• Journal of Animal Science and Technology
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• v.57 no.2
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• pp.5.1-5.8
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• 2015

Background: Korean native chicken (KNC) is a well-known breed due to its superior meat taste. This breed, however, owing to a low growth rate, has a high market price. In order to overcome this disadvantage, the National Institute of Animal Science (NIAS) in Korea developed a commercial KNC breed, named Woorimatdag version 2 (WM2), an upgraded version of the Woorimatdag (WM1) breed and the WM2 was created by crossing the KNC with meat type breeds. This study aims to discriminate between WM2 and other chicken breeds using microsatellite (MS) markers. Methods: A total of 302 individuals from eight Korean chicken populations were examined. The genetic diversity and population structure analysis were investigated using Cervus, API-CALC, STRUCTURE, PowerMarker programs. Results: Based on heterozygosity and polymorphic information content (PIC) values, 30 MS markers were initially selected from 150 markers. The identified average number of alleles (Na), expected heterozygosity, and PIC values for the WM2 samples were 7.17, 0.741, and 0.682, respectively. Additionally, the paternity of individuals was assigned with a success rate of greater than 99% using 12 markers, the best minimum number of markers. The 12 selected markers contained heterozygosity and PIC values above 0.7 and probability of identity values around zero. Using these markers, the determined probability of identity (PI), $PI_{half-sibs}$, and $PI_{sibs}$ values were 3.23E-33, 5.03E-22, and 8.61E-08, respectively. Conclusions: WM2 is well differentiated with respect to other chicken breeds based on estimated genetic distances. The results presented here will contribute to the identification of commercial WM2 chicken in the market.

• Applied Biological Chemistry
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• v.39 no.3
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• pp.206-211
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• 1996

It was generally known that over-ingestion of dietary orotic acid caused hepatic disorder by the lesion of fatty acid and cholesterol synthesis in rats. The present study was carried out to investigate the effects of dietary orotic acid on the lipid composition of serum, liver and kidney of rats. For the experiments, rats were fed with commercialized chow powder diet in the presence or absence of 1% orotic acid. The prepared diets were fed to male rats (Sprague-Dawley strain, $90{\sim}100$ g of body weight for 21 days. According to the results, orotic acid treated group showed that each concentration of serum cholesterol, triglyceride and phospholipid was significantly decreased (p<0.05). The centration of liver triglyceride was significantly decreased (p<0.05). In the presence of 1% orotic acid, the weight of liver inclosed while that of kidney decreased. The treatment of orotic acid seemed to have no effects on the phospholipid composition in liver and kidney, except the kidney lysophosphatidylcholine. In the comparison of the phospholipid composition between liver and kidney, the levels of sphingomyeline and phosphtidylethanolamine in the kidney were higher than those in the liver. Among the composition of fatty acid in kidney, the concentration of linoleic acid (18 : 2) was increased, and the concentration of arachidonic acid (20 : 4) was decreased with the addition of the orotic acid.

• Applied Biological Chemistry
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• v.51 no.1
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• pp.65-69
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• 2008

The roots of Brassica campestris ssp rapa were extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and $H_2O$. From the EtOAc fraction, three compounds were isolated through the repeated silica gel and octadecyl silica gel (ODS) column chromatography. From the results of spectroscopic data including NMR and MS, the chemical structures of the compounds were determined as caulilexin C (1), indoleacetonitrile (2) and arvelexin (3). The arvelexin (3) has been isolated from this plant for the first time. Compounds 1, 2 and 3 showed inhibitory activity on human Acyl CoA: cholesterol. transferase 1 (hACAT1) by $54.6{\pm}6.0%$, $69.2{\pm}4.7%$ and $68.6{\pm}3.7%$, and on human Acyl CoA: cholesterol transferase 2(hACAT2) by $4.8{\pm}13.4%$, $45.6{\pm}4.8%$ and $39.5{\pm}4.3%$, respectively, at 100 ${\mu}g/ml$.

• Journal of Applied Biological Chemistry
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• v.57 no.2
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• pp.179-182
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• 2014

In the present study, we investigated the effects of methanol extracts from Alpinia katsumadai Hayata against antiviral potential underlying mechanism in glucosidase inhibition. Syncytium formation in Newcastle disease virus (NDV)-infected baby hamster kidney (BHK) cell originates from the trafficking of viral glycoprotein into cell-surface. Methanol extracts inhibited not only syncytium formation, but also trafficking of glycoprotein, hemagglutinin-neuraminidase (HN), onto cell-surface. A. katsumadai extracts showed the inhibitory activities ($IC_{50}$ $25{\mu}g/mL$) against ${\alpha}$-glucosidase. These results suggested that blue chanterelle extracts inhibited the cell-surface expression of NDV-HN glycoprotein without significantly affecting HN glycoprotein synthesis in NDV-infected BHK cells.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.1
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• pp.725-731
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• 2020

This study analyzed the impact of increasing orange imports on the domestic fruit markets, focusing on the period January to May when oranges were imported and sold intensively after implementation of the Korea-US FTA. In this study, only citrus fruits that compete with U.S. oranges were limited to domestic fruits; of these, Hallabong, which is consistent with consumption of U.S. oranges, was selected as an analysis target. A dynamic recursive simulation model was established to evaluate the ex-post effects of the Korea-U.S. FTA, and to conduct mid and long-term forecasts for the Hallabong market. In addition, major policy simulations were performed on the Hallabong market to assess the effect of each scenario. The ex-post impact evaluation reveals that between December and February, Hallabong had no effect on the seasonal tariff of oranges. However, from 2012 to 2017, the actual import decreased by 21.9 billion won annually due to the TRQ, with the accumulated 6-year decrease being 131.5 billion won. Major policy simulation analysis shows that the change in the unit cost of import due to the U.S orange crop and the increase of Hallabong export will help in expanding the market, and thus effectively increase income.

• The Korean Journal of Mycology
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• v.42 no.4
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• pp.282-288
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• 2014

In this study, we attempted to isolate fungi from soybean fermented foods produced in Sunchang County and to identify Aspergillus oryzae from fungal isolates. Ten fungal isolates were identified with ${\beta}$-tubulin gene. According to the sequences of ${\beta}$-tubulin gene, ten fungal isolates were identified as A. oryzae/flavus complex. For further identification of the ten of fungal isolates, omtA gene, one gene of the aflatoxin biosynthesis gene cluster, was sequenced and the sequences were compared with those of A. oryzae and A. flavus strains from the GenBank database. In addition, identification of the ten fungal isolates was further confirmed using the PCR amplicon of norB and cypA intergenic region, in which a deletion was recognized relative to A. flavus and A. parasiticus. The amplicon size of the ten fungal isolate strains was smaller than those of A. flavus and A. parasiticus, but the same as that of the reference A. oryzae strain. These results indicated that the ten isolates should be identified as A. oryzae. The protease activity in rice koji made with 6, 13, 17, 27, 37 and 38 of strain, respectively was twice higher than that in control. The kojis made with nine of the A. oryzae isolates, respectively, did not produce aflatoxin, suggesting that the strains could possibly be used as starters for soybean products.

• Korean Journal of Medicinal Crop Science
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• v.21 no.5
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• pp.353-360
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• 2013

Korean ginseng (P. ginseng C. A. Meyer) is one of the most important medicinal plant in the world. Understanding genetic variability among the assortment of Korean ginseng is important for breeding. The aim of this study was to molecularly characterize Korean ginseng cultivar and breeding lines through the use of eight previously reported STS markers (MFGp183, MFGp130, MFGp110, UFGp74, UFGp163, MFGp108, MFGp81 and UFGp156). All STS markers produced interpretable electropherograms from 31 accessions consisting of 11 Korean ginseng cultivars and 20 breeding lines. When eight STS markers were combined, we identified to total 19 genetic patterns; in particular, nine cultivars (Chunpoong, Yunpoong, Gopoong, Gumpoong, Sunpoong, Sunone, Cheongseon, Sunhyang, Cheonryang) and 5 breeding lines (G08012, G04079, G04075, G08036, G04110) in ginseng samples can be discriminated from the others. Together with other available markers, these STS markers will contribute to the management of ginseng genetic resources and the protection of breeders' rights.

• Journal of Preventive Medicine and Public Health
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• v.22 no.3 s.27
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• pp.406-422
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• 1989

This study is aimed at developing a nutritional task of a community' as a public health activities of Su-Dong Myun, Demonstration Project area of Ewha Womans University. The content of this study is the points of nutritional diagnosis for the residents nutritional evaluation, the analysis of the related factors and the educational material with Nutrition Education-Index. Two hundreds housewives were examined during the period of Jan. 20 to Jan. 30 in 1989. 1. In terms of the housewives' age, those aged 30 to 39 were 32.5% which was the highest and educational level, graduation of primary school was 31.5% which was the highest. The housewives who had no job were 60.0% and those who participated in community activities were 56.5%. 2. In terms of the points of nutritional diagnosis, 50 to 74 points were 51.5% which was the highest and total average was $65.1{\pm}15.0$. 3. As for the points of nutritional knowledge, the average was 54.9 points, attitude was 77.4 points and eating practice was 70.8points. 4. The number of clinical symptoms of nutritional deficiency was 16.1 ones per person. 5. As for the relation which nutritional knowledge, attitude, eating practice exert on one another, nutritional knowledge has negative correlation with eating practice(-0.04) and attitude has some correlation with eating practice(0.17). 6. The variable having to do with the points of nutritional diagnosis showed the highest correlation coefficient(0.55) as the points of nutritional knowledge. 7 As for the variable having to do with the number of the symptoms of nutritional deficiency, the higher points of knowledge(-0.05) is, the higher the totai averse points of nutritional diagnisis(-0.09) is, negative correlation was appeared. 8. The result in which the variant having to do with the number of clinical symptoms of nutritional deficiency was analyzed by multiple regression analysis showed that the lack of time for preparing meals in non agricultural households made the greatest contribution (9% explained) and the households having vegetable garden made the second greatest contribution(3% explained).

• Current Research on Agriculture and Life Sciences
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• v.5
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• pp.173-184
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• 1987

Anethole, eugenol, isoeugenol, safrole and isosafrole are ingredients of refined oils which are obtained from some plants and their chemical structures are very similar. They are mainly used as a flavoring agent, food additive, dental analgesics and for many drugs. But, there is no report about their effect on the intestinal motility. The result of examining the effect, potency and mode of action of anethole, eugenol, isoeugenol, safrole and isosafrole on motility of isolated rabbit jejunum segment, are as follows : 1. Single administration of anethole, eugenol, isoeugenol, safrole and isosafrole showed the inhibition of motility of isolated rabbit jejunum segment, degree of which was various. The $pD_2$ values of isoeugenol, isosafrole, eugenol, safrole and anethole in isolated rabbit jejunum segment were 4.22, 4.18, 4.17, 4.15 and 3.82 (in the descending order of potency). 2. The contracted rabbit jejunum segment : by carbachol, pilocarpine, barium chloride and histarmine were relaxed by five essential oil. 3. The relaxed rabbit jejunum segment by anethole was not recovered by carbachol, pilocarpine, barium chloride and histamine. The relaxed rabbit jejunum segment by eugenol, isoeugenol, safrole and isosafrole were recovered by carbachol, pilocarpine and barium chloride but partially recovered by histamine. 4. Judging from the facts above, it is thought that five essential oil are inhibit the motility of isolated rabbit jejunum segment by neurotropic and musculotropic action.

• Current Research on Agriculture and Life Sciences
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• v.22
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• pp.1-12
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• 2004

Pod-edible bean or snap bean is a fairly new crop to domestic farmers but the national demand is steadily increasing in recent years along with the development of western food business and change in dietary patterns. At the same time, much efforts are being made to export it to foreign country, mainly to Japan. The amount of seeds introduced from outside is also continuously increasing along with the enlargement of area planted for the crop. Hybridization breeding for the crop has already been started to supply the cheaper and better seeds which will reduce the seed costs and foster the higher income to the farmers. In this experiment, several technologies related with the production of quality seeds are preliminary investigated. Some of the results obtained are summarized as follows; 1. Highly significant interaction was recognized between planting dates and no. of pods per plant and no. of branches but no interaction between planting dates and plant height and no. of nodes on main stem. Days to maturity was proportionally reduced to later planting dates. 2. Rate of viviparous pods and seeds was gradually increased in later planting dates but rate of germination was increased in earlier planting dates with lower germination rate in white seed coat grains than in colored seed ones. 3. Seed yield was higher in the earlier planting dates with a great deal of varietal difference. Early to mid April was considered to he the optimum planting dates for snap bean in Kyungbuk area. High correlation was recognized between seed yield and no. of pods per plant, no. of seeds per plant, and 100 seed weight. 4. Days to flowering was three and seven days longer in Cheongsong, high mountainous area than in Kunwi, somewhat prairie lowland. One hundred seed weight was also higher in Cheongsong than in Kunwi. Rate of viviparous grains, pods, and decayed seeds was higher in Cheongsong but, at the same time, the rate of germination and seed yield was also higher in Cheongsong. 5. One hundred seed weight of KLG5007 increased continuously up to 35days after flowering and decreased thereafter but that of KLG50027 increased to 40days after flowering and slowly reduced thereafter. The content of crude oil reached to maximum at 40 days after flowering and reduced thereafter. The rate of germination in Gangnangkong 1 was the highest, 89.3%, at 35 days after flowering and reduced thereafter while that in KLG50027 reached to maximum, 70.7%. at 40days after flowering and reduced thereafter. Thus, the optimum harvesting time for snap bean was considered to be 35~40days after flowering. 6. The snap bean pods at yellow bean stage easily became viviparous ones under saturated moisture conditions for 24 hours at $25{\sim}30^{\circ}C$. Therefore, it is recommended to harvest pods somewhat earlier than yellow-bean stage and let them do post maturing, especially when it is to be rained.