• Archives of Orthopedic and Sports Physical Therapy
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• v.14 no.2
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• pp.91-97
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• 2018

Purpose: The purpose of this study was to observe the effects of sport massage and McKenzie stretch exercise on forward head posture. To understand the effect, this study measured and analyzed body alignment of the neck. Methods: In this study, a total of 14 subjects with forward head posture were analyzed. Subjects were divided into two groups: group 1 (n=7) received a McKenzie stretch exercise and group 2 (n=7) received a McKenzie stretch exercise and a sport massage. The McKenzie stretch exercise was performed three times a week, for four weeks, while the sport massage was performed once a week. The body alignment of the neck (CVA, CRA) was measured using a goniometer. Results: The improvements in neck body alignments (CVA, CRA) after the McKenzie stretch exercise and sport massage were both significant (group 1: p<0.05; group 2: p<0.05), whereas the between-group comparison found no significant differences. Conclusions: In conclusion, our study confirmed that the McKenzie stretch exercise and sport massage had a significant positive effect on the body alignment of the neck. However, a study that included a greater number of subjects and a long-term treatment period is needed to generalize these results.

• Journal of International Academy of Physical Therapy Research
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• v.12 no.2
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• pp.2331-2337
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• 2021

Background: More women than men experience genu recurvatum, which can lead to knee pain and arthritis if left unattended. Pilates combined with taping is a suitable rehabilitation method for women with genu recurvatum. Objectives: To aimed the effect of taping and Pilates stabilization exercise on physical alignment and improvement of genu recurvatum in women with genu recurvatum. Design: A Randomized controlled trial. Methods: Twenty-one women were divided into three groups: taping group (back of knee taping and quadriceps femoris taping, n=7), Pilates exercise group (hip, knee and abdominal muscles exercise, n=7), and combined group (taping with pilates exercise, n=7). The measured variables were sagittal plane alignment and back and abdominal muscle, knee flexor and extensor strength. Results: After the intervention, all sagittal plane alignments were significantly improved in both the Pilates and combined groups. Sagittal plane alignment was significantly improved in the combined group compared with the taping group. Back and abdominal muscle strength were significantly improved in the Pilates and combined groups compared with the taping group. Knee flexor and extensor were significantly improved in the combined group compared with the Pilates group and in the Pilates group compared with the taping group. Conclusion: Pilates exercise with taping or Pilates exercise alone was effective intervention methods to improve physical alignment and strength in women with genu recurvatum.

• Molecules and Cells
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• v.45 no.7
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• pp.495-501
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• 2022

Leucine dehydrogenase (LDH, EC 1.4.1.9) catalyzes the reversible deamination of branched-chain L-amino acids to their corresponding keto acids using NAD⁺ as a cofactor. LDH generally adopts an octameric structure with D4 symmetry, generating a molecular mass of approximately 400 kDa. Here, the crystal structure of the LDH from Pseudomonas aeruginosa (Pa-LDH) was determined at 2.5 Å resolution. Interestingly, the crystal structure shows that the enzyme exists as a dimer with C2 symmetry in a crystal lattice. The dimeric structure was also observed in solution using multiangle light scattering coupled with size-exclusion chromatography. The enzyme assay revealed that the specific activity was maximal at 60℃ and pH 8.5. The kinetic parameters for three different amino acid and the cofactor (NAD⁺) were determined. The crystal structure represents that the subunit has more compact structure than homologs' structure. In addition, the crystal structure along with sequence alignments indicates a set of non-conserved arginine residues which are important in stability. Subsequent mutation analysis for those residues revealed that the enzyme activity reduced to one third of the wild type. These results provide structural and biochemical insights for its future studies on its application for industrial purposes.

• Journal of the Korean Society of Industry Convergence
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• v.26 no.1
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• pp.201-209
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• 2023

Recently, fiber-reinforced composites have been widely used in various industrials fields. In this study, the mechanical behavior, especially fracture behavior, of biomimetic fiber-reinforced composites subjected to pressure loading was analyzed using finite element analysis (FEA). The fiber alignments in the biomimetic composites formed a helicoidal structure, wherein a stacking sequence involved a gradual rotation of each ply in the multi-layered laminated composites. For comparison, cross-ply composite samples with fibers arranged at 0° and 90° were prepared and analyzed. In addition, the mechanical behavior was analyzed based on combinations of the stacking sequence of carbon-fiber composites and glass-fiber composites. The FEA results showed that, when compared with the cross-ply samples, the mechanical properties of the biomimetic composites were considerably improved under pressure loading, which was applied to one side of the composites. Thus, the biomimetic helicoidal structure significantly improved the mechanical properties of the composites. Placing materials having high elasticity and strength in the outermost layers (the layer of the side on which pressure was applied and the opposite side layer) of the composites also significantly contributed to improving the mechanical properties of the composites.

• Journal of the Korea Society of Computer and Information
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• v.14 no.7
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• pp.151-160
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• 2009

The aim of this study is to propose economical driving speed index which those are geometric road status; assess the levels of which those cost-benefit of driving energy consumption and emission; are search road safety design and operational technology for driving simulator. For the objective, we analyzed the current status of driving energy consumption and driving scenarios by the road alignments, and reviewed driving and technical specifications by the geometric types of road according to the implementation, and extended completion. Throughout the result of this study, diverse related driving information provision service, efficiently navigation driving module is expected to be implemented in the national highway design system.

• Proceedings of the Korea Information Processing Society Conference
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• 2007.11a
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• pp.272-273
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• 2007

현대 유전체학 기술의 진보는 생물학적으로 중요한 의미를 갖는 생물들의 유전체 서열의 규명 genome sequencing)에 힘입은 바 크다. 기존의 유전체 서열결정법은 주로 염기변이율이 낮은 생물들에 초점을 맞추어 왔다. 하지만 염기변이율이 높은 생물들의 유전체 염기서열을 결정할 필요가 높아짐에 따라 이를 위한 방법론에 대한 연구가 활발히 진행되고 있다. 염기변이율이 높은 생물들의 이배체 (diploid) 유전체 서열이 효과적으로 결정될 수 있을 경우 기존의 유전체 서열비교의 방법론에도 변화가 요청되고 있는 실정이다. 기존의 유전체 서열비교 (whole-genome alignment) 방법론은 반수체 (haploid) 유전체들의 서열비교을 위해 개발되었지만, 염기변이율이 높은 생물들의 유전체 서열비교에는 반수체 유전체들 비교에 특화된 도구들이 필요하다. 또한 현재 서열비교를 시각화하는 소프트웨어들도 반수체 유전체 비교를 위해 개발된 실정이다. 본 논문의 목표는 이배체 유전체 서열을 비교하는 방법론을 개발을 용이하기위해 이배체 유전체의 서열을 생성하는 도구를 개발하는 것이다. 개발된 도구는 실제 일어날 수 있는 염기변이와 genomic rearrangement를 사용자의 입력을 받아 다수의 생물들의 유전체 서열을 생성해 낸다. 이를 통해 이배체 유전체 서열을 비교하는 도구의 개발을 용이하게 하는데 초첨을 맞추고 있다.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.8
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• pp.1169-1173
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• 2007

The protein encoded by SLC27A2 gene is an isozyme of long-chain fatty-acid-coenzyme A ligase family, and it converts free long-chain fatty acids into fatty acyl-CoA esters, and thereby plays a key role in lipid biosynthesis and fatty acid degradation. In the present study, SLC27A2 located on human chromosome 15 was selected as candidate gene and we isolated and cloned partial fragments of mRNA sequence and genomic fragments of porcine SLC27A2 gene. The coding region of the gene as determined by alignments shared 90% and 82% identity with human and mouse cDNAs, respectively. Detection in LargeWhite and Meishan breeds showed that a single nucleotide polymorphism (SNP) ($A{\rightarrow}G$) existed in exon 7, which caused corresponding amino acid changed for encoding. In LargeWhite pigs it encoded for Val while in Meishan pigs it encoded for Ile, so we developed the PCR-RFLP genotype method for detection of this polymorphism. Association study in 135 $F_2$ reference family indicated that significant correlation existed between the polymorphism and growth and carcass traits.

• Proceedings of the Korean Aquaculture Society Conference
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• 2003.10a
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• pp.134-135
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• 2003

The geographic expansion of the toxic dinoflagellates genus Alexandrium has been shown to be world wide ranging. The members of the genus Alexandrium ocnstituted of 20-30 species did not show substantial differences in their morphology, which is mostly referred in the 'tamarensis species complex', except some species. Though rDNA sequences variations are very few and pseudogene types are so diverse that it is difficult to use them as the specific markers. In this study, we outlined Korean and Japanese A, tamarense and A. catenella regional isolates by phylogenetic analysis inferred from no cutting alignments of LSU rDNA D1-D2 and SSU rDNA sequences to group these regional isolates. The results were compared to RFLP patterns of PCR products targeted chloroplast DNA. Lastly screening of highly repeated microsatellite DNA which is frequently used for population analysis in eukaryotes was conducted. A. catenella regional strains identified by the sequencing of rDNA D1-D2 domain were divided into at least 3 groups of type E, CMC and Chinese type, divergence root may not be deep comparing with that of A. tamarense whose pseudogenes are very variable. Results of RFLP pattern and the phylogeny of the unknown gene targeting chloroplast showed that Korean and Japanese A. catenella regional isolates were divided into 3 types: Korean, Japanese and the third CMC types. Population-specific PCR amplification with Japanese A. catenella type-specific PCR primers was useful method for population analysis of A. catenella. Various types of satellite sequences such as 5 nucleotides repeats were obtained from A. tamarense and A. catenella. The 5 nucleotides repeats were primed at the both 3'and 5' ends, and these repeats were prominent as longer repeated motifs. This repeated DNA was intercalated as internal sequences containing various types subrepeats. It is expected that these satellite DNA would be a useful molecular population marker through detail comparison among Alexandrium regional isolates to trace their transferring pathway and to prevent their human-associated their regional extents.

• Journal of Ginseng Research
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• v.37 no.3
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• pp.361-370
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• 2013

A lysine histidine transporter (LHT) cDNA was isolated and characterized from the roots of Panax ginseng, designated PgLHT. The cDNA is 1,865 bp with an open reading frame that codes for a protein with 449 amino acids and a calculated molecular mass of 50.6 kDa with a predicted isoelectric point of 8.87. Hydropathy analysis shows that PgLHT is an integral membrane protein with 9 putative membrane-spanning domains. Multiple sequence alignments show that PgLHT shares a high homology with other plant LHTs. The expression profile of the gene was investigated by real-time quantitative polymerase chain reaction during various chemical treatments. PgLHT was up-regulated in the presence of abscisic acid, salicylic acid, methyl jasmonate, NaCl, and amino acids. To further explore the function of PgLHT gene, full-length cDNA of PgLHT was introduced into P. ginseng by Agrobacterium rhizogenes A4. The overexpression of PgLHT in the hairy roots led to an obviously increase of biomass compared to the controls, and after addition of the amino acids, the overexpressed-PgLHT hairy roots grew more rapidly than untreated controls during early stage of the culture cycle. The results suggested that the PgLHT isolated from ginseng might have role in the environmental stresses and growth response.

• Journal of Advanced Marine Engineering and Technology
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• v.40 no.3
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• pp.185-190
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• 2016

Damages of the main engine aftmost bearing and the after stern tube bearing tend to increase due to misalignment. And as the shafting system becomes stiffer due to the large engine power, whereas the hull structure becomes more flexible due to optimization by using high tensile thin steel plates. And this is the reason that more sophisticated shaft alignments are required. In this study, the optimum shafting alignment calculation was carried out, considering the thermal expansion effect, exploiting the sensitivity index, which indicates the reasonable position of forward intermediate shaft bearing for shaft alignment. and as the main subject in this study, the elastic deformation on intermediate shaft and main engine bearings occurred by vertical load of shaft mass were examined thoroughly and analyzed allowable load of bearings, reaction influence numbers of all bearings. As the result, a reliable optimum shafting alignment was derived theoretically. To verify these results, they were referred to the engine maker's technical information of main engine installation and being used shafting alignment programs of both Korean Register of Shipping and Det Norske Veritas, their reliability were reviewed.