• Environmental Engineering Research
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• 제11권4호
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• pp.217-231
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• 2006

The gas-phase formation of polychlorinated naphthalenes (PCNs) and dibenzofurans (PCDFs) was experimentally investigated by slow combustion of the three chlorophenols (CPs): 2-chlorophenol (2-CP), 3-chlorophenol (3-CP) and 4-chlorophenol (4-CP), in a laminar flow reactor over the range of 550 to $750^{\circ}C$ under oxidative condition. Contrary to the a priori hypothesis, different distributions of PCN isomers were produced from each CP. To explain the distributions of polychlorinated dibenzofuran (PCDF) and PCN congeners, a pathway is proposed that builds on published mechanisms of PCDF formation from chlorinated phenols and naphthalene formation from dihydrofulvalene. This pathway involves phenoxy radical coupling at unsubstituted ortho-carbon sites followed by CO elimination to produce dichloro-9, 10-dihydrofulvalene intermediates. Naphthalene products are formed by loss of H and/or Cl atoms and rearrangement. The degree of chlorination of naphthalene and dibenzofuran products decreased as temperature increased, and, on average, the naphthalene congeners were less chlorinated than the dibenzofuran congeners. PCDF isomers were found to be weakly dependent to temperature, suggesting that phenoxy radical coupling is a low activation energy process. Different PCN isomers, on the other hand, are formed by alternative fusion routes from the same phenoxy radical coupling intermediate. PCN isomer distributions were found to be more temperature sensitive, with selectivity to particular isomers decreasing with increasing temperature.

• Journal of Microbiology and Biotechnology
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• 제30권11호
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• pp.1729-1738
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• 2020

Salmonellosis is a form of gastroenteritis caused by Salmonella infection. The main transmission route of salmonellosis has been identified as poorly cooked meat and poultry products contaminated with Salmonella. However, in recent years, the number of outbreaks attributed to contaminated raw produce has increased dramatically. To understand how Salmonella adapts to produce, transcriptomic analysis was conducted on Salmonella enterica serovar Virchow exposed to fresh-cut radish greens. Considering the different Salmonella lifestyles in contact with fresh produce, such as motile and sessile lifestyles, total RNA was extracted from planktonic and epiphytic cells separately. Transcriptomic analysis of S. Virchow cells revealed different transcription profiles between lifestyles. During bacterial adaptation to fresh-cut radish greens, planktonic cells were likely to shift toward anaerobic metabolism, exploiting nitrate as an electron acceptor of anaerobic respiration, and utilizing cobalamin as a cofactor for coupled metabolic pathways. Meanwhile, Salmonella cells adhering to plant surfaces showed coordinated upregulation in genes associated with translation and ribosomal biogenesis, indicating dramatic cellular reprogramming in response to environmental changes. In accordance with the extensive translational response, epiphytic cells showed an increase in the transcription of genes that are important for bacterial motility, nucleotide transporter/metabolism, cell envelope biogenesis, and defense mechanisms. Intriguingly, Salmonella pathogenicity island (SPI)-1 and SPI-2 displayed up- and downregulation, respectively, regardless of lifestyles in contact with the radish greens, suggesting altered Salmonella virulence during adaptation to plant environments. This study provides molecular insights into Salmonella adaptation to plants as an alternative environmental reservoir.

• 산업경영시스템학회지
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• 제43권3호
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• pp.77-86
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• 2020

In this study, to improve the logistics flow of existing given chemical logistics warehouse, four logistics flow alternatives were proposed to minimize truck interference by building simulation model. The simulation model for chemical storage warehouse was built to evaluate system performance. Among the four new improved alternatives based on the basic model, the model with the same truck's pathways and locations of facilities identified an increase in the number of interferences but a decrease in daily working hours as the number of resources in a particular facility increases. Therefore, the three groups were classified as 'efficiency', 'complementary', and 'safety' based on the daily working hours, and the ratio of trucks entering two types of logistics warehouse was set in consideration of future market fluctuations. For each of the six types, the optimal number of resources was selected as the number of resources in the facilities with the least number of interferences in the basic model and the evaluation measures and characteristics set in this study were compared and analyzed. As a result, the Alternative 4 model operating the underground roadway produced interference between 17.0% and 36.4% of the basic model, with 113.3% of the interior loadspace.

• BMB Reports
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• 제53권6호
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• pp.291-298
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• 2020

Tumor angiogenesis is an essential process for growth and metastasis of cancer cells as it supplies tumors with oxygen and nutrients. During tumor angiogenesis, many pro-angiogenic factors are secreted by tumor cells to induce their own vascularization via activation of pre-existing host endothelium. However, accumulating evidence suggests that vasculogenic mimicry (VM) is a key alternative mechanism for tumor vascularization when tumors are faced with insufficient supply of oxygen and nutrients. VM is a tumor vascularization mechanism in which tumors create a blood supply system, in contrast to tumor angiogenesis mechanisms that depend on pre-existing host endothelium. VM is closely associated with tumor progression and poor prognosis in many cancers. Therefore, inhibition of VM may be a promising therapeutic strategy and may overcome the limitations of anti-angiogenesis therapy for cancer patients. In this review, we provide an overview of the current anti-angiogenic therapies for ovarian cancer and the current state of knowledge regarding the links between microRNAs and the VM process, with a focus on the mechanism that regulates associated signaling pathways in ovarian cancer. Moreover, we discuss the potential for VM as a therapeutic strategy against ovarian cancer.

• 한국산학기술학회:학술대회논문집
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• 한국산학기술학회 2004년도 춘계학술대회
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• pp.253-257
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• 2004

Platelet aggregation is a complex phenomenon that probably involves several intracellular biochemical pathways. When activated, platelets change shape, aggregate and release the contents of their intracellular granules. The interactions between platelets and blood vessel walls are important in the development of thrombosis and cardiovascular diseases. When blood vessels are damaged, platelet aggregation occurs rapidly to form haemostatic Plugs or arterial thrombi at the sites of vessel injury or in regions where blood flow is disturbed. These thrombi are the source of thromboembolic complications of atherosclerosis, heart attacks, stroke, and peripheral vascular disease. Therefore, the inhibition of platelet function represents a promising approach for the prevention of thrombosis. Plants constitute a rich source of bioactive chemicals such as phenolics, terpenoids and alkaloids. Plant extracts may be an alternative to currently used medicinal source because they constitute a rich source of bioactive chemicals. This study was performed to investigate the antiplatelet activity of extract of Tabebuia impetiginosa Martius ex DC (Taheebo) and find out which fractions to this activity in rabbit platelet. Taheebo was methanol extracted and solvent fractionated in to five fractions (hexane, chloroform, ethylacetate, butanol and water). And each fractions were investigated inhibitory effects on platelet aggregation induced by various agonists using washed rabbit platelets in vitro.

• Experimental and Molecular Medicine
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• 제50권11호
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• pp.12.1-12.12
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• 2018

Drugs targeting the epidermal growth factor receptor (EGFR), such as cetuximab and panitumumab, have been prescribed for metastatic colorectal cancer (CRC), but patients harboring KRAS mutations are insensitive to them and do not have an alternative drug to overcome the problem. The levels of ${\beta}$-catenin, EGFR, and RAS, especially mutant KRAS, are increased in CRC patient tissues due to mutations of adenomatous polyposis coli (APC), which occur in 90% of human CRCs. The increases in these proteins by APC loss synergistically promote tumorigenesis. Therefore, we tested KYA1797K, a recently identified small molecule that degrades both ${\beta}$-catenin and Ras via $GSK3{\beta}$ activation, and its capability to suppress the cetuximab resistance of KRAS-mutated CRC cells. KYA1797K suppressed the growth of tumor xenografts induced by CRC cells as well as tumor organoids derived from CRC patients having both APC and KRAS mutations. Lowering the levels of both ${\beta}$-catenin and RAS as well as EGFR via targeting the $Wnt/{\beta}$-catenin pathway is a therapeutic strategy for controlling CRC and other types of cancer with aberrantly activated the $Wnt/{\beta}$-catenin and EGFR-RAS pathways, including those with resistance to EGFR-targeting drugs attributed to KRAS mutations.

• Korean Chemical Engineering Research
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• 제57권1호
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• pp.85-89
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• 2019

무세포 단백질 합성 시스템은 세포를 파쇄한 후 파쇄액 내의 단백질 합성기구들을 이용하여 단백질을 발현하는 시스템으로 기존의 세포 기반 재조합 단백질 발현 기법들과 달리 세포의 생장조건에 영향을 받지 않으면서 발현 조절에 관한 다양한 인자들을 인위적으로 조절 할 수 있는 장점이 있다. 그러나, 단백질 합성 과정 중 소모되는 ATP의 연속적 재생을 위해 사용되는 에너지원의 높은 비용과 낮은 안정성은 재조합 단백질 대량생산에의 적용을 제약하는 요인으로 작용하여 왔다. 이러한 문제를 해결하기 위한 대안들 중의 하나로 포도당을 에너지원으로 사용하여 세포 파쇄액내 대사과정을 통해 ATP를 재생하는 방법이 있다. 본 연구에서는 포도당을 에너지원으로 이용한 무세포 합성 시스템에서의 단백질 합성 효율 향상을 위하여 대장균 파쇄액으로부터 회수된 지질을 추가적으로 첨가함으로써 산화적 인산화 과정에서의 ATP재생을 증진시키고자 하였다. 그 결과, 지질이 추가된 무세포 단백질 합성 시스템은 지질이 추가되지 않은 대조군에 비하여 6배 이상 향상된 단백질 생산성을 나타내었다.

• Molecules and Cells
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• 제42권3호
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• pp.237-244
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• 2019

Understanding the mechanisms of cancer drug resistance is a critical challenge in cancer therapy. For many cancer drugs, various resistance mechanisms have been identified such as target alteration, alternative signaling pathways, epithelial-mesenchymal transition, and epigenetic modulation. Resistance may arise via multiple mechanisms even for a single drug, making it necessary to investigate multiple independent models for comprehensive understanding and therapeutic application. In particular, we hypothesize that different resistance processes result in distinct gene expression changes. Here, we present a web-based database, CDRgator (Cancer Drug Resistance navigator) for comparative analysis of gene expression signatures of cancer drug resistance. Resistance signatures were extracted from two different types of datasets. First, resistance signatures were extracted from transcriptomic profiles of cancer cells or patient samples and their resistance-induced counterparts for >30 cancer drugs. Second, drug resistance group signatures were also extracted from two large-scale drug sensitivity datasets representing ~1,000 cancer cell lines. All the datasets are available for download, and are conveniently accessible based on drug class and cancer type, along with analytic features such as clustering analysis, multidimensional scaling, and pathway analysis. CDRgator allows meta-analysis of independent resistance models for more comprehensive understanding of drug-resistance mechanisms that is difficult to accomplish with individual datasets alone (database URL: http://cdrgator.ewha.ac.kr).

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2016년도 학술발표회
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• pp.594-598
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• 2016

기후변화는 사회 경제 환경부분에 영향을 미치며 수자원 부문에서는 안정적인 물 공급에 위기가 발생할 것으로 전망되고 있다. 인간이 이용하는 수자원은 강우에 의해 가장 민감하게 반응하므로 지속적이고 안정적인 수자원을 이용하기 위해서는 통합적이고 탄력적인 대응방안이 요구되고 있다. 특히 우리나라와 같은 여름에 집중적 강우가 나타나는 지역에서의 용수수급에 대한 중요성은 더욱더 커질 것이다. 또한 앞으로 1인당 물 수요량의 증가할 것으로 예상됨에 따라 용수수급 관리를 위한 적응전략 수립은 반드시 필요할 것이다. 본 연구에서는 수문모형인 SWAT을 이용하여 자연유량을 산출하기 위해 SWAT-CUP을 이용한 검보정을 수행하여 모형의 적용성을 검토하였다. 기후변화 시나리오인 RCP시나리오를 이용하여 기간별 자연유량을 산정하였다. 유역의 생활용수, 공업용수, 농업용수 및 하천유지유량 데이터를 수집하여 물 분배 모형인 K-MODSIM 모형에 적용하였다. 2011년부터 2100년까지 월별로 유역의 물 수급의 과부족을 산정하였다. 용수의 부족이 나타난 지역에 대한 수요관리와 공급관리 방안을 수립하였고 대안을 적용한 후 용수 공급에 미치는 영향을 다시 분석하였다. 수문모의 결과 RCP 시나리오별로 자연유량을 산정한 결과 시간의 흐름에 따라 물 공급량이 감소할 것으로 분석되었고 계절별로 차이는 더 크게 나타났다. 특히 갈수기인 봄과 겨울철의 유량감소가 나타났다. 모의된 월별 자연유량을 K-MODSIM에 입력하여 월별 모의를 수행한 결과 각 용수별로 부족현상이 나타났다. 생활용수의 부족이 나타난 경우 수요관리를 통해 물 수요량을 줄이는 적응전략을 수립하였고 부족량이 크게 나타나는 농업용수와 하천유지유량의 경우 공급량을 늘리는 공급관리를 통한 적응전략을 수립하여 효과분석을 수행하였다. 단계별로 용수공급에 대한 효과가 나타났다. 하지만 본 연구에서는 경제성 분석을 제외한 용수공급에 중점을 둔 적응전략을 수립하였으므로 이를 포함한 분석이 포함된다면 실제 용수공급 대안 선정을 위한 의사결정에 도움이 될 수 있을 것이다.

• 문화기술의 융합
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• 제10권3호
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• pp.559-564
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• 2024

산업혁명 이래 쓰레기 배출은 증가해 왔으며 폐기물의 처리 문제는 오늘날 인류가 직면한 가장 대표적 환경문제 중 하나가 되었다. 그러나 쓰레기는 님비 운동을 촉발하는 등, 처리 문제를 둘러싸고 다양한 국가, 정부 및 사회 주체들 간의 갈등은 고조되고 있다. 국가와 지방정부 수준에서 쓰레기 문제의 처리 방식으로는 제도, 법, 규제, 시장적 접근법, 합의와 협력적 거버넌스 등 다양한 방안이 도입, 적용되어 왔다. 최근에는 순환 경제라는 개념 하에 과학 기술을 적용하여 폐기물을 재활용, 재자원화하는 등의 방안도 주목받고 있다. 그러나 쓰레기 문제 해결을 위한 사회적 지지는 여전히 불충분하며 이에 새로운 접근법과 대안 모색이 필요한 시점이다. 본 논문은 훈데르트바서라는 예술가가 설계한 오스트리아 수도 빈의 슈피텔라우 소각장과 일본 오사카의 마이시마 소각장을 사례연구(case study) 하였다. 이들 사례는 오스트리아 출신의 훈데르트바서가 예술과 기술의 창의적, 혁신적 융합을 통해 어떻게 폐기물과 같은 도시 환경문제이자 공공의 문제에 대한 새로운 대안과 접근법을 제시하였는지 보여준다. 이들 사례는 오늘날의 환경문제 해결에 있어 과학기술적. 경제적 접근법과 더불어 예술과 문화의 융합이 유용할 수 있음을 시사한다.