable total nitrogen content, suitable pH, high accumulation of inorganic contents such as calcium, phosphorus, potassium and sodium, and no odor. During winter, the inner temperature of decomposer was kept at $43^{\circ}C$, but air bubble was occurred due to high pH and viscosity of swine manure. The most appropriate mixing ratio between wood chips and swine manure was 1 versus 2 or 3, and at more than ratio 1 versus 3, ammonia gas was caused because of anaerobic fermentation status by high moisture content of wood chips. The mixing interval of decomposer was 3 mins. per hour for the best swine decomposition.

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Effects of Supplemental Synbiotics Composed of Anaerobic Bacteria, Yeast and Mold on the Change of Chemical Composition and Fermentation Characteristics of Total Mixed Ration for Cattle (혐기성 박테리아, 효모 및 곰팡이로 제조된 synbiotics 첨가 축우용 완전혼합사료의 성분 변화 및 발효 특성에 미치는 영향)

  • Lee, Shin-Ja;Shin, Nyeon-Hak;Jung, Ho-Sik;Moon, Yea-Hwang;Lee, Sang-Suk;Lee, Sung-Sill
    • Journal of Life Science
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    • v.19 no.2
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    • pp.241-248
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    • 2009
  • In order to investigate the effects of synbiotics on change of chemical composition and fermentation characteristics of total mixed ration (TMR), eight TMRs fermented by synbiotics composing the anaerobic microbes (bacteria, yeast, mold) were alloted to the experimental treatments. Treatments were composed of untreated synbiotics(US), bacterial synbiotics (BS), yeast synbiotics (YS), mold synbiotics (MS), bacterial and mold synbiotics (BMS), yeast and mold synbiotics (YMS), bacterial and yeast synbiotics (BYS), and bacterial, yeast and mold synbiotics (BYMS). After 7 days of anaerobic fermentation, fermented-TMRs were exposed to air during 1, 3, 5, 7, 14 and 21 days. One hundred forty four (8 treatments ${\times}$ 6 exposing days ${\times}$ 3 replications) fermented- TMRs were manufactured by vinyl bag sized of 43 cm by 58 cm. The results obtained were as follows. Moisture contents of the fermented TMRs anaerobically ranged from 41% to 45%, and was similar to those of basal TMRs. As results of anaerobic fermentation, the concentration of crude protein was decreased by 11.7% to 14.8% in the untreated sample, while was rather increased by 11% when the TMR was fermented with BMYS. And also BMYS treatment showed decreases by 32% for crude fiber, 15.5% for NDF and 26.1% for ADF. Internal temperature of fermented-TMRs was highest at 7 day of exposing in the air. The pH of fermented-TMR juice was significant difference betweentreatments after 7 day of exposing in air, and that of BMS was highest at 14 day after exposing in air (P<0.05). Acid buffering capacity was increased in proportion to the exposing day of TMR, and peaked at 7 or 14 days after exposing. Ammonia concentration of fermented-TMRs was highest at 5 day after exposing in the air. Individual volatile fatty acid of fermented-TMR juice was very low level in all treatments. Although BMYS treatment to TMR inclined to increase in crude protein and decrease in fibers, but there were no positive effects on the fermentation characteristics after exposing in the air by supplementation of anaerobic synbiotics to TMR.

Effects of the Additives on the Quality of Alfalfa Silage (첨가제 처리가 알팔파 사일리지의 품질에 미치는 영향)

  • Kim, J.G.;Chung, E.S.;Kang, W.S.;Ham, J.S.;Kim, J.D.;Seo, S.;Lee, J.K.
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.19 no.2
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    • pp.115-120
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    • 1999
  • This experiment was carried out to investigate the effects of additives on the quality of alfalfa(Medicago sativa L.) silage at the forage experimental field, National Livestock Research Institute, RDA, Suwon in 1996. The experiment was arranged in a randomized block design with three replications. The treatments consisted of different additives(control, formic acid, molasses, inoculant A and inoculant B). Crude protein(CP) and Nitrogen free extract(NFE) content of alfalfa silage with additives were higher compared with those obtained control, but Crude ash(CA) and Crude fiber(CF) content of control(11.5 and 39.6%) were higher than those of additive treated plots. ADF and NDF contents of control silage were the highest as 36.2 and 48.6%, respectively, and increased by 4% compared with those obtained before ensiling. The acidity(pH) of control was the highest(5.45), but the lowest in inoculant A(4.32). Inoculant teratment significantly reduced acidity of silage compared with control. DM content of control silage was the lowest but DM loss of control silage was higher than that of additive treated silage. Ammonia-N content was the highest at formic acid treatment and level of Lactobacillus was decreased at control and formic acid treated silage. Total organic acid and lactic acid content of inoculant A and inoculant B were higher than those of other treated plots. The results of this study indicate that the treatment of LAB(Lactic acid bacteria) could be recommended as producing high quality of alfalfa silage.

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Effects of Yucca Extracts and Protein Levels on Growth Performance, Nutrient Utilization and Carcass Characteristics in Finishing Pigs

  • Min, T.S.;Kim, J.D.;Lee, J.H.;Hyun, Y.;Sohn, K.S.;Han, In K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.4
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    • pp.525-534
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    • 2001
  • A total of 120 pigs were used to investigate the effects of yucca extracts on the growth performance, nutrient digestibility, nutrient excretion and carcass characteristics of finishing pigs fed different levels of dietary protein. Pigs were allotted into $2{\times}3$ factorial design by the supplementation of yucca extracts (YE, 0 and 120 mg/kg) and 3 levels of dietary protein (14, 16, 18% for early finisher and 12, 14, 16% for late finisher for low, medium and high protein diet, respectively). During the early finishing period (51~76 kg BW), no significant difference was found in growth performance regardless of the YE supplementation or dietary protein levels. Growth performance of late finishing pigs (76~101 kg BW) was also not significantly different among treatments. However, ADG of pigs fed YE diet was significantly improved (p<0.05) regardless of the dietary protein levels. For the overall period (51~101 kg BW), although adding YE to the diet and elevating the protein level showed better ADG, there were no significant differences on growth performance among treatments. Early finishers showed significantly higher crude protein, crude ash and crude fat digestibilities when they were fed diets supplemented with YE. Digestibilities of amino acids were not affected by YE. Late finishers did not show any significant differences in proximate nutrient digestibilities regardless of YE supplementation or dietary protein levels. YE tended to slightly improve the CP digestibility, however no significant difference was found with increased dietary protein levels. There was no significant difference in amino acid digestibilities with YE supplementation or dietary CP levels during the late finishing period. Dry matter (DM) and nitrogen (N) excretion in feces did not show any significant difference among treatments. Early finishing pigs also did not respond to the inclusion of YE or dietary protein levels (p<0.05). Fecal N excretion of early finishing pigs seemed to be lowered in pigs fed YE. Pigs fed medium dietary protein diet tended to excrete a higher amount of N during the early finishing period, but not statistically different. A slight increase in fecal N excretion was found with the increased level of dietary protein during the late finishing period. For ammonia nitrogen excretion, although there was no significance, the NH3-N content tended to be increased by the increased dietary protein levels and with YE supplementation. The NH3-N content in manure increased by 24.5% with YE supplementation. There were no significant differences in carcass weight, backfat thickness, carcass grade and loin eye area among treatments. However, pigs fed non-YE with low protein diet showed a significantly (p<0.05) low carcass ratio among treatments and there was significant (p<0.05) difference between the YE-added treatment and non YE treatment in carcass ratio. As for the feed cost, the cost of feeding high level protein was higher than that of medium level protein by 5% and low level protein by 9% (p<0.05). Therefore, based on this study, it could be concluded that environmentally friendly agents might play a role to some extent in finishing pigs from the aspect of pollution control, and that more than 14 and 12% of dietary protein for early finishing and late finishing pigs respectively do not necessarily guarantee high growth performance.

Nutrient Utilization, Body Composition and Lactation Performance of First Lactation Bali Cows (Bos sondaicus) on Grass-Legume Based Diets

  • Sukarini, I.A.M.;Sastradipradja, D.;Sutardi, T.;Mahardika, IG.;Budiarta, IG.A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.13 no.12
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    • pp.1681-1690
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    • 2000
  • A study on energy and protein utilization, and milk production of Bali cows on grass-legume diets was carried out using 12 first lactation cows (initial BW $263.79{\pm}21.66kg$) during a period of 16 weeks starting immediately post calving. The animals were randomly allotted into 4 dietary treatment groups R1, R2, R3 and R4, receiving from the last 2 months of pregnancy onwards, graded improved rations based on a mixture of locally available grass and legume feed ad libitum. R1 contained on a DM basis 70% elephant grass (PP, Penisetum purpureum) plus 30% Gliricidia sepia leaves (GS), R2 was 30% PP plus 55% GS supplemented with 15% Hibiscus tilliactus leaves (HT, defaunating effect), R3 and R4 were 22.5% PP+41.25% GS+11.25% HT+25% concentrate, where R3 was not and R4 supplemented with zinc di-acetate. TDN, CP and zinc contents of the diets were 58.2%, 12.05% and 18.3 mg/kg respectively for R1, 65.05%, 16.9% and 25.6 mg/kg respectively for R2, 66.03%, 16.71% and 29.02 mg/kg respectively for R3 and 66.03%, 16.71% and 60.47 mg/kg respectively for R4. Milk production and body weight were monitored throughout the experimental period. In vivo body composition by the urea space technique validated by the body density method and supported by carcass data was estimated at the start and termination of the experiment. Nutrient balance and rumen performance characteristics were measured during a balance trial of 7 days during the 3rd and 4th week of the lactation period. Results indicated that quality of ration caused improvement of ruminal total VFA concentration, increments being 52 to 65% for R2, R3 and R4 above R1, with increments of acetate being less (31 to 48%) and propionate being proportionally more in comparison to total VFA increments. Similarly, ammonia concentrations increased to 5.24 to 7.07 mM, equivalent to 7.34 to 9.90 mg $NH_3-N/100ml$ rumen fluid. Results also indicated that feed quality did not affect DE and ME intakes, and heat production (HP), but increased GE, UE, energy in milk and total retained energy (RE total) in body tissues and milk. Intake-, digestible- and catabolized-protein, and retained-protein in body tissues and milk (Rprot) were all elevated increasing the quality of ration. Similar results were obtained for milk yield and components with mean values reaching 2.085 kg/d (R4) versus 0.92 kg/d (R1) for milk yield, and 170.22 g/d (R4) vs 71.69 g/d (R1), 105.74 g/d (R4) vs 45.35 g/d (R1), 101.34 g/d (R4) vs 46.36 g/d (R1) for milk-fat, -protein, and -lactose, respectively. Relatively high yields of milk production was maintained longer for R4 as compared to the other treatment groups. There were no significant effects on body mass and components due to lactation. From the relationship $RE_{total}$ (MJ/d)=12.79-0.373 ME (MJ/d); (r=0.73), it was found that $ME_{m}=0.53MJ/kgW^{0.75}.d$. Requirement of energy to support the production of milk, ranging from 0.5 to 3.0 kg/d, follows the equation: Milk Prod. ($Q_{mp}$, kg/d)=[-2.48+4.31 ME($MJ/kg^{0.75}.d$)]; (r=0.6) or $Q_{mp}$=-3.4+[0.08($ME-RE_{body\;tissue}$)]MJ/d]; (r=0.94). The requirement for protein intake for maintenance ($IP_m$) equals $6.19 g/kg^{0.75}.d$ derived from the relationship RP=-47.4+0.12 IP; (r=0.74, n=9). Equation for protein requirement for lactation is $Q_{nl}$=[($Q_{mp}$)(% protein in milk)($I_{mp}$)]/100, where $Q_{nl}$ is g protein required for lactation, $Q_{mp}$ is daily milk yield, Bali cow's milk-protein content av. 5.04%, and $I_{mp}$ is metabolic increment for milk production ($ME_{lakt}/ME_{m}=1.46$).

Effects of Probiotics as an Alternative for Antibiotics on Growth Performance, Nutrient Digestibility, Noxious Gas Emission and Fecal Microbial Population in Growing Piglets (항생제 대체 생균제가 자돈의 생산성,영양소 이용률, 유해가스 발생량 및 분내 미생물 수에 미치는 영향)

  • Kim, Jong-Duk;Chung, Heung-Woo;Shim, Kum-Seob;Park, Seung-Young;Ju, Jong-Cheol;Song, Jae-Jun;Lee, Kyung-Ho;Park, Joong-Kook;Park, Do-Yun;Kim, Chang-Hyun
    • Korean Journal of Organic Agriculture
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    • v.18 no.4
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    • pp.527-539
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    • 2010
  • This study was conducted to determine the effect of probiotics as an alternative for antibiotics on growth performance, nutrient digestibility, noxious gas emission and fecal microbial population in growing piglets. A total of 96 piglets ($22.5{\pm}1.3$kg average body weight) were allotted to 3 different treatment groups and replicated 4 times with 8 piglets per replicate in randomized complete block design. Treatments were T1) (Control, basal diet+0.2% antibiotics), T2) 0.2% probiotics complex and T3) 0.3% Bacillus probiotics. During the whole experiment period, there were no differences (p>0.05) in average daily gain (ADG), average daily feed intake (ADFI) and feed efficiency. However, digestibility of dry matter, crude protein, ether extract, nitrogen free extract and crude ash were showed higher in probiotics groups (T2 and T3) than those of control. In noxious gas emission, ammonia, amine, hydrogen sulfide and mercaptan were significantly (p<0.05) reduced in T2 and T3 treatments compared to those in control. Moisture content of feces was not significantly different among treatments. The colony forming units (CFU) of total bacteria, E. coli and thermoduric bacteria in feces were significantly different among treatments. The CFU of total bacteria, E. coli and thermoduric bacteria in T3 treatment were reduced by feeding probiotics B. From this study, we suggest that probiotics A and B are likely able to improve the growth performance and nutrients digestibility, reduce noxious gas emission and change the fecal microbial composition in growing piglets.

Study on the Relationship between Utilization of Nutrients in the Rumen and Rumen Microorganisms by Dietary Energy and Protein Source (에너지와 단백질(蛋白質) 공급원(供給源)에 의(依)한 반추위내(反芻胃內) 영양소(營養素) 이용성(利用性) 및 반추위미생물(反芻胃微生物)에 관(關)한 연구(硏究))

  • Chee, Sul Ha;Lee, Sang Cheol;Jeong, Ha Yeon
    • Korean Journal of Agricultural Science
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    • v.23 no.2
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    • pp.188-205
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    • 1996
  • This study was conducted to examine the effects of dietary energy and protein sources on the rumen microbial population of sheep. The results obtained were summarized as the follows; 1. Ruminal pH was highest in sheep fed the diet rapidly degraded in the rumen(F-F) as a energy (barley plus beet pulp) and protein source (rapeseed meal), and lowest in the diet (F-S) of rapidly degradable energy source plus slowly degradable protein source (corn gluten meal + cotton seed meal) without affecting by postfeeding time. 2. Ruminal ammonia concentration was higher in corn (slowly degradable) for energy source and rapeseed meal (rapidly degradable) for protein source (S-F) than others, and abruptly increased at 1 hr after feeding regardless of treatments. 3. Concentration of ruminal total volatile fatty acid, acetate and propionate were highest in F-S and peaked at 1 hr after feeding firstly and formed second peak at 9hrs, respectively. 4. Digestibilities of the proximates and ADF were not affected by treatment but NDF was highest in F- F. 5. Nitrogen retention was highest in S-F and lowest in F-F and F-S. 6. Digestibility and metabolizability of energy, contents of OCP, TDN, DE and ME were not affected by treatment.

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Development and Evaluation of Model-based Predictive Control Algorithm for Effluent $NH_4-N$ in $A^2/O$ Process ($A^2/O$ 공정의 유출수 $NH_4-N$에 대한 모델기반 예측 제어 알고리즘 개발 및 평가)

  • Woo, Dae-Joon;Kim, Hyo-Soo;Kim, Ye-Jin;Cha, Jae-Hwan;Choi, Soo-Jung;Kim, Min-Soo;Kim, Chang-Won
    • Journal of Korean Society of Environmental Engineers
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    • v.33 no.1
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    • pp.25-31
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    • 2011
  • In this study, model-based $NH_4-N$ predictive control algorithm by using influent pattern was developed and evaluated for effective control application in $A^2/O$ process. A pilot-scale $A^2/O$process at S wastewater treatment plant in B city was selected. The behaviors of organic, nitrogen and phosphorous in the biological reactors were described by using the modified ASM3+Bio-P model. A one-dimensional double exponential function model was selected for modeling of the secondary settlers. The effluent $NH_4-N$ concentration on the next day was predicted according to model-based simulation by using influent pattern. After the objective effluent quality and simulation result were compared, the optimal operational condition which able to meet the objective effluent quality was deduced through repetitive simulation. Next the effluent $NH_4-N$ control schedule was generated by using the optimal operational condition and this control schedule on the next day was applied in pilot-scale $A^2/O$ process. DO concentration in aerobic reactor in predictive control algorithm was selected as the manipulated variable. Without control case and with control case were compared to confirm the control applicability and the study of the applied $NH_4-N$control schedule in summer and winter was performed to confirm the seasonal effect. In this result, the effluent $NH_4-N$concentration without control case was exceeded the objective effluent quality. However the effluent $NH_4-N$ concentration with control case was not exceeded the objective effluent quality both summer and winter season. As compared in case of without predictive control algorithm, in case of application of predictive control algorithm, the RPM of air blower was increased about 9.1%, however the effluent $NH_4-N$ concentration was decreased about 45.2%. Therefore it was concluded that the developed predictive control algorithm to the effluent $NH_4-N$ in this study was properly applied in a full-scale wastewater treatment process and was more efficient in aspect to stable effluent.