• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.3
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• pp.281-285
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• 1997

Essential oil extracted by solvent, n-pantane and diethyl ether for 2 hours was 0.1∼0.5％ in Wasabia japonica Mastum. and it was showed the highest content in root and the lowest content in petiole. Allylisothiocyanate detected by gas chromatography contained in all of the plant part in wasabi. Its content in rhizome was 0.687∼1.339mg /g FW showing the highest content in rhizome. Allylisothiocyanate content was varied from culture site, variety and position of rhizome in wasabi.

• Journal of the East Asian Society of Dietary Life
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• v.6 no.1
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• pp.51-58
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• 1996

The effects of allylisothiocyanate on the biosynthesis of various fungus metabolites such as sterigmatocystin, lipid, protein, citrate RNA and AMP from the culture of Aspergillus Parasiticus R-716 were investigated. The content of sterigmatocystin, the precursor of aflatoxin, was lower in the culture added with 50ppm allylisothicoyanate after 48 hours, however was rather higher after 144 hours compared to that of the control. The addition of allylisothiocyanate resulted in the increase of lipid, protein, RNA in mycelium and the content of citrate in the media, but the amount of AMP was low.

• Korean Journal of Medicinal Crop Science
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• v.10 no.3
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• pp.181-184
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• 2002

This study was conducted to select the optimum cultivar of wasabi in water condition. In three cultivar, Daruma, Simanesarai and Daihoichigo, the Daruma was superior to the others. Though total rhizome weight was similar to Daruma and Daihoichigo, rhizome of main stem of Daruma was bigger than that of Daihoichigo, specially distribute in $80{\sim}159g$ size. The allylisothiocyanate content was higher in rhizome than that of leaf, lateral rhizome and petiole. In three cultivar, the allylisothiocyanate content of rhizome, Daihoichigo, Daruma and Simanesairai was 0.3389, 0.6332, 0.3956(mg/g), respectively.

• Korean Journal of Medicinal Crop Science
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• v.11 no.3
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• pp.186-189
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• 2003

This experiment was conducted to evaluate the contents of allylisothiocyanate and the change of hardness of the rhizome part of Wasabia japonica Matsum. by months after planting in water cultivation. No difference was found in plant height and leaf length at 10th and 15th months after planting, respectively. The rhizome part of wasabi was cut half and the dry weight was weighed. Dry weight was obtained as 17.3% and 21.6% from fresh lower and upper part of rhizome at 10th month after planting. At 15th month after planting, dry weight was obtained as 21.2% and 22.3% from fresh lower and upper part of rhizome. The contents of allylisothiocyanate were 0.047 mg/g and 0.633 mg/g at 10th and 15th month, respectively.

• Korean Journal of Medicinal Crop Science
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• v.6 no.1
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• pp.1-5
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• 1998

We studied about the changes of Allylisothiocyanate (allyINCS) content in rhizome by growth period or season and the effect of foliar application of fertilizer on allylNCS content in Wasabia japonica. AllylNCS content in rhizome of Wasabia japonica was changed according to growth period or season and it was decreased suddenly in May, the last stage of flowering. Foliar application of 1 % urea increased rhizome weight, but had no effect on allylNCS content in rhizome. Total three times foliar applications of 1 % potassium sulfate from Feb. to April increased rhizome weight by 44% and allylNCS content by 38% compared with control and potassium sulfate was more effective than ammonium sulfate. Even though the use of 1 % urea and 1 % ammonium sulfate caused growth disorder due to high concentration, rhizome weight was similar to the control and allylNCS content of rhizome increased by 47% more than control.

• Korean Journal of Medicinal Crop Science
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• v.7 no.1
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• pp.31-36
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• 1999

This study was carried out to investigate the effect of sulfuric fertilizers on allylisothiocyanate(allylNCS) content of upland Wasabia japonica Matsum cultivated in heating condition in the winter season. The rhizome's growth characters following soil application of sulfur dust were not significantly different compared to control, though its soil acidity and available sulfur content was higher than other treatments. Total sulfur content of plant was increased by soil application as compared to foliar application and allylNCS content of rhizome increased up to 22.8% in the sulfur dust treatment. The result showed that soil application was effective for supplying $SO_4^{-2}$ in upland Wasabia japonica Matsum when cultivated in heating condition. In the analysis of correlation coefficients between growth and quality characteristics, allylNCS content of rhizome showed the significantly positive correlation with total sulfur content of shoot, rhizome and allylNCS content of shoot.

• Proceedings of the Korean Society of Crop Science Conference
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• 1997.10a
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• pp.122-123
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• 1997

• Culinary science and hospitality research
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• v.12 no.4 s.31
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• pp.131-139
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• 2006

This study carried out the standardization of processing conditions in mustard powder (MP) for quality improvement and suggested a recycling scheme of mustard oil(MO). Pungent taste in MP and MO was estimated using allylisothiocyanate (AITC) content as a marker. Recovery of crude oil from mustard seed (MS) was best by the cold pressing method. Residual AITC content at $30^{\circ}C$ pressing was 0.54% and 0.42% at $230^{\circ}C$. But residual AITC contents in MOs were 92ppm, 139ppm, respectively. The residual AITC content in MP was the highest (0.54%) when the moisture content in MP was 4.5%. The residual content of volatile oil in MP and MO showed similar results. In summary, crude oil must be removed from MS using the cold pressing method.

• Korean Journal of Medicinal Crop Science
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• v.12 no.6
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• pp.473-477
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• 2004

Wasabis (Wasabia japonica Matsum.) have been used as a spice with its petioles and rhizomes, but the production of its rhizomes in upland cultivation was very difficult for the severe damage of rotting disease during summer season. This experiment was carried out to increase the yield of marketable rhizome in wasabi when cultured in upland. Seedlings were raised in sub-alpine area for 7 and 12 months and then transplanted to experimental area on Sept. 20 and the yield was investigated on May 12 of the next year. The marketable rhizome(above 40g/plant) was produced in seedlings raised for 7 months. Production rate of marketable rhizome was 13.3% and total yield was 72.9 kg/10a. The content of allylisothiocyanate in rhizome of upland wasabi was 0.777 mg/g and its content was equal to wasabi cultured in water condition for 18 months. These results suggested that the upland cultivation of wasabi seedlings raised in sub-alpine area for 7 months was possible to product marketable rhizome.