• Journal of Microbiology and Biotechnology
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• 제25권6호
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• pp.828-836
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• 2015

Based on its α-amylase activity at pH 5.0 and optimal pH of the crude enzyme, a strain (named B-5) with acid α-amylase production was screened. The B-5 strain was identified as Bacillus amyloliquefaciens through morphological, physiological, and biochemical characteristics analysis, as well as 16S rDNA phylogenetic analysis. Its α-amylase gene of GenBank Accession No. GU318401 was cloned and expressed in Escherichia coli. The purified recombinant α-amylase AMY-Ba showed the optimal pH of 5.0, and was stable at a pH range of 4.0-6.0. When hydrolyzing soluble starch, amylose, and amylopectin, AMY-Ba released glucose and maltose as major end products. The α-amylase AMY-Ba in this work was different from the well-investigated J01542-type α-amylase which also came from B. amyloliquefaciens. AMY-Ba exhibited notable adsorption and hydrolysis ability towards various raw starches. Structure analysis of AMY-Ba suggested the presence of a new starch-binding domain at its C-terminal region.

• Journal of Biosystems Engineering
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• 제27권6호
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• pp.565-572
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• 2002

In order to control the root-zone temperature of greenhouse crops in the hydroponics at hot and cold season, heat pump system for cooling and heating was built and tested in this work. The system was air-to-water type and vapour compression type. The heating and cooling mode was selected by the four way valve. Capacity of the compressor was 3.75㎾ and heat transfer area of the evaporator and the condenser were 3.05㎡ and 0.6㎡, respectively. According to the performance test, it could supply heat of 42,360 to 64,372kJ/h depending on the water circulation rate of 600 to 1,500ℓ/h, respectively, when indoor air temperature was 10∼20$\^{C}$. COP of heat pump system was 3.0 to 4.0 in the heating mode. But, COP of the cooling mode was 1.3 to 2.1 at indoor temperature of 20∼35$\^{C}$. The feasibility test in the greenhouse the developed heating and cooling system was installed, showed that the heating cost of the developed system was only about 13% of that of the conventional heating system. The heating cost of the developed system was 367won/day(electric consumption 9.7㎾h/day), while that of the conventional system was 2,803won/day(oil consumption 7.7ℓ/day) at the same heating mode.

• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 1977년도 추계학술발표회 및 특별 강연초록
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• pp.192-193
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• 1977

It is certainly a difficult work to find out novel-type "practical" antibiotic from natural sources. In fact, chemically modified antibiotics, such as synthetic penicillins, cephalosporins, derivatives of tetracycline, lincomycin, rifamycin and kanamycin are most widely used in medical practice at present. However, as the possibility of success gained by chemical modification is limited, it would be the buty of our applied microbiologists to offer novel antibiotics to chemist and physician without interruption. In this lecture, some practical problems in new antibiotic research will be discussed.(중략)

• 한국농공학회지
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• 제38권3호
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• pp.70-81
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• 1996

Solutions of geotechnical engineering problems require predictions of deformation and stresses during various stages of loading. Powerful numerical methods are available to make such predictions even for complicated problems. To get accurate results, realistic stress-strain relationships of soils are dependent on a number of factors such as soil type, density, stress level and stress path. Attempts are continuously being made to develope analytical models for soils incorporating all such factors. Isotropic compression-expansion test and a series of drained conventional triaxial tests with several stress path for Baekma river sand were performed to investigate stress-strain and volume change characteristics of Lade's single work hardening model dependant on the stress path. In order to predicted of stress-strain and volumetric strain behavior were determined the values of parameters for the mode by the computer program based on the regression analysis. Predicted stress-strain behavior of triaxial compression tests and optional stress path tests for increasing confining pressure with parameters obtained conventional triaxial compression tests agreed with several test results but the prediction results for decreasing confining pressure reduced triaxial compression tests make a little difference with test results.

• Journal of the Korean Wood Science and Technology
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• 제47권4호
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• pp.442-450
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• 2019

Despite its potential as a renewable source for fuels and chemicals, lignin valorization still faces technical challenges in many aspects. Overcoming such challenges associated with the chemical recalcitrance of lignin can provide many opportunities to innovate existing and emerging biorefineries. In this work, we leveraged a biomass genetic engineering technology to produce phenolic aldehyde-rich lignin structure via downregulation of cinnamyl alcohol dehydrogenase (CAD). The structurally altered lignin obtained from the Arabidopsis thaliana CAD mutant was pyrolyzed to understand the effect of structural alteration on thermal behavior of lignin. The pyrolysis was conducted at 400 and $500^{\circ}C$ using an analytical pyrolyzer connected with GC/MS and the products were systematically analyzed. The results indicate that aldehyde-rich lignin undergoes fragmentation reaction during pyrolysis forming a considerable amount of C6 units. Also, it was speculated that highly reactive phenolic aldehydes facilitate secondary repolymerization reaction as described by the lower yield of overall phenolic compounds compared to wild type (WT) lignin. Quantum mechanical calculation clearly shows the higher electrophilicity of transgenic lignin than that of WT, which could promote both fragmentation and recondensation reactions. This work provides mechanistic insights toward biomass genetic engineering and its application to the pyrolysis allowing to establish sustainable biorefinery in the future.

• 한국환경보건학회지
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• 제39권5호
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• pp.465-473
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• 2013

Objectives: This study was undertaken to analyze Staphylococcus aureus from cultivation environments for agricultural products and to confirm antibiotic resistance and enterotoxin genes for the isolated S. aureus. Methods: A total of 648 samples were collected from apple, peach, ginseng and balloon flower farms. S. aureus was isolated from soil, agricultural water, personal hygiene elements (hands, gloves and clothes) and work utensils (boxes). Results: S. aureus was detected in a total of 25 samples and 72 strains were isolated. The resistance rate of the isolated S. aureus strains was confirmed at 33.3%, with 24 resistant strains among the total of 72. Fourteen different patterns types were found, and three pattern types (NV, OX, VA) were confirmed most frequently. As result of the detection of enterotoxin gene type, four gene types (sea: 1, sed: 4, seg: all isolated S. aureus, sei: all isolated S. aureus) were analyzed among a total of nine types. Conclusions: This study demonstrates that personal hygiene techniques should be properly managed, such as washing and sterilization before or after work, because agricultural contamination by S. aureus frequently developed through improper management.

• 농업생명과학연구
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• 제51권1호
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• pp.187-192
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• 2017

농업현장에서는 각종 작물을 재배할 때, 작물 성장을 빠르고 효과적으로 하기 위하여 일정한 크기로 성장한 묘를 밭으로 옮겨 심게 되는데, 이러한 묘 정식작업을 수행할 때 채소 정식기가 사용된다. 본 채소 정식기는 인력에 의존하고 있는 채소 정식작업의 기계화를 촉진하기 위하여 2조식의 전자동 육묘트레이 공급 및 취출된 묘를 식부호퍼에 이송할 수 있도록 개발하였다. 또한 두 가지 취출방식에 따른 묘 결주율을 비교하여 판단하였다. 핑거타입 묘 취출 시험결과 256주 묘에서 묘를 뽑아내지 못하는 경우가 17주, 상토가 부서지는 경우가 15주 등 결주율이 13.7%로 나타났으며, 포크타입 묘 취출 시험결과 200주 묘에서 묘는 100% 뽑아내지만 이송 중 낙하되는 경우가 6개 등 결주율이 4%로 나타났다. 따라서 전자동 2조식 정식의 묘 취출장치로서 포크타입이 적당한 것으로 판단되었다.

• Asian-Australasian Journal of Animal Sciences
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• 제13권2호
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• pp.220-225
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• 2000

A general household survey was carried out in the village of Sonoageng in East Java, Indonesia with the aim to assess their socio-economic status, and the crop and livestock production system prevailing in the area. Of the households interviewed (164), 52% are landless, 35% own land or have a combination of own and shared land, and 12% do not own land but have access to land by sharing. Nearly two thirds (65%) of the households raise cattle; most of them own animals, 8% reared shared animals only. The dominant crop was rice; other crops grown were soya bean, groundnut, maize and sugar cane. Nearly half (47%) the household income was derived from off-farm work (non-agricultural activities), 33% from crops, 13% from livestock (mainly cattle), and 7% from agricultural labour. Most households kept 1 to 2 animals and only 21 out of 164 households earned more than Rp. 500,000 (~250 US$) per annum from livestock. The most prevalent type of livestock production in the area could be characterized as small-scale cow/calf operation, either by landless households or those with <0.4 ha of land.

• 농업과학연구
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• 제31권1호
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• pp.26-34
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• 2004

In this study, motion characteristics and power requirement of rotary tilling according to shape of conventional rotary blade were measured. This study was performed to establish factors which needed to develop energy saving rotary blade. Starting point of edged curve of imported rotary blade was faster than that of domestic rotary blade after measuring and analyzing edged curve of rotary blade. So domestic rotary blade tills much soil than imported rotary blade. In analyzing motion of rotary blade, Rotary blade of A, D type was begun to contact at part 3. Analyzing back surface of rotary blade which contact to soil at critical $\lambda$ results in contacting at e-f part. In measuring power requirement of rotary blade, specific torque and specific work of rotary blade are $160{\sim}170kgf{\cdot}m/m^2$, $3,700kgf{\cdot}m/m^3$. It shows power requirement of rotary blade according to shape of rotary blade are very different.

• Journal of Biosystems Engineering
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• 제25권3호
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• pp.195-202
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• 2000

This study was conducted to recognize a possibility that cone index can be used as a means of evaluating the tillage workability. Cone indexes were measured every 24 hours after rainfall at the experimental plots, and the rotary and plowing operations were conducted at the same time. The workability was evaluated on a basis of three categories of good, fair and poor depending on the quality of the performed works. Although the workability was affected by many factors such as soil type, moisture content ground slope and weather condition, the duration and amount of rainfall were of most influence. Results of the study showed that a good workability was resulted from the cone indexes greater than an average of 552 kPa for rotary operations and 671 kPa for plowing operations. Fair work was obtained with cone indexes greater than an average of 331 kPa for rotary operations and 459 kPa for plowing operations. The cone indexes less than an average of 171 kPa and 149 kPa resulted in poor workabilities for rotary and plowing operations, respectively. The experimental results may provide a general guideline for evaluating the tillage workability by cone index.