• 한국수정란이식학회지
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• 제25권4호
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• pp.259-262
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• 2010

Optimization of the preimplantation mammalian embryo culture condition was widely focused on refining medium composition under the name of chemically defined media. However, recent research revealed that the alteration of physical environment can be a crucial factor to a successful embryo development. In this study, under the same embryo density, a novel culture device named oil-free micro tube culture (MTC) system was evaluated using porcine parthenogenetic embryos. The activated oocytes were placed into the 0.2 ml thin-wall flat cap PCR tube and cultured to the blastocyst stage. As a preliminary step, embryo density and culture medium volume were optimized under a standard drop culture system. The optimal embryo density range for in vitro culture was 0.5 embryos per ${\mu}l$ in $20\;{\mu}l$ drop (20.5%) and 1.0 embryos per ${\mu}l$ in $10\;{\mu}l$ drop (20.6%). Based on these results, we compared drop culture system and 'MTC' system in terms of the developmental rate to the blastocyst stage. In $20\;{\mu}l$ medium volume, the 'MTC' system showed similar blastocyst formation rate when compared with drop culture system (20.2% versus 20.5%, respectively) while the 'MTC' system showed lower blastocyst formation rate than drop culture system in $10\;{\mu}l$ one (12.7% versus 20.0%, respectively). Therefore the $20\;{\mu}l$ MTC system may be an alternative incubation system for short-distance embryo transport without carrying the $CO_2$ incubator and this provides novel embryo culture device to clinical veterinary embryologists.

• 한국게임학회 논문지
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• 제17권2호
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• pp.123-134
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• 2017

본 연구에서는 학교 장면에서 학생들의 디지털 게임 이용에 대한 교사의 수용도가 어떻게 구성되어 있는지를 살펴보고, 교사의 수용도에 영향을 미치는 요인에 대해 알아보았다. 이를 위해 2016년 8월 설문조사를 실시하였고, 초, 중, 고등학교 교사와 상담교사, 전문 상담사, 총 250명의 자료가 분석되었다. 그 결과, 학생들의 디지털 게임 이용에 대한 교사의 수용도는 5개의 하위요인인 관리/감독의 필요성, 적극 활용의사, 관심과 확인, 부적응 대체활동으로 인정, 수용 효능감으로 구성되어있었다. 다변량다중회귀분석방법을 통해 분석한 결과, 교사의 연령, 디지털 미디어 리터러시의 하위요인 중 공동체성과 탈규범성, 학생들의 디지털 게임 이용에 대한 교사 태도와 디지털 게임이 학업에 미치는 영향에 대한 교사 평가가 교사의 수용도 5개 하위요인에 차별적으로 영향을 미치는 것으로 확인되었다. 본 연구는 교육적 잠재력이 강조되고 있는 디지털 게임에 대한 교사의 수용도가 어떻게 구성되어있는지를 구체적으로 확인하고, 교사의 수용도를 촉진하는데 영향을 주는 주요 요인들을 밝혔다는데 그 의의가 있다.

• Mycobiology
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• 제42권4호
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• pp.346-352
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• 2014

Recently, the Q biotype of tobacco whitefly has been recognized as the most hazardous strain of Bemisia tabaci worldwide, because of its increased resistance to some insecticide groups. As an alternative control agent, we selected an Isaria javanica isolate as a candidate for the development of a mycopesticide against the Q biotype of sweet potato whitefly. To select optimal mass production media for solid-state fermentation, we compared the production yield and virulence of conidia between 2 substrates (barley and brown rice), and we also compared the effects of various additives on conidia production and virulence. Barley was a better substrate for conidia production, producing $3.43{\times}10^{10}$ conidia/g, compared with $3.05{\times}10^{10}$ conidia/g for brown rice. The addition of 2% $CaCO_3+2%$ $CaSO_4$ to barley significantly increased conidia production. Addition of yeast extract, casein, or gluten also improved conidia production on barley. Gluten addition (3% and 1.32%) to brown rice improved conidia production by 14 and 6 times, respectively, relative to brown rice without additives. Conidia cultivated on barley produced a mortality rate of 62% in the sweet potato whitefly after 4-day treatment, compared with 53% for conidia cultivated on brown rice. The amendment of solid substrate cultivation with additives changed the virulence of the conidia produced; the median lethal time ($LT_{50}$) was shorter for conidia produced on barley and brown rice with added yeast extract (1.32% and 3%, respectively), $KNO_3$ (0.6% and 1%), or gluten (1.32% and 3%) compared with conidia produced on substrates without additives.

• Journal of Microbiology
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• 제44권3호
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• pp.293-300
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• 2006

The Escherichia coli heat-labile enterotoxin B subunit (HLT-B) is one of the most powerful mucosal immunogens and known mucosal adjuvants. However, the induction of high levels of HLT-B expression in E. coli has proven a difficult proposition. Therefore, in this study, the HLT-B gene was cloned from pathogenic E. coli and expressed as a fusion protein with GST (glutathion S-transferase) in E. coli BL2l (DE3), in an attempt to harvest a large quantity of soluble HLT-B. The culture conditions, including the culture media used, temperature, pH and the presence of lactose as an inducer, were all optimized in order to obtain an increase in the expression of soluble GST-rHLT-B. The biological activity of the purified rHLT-B was assayed in a series of GMI-ELISA experiments. The findings of these trials indicated that the yield of soluble recombinant GST-rHLT-B could be increased by up to 3-fold, as compared with that seen prior to the optimization, and that lactose was a more efficient alternative inducer than IPTG. The production of rHLT-B, at 92 % purity, reached an optimal level of 96 mg/l in a 3.7 L fermentor. The specific GM1 binding ability of the purified rHLT-B was determined to be almost identical to that of standard CTB.

• Journal of Microbiology and Biotechnology
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• 제27권2호
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• pp.342-349
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• 2017

Polylactic acid (PLA) has been highlighted as an alternative renewable polymer for the replacement of petroleum-based plastic materials, and is considered to be biodegradable. On the other hand, the biodegradation of PLA by terminal degraders, such as microorganisms, requires a lengthy period in the natural environment, and its mechanism is not completely understood. PLA biodegradation studies have been conducted using mainly undefined mixed cultures, but only a few bacterial strains have been isolated and examined. For further characterization of PLA biodegradation, in this study, the PLA-degrading bacteria from digester sludge were isolated and identified using a polymer film-based screening method. The enrichment of sludge on PLA granules was conducted with the serial transference of a subculture into fresh media for 40 days, and the attached biofilm was inoculated on a PLA film on an agar plate. 3D optical microscopy showed that the isolates physically degraded the PLA film due to bacterial degradation. 16S rRNA gene sequencing identified the microbial colonies to be Pseudomonas sp. MYK1 and Bacillus sp. MYK2. The two isolates exhibited significantly higher specific gas production rates from PLA biodegradation compared with that of the initial sludge inoculum.

• 문화기술의 융합
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• 제6권2호
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• pp.349-355
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• 2020

현대 사회의 사회적 문제를 위한 대안으로 소셜로봇이 주목받고 있으며 소셜로봇의 효율적 관리를 위해 모듈화의 필요성이 제기되었다. 본 연구는 '개인용 소셜 서비스 로봇'의 개념을 재정립하고 이에 적용하기 위한 새로운 모듈화 방식을 제안하는 것을 목적으로 한다. 이를 위해 소셜 로봇과 서비스 로봇의 정의와 로봇의 모듈화에 대한 문헌연구를 수행하였다. 기존 서비스 로봇의 모듈화 사례를 분석하여 소셜 서비스 로봇의 모듈화에 적용하기 위한 고려사항을 도출하고 이를 바탕으로 새로운 모듈화 방안을 제안하였다. 본 연구는 모듈의 전기/전자적 부품 여부에 따라 액티브 모듈과 패시브 모듈로 구분하고, 액티브 모듈은 다시 로봇의 기본형과 교체형에 따라 기본 모듈, 추가 모듈로 분류했다. 이러한 방안이 적용된 실제 로봇의 프로토타입을 제작하는 것으로 모듈화를 검증하였다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제37권4호
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• pp.278-286
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• 2011

Introduction: This study examined the predictability of new bone formation in the pneumatized maxillary sinus using only fibrin-rich blocks with concentrated growth factors as an alternative to bone grafts. Materials and Methods: Maxillary sinus augmentation was performed in thirty-three patients with a deficient alveolar bone height (mean 3.9 mm). All patients were treated consecutively with sinus membrane elevation via the lateral window approach and panoramic radiograms and cone-beam computed tomograms were taken to evaluate the remaining bone height and the new bone formation in the maxillary sinus, before and after surgery. Four biopsy specimens were taken at the time of implant consolidation (after an average of five months healing) and were stained by H & E and Trichrome staining. Results: None of the patients had postoperative complications during implant consolidation. After an average of 5 months since sinus augmentation, newly formed bone was observed in all cases by a radiographic evaluation. In 4 biopsy samples, newly formed bone was observed along the floor of the replaced bony window. The osteoblast lining and well distinguished Osteocytes in the lacunas were observed in the newly formed bone. Of the 74 implants (4 different surfaced implants - resorbable blast media-surfaced (RBM), Hydroxyapatite (HA) coated, acid-etched, sintered porous-surfaced implant) placed, one RBM implant failed. The success rate was 98.6% after a mean of 15 months. Discussion: These results suggest that maxillary sinus augmentation using fibrin rich block with concentrated growth factors is a successful and predictable technique.

• 한국실내디자인학회논문집
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• 제15권6호
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• pp.77-88
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• 2006

According to critics and architects, non-linear structure is not only an organic form of space, but also a form of space detached from modem style. Accordingly, non-linear structure can be accepted as an alternative to what has remained unsolved by deconstructionist. However, they are criticized for not clarifying the interdependent relationship between non-linearity of space and cognitive structure of human being. They ended up remaining the hypothesis just an intuitive and abstract one. This research began on the basis that their hypothesis is hard to be objectified, and it needs further inquiry. The purpose of this thesis is to explore how the redundant factors constitute non-linear structures of digital media centered space design. Geometric compositions of space structure were analyzed to define what types of redundant factors are contrived in the process of visual information. This study about the visual form, researching the Information Theory, and then offer a quantitative analysis that makes those more objective. Space structure and geometric composition were analyzed to define what types of redundancy are contrived in the process of visual information. In particular, I put higher theoretical emphasis on what characteristics are ensued in the process of structuring spaces than any other subjects. Followings are the conclusion of analysis. First, as a result of examining, we can assume that NOX' space structure is not a chaotic form, but has an operating the form of its own. Second, in case of curvilinear, the structure was found redundancy on mid deviation ratio and discontinuous circular fabric. Although most of their structures appeared complex with a higher coherent constant, they were found to be stable factors because of the low deviation ratio between systems. The amount of surplus information was stable structure as well.

• KIEAE Journal
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• 제10권6호
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• pp.73-80
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• 2010

Since the Korean War, Korea has experienced modernization. The population increase by baby booming has asked for more space for educational facilities. In such a situation, the purpose of educational facilities was to accommodate continuously increasing students, rather than seeking for quantitative demands. In addition, in accordance with social changes, educational shifts were required. After the revision of the seventh national curriculum in education in 1997, the school buildings became varied. The design of buildings in accordance with educational curriculum has been improved, but still lack of forming comfortable environment and considering energy efficiency in school buildings. For the improvement of educational environments, educational media such as TV and computers have been provided, and energy systems, including heating and cooling systems, has been continuously increased. As a result, it appeared that energy use in school buildings and facilities has been steadily increased and that the structure of energy consumption has been also changed, especially with regard to electricity use. Living in the 21st century, human beings face global environmental issues, such as global warming, geographical climate changes, and ozone destruction that are the consequences of fossil energy use. Therefore, even in industrial areas, considering a counterplan for low energy use is being paid attention. Starting with Kyoto Protocol in 1992, people try to decrease carbon dioxide and to develop alternative energies (i.e. natural energy); for example, solar energy, wind force, terrestrial heat, and water power. Advanced countries already set up a criterion for $CO_2$ decrease ranging from office buildings to residential houses and also propose alternatives for the $CO_2$ decrease. However, there is no such a plan for low energy use and $CO_2$ decrease in school facilities, and any research on the actual conditions was not accomplished. Thus, this study examines energy demand in classrooms that take up a large portion of energy demand in school building structure.

• 한국초전도ㆍ저온공학회논문지
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• 제16권1호
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• pp.36-41
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• 2014

Since glass microsphere has high crush strength, low density and small particle size, it becomes alternative thermal insulation material for cryogenic systems, such as storage and transportation tank for cryogenic fluids. Although many experiments have been performed to verify the effective thermal conductivity of microsphere, prediction by calculation is still inaccurate due to the complicated geometries, including wide range of powder diameter distribution and different pore sizes. The accurate effective thermal conductivity model for microsphere is discussed in this paper. There are four mechanisms which contribute to the heat transfer of the evacuated powder: gaseous conduction ($k_g$), solid conduction ($k_s$), radiation ($k_r$) and thermal contact ($k_c$). Among these components, $k_g$ and $k_s$ were calculated by Zehner and Schlunder model (1970). Other component values for $k_c$ and $k_r$, which were obtained from experimental data under high vacuum conditions were added. In this research paper, the geometry of microsphere was simplified as a homogeneous solid sphere. The calculation results were compared with previous experimental data by R. Wawryk (1988), H. S. Kim (2010) and the experiment of this paper to show good agreement within error of 46%, 4.6% and 17 % for each result.