• 한국의류학회지
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• 제22권7호
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• pp.843-850
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• 1998

The purpose of this study is the investigation of chemical properties of wool treated with oxidants and protease at low temperature. The chemical degradation of the fibers were investigated by measuring $\alpha$-amimo acid contents and FT-IR analysis. In addition, urea-hydrogensulfite solubility was measured to compare to the oxidation and protease treated wool. The results were as follows. 1) By the oxidation of wool, cystine is oxidised to cysteic acid by way of the intermediate oxides, cystine-S-monooxide and cystine-S-dioxide, in the case hydrolysis catalysed by the protease catalyse. Also, $\alpha$-amimo acid contents is increased, and urea-hydrogensulfite solubility was lower than that of untreated wool. This chemical degradation of wool was occurred due to oxidate hydrolysis in the order of permonosulfate>dichloroisocyanuric acid$\geq$chlorine. 2) The chemical degradation of wool was accelerated by the protease treatment of oxidized wool. Oxidation of wool is considered to make the fiber more susceptibled to enzymatic attact by opening disulphide bond within wool. Enzymatic attact was effectively directed to the wool oxidised by permonosulfate.

• BMB Reports
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• 제28권2호
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• pp.87-93
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• 1995

To investigate the biological functions of the EGF-like domain of mouse betacellulin (BTC), mouse BTC(33-80), a 48-residue peptide corresponding to the EGF-like domain, was synthesized by stepwise solidphase methods using a 9-fluorenylmethoxycarbonyl (Fmoc) strategy. The homogeneity of synthetic mouse BTC(33-80) was confirmed by analytical reversed phase (RP)-HPLC, amimo acid analysis, and fast atom bombardment mass spectrometer (FAB-MS). Three disulfide bond pairings of synthetic mouse BTC(33-80) were established by amino acid analysis of cysteine-containing fragments derived from thermolytic digestion. These were consistent with the pairings of EGF and transforming growth factor ($TGF-{\alpha}$). The EGF-Iike domain of mouse BTC showed equipotent activity in both EGF-receptor binding on A-431 epidermoid carcinoma cells, and mitogenesis on NIH-3T3 fibroblast cells, as compared with authentic h-EGF. Results suggest that the EGF-Iike domain of BTC plays a significant role in mitogenic activity with an EGF-receptor mediated system.

• 한국식품영양학회지
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• 제19권1호
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• pp.8-13
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• 2006

본 연구는 남해안에 서식하고 있는 해조류인 지충이에 대하여 기능성 식품으로서의 활용가치를 알아보고자 이화학적 성분을 분석하고 지충이 용매 추출물의 항산화능을 비교하고자 POV와 TBARS를 측정하였다. 지충이의 조단백질과 조지방의 함량은 각각 $15.7{\pm}0.8%$와 $0.9{\pm}0.4%$였다. 9종의 무기질 중 Ca이 $2,288.0{\pm}2.2mg/100g$으로 높게 검출되었고, 아미노산은 glutamic acid가 $1,071.3{\pm}1.8mg/100g$으로 가장 함량이 높았으며, 다음으로 aspartic acid ($645{\pm}1.4mg/100g$) > phenylalanine($470.1{\pm}1.4mg/100g$)의 순이었다. 유리당의 함량은 galactose가 $40.2{\pm}0.5mg/100g$으로 가장 높았고 $FeCl_2$와 $CuSO_4$에 대한 항산화 활성은 추출물의 농도가 높아짐에 따라 항산화능이 증가하는 경향이었고 특히 chloroform 추출물에서 항산화 활성이 우수하였다.