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Microbiological Hazard Analysis for HACCP System Application to Vinegared Pickle Radishes (식초절임 무의 HACCP 시스템 적용을 위한 미생물학적 위해 분석)

  • Kwon, Sang-Chul
    • Journal of Food Hygiene and Safety
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    • v.28 no.1
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    • pp.69-74
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    • 2013
  • This study has been performed for 150 days from February 1 - June 31, 2012 aiming at analyzing biologically hazardous factors in order to develop HACCP system for the vinegared pickle radishes. A process chart was prepared as shown on Fig. 1 by referring to manufacturing process of manufacturer of general vinegared pickle radishes regarding process of raw agricultural products of vinegared pickle radishes, used water, warehousing of additives and packing material, storage, careful selection, washing, peeling off, cutting, sorting out, stuffing (filling), internal packing, metal detection, external packing, storage and consignment (delivery). As a result of measuring Coliform group, Staphylococcus aureus, Salmonella spp., Bacillus cereus, Listeria Monocytogenes, E. coli O157:H7, Clostridium perfringens, Yeast and Mold before and after washing raw radishes, Bacillus cereus was $5.00{\times}10$ CFU/g before washing but it was not detected after washing and Yeast and Mold was $3.80{\times}10^2$ CFU/g before washing but it was reduced to 10 CFU/g after washing and other pathogenic bacteria was not detected. As a result of testing microorganism variation depending on pH (2-5) of seasoning fluid (condiment), pH 3-4 was determined as pH of seasoning fluid as all the bacteria was not detected in pH3-4. As a result of testing air-borne bacteria (number of general bacteria, colon bacillus, fungus) depending on each workplace, number of microorganism of internal packing room, seasoning fluid processing room, washing room and storage room was detected to be 10 CFU/Plate, 2 CFU/Plate, 60 CFU/Plate and 20 CFU/Plate, respectively. As a result of testing palm condition of workers, as number of general bacteria and colon bacillus was represented to be high as 346 $CFU/Cm^2$ and 23 $CFU/Cm^2$, respectively, an education and training for individual sanitation control was considered to be required. As a result of inspecting surface pollution level of manufacturing facility and devices, colon bacillus was not detected in all the specimen but general bacteria was most dominantly detected in PP Packing machine and Siuping machine (PE Bulk) as $4.2{\times}10^3CFU/Cm^2$, $2.6{\times}10^3CFU/Cm^2$, respectively. As a result of analyzing above hazardous factors, processing process of seasoning fluid where pathogenic bacteria may be prevented, reduced or removed is required to be controlled by CCP-B (Biological) and threshold level (critical control point) was set at pH 3-4. Therefore, it is considered that thorough HACCP control plan including control criteria (point) of seasoning fluid processing process, countermeasures in case of its deviation, its verification method, education/training and record control would be required.

Evaluation for Application of IOM Sampler for Agricultural Farmer's Inhalation Exposure to Kresoxim-methyl and Fenthion (농작업자의 Kresoxim-methyl과 fenthion에 대한 호흡노출량 측정을 위한 IOM 채집기의 효율성 평가)

  • Lee, Jiho;Kim, Eunhye;Lee, Jonghwa;Shin, Yongho;Maasfeld, Wolfgang;Choi, Hoon;Moon, Joon-Kwan;Lee, Hyeri;Kim, Jeong-Han
    • The Korean Journal of Pesticide Science
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    • v.19 no.3
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    • pp.230-240
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    • 2015
  • An IOM sampler equipped with glass fiber filter has been recently utilized instead of solid adsorbent, which was used to measure the inhalation exposure of agricultural operator to pesticides. The aim of this study is to validate the efficacy of an IOM sampler by measuring the trapping efficiency and breakthrough using kresoxim-methyl water-dispersible granule and fenthion emulsifiable concentrate. On LC-MS/ MS, minimum detection level was 12.5 pg and method limit of detection was 5.0 ng/mL. Good linearity ($R^2$ > 0.999) for matrix matched standards was obtained. Recoveries of pesticides from glass fiber filter were 102-109% (kresoxim-methyl) and 97-104% (fenthion) while those from XAD-2 resin were 94-98% (kresoxim methyl) and 93-100% (fenthion). Trapping efficiency test was performed with personal air pumps and IOM sampler (glass fiber filter) connected with solid adsorbent (XAD-2 resin) with two types of formulation (solid and liquid) which were diluted by standard rate and sprayed to IOM sampler. Those pesticides were trapped only in glass fiber filter without any breakthrough to solid adsorbent. After spiking of pesticides to glass fiber filter, breakthrough test was carried out with IOM sampler (glass fiber filter) which was connected with solid adsorbent. As a results, 87-101% of kresoxim-methyl and 96-105% of fenthion remained in spiked glass fiber filter, however, no pesticides were detected in second glass fiber filter and solid adsorbent. In conclusion, IOM sampler which equipped with glass fiber filter can be applied widely for pesticide inhalation exposure study since it has good trapping efficiency and adsorption capacity, regardless of the solid or liquid formulation.

Suggestions for improving data quality assurance and spatial representativeness of Cheorwon AAOS data (철원 자동농업기상관측자료의 품질보증 및 대표성 향상을 위한 제언)

  • Park, Juhan;Lee, Seung-Jae;Kang, Minseok;Kim, Joon;Yang, Ilkyu;Kim, Byeong-Guk;You, Keun-Gi
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.20 no.1
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    • pp.47-56
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    • 2018
  • Providing high-quality meteorological observation data at sites that represent actual farming environments is essential for useful agrometeorological services. The Automated Agricultural Observing System (AAOS) of the Korean Meteorological Administration, however, has been deployed on lawns rather than actual farm land. In this study, we show the inaccuracies that arise in AAOS data by analyzing temporal and vertical variation and by comparing them with data recorded by the National Center for AgroMeteorology (NCAM) tower that is located at an actual farming site near the AAOS tower. The analyzed data were gathered in August and October (before and after harvest time, respectively). Observed air temperature and water vapor pressure were lower at AAOS than at NCAM tower before and after harvest time. Observed reflected shortwave radiation tended to be higher at AAOS than at NCAM tower. Soil variables showed bigger differences than meteorological observation variables. In August, observed soil temperature was lower at NCAM tower than at AAOS with smaller diurnal changes due to irrigation. The soil moisture observed at NCAM tower continuously maintained its saturation state, while the one at AAOS showed a decreasing trend, following an increase after rainfall. The trend changed in October. Observed soil temperature at NCAM showed similar daily means with higher diurnal changes than at AAOS. The soil moisture observed at NCAM was continuously higher, but both AAOS and NCAM showed similar trends. The above results indicate that the data gathered at the AAOS are inaccurate, and that ground surface cover and farming activities evoke considerable differences within the respective meteorological and soil environments. We propose to shift the equipment from lawn areas to actual farming sites such as rice paddies, farms and orchards, so that the gathered data are representative of the actual agrometeorological observations.

The Comparative Studies on the Terrestrial Insect Diversity in Protected Horticulture Complex and Paddy Wetland (시설원예단지와 논습지의 육상곤충 다양성 비교분석)

  • Son, Jin-Kwan;Kong, Min-Jae;Kang, Dong-Hyeon;Kang, Bang-hun;Yun, Sung-Wook;Lee, Si-Young
    • Journal of Wetlands Research
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    • v.18 no.4
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    • pp.386-393
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    • 2016
  • Agricultural ecosystem is recognized as a space for providing a variety of services, in addition to the food production that it originally encompassed, such as water purification, biological habitat, air purification, soil conservation, and landscape development. The construction of greenhouses in agricultural landscapes can cause deterioration of ecosystem services because of the increase of impermeable area and loss of biological habitats. This study aimed to compare insect diversity between different types of constructed greenhouses and paddy ecosystems. The target study area was selected by considering the distribution status of horticultural complexes and was classified as Single Vinyl Greenhouse, Multi Vinyl Greenhouse or Glass Greenhouse and they were compared with four paddies. The study locations were in Gu-Mi, Bu-Yeo, Ginje and Jin-Ju. A total of 2,333 individual insects belonging to 9 orders, 38 families, 76 genus, and 80 species were collected. The composition of orders was Hemiptera (22.37%), Coleoptera (18.42%), Hymenoptera (14.47%), Orthoptera (11.84%), and Diptera (10.53%). The average number of collected species were in the order Paddy (39.38 species) > Single Vinyl Greenhouse (35.50 species) > Multi Vinyl Greenhouse (22.50 species) > Glass Greenhouse (24.00 species). The Diversity Index (H') was Paddy (4.76) > Single Vinyl Greenhouse (4.57) > Multi Vinyl Greenhouse (4.12), and Glass Greenhouse (4.12). The Richness Index (RI) was Paddy (7.72) and Single Vinyl Greenhouse (7.03) > Multi Vinyl Greenhouse (4.99) and Glass Greenhouse (5.32). From our results, it can be seen that the biological diversity features of insects decreased when greenhouses are constructed.However, Single Vinyl Greenhouse is noted to promote insect diversity more than that by Multi Vinyl Greenhouse and Glass Greenhouse. Hence, when constructing greenhouses, it is necessary to consider insect habitat to conserve insect diversity.

Diagnostic assessment on vegetation damage due to hydrofluoric gas leak accident and restoration planning to mitigate the damage in a forest ecosystem around Hube Globe in Gumi (구미 휴브글로브 주변 삼림생태계에서 불화수소가스 유출 사고에 기인한 식생피해 진단 및 그 피해를 완화시키기 위한 복원 계획)

  • Kim, Gyung Soon;An, Ji Hong;Lim, Chi Hong;Lim, Yun Kyung;Jung, Song Hie;Lee, Chang Seok
    • Journal of Wetlands Research
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    • v.17 no.1
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    • pp.45-52
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    • 2015
  • We obtained the following results from investigation on vegetation damage from 5 to 6 August, 2013, about one year after an accident that hydrofluoric acid leaked from a chemical maker, Hube Globe in Gumi. Pinus densiflora and Pinus strobus showed very severe damage. Ginko biloba, Quercus acutissima, Pinus rigida, Salix glandulosa, Hibiscus syriacus, and Lagerstroemia indica showed severe damage. Quercus variabilis, Lespedeza cyrtobotrya, and Miscanthus sinensis showed moderate damage. Quercus aliena, Smilax china, Arundidinella hirta, Ailanthus altissima, Robinia pseudoacacia, and Paulowinia coreana showed slight damage. We did not find any plants without leaf damage around there. This result means that fluoride damage still persists in this area as was known that fluoride remains for a long time in air, soil and water and exerts negative effects at all levels of an ecosystem. In addition, fluoride content contained in plant leaf depended on the distance from a fertilizer producing factory and vegetation damage tended to proportionate to the concentration in the Yeocheon industrial complex. In these respects, a measure for removal or detoxification of the remaining fluoride is urgently required around the hydrofluoric acid leak spot. Fertilizing of dolomite containing Ca and Mg, which can trap fluoride, was prepared as one of the restoration plans. In addition, phosphate fertilizing was added in order to enhance soil ameliorating effects. Furthermore, we recommend the introduction of tolerant plants as the second measure to mitigate fluoride damage. As the tolerant plants to make a new forest by replacing trees died due to hydrofluoric acid gas damage, we recommended Q. aliena and S. china, A. hirta, etc. were recommended as plant species to add mantle vegetation to the forest margin to ensure stable interior environment of the forest.

Analysis of Holdup Characteristics of Large and Small Bubbles in Three-Phase Fluidized Beds by using a Dynamic Gas Disengagement Method (삼상유동층에서 동력학적 기체유출 측정방법에 의한 큰 기포와 작은 기포의 체류량 특성 해석)

  • Lim, Hyun Oh;Lim, Dae Ho;Seo, Myung Jae;Kang, Yong;Jung, Heon;Lee, Ho Tae
    • Korean Chemical Engineering Research
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    • v.49 no.5
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    • pp.605-610
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    • 2011
  • Phase holdup characteristics of relatively large and small bubbles were investigated in a three-phase(gasliquid-solid) fluidized bed of which diameter was 0.105 m(ID) and 2.5 m in height, respectively. Effects of gas(0.01~0.07 m/s) and liquid velocities(0.01~0.07 m/s) and particle size($0.5{\sim}3.0{\times}10^{-3}m$) on the holdups of relatively large and small bubbles were determined. The holdups of two kinds of bubbles in three phase fluidized beds were estimated by means of static pressure drop method with the knowledge of pressure drops corresponding to each kind of bubble, respectively, which were obtained by dynamic gas disengagement method. Dried and filtered air which was regulated by gas regulator, tap water and glass bead of which density was $2500kg/m^3$ were served as a gas, a liquid and a fluidized solid phase, respectively. The two kinds of bubbles in three-phase fluidized beds, relatively large and small bubbles, were effectively detected and distinguished by measuring the pressure drop variation after stopping the gas and liquid flow into the column as a step function: The increase slope of pressure drop with a variation of elapsed time was quite different from each other. It was found that the holdup of relatively large bubbles increased with increasing gas velocity but decreased with liquid velocity. However, the holdup showed a local minimum with a variation of size of fluidized solid particles. The holdup of relatively small bubbles increased with an increase in the gas velocity or solid particle size, while it decreased slightly with an increase in the liquid velocity. The holdups of two kinds of bubbles were well correlated in terms of operating variables within this experimental conditions, respectively.

Effects of Column Diameter on the Holdups of Bubble, Wake and Continuous Liquid Phase in Bubble Columns with Viscous Liquid Medium (점성액체 기포탑에서 탑의 직경이 기포, wake 및 연속액상 체류량에 미치는 영향)

  • Lim, Dae Ho;Jang, Ji Hwa;Kang, Yong;Jun, Ki Won
    • Korean Chemical Engineering Research
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    • v.49 no.5
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    • pp.582-587
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    • 2011
  • Holdup characteristics of bubble, wake and continuous liquid phases were investigated in bubble columns with viscous liquid media. Effects of column diameter(0.051, 0.076, 0.102 and 0.152 m ID), gas velocity($U_G$=0.02~0.16 m/s) and liquid viscosity(${\mu}_L$=0.001~0.050 $Pa{\cdot}s$) of continuous liquid media on the holdups of bubble, wake and continuous liquid phases were discussed. The three phase such as bubble, wake and continuous liquid phases were classified successfully by adapting the dual electrical resistivity probe method. Compressed filtered air and water or aqueous solutions of CMC(Carboxy Methyl Cellulose) were used as a gas and a liquid phase, respectively. To detect the wake as well as bubble phases in the bubble column continuously, a data acquisition system(DT 2805 Lab Card) with personal computer was used. The analog signals obtained from the probe circuit were processed to produce the digital data, from which the wake phase was detected behind the multi-bubbles as well as single bubbles rising in the bubble columns. The holdup of bubble and wake phases decreased but that of continuous liquid media increased, with an increase in the column diameter or liquid viscosity. However, the holdup of bubble and wake phases increased but that of continuous media decreased with an increase in the gas velocity. The holdup ratio of wake to wake to bubble phase decreased with an increase in the column diameter or gas velocity, however, increased with an increase in the viscosity of con-tinuous liquid media. The holdups of bubble, wake and continuous liquid media could be correlated in terms of operating variables within this experimental conditions as: ${\varepsilon}_B=0.043D^{-0.18}U_G^{0.56}{\mu}_L^{-0.13}$, ${\varepsilon}_W=0.003D^{-0.85}U_G^{0.46}{\mu}_L^{-0.10}$, ${\varepsilon}_C=1.179D^{0.09}U_G^{-0.13}{\mu}_L^{0.04}$.

Field Survey on Smart Greenhouse (스마트 온실의 현장조사 분석)

  • Lee, Jong Goo;Jeong, Young Kyun;Yun, Sung Wook;Choi, Man Kwon;Kim, Hyeon Tae;Yoon, Yong Cheol
    • Journal of Bio-Environment Control
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    • v.27 no.2
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    • pp.166-172
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    • 2018
  • This study set out to conduct a field survey with smart greenhouse-based farms in seven types to figure out the actual state of smart greenhouses distributed across the nation before selecting a system to implement an optimal greenhouse environment and doing a research on higher productivity based on data related to crop growth, development, and environment. The findings show that the farms were close to an intelligent or advanced smart farm, given the main purposes of leading cases across the smart farm types found in the field. As for the age of farmers, those who were in their forties and sixties accounted for the biggest percentage, but those who were in their fifties or younger ran 21 farms that accounted for approximately 70.0%. The biggest number of farmers had a cultivation career of ten years or less. As for the greenhouse type, the 1-2W type accounted for 50.0%, and the multispan type accounted for 80.0% at 24 farms. As for crops they cultivated, only three farms cultivated flowers with the remaining farms growing only fruit vegetables, of which the tomato and paprika accounted for approximately 63.6%. As for control systems, approximately 77.4% (24 farms) used a domestic control system. As for the control method of a control system, three farms regulated temperature and humidity only with a control panel with the remaining farms adopting a digital control method to combine a panel with a computer. There were total nine environmental factors to measure and control including temperature. While all the surveyed farms measured temperature, the number of farms installing a ventilation or air flow fan or measuring the concentration of carbon dioxide was relatively small. As for a heating system, 46.7% of the farms used an electric boiler. In addition, hot water boilers, heat pumps, and lamp oil boilers were used. As for investment into a control system, there was a difference in the investment scale among the farms from 10 million won to 100 million won. As for difficulties with greenhouse management, the farmers complained about difficulties with using a smart phone and digital control system due to their old age and the utter absence of education and materials about smart greenhouse management. Those difficulties were followed by high fees paid to a consultant and system malfunction in the order.

Effect of High Pressure Processing on Freshness of Meat Products (육류가공품의 고압처리가 신선도에 미치는 영향 평가)

  • Hwang, Seong-Hee
    • Journal of Food Hygiene and Safety
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    • v.33 no.4
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    • pp.272-279
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    • 2018
  • The high pressure processing (HPP) is a technology which can preserve the quality of foods, such as the fresh taste, incense, texture, vitamin content, and so on, by minimizing the heating process. It does so by applying an instantaneous and uniform pressure that is the same as the water pressure that is 60 km deep in the sea. HPP is a technology that can inhibit food poisoning and spoilage caused by microorganisms and is currently an actively studied area. In this study, we investigated the effects of a high pressure treatment (0, 4, 6 min) on sliced ham, which is a typical meat product, at 600 MP a were tested for their effect on freshness. Moisture contents varied from 48 to 69%, salinity varied from 1.07 to 1.11%, and the pH decreased from 6.4~6.5 to 6.1~5.15. However, there was no difference between the control and treatment groups. General bacteria stored at $20^{\circ}C$ after hyper-pressure treatment were found to have no significant microorganisms in all groups until 4 weeks. but exceeded $10^5$ in control group and HPP 6 min treatment group from 5 weeks, At week 7, it was found to exceed $10^6$. The results indicate it was not possible to ingest food in the 4-and 6 minute treatment groups. Coliform was not observed in all groups despite observing for a total of 7 weeks at $20^{\circ}C$ weight test. VBN, a method used to determine the protein freshness of meat, showed a VBN value of less than 1 mg% until the fourth week and a value of 1 to 2 mg% after 5 weeks. The TBA was used as an index of the degree of fat acidosis in the meat tissues. The results showed it was below 0.18 mgMA / kg until the end of 7 weeks; this value was within the range for fresh meat, and there was no difference in treatment group. In this experiment, deformation of the packaging material did not occur and no swelling occurred due to the generation of gas. It is believed that the basic preservation effect was achieved only by blocking with the air due to the close contact of the packaging material.

Microbiological and Chemical Changes of Complete Feed during Spoilage (배합사료의 부패 동안 발생하는 미생물학적 및 영양학적 변화)

  • Yi, Kwonjung;Yeon, Jae-Sung;Kim, Juhyeon;Kim, Sam Churl;Moon, Hyung-In;Jeon, Che Ok;Lee, Sang Suk;Kim, Dong-Woon;Kim, Soo-Ki
    • Journal of Life Science
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    • v.25 no.10
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    • pp.1148-1155
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    • 2015
  • Commercial complete feeds contain enough nutrients to support animal growth and it is easy to be spoiled under proper temperature and humid conditions. The aim of this study was to investigate microbiological and chemical changes on complete feed for milking cow under open-air exposure with moisture 33% at 30℃ during 15 days. pH decreased 6.29 to 4.66 and water activity decreased gradually 0.99 to 0.95. Bacteria increased 6.2×106~1.6×107 to 2.1×109 CFU/g at 5 days and showed 108 CFU/g until 15 days. Fungi increased 103 CFU/g to 8.0×104 CFU/g. During the processing of spoilage, bacteria such as Acinetobacter oleivorans, Pediococcus acidilactici, Acinetobacter oleivorans, Weissella cibaria, and Methylobacterium komagatae were identified and fungi such as Fusarium sp. and Mucor sp. were also identified. Moisture content increased until 10 days (p<0.01). Crude protein was not changed so much whereas crude fat decreased 6.0% to 5.5% (p<0.01). Crude fiber and crude ash changed 2.0~ 3.0% and 4.5~ 4.8% levels with no significance, respectively. Gross energy was not almost changed at 4,400 kcal/g. During spoilage, lactate and propionate increased whereas acetate was not detected. Protease and lipase activities increased significantly during spoilage (p<0.01). Zearalenone content increased 59.2 μg/kg to 623.8 μg/kg, showing 10.5 times more production. During feed spoilage, pH decreased with microbial growth and various chemical changes were occurred.