• 제목/요약/키워드: amino acid analyzer

검색결과 97건 처리시간 0.028초

한국산목이(韓國産木耳)와 석의(石衣)의 중성지질(中性脂質)과 아미노산(酸)의 성분(成分)에 관(關)한 연구(硏究) (Approach to the Neutral Lipid and Amino Acid Components of Hirneola Auricula-Judae and Gyrophora Esculenta in Korea)

  • 남정원;고영수
    • Journal of Nutrition and Health
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    • 제13권2호
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    • pp.92-103
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    • 1980
  • 한국산(韓國産) 목이(木耳)버섯과 용도(用途)가 비슷한 석의(石衣)버섯 중(中)의 중성지질(中性脂質)의 성분(成分)을 Thinchrography(latroscan TH-10) 에 의(依)해서 그리고 총(總)아미노산(酸)의 조성(組成)을 아미노산(酸) 자동분석기(自動分析機)(Technicon PNC-1 Type)에 의(依)해서 정량(定量) 분석(分析)을 한 결과(結果) 다음과 같은 결론(結論)을 얻었다. 1) 목이(木耳)와 석의중(石衣中)의 중성지질(中性脂質) 중(中)에는 cholesterolester와 tripalmitin이 주성분(主成分)임이 밝혀졌다. 2) 총(總) amino 산(酸)의 경우는 aspartic acid의 함량(含量)이 목이(木耳)에는 제일(第-) 많았으며 그 다음으로 glutamic acid, serine, alanine과 valine의 순(順)이었고 석의(石衣)의 경우에는 glutamic acid의 함량(含量)이 가장 높았다.

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Proteomic analysis of amino acid metabolism differences between wild and cultivated Panax ginseng

  • Sun, Hang;Liu, Fangbing;Sun, Liwei;Liu, Jianzeng;Wang, Manying;Chen, Xuenan;Xu, Xiaohao;Ma, Rui;Feng, Kai;Jiang, Rui
    • Journal of Ginseng Research
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    • 제40권2호
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    • pp.113-120
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    • 2016
  • Background: The present study aimed to compare the relative abundance of proteins and amino acid metabolites to explore the mechanisms underlying the difference between wild and cultivated ginseng (Panax ginseng Meyer) at the amino acid level. Methods: Two-dimensional polyacrylamide gel electrophoresis and isobaric tags for relative and absolute quantitation were used to identify the differential abundance of proteins between wild and cultivated ginseng. Total amino acids in wild and cultivated ginseng were compared using an automated amino acid analyzer. The activities of amino acid metabolism-related enzymes and the contents of intermediate metabolites between wild and cultivated ginseng were measured using enzyme-linked immunosorbent assay and spectrophotometric methods. Results: Our results showed that the contents of 14 types of amino acids were higher in wild ginseng compared with cultivated ginseng. The amino acid metabolism-related enzymes and their derivatives, such as glutamate decarboxylase and S-adenosylmethionine, all had high levels of accumulation in wild ginseng. The accumulation of sulfur amino acid synthesis-related proteins, such as methionine synthase, was also higher in wild ginseng. In addition, glycolysis and tricarboxylic acid cycle-related enzymes as well as their intermediates had high levels of accumulation in wild ginseng. Conclusion: This study elucidates the differences in amino acids between wild and cultivated ginseng. These results will provide a reference for further studies on the medicinal functions of wild ginseng.

韓國人蔘의 年根別 및 貯藏期間別 아미노酸分析 (A Study on the Analysis of Amino Acids in Korean Ginseng)

  • Rhee, Seong-Hong;Zong, Moon-Shik
    • 한국환경보건학회지
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    • 제9권2호
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    • pp.37-53
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    • 1983
  • The contents of amino acids were examined in the 3, 4, 5, and 6 year-old roots of fresh ginseng and the 1979, 1980, 1981, and 1982 years' products of white and red ginsengs. Samples extracted with 75% ethanol for free amino acids and hydrolyzed with 6N-HCL for total amino acids were analyzed by Amino Acid Analyzer (Hitachi model KLA-5). The results were summarized as follows: 1. Amino acids from extracted samples were 18 kinds of Tryptophan, Lysine, Histidine, Arginine, Aspartic acid, Threonine, Serine, Glutamic acid, Proline, Glycine, Alanine, Cystine, Valine, Methionine, Isoleucine, Leucine, Tyrosine, and Phenylalanine. 2. Amino acids detected in hydrolyzed samples were 17 kinds execpt Tryptophan of extracted ones. 3. Arginine was the highest quantity of amino acids in ginseng. 4. The content of Tryptophan was 0.5690 mg/g in the 6 year-old fresh ginseng and trace quantities in other samples. 5. The contents of amino acids were increased in fresh ginseng according to cultivation year. 6. The contents of amino acids in white ginseng were slightly decreased but those in red ginseng were not changed during the storage time. 7. The content ratio of free amino acids to total amino acids were 1:3.

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발효단백질의 유리아미노산 정량 (Conditions of Quantitative Analysis for free Amino Acid in Fermented Proteins)

  • 류홍수;문정혜;이강호
    • 한국식품영양과학회지
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    • 제17권2호
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    • pp.136-143
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    • 1988
  • 발효단백질 중의 유리아미노산을 효과적으로 정량하기 위하여 정량방법(추출조건, 제단백(除蛋白) 및 발색법)에 따른 영향을 검토하였다. 낙농발효품의 수용성 유리아미노산은 $75^{\circ}C$, 40분, 대두발효품은 $40^{\circ}C$, 3시간에서 효과적으로 추출되었다. 여러 침전방법(95% ethanol 처리, $100^{\circ}C$, 3분 가열 및 1% picric acid phthaldialdehyde로 발색시켜 D.L.-lysine을 표준아미노산으로 삼아 정량, 계산하였을 때가 아미노산자동분석기에 의한 결과에 가장 접근하였으나 ethanol로 침전시킨 뒤 OPDA로 발색시킨 결과도 이와 유사하였다. TNBS(2,4,6-trinitrobenzensulfonic acid)에 의한 발색정량에는 ethanol 침전처리가 가장 효과적이었으나 종래의 동감법(銅監法)(copper salt method, Spies and Chamber, 1958.)에 의한 결과는 다른 비색정량 및 아미노산자동분석기에 의한 결과에 비하여 시료에 따른 과소의 차이가 심하였고 재현성이 결여되었다.

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Ixeris속 식물의 약화학적 연구 2. 고들빼기의 플라보노이드 성분과 유리 아미노산 조성 (The Pharmaco-chemical Study on the Plant not Ixeris spp. 2. Flavonoids and Free Amino Acid Composition of Ixeris sonchifolia)

  • Young, Han-Suk;Im, Kwang-Sik;Park, Jae-Sue
    • 한국식품영양과학회지
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    • 제21권3호
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    • pp.296-301
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    • 1992
  • 고들빼기 잎으로부터 플라보노이드인 luteolin, luteolin 7-0-glucoside 및 apigenin 7-0-glucuronide를 분리하고 그들의 구조를 기기적인 분석방법에 의하여 동정하였으며 또한 유리 아미노산과 관련 화합물들의 조성과 상대함량을 표준품과 동일조건하에서 아미노산 자동분석기로 비교 검토하였다. 가장 함량이 많은 유리 아미노산들은 glutamic acid, aspartic acid, serine, proline, valine 그리고 arginine이었다.

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식이내의 타우린 또는 글라이신 보강이 흰쥐의 혈장과 간의 유리아미노산 농도 및 패턴에 미치는 영향 (Effect of Dietary Taurine or Glycine Supplementation on Plasma and Liver Free Amino Acid Concentrations in Rats)

  • 박정은
    • Journal of Nutrition and Health
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    • 제31권2호
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    • pp.126-134
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    • 1998
  • Our previous study demonstrated that dietary taurine or glycine supplementation significantly lowered plasma and hepatic cholesterol and triglyceride concentrations in rats fed a cholesterol-free diet. In the present study, the effect of long term dietary taurine or glycine supplementation, for the purpose of preventing and/or treating of hyperlipidemia and other known biological functions, on plasma and hepatic free amino acid concentrations and profiles were evaluated in rats. Three groups of male rats(110-130g) were fed a control diet(CD), taurine-supplemented diets(TSD ; CD+ 1.5% taurine) or glycine-supplemented diet(GSD ; CD+1.5% glycine) for 5 weeks. Plasma and hepatic free amino acid concentrations were determined by an automated amino acid analyzer based on ion-exchange chormatography. The feeding of TSD for 5 weeks yielded a 444% higher plasma taurine concentration , and the feeding GSD for the same period resulted in a 143% higher plasma glycine level in rats compared to those fed DB. Hepatic taurine concentration was significantly higher in rats fed TSD(145% increase) compared to the control rats. However, hepatic glycine concentration was not influenced by dietary glycine supplementation , which implies that the massive dose of glycine entering the body was more rapidly metabolized or excreted than taurein. Dietary taurine or glycine supplementation resulted in similar changes in plasma free amino acid concentrations, except in levels of taurine and glycine. Plasma levels of histidine, lysine, phenylalanine , alanine, proline, hydroxypoline, $\alpha$-aminogutyric acid, cystathionine and ethanolamine were significantly higher in rats fed TSD or GSD than those fed GD. Glycine supplementation did not change hepatic free amino acid concentrations as compared to CD. Concentrations of most hepatic free amino acids were not influenced by dietary taurine supplementation with the exception of significantly higher levels of asparate and tyrosine(56-63% increase) and lower levels of histidine and glutamate(33-34% decrease) compared to the control rats. These results suggest long-term dietary taurine or glycine supplementation resulted in increases in most plasma free amino acid levels, but did not cause a characteristic change in plasma aminogram pattern compared to rats fed CD.

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Studies on the Constituents of Higher Fungi of Korea (XX)

  • Lee, Man-Hyong;Choi, Eung-Chil;Kim, Byong-Kak
    • Archives of Pharmacal Research
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    • 제2권2호
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    • pp.133-144
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    • 1979
  • To investigate constituents of Strobilomyces floccopus (Fr.) Karst. and Coprinus comatus (Fr.) S. F. Gray, free and total amino acids of the two mushrooms were quantitatively analyzed by G. L. C. and an amino acid analyzer. Free amino acids were extracted from both mushrooms with ethanol. Fourtenn free amino acids were detected from the ethanol extract of S. floccopus and fifteen free amino acids from C. comatus by G. L. C. And the dry carphopores of both mushrooms were hydrolyzed with hydrochloric acid and then the total protein amino acids were analyzed by A. A. A. Seventeen total amino acids were detected from each acid-hydrolysate of S. floccopus and C. comatus. Lipids were extracted from the carpophores of S. floccopus and saponified with alcoholic potassium hydroxide. The isolated sterols were subjected to G. L. C. and two sterols were detected. The isolated free fatty acids were methylated with diazomethane and subjected to column chromatography and G. L. C. Eleven saturated and nine unsaturated free fatty acids were detected from the carpophores of S. floccopus. The presence of these nutrient components shows that the two mushrooms can be utilized as edible ones.

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타우린복용이 정상 성인여성의 혈장 유리아미노산 농도 및 소변내 배설에 미치는 영향 (Effects of Oral Taurine Supplementation on Plasma Concentration and Urinary Excretion of Free Amino Acids in Healthy Female Adults)

  • 차희숙
    • Journal of Nutrition and Health
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    • 제32권2호
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    • pp.158-165
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    • 1999
  • Effects of oral taurine supplementation (6g/day) on plasma concentration and urinary of free amino acids were evaluated in healthy female adults. Among twenty five female volunteers(23.6$\pm$0.3 years old) participated in the taurine supplementation program, twenty four subjects successfully completed the two supplementation program. Plasma and urinary levels of free amino acids were determined by using an automated amino acid analyzer based on ion-exchange chromatography. Two weeks of taurine supplementation resulted in a 65% increase in plasma taurine concentration (p<0.001), Changes in fasting plasma amino acid concentrations followed by taurine supplementation were not spectacular, and were all within the normal range for human aldults. Taurine supplementation significantly elevated urinary methionine, asparagine, hydorxyproline and phosphoserine excretions(31~280%), and significantly decreased the urinary excretions of isoleucine, glutamate and serine compared to the values prior to taurine supplementation. For almost every individual amino acids, 24 hr urinary excretion level was significantly correlated to the urinary excretion value expressed as nmol/mg creatinine(p<0.001). A significant negative correlation found between plasma glutamine concentration and urinary glutamine excretion level suggests that the decrease in plasma glutamine concentration might be associated with the enhanced glutamine excretion in urine followed by taurine supplementation.

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The Analysis of Proximate Composition, Minerals and Amino Acid Content of Red Alga Pyropia dentata by Cultivation Sites

  • Jung, Sang-Mok;Kang, Seul-Gi;Kim, Kwang-Tae;Lee, Han-Joo;Kim, A-Reum;Shin, Hyun-Woung
    • 한국환경생태학회지
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    • 제29권1호
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    • pp.1-6
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    • 2015
  • In this study, the proximate composition of Pyropia dentata was compared by the analysis of minerals and amino acids that were produced at Seocheon, Chungcheongnam-do and at Wando and Jangheung, Jeollanam-do. Moisture, ash and crude proteins were analyzed using the AOAC method, and crude lipid was analyzed using the Soxhlet method, and inorganic analysis was performed using ICP-OES. Amino acid was used for the amino acid analyzer. The proximate composition analysis, moisture content, maximum values were displayed in Wando while the maximum content of ash appeared at Jangheung's Pyropia. Crude proteins was appeared at Seocheon's Pyropia and the maximum amount of crude lipid, carbohydrate was appeared in Wando's Pyropia. Inorganic analysis, calcium, potassium, magnesium, sodium, phosphorus, cobalt, chromium, copper, iron, manganese, nickel, zinc were analyzed from the Seocheon's Pyropia, however, cobalt, chromium, and nickel was not detected in Wando's Pyropia. For amino acid analysis, a total of 17 amino acids were detected: leucine, valine, aspartic acid, glutamic acid, and the amount of alanine accounted for about 57 % of total amino acids(26.1-28.7 %). The proximate composition, minerals, and amino acid of Pyropia dentata were different depending on the coastal ecological habitats.

번식방법(繁殖方法)에 따른 밭고추냉이의 성분비교(成分比較) (Comparison of Chemical Constituents of Upland Wasabia japonica Matsum Grown by Different Propagation Methods)

  • 김상열;박기춘
    • 한국약용작물학회지
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    • 제5권4호
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    • pp.294-301
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    • 1997
  • 향신료(香辛料) 및 고소득 작물로 개발가능성이 있는 고추냉이의 번식방법(繁殖方法)에 따른 성분(成分)을 비교(比較)하여 고추냉이 품질평가 및 우수 품종육성의 기초자료(基礎資料)로 제공(提供)하고자 지폐산(脂肺酸), 아미노산 및 유기산 함양(有機酸 含量) 및 조성(組成)을 조사한 결과(結果)를 (요약)要約하면 다음과 같다. 1. 총지방산 함량 및 지방산조성은 번식방법간에 차이가 없었으나 잎의 지방산함량이 다른 부위보다 높았고, 지방산조성은 부위와 관계없이 linolenic acid 함량이 가장 높았고 그 다음이 Palmitic acid, oleic acid, linoleic acid순이었으며 그 외에 lauric acid, myristic acid, palmitoleic acid, stearic acid, arachidonic acid 소량 함유되어 있었다. 2. 총아미노산 함량은 번식방법 비슷하였으나 부위별로는 잎, 근경, 줄기, 화경순으로 높았고, 아미노산조성은 번식방법에 관계없이 각 부위 모두 glutamic acid $(331{\sim}1568mg)$, asparatic acid $(346{\sim}1380mg)$, arginine $(84{\sim}724mg)$, leucine $(49{\sim}1264mg)$의 함량이 많았다. 3. 유기산 조성은 번식방법에 관계없이 비슷하였며 부위에 관계없이 acetic acid $(60.0{\sim}78.2)$가 가장 함량이 많았고 그 다음은 succinic acid $(9.9{\sim}32.0)$, malic acid $(2.9{\sim}7.9%)$로 함량이 많았다.

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