• 한국미생물·생명공학회지
• /
• 제24권6호
• /
• pp.717-725
• /
• 1996

We have developed an efficient immobilized cell separator for continuous operation of immobilized fungal cell cultures, and applied this separator to actual fermentation process for the production of cyclosporin A (CyA), a powerful immunosuppressant. In the experiments employing highly viscous polymer (carboxymethyl cellulose) solution, the decantor showed good separating performances at high solution viscosites and fast dilution rates. Air duct and cylindrical separator installed inside the decantor turned out to play key roles for the efficient separation of the immobilized cells. By installing the decantor in an immobilized perfusion reactor system (IPRS), continuous immobilized culture was stably carried out even at high dilution rate for a long period, leading to high productivities of free cells and CyA. Almost no immobilized biomass existed in effuluent stream of the IPRS, demonstrating the effectiveness of the decan- tor system for a long-term continuous fermentation. It was noteworthy that we could obtain these results despite of the unfavorable fermentation conditions, i.e., reduced density of the biosupports caused by overgrowth of cells inside the bead particles and existence of high density of suspended fungal cells (10g/l) in the fermentation broth.

• 한국물환경학회지
• /
• 제25권1호
• /
• pp.125-135
• /
• 2009

Reliable long-term flows by SWAT-K model were applied to the relationship between stream flow and pollutant load derived from 8-day measured data of Ministry of Environment (MOE) in order to obtain continuous loadograph and evaluate accuracy in water quality modeling for the Chungju dam watershed. The measured flow were compared with flow duration curve from the model, and it showed that measured values corresponded to the almost full range of stream flow conditions except at Odae A. And there was significant relationship ($R^2=0.60{\sim}0.97$) between measured flow and water quality load at all unit-watersheds. Applying this relationship to simulated flows, continuous loadograph was obtained and compared with modeled pollutant loads. Although there were some differences during some dry and flood seasons, those were not significant and overall trend showed a good agreement. From the results, we would be able to derive a continuous loadograph based on measured data at total maximum daily loads (TMDLs) unit-watersheds on a national scale, in which stream flow and water quality have been measured at 8-day intervals since 2004, and this could be helpful to utilize distributed water quality models with difficulty in calibrating and validating parameters from lack of measured data at present.

• Nuclear Engineering and Technology
• /
• 제52권7호
• /
• pp.1532-1536
• /
• 2020

For ensuring nuclear safeguards, we report the analytical signal-detection performance of thermal ionization mass spectrometry (TIMS) with continuous heating for the measurement of isotopic ratios in samples containing ultra-trace amounts of uranium. As methods for detecting uranium signals, peak-jumping mode using a single detector and static mode using multiple detectors were examined with U100 (10% ²³⁵U-enriched) uranium standard samples in the femtogram-to-picogram range. Uranium isotope ratios, n(²³⁵U)/n(²³⁸U), were measured down to levels of 1 fg and 3 fg in static and peak-jumping modes, respectively, while n(²³⁴U)/n(²³⁸U) and n(²³⁶U)/n(²³⁸U) values were measured down to levels of 100 fg in both modes. In addition, the dependency of the ²³⁸U signal intensity on sample quantity exhibited similar tendencies in both modes. The precisions of the isotope ratios obtained in the static mode over all sample ranges used in this study were overall slightly higher than those obtained in peak-jumping mode. These results indicate that isotope ratio measurements by TIMS with continuous heating are almost independent of the detection method, i.e., peak-jumping mode or static mode, which is characteristic of isotope-ratio measurements using the TIMS method with continuous heating. TIMS with continuous heating is advantageous as it exhibits the properties of multiple detectors within a single detector, and is expected to be used in various fields in addition to ensuring nuclear safeguards.

• Tribology and Lubricants
• /
• 제9권2호
• /
• pp.22-28
• /
• 1993

The experimental investigation on the inlet pressure of a large tilting pad journal bearing is studied. The continuous distribution of the film pressure and film thickness are measured along with the shaft speed and bearing load for various flow rates. Considerably large inlet pressure is observed at the entrance of each pad, especially lower pads. The inlet pressure increases with the increase of shaft speed as well as bearing load, but it is almost independent on the folw rate and the oil supply temperature. Because of the inlet pressure, the upper pads always keep up slight wedge film shape which are almost parallel to the journal surface, and spragging is not observed in the upper pads with no preload.

• 대한수학회지
• /
• 제41권3호
• /
• pp.461-477
• /
• 2004

It is shown that every almost linear mapping h : A\longrightarrowB of a unital $C^{*}$ -algebra A to a unital $C^{*}$ -algebra B is a homomorphism under some condition on multiplication, and that every almost linear continuous mapping h : A\longrightarrowB of a unital $C^{*}$ -algebra A of real rank zero to a unital $C^{*}$ -algebra B is a homomorphism under some condition on multiplication. Furthermore, we are going to prove the generalized Hyers-Ulam-Rassias stability of *-homomorphisms between unital $C^{*}$ -algebras, and of C-linear *-derivations on unital $C^{*}$ -algebras./ -algebras.

• Journal of Photoscience
• /
• 제4권3호
• /
• pp.141-145
• /
• 1997

Polymerase chain reaction(PCR) was conducted with a pea cDNA library using two primers synthesized from homology analysis of amino acid sequences for animal and plant cytosolic FBPases. A PCR product with 650 bp long was cloned into pGEM-T vector and sequenced. The deduced amino acid sequence of the cDNA fragment was 98, 91, and 85% homologous with those of cytosolic FBPases from spinach, sugarbeet, and sugarcane, respectively. It was 51% homologous with amino acid sequence of FBPase from pea chloroplasts. Northern blot analysis was proceeded with the cDNA clone resulting that 1.2 kb transcript was highly expressed in light-grown pea leaves but almost not expressed in dark-grown etiolated pea seedlings. When peas grown in the light for 10 days were transferred to darkness, the transcript was gradually decreased with dark treatment, indicating that the expression of the enzyme was induced by continuous white light but suppressed by dark treatment. Pea cytosolic FBPase was highly expressed in leaves with trace amounts in stems. but almost not expressed in roots.

• 대한수학회보
• /
• 제31권2호
• /
• pp.187-192
• /
• 1994

The author extended the small properties of topological semilattices to that of regular semigroups [3]. In this paper, it could be shown that a semigroup S is almost regular if and only if over bar RL = over bar R.cap.L for every right ideal R and every left ideal L of S. Moreover, it has shown that the Bohr compactification of an almost regular semigroup is regular. Throughout, a semigroup will mean a topological semigroup which is a Hausdorff space together with a continuous associative multiplication. For a semigroup S, we denote E(S) by the set of all idempotents of S. An element x of a semigroup S is called regular if and only if x .mem. xSx. A semigroup S is termed regular if every element of S is regular. If x .mem. S is regular, then there exists an element y .mem S such that x xyx and y = yxy (y is called an inverse of x) If y is an inverse of x, then xy and yx are both idempotents but are not always equal. A semigroup S is termed recurrent( or almost pointwise periodic) at x .mem. S if and only if for any open set U about x, there is an integer p > 1 such that x$^{p}$ .mem.U.S is said to be recurrent (or almost periodic) if and only if S is recurrent at every x .mem. S. It is known that if x .mem. S is recurrent and .GAMMA.(x)=over bar {x,x$^{2}$,..,} is compact, then .GAMMA.(x) is a subgroup of S and hence x is a regular element of S.

• Biotechnology and Bioprocess Engineering:BBE
• /
• 제9권6호
• /
• pp.454-458
• /
• 2004

A study of the POSE (predispersed solvent extraction) for succinic acid by colloidalliq­uid aphrons was conducted. The organic phase contaning TOA (tri-n-octylamine) and 1-octanol permits a selective extraction of succinic acid from its aqueous solution. There was no difference of the extractability of POSE and that of conventional mixer-settler type extraction. Taking into account the no mechanical mixing in POSE, it was concluded that the POSE process is more adaptive than the conventional mixer-settler type extraction process. From mass transfer analysis at the various concentration of TOA in counter-current continuous operation, the concentration of TOA had no influence on the mass transfer coefficient. The loading values in continuous POSE were almost same as those in batch operation.

• 대한수학회지
• /
• 제38권6호
• /
• pp.1191-1204
• /
• 2001

The paper is devoted to the study of fractional integration and differentiation on a finite interval [a, b] of the real axis in the frame of Hadamard setting. The constructions under consideration generalize the modified integration $\int_{a}^{x}(t/x)^{\mu}f(t)dt/t$ and the modified differentiation ${\delta}+{\mu}({\delta}=xD,D=d/dx)$ with real $\mu$, being taken n times. Conditions are given for such a Hadamard-type fractional integration operator to be bounded in the space $X^{p}_{c}$(a, b) of Lebesgue measurable functions f on $R_{+}=(0,{\infty})$ such that for c${\in}R=(-{\infty}{\infty})$, in particular in the space $L^{p}(0,{\infty})\;(1{\le}{\le}{\infty})$. The existence almost every where is established for the coorresponding Hadamard-type fractional derivative for a function g(x) such that $x^{p}$g(x) have $\delta$ derivatives up to order n-1 on [a, b] and ${\delta}^{n-1}[x^{\mu}$g(x)] is absolutely continuous on [a, b]. Semigroup and reciprocal properties for the above operators are proved.

• 한국환경보건학회지
• /
• 제23권1호
• /
• pp.74-80
• /
• 1997

This study was performed to estimate the ozonation characteristics of phenol wastewater with increasing pH in the continuous packed column reactor (PCR) and the bubble column reactor (BCR). Among various influencing factors that affect phenol on decomposition through the ozonation, pH was chosen as reaction parameter. Upon increasing pH from 3 to 9, the phenol removal efficiency in PCR was improved approximately by 17% while in BCR approximately by 19.2%. The improvements in the phenol removal efficiency by increasing pH caused the enhancements in ozone utilization efficiency reaching almost 100% in PCR at pH 9. In conclusions, ozone has latent power for phenol wastewater treatment, and the performance of PCR was superior to that of BCR in the aspects of phenol removal and ozone utilization efficiency.