• 제목/요약/키워드: alcoholic extracts

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누룩추출물로 제조한 약주의 품질특성 (Quality Characteristics of Yakju with Nuruk Extracts)

  • 강지은;최한석;김재운;김찬우;여수환;정석태
    • 한국식품과학회지
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    • 제48권3호
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    • pp.223-230
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    • 2016
  • 본 연구에서는 약주의 이취와 갈변 원인물질을 알아보기 위해 제균한 누룩추출물을 첨가하여 약주를 제조하고 특성을 확인하였다. 알코올 함량은 재래누룩에서 16.33%, 개량누룩에서 18.67%로 개량누룩에서 1.1배 높게 나타났다. 산도(mL/10 mL)는 재래누룩 5.07, 개량누룩 8.32, 아미노산도 함량(mL/10 mL)도 각각 1.61, 4.72로 개량누룩이 약 2.9배 증가하였다. 당화력에 크게 영향을 미치는 글루코아밀레이스는 재래누룩에 비해 개량누룩이 3.2-44.0배 높은 활성을 보였고, 가용 고형물(r =0.965)과 색차(r =0.954)와 매우 높은 상관성을 보였다. 유기산은 숙신산과 젖산 함량이 가장 높게 나타났으며, 유리 질소화합물 함량(mg/L)은 재래누룩 540.0, 개량누룩 2285.9로 개량누룩에서 4.2배 높게 나타났다. 각 누룩추출물 약주의 이취 및 갈변은 유리질소화합물(r =0.539, 0.792)과 높은 상관성을 보였다.

Ethanol but not Aqueous Extracts of Tubers of Sauromatum Giganteum(Engl.) Cusimano and Hett Inhibit Cancer Cell Proliferation

  • Gao, Shi-Yong;Li, Jun;Wang, Long;Sun, Qiu-Jia;Gong, Yun-Fei;Gang, Jian;Su, Yi-Jun;Ji, Yu-Bin
    • Asian Pacific Journal of Cancer Prevention
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    • 제15권24호
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    • pp.10613-10619
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    • 2015
  • Background: Both alcohol and aqueous extracts of Sauromatum giganteum(Engl.) Cusimano and Hett, the dried root tuber of which is named Baifuzi in Chinese, have been used for folklore treatment of cancer in Northeast of China. However, little is known about which is most suitable to the cancer therapy. Materials and Methods: Serum pharmacology and MTT assays were adopted to detect the effects of ethanol and aqueous extracts of Sauromatum giganteum(Engl.) Cusimano and Hett, prepared by heat reflux methods, on proliferation of different cancer cells. Results: Cancer cells treated with medium supplemented with 10%, 20%, 40% serum(v/v) containing ethanol extract had a decline in viability, with inhibition rates of 7.69%, 21.8%, 41.9% in MCF-7 cells, 42.8%, 48.1%, 51.8% in SGC-7901 cells, 44.1%, 49.2%, 53.7% in SMMC-7721 cells, 6.8%, 15.2%, 39.8% in HepG2 cells, 7.57%, 16.3%, 36.2% in HeLa cells, 6.24%, 12.5%, 27.4% in A549 cells, and 7.20%, 17.5%, 31.3% in MDA-MB-231 cells, respectively. Viability in the aqueous extract groups was no different with that of controls. Conclusions: An ethanol extract of Sauromatum giganteum(Engl.) Cusimano and Hett inhibited the proliferation of SMMC-7721, SGC-7901 and MCF-7 cells, which supports the use of alcoholic but not aqueous extracts for control of sensive cancers, which might include hepatocarcinoma, gastric cancer and breast cancer.

Total Phenolic Compounds and Flavonoids in the Parts of Artichoke (Cynara scolymus L.) in Viet Nam

  • Thi, Bui Ha Thu;Park, Moon-Ki
    • 한국환경과학회지
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    • 제17권1호
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    • pp.19-27
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    • 2008
  • Artichoke extracts are widely used alone or in association with other herbs for embittering alcoholic and soft drinks and to prepare herbal teas or herbal medicinal products in Viet Nam. The objective of this paper was a screening of flavonoids and total phenolic compounds content in the parts of artichoke (Cynara scolymus L.) as flowers, leaves, roots, trunks, stumps, The total phenolic compounds and flavonoids in the parts of artichoke were extracted among 3 extraction methods as methanol extraction (EM1), mixing methanol and water method (EM2) and water extraction method (EM3). Total phenolic compounds and flavonoids were determined by UV/VIS, HPLC techniques. The apigenin 7-O-glucosides, cynarin, narirutin, gallic acid, caffeic acid were found as the main flavonoids constituents in all parts of artichoke. It showed that value of total phenolic compounds and flavonoids by EM3 were higher than that of total phenolic compounds and flavonoids by EM1 and EM2. Furthermore, the results of this study revealed that total phenolic compounds and flavonoids, obtained by these convenient extraction methods, may show the quick efficacy of artichoke in all respects of their quality and quantity.

Hepatoprotective and Antioxidative Effects of Alisma orientale

  • Rhew, Kwang-Yul;Choi, Hyuck-Jai;Kim, Nam-Jae;Lee, Jang-Hoon
    • Natural Product Sciences
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    • 제17권4호
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    • pp.285-290
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    • 2011
  • The rhizome of Alisma orientale Juzep (Alismataceae) has been used as a crude drug for diabetes, edema, inflammation and urinary disturbances in oriental medicine. Recent animal studies have shown that the extract of Alisma orientale rhizome (AOR) can potently lower high levels of serum lipids and improve insulin resistance, which are usually detected in patients and animals with non-alcoholic fatty liver disease. So, we studied the antioxidative effects of AOR extracts and fraction in vitro and their protective effects against acute hepatotoxicity induced by $CCl_4$ in vivo.. We then investigated the effects of each fraction on hepatotoxicity induced by tert-butyl hydroperoxide (t-BHP). DAOR (dichloromethane fraction of the Alisma orientale rhizome) scavenged free radicals and superoxide anions. DAOR protected against $CCl_4$ induced hepatotoxicity. DAOR had hepatoprotective and antioxidative effects against t-BHP-induced hepatotoxicity in HepG2 cells and in rats.

메칠오렌지에 의한 말레인산클로르페니라민의 분광광도 정량 (Spectrophotometric Determination of Chlorpheniramine Maleate with Methyl Orange)

  • 전인구
    • Journal of Pharmaceutical Investigation
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    • 제16권4호
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    • pp.139-147
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    • 1986
  • A singly charged methyl orange(MO) anion was found to be extracted with chlorpheniramine maleate(CPM) as a 1 : 1 complex in chloroform. Of various solvents, MO-chloroform system gave a yellow color for CPM, while in the absence of CPM, an organic phase showed almost no color. In this way, the spectrophotometric method was investigated for the determination of CPM by solvent extraction. The addition of alcoholic bolic acid solution to the solvent extract gave a higher color stability and transparency at least 5 days, but the extract alone lost its color intensity significantly. CPM is determined by measuring the absorbance of the extracts over a range of $1{\sim}7{\times}10^{-4}M\;(39{\sim}273\;{\mu}g/ml)$ in aqueous solution at 423 nm. The molar absorptivity was $2.26{\times}10^3\;l,\;mol^{-1},\;cm^{-1}$. The absorbance of the extract was constant in the range of pH $3.7{\sim}4.6$. This novel method was applied for the determination of CPM in artificial and commercial preparations in comparison with the analytical method of CPM tablets in K.P.IV. The results obtained showed that the former was better in accuracy and time consumption than the latter.

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Effects of carbohydrase on phenolic acid and antioxidant activity of brown rice flour

  • Cho, Dong-Hwa;Park, Hye-Young;Lee, Seuk-Ki;Choi, Hye-Sun;Park, Jiyoung;Oh, Sea-Kwan
    • 한국작물학회:학술대회논문집
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    • 한국작물학회 2017년도 9th Asian Crop Science Association conference
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    • pp.270-270
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    • 2017
  • Brown rice flour (BRF) was treated with different carbohydrases (Viscozyme, Termamyl, Celluclast, AMG, Ultraflo, and Pentopan), and then aqueous alcoholic extracts (70% ethanol) from the treated RBF were examined for their phenolic compositions and antioxidant activities (ABTS and DPPH radical scavenging activity). All the carbohydrases tested induced significant increases in ABTS radical scavenging activity (2.1-3.0 times). Moreover, These enzymes increased the amount of extractable free phenolic acids by 10-15 times, especially for ferulic and p-coumaric acid. Among the enzymes tested, Pentopan which was active in arabinoxylan hydrolysis appeared to be most effective in increasing the free phenolic acid content and ABTS radical scavenging activity than other enzymes. Enzymatic hydrolysis of cell wall polysaccharides in BRF could be used as an effective procedure for raising the amount of extractable phenolic acids and thus increasing the antioxidant activity of BRF extract.

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INHIBITORY ACTION OF PROCESSED HERBAL MEDICINES ON THE PRODUCTION OF ADVANCED GLYCATION ENDPRODUCTS(AGEs)

  • Kim, Jin-Sook;Ko, Jin-Hee;Kim, Hyung-Jeong;Ma, Jin-Yeul
    • 대한약학회:학술대회논문집
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    • 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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    • pp.383.2-383.2
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    • 2002
  • Diabetic nephropathy is major chronic complication of diabetes mellitus. Advanced glycation endproducts(AGEs) are largely involved in the pathogenesis of diabetic nephropathy. The irreversibly formed AGEs do not return to normal even if hyperglycemia is corrected and continue to accumulate over the lifetime of protein. The AGEs inhibitor. aminoguanidine(AG), is the only protein glycation inhibitor currently under development. its safety however is desirable. To find possible AGEs inhibitor in herbal medicines, bovine serum albumin was added to a mixture of sugars and some of processed. unprocessed herbal medicines or AG. Cyperi rhizoma was processed in four different methods according to chinese pharmacopoeia and traditional literatures. In comparision to the negative control with no inhibitor and positive control with AG. alcoholic extracts of these processed cyperi rhizoma proved to have more potent inhibitory activities than that of unprocessed cyperi rhizoma. These results revealed that some processed herbal medicines have a more potent in vitro inhibitory action on AGEs formation than AG. suggesting the possible candidate for diabetic nephropathy from the processed herbal medicines.

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감홍로주 제조에 사용하는 재료 침출액의 항산화 효과 (Antioxidant Activities of the Extracts of Herbs Used for Gamhongro-ju)

  • 이새롬;신혜현;황금택;정석태;김태영
    • 한국식품영양과학회지
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    • 제39권5호
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    • pp.643-647
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    • 2010
  • 전통 향약술의 하나인 감홍로주의 효능을 좀 더 과학적으로 탐구하기 위하여 감홍로주를 만드는데 사용되는 재료의 침출액들의 항산화능을 알아보고자 하였다. 알코올 함량 45%, 60%의 정제주정(PE), 45% 재래식 증류주정(TDE)을 이용하여 주정 1000 mL에 계피, 방풍, 정향, 생강, 자초, 감초, 진피, 용안육을 각각 2.5 g씩 혼합하여 넣고 침출하였다. 알코올 함량 60%의 PE에 재료를 20 g씩 따로 넣고 침출하였다. 상온에서 90일 동안 침출하면서 30일마다 항산화능을 조사하였다. 항산화능은 폴리페놀 함량 및 ABTS와 DPPH radical scavenging activity를 이용하여 측정하였다. 8가지 재료의 혼합 침출액의 총 폴리페놀 함량은 45%와 60% PE에서 30일 동안 침출했을 때보다 90일 동안 침출했을 때에 유의적으로 증가하였다. 8가지 재료를 각각 따로 침출하여 만든 침출액 중에서는 정향 침출액이 다른 시료들에 비해 폴리페놀의 함량이 유의적으로 높았다. ABTS $IC_{50}$ 값은 45%와 60% PE와 45% TDE 침출액에 30일 동안 침출했을 때보다 90일 동안 침출했을 때 유의적으로 그 값이 감소하였다. 이 실험을 통해 침출 조건에 관계없이 대체적으로 침출기간이 길어질수록 항산화능이 증가한다는 것을 알 수 있었다. 8가지 재료 침출액 중 ABTS $IC_{50}$ 값은 정향, 계피, 생강침출액 순으로 낮았다. DPPH $IC_{50}$ 값은 정향, 계피, 감초 침출액 순으로 낮았다. 생강 침출액은 총 폴리페놀 함량이 가장 낮았으나, ABTS와 DPPH 실험에서는 높은 항산화능을 보여 폴리페놀 물질 이외에도 항산화능에 관여하는 물질이 존재하는 것으로 보인다. 이 실험을 통해 감홍로주 제조에 사용되는 재료 중 정향과 계피가 가장 항산화능이 높은 재료라는 것을 알 수 있었다.

Inhibitory Effects of Ethanol Extracts from Nuruk on Oxidative Stress, Melanogenesis, and Photo-Aging

  • Lee, Sang-Jin;Cho, Sung-Won;Kwon, Yi-Young;Kwon, Hee-Suk;Shin, Woo-Chang
    • Mycobiology
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    • 제40권2호
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    • pp.117-123
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    • 2012
  • Nuruk contributes to the unique characteristics of Korean alcoholic beverages. In this study, the effects of nuruk extracts (NE) on anti-oxidant characters, melanogenesis, and anti-photoaging activity were investigated. NEs were obtained from the 70% ethanol extracts of six types of nuruk, which have been used in brewing of fermented alcohol beverages in Korea. First, various antioxidant characteristics were identified in terms of 2,2'-azino-bis(3-ethylbenzthiozoline-6-sulphonic acid) (ABTS) radical scavenging activity, superoxide dismutase (SOD) expression, and inhibition of xanthine oxidase activity. NE#4 exhibited potent ABTS radical scavenging activity ($IC_{50}$ = 19.51 ${\mu}g$/mL). Compared with NE#4, relatively lower levels of activity were observed for NE#3 and NE#6, with $IC_{50}$ values of 90.99 and 76.88 ${\mu}g$/mL, respectively. According to results of western blot analysis for determination of SOD expression in $H_2O_2$-treated HepG2 cells, NE#5 and NE#6 induced a dramatic increase in the expression ratio of SOD, compared to the group treated with $H_2O_2$ only. Activity of xanthine oxidase, which converts xanthine into uric acid, generating superoxide ions, was inhibited by NE#4 and NE#6 in a dose-dependent manner. NE#4 induced significant inhibition of mushroom tyrosinase activity. A reduction in cellular melanin contents of 80% was observed in B16F1 melanocytes treated with NE#5 and NE#6; these effects were similar to those of arbutin at 100 ${\mu}M$. In addition, gelatin zymography and reverse transcription-PCR analysis were performed for assessment of anti-photoaging activity of Nuruk. Treatment with NE#6 resulted in dramatically inhibited activities of matrix metalloproteinase (MMP)-2/-9, suppressed expression of MMP-1, and increased expression of type-1 procollagen. Results of gelatin zymography for NE#4 and NE#5 were similar, to a slightly lesser degree. These results suggest the potential of NE#4 and NE#6 as natural ingredients for use in functional foods and cosmetics.

올레산 유도 비알코올성 지방간세포에서 자색옥수수 색소 1호 포엽과 속대 추출물의 지질 축적 억제 효과 (Inhibitory Effect of Purple Corn 'Seakso 1' Husk and Cob Extracts on Lipid Accumulation in Oleic Acid- Induced Non-Alcoholic Fatty Liver Disease HepG2 Model)

  • 이기연;김태희;김재은;배선화;박아름;이효영;최성진;박종열;권순배;김희연
    • 한국식품위생안전성학회지
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    • 제35권1호
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    • pp.93-101
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    • 2020
  • 연구의 목적은 oleic acid로 지방생성이 유도된 HepG2 세포에서 자색옥수수 색소 1호 포엽 및 속대 추출물이 간세포 내 지방생성에 미치는 영향을 구명하는 것이다. 자색옥수수 색소 1호 포엽 및 속대 추출물에 의한 HepG2 세포 내 지방 축적의 변화를 확인하기 위하여 배양된 세포에 oleic acid로 지방 축적을 유도하고 추출물에 의한 중성지방생성 억제 효과를 측정하였으며 추출물을 처리하지 않은 대조군과 추출물을 처리한 실험군의 지방합성 및 축적에 관련된 유전자와 단백질 발현량을 RT-PCR과 Western blot을 통하여 측정하였다. Oil Red O와 Nile Red 염색을 통하여 추출물의 처리로 HepG2 세포 내 중성지방 축적이 억제된 것을 확인하였다. RT-PCR에 의하여 mRNA 발현량을 측정한 결과, oleic acid에 의하여 지방 생성이 유도된 대조군에 비하여 모든 추출물 처리군의 SREBP-1c와 SREBP-1a 유전자 발현량이 유의적으로 감소되었다. Western blot을 실시하여 p-AMPK, p-SREBP1, PPARα, FAS 단백질의 발현량을 측정한 결과, 간에서 지질대사에 관여하는 주요 인자인 SREBP1 단백질의 발현은 추출물의 처리 농도에 따라 유의하게 감소하였으며 지방산의 생합성 경로에 관여하는 주요 효소인 FAS의 단백질 발현향은 모든 처리 농도에서 현저하게 감소된 것이 확인되었다. 본 연구결과는 자색옥수수 색소 1호 포엽 및 속대 추출물이 간세포 내에서 중성지방의 축적을 억제시키고 지질 합성에 관련된 유전자 및 단백질의 발현을 억제시킴으로써 간 세포 내 지질 축적을 완화할 수 있는 기능성 소재로의 활용가치가 높다고 판단된다.