Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Effect of Ingested Orotic Acid on Serum, Liver and Kidney Lipid Concentration in Rats

Orotic acid 투어가 흰쥐의 혈청, 간장(肝臟) 및 신장(腎臟) 지질 농도에 미치는 영향

  • 김석환 (동아대학교 식품영양학과) ;
  • 차재영 (일본좌하대학응용생물과학과) ;
  • 조영수 (동아대학교 농화학과)
  • Published : 1996.09.30
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Abstract

It was generally known that over-ingestion of dietary orotic acid caused hepatic disorder by the lesion of fatty acid and cholesterol synthesis in rats. The present study was carried out to investigate the effects of dietary orotic acid on the lipid composition of serum, liver and kidney of rats. For the experiments, rats were fed with commercialized chow powder diet in the presence or absence of 1% orotic acid. The prepared diets were fed to male rats (Sprague-Dawley strain, $90{\sim}100$ g of body weight for 21 days. According to the results, orotic acid treated group showed that each concentration of serum cholesterol, triglyceride and phospholipid was significantly decreased (p<0.05). The centration of liver triglyceride was significantly decreased (p<0.05). In the presence of 1% orotic acid, the weight of liver inclosed while that of kidney decreased. The treatment of orotic acid seemed to have no effects on the phospholipid composition in liver and kidney, except the kidney lysophosphatidylcholine. In the comparison of the phospholipid composition between liver and kidney, the levels of sphingomyeline and phosphtidylethanolamine in the kidney were higher than those in the liver. Among the composition of fatty acid in kidney, the concentration of linoleic acid (18 : 2) was increased, and the concentration of arachidonic acid (20 : 4) was decreased with the addition of the orotic acid.

Orotic acid의 과잉 섭취는 지질대사의 이상으로 인한 간 장해를 야기시키는 것이 알려져있다. 특히 지방간 생성에 대하여 관심을 가지게 되어서 본 연구는 orotic acid에 의한 혈청, 신장(腎臟) 및 간장(肝臟)의 지질 농도에 미치는 영향에 대하여 검토하였다. 시판 분말 chow 식이에 orotic acid 1% 첨가, 무첨가한 식이를 21일간 90g 전후의 성장기 Sprague-Dawley 계(系) 웅성 쥐에 급여하였다. 그 결과 orotic acid 1% 첨가 식이에서 혈청cholesterol, triglyceride 및 phospholipid 농도는 각각 5% 수준에서 유의하게 저하 하였다. 간장(肝臟)의 triglyceride 농도는 5% 수준에서 유의하게 저하 하였다. Orotic acid 첨가 식이에서 간장(肝臟)중량은 증가한 반면 신장(腎臟)중량은 저하하였다. Orotic acid 1% 첨가한 군에서 간장(肝臟) 및 신장(腎臟)의 phospholipid조성에의 영향은 인정되지 않았으나, 신장(腎臟)의 lysophosphatidylcholine은 높은 증가율을 보였다. 간장(肝臟) phospholipid와 비교하여 신장(腎臟)에서는 sphingomyeline phosphatidylethanolamine은 유의하게 높은 분포를 나타냈다. 신장(腎臟) phospholipid의 지방산 조성중에서 linoleic acid(18:2)가 상승하고 arachidonic acid(20:4)가 감소하였다.

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