• Natural Product Sciences
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• 제12권1호
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• pp.1-7
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• 2006

We have screened the cytoprotective effect on ${\gamma}-ray$ radiation induced oxidative stress from forty one Korean plant extracts. Carpinus laxiflora (caulis), Quercus salicina (caulis), and Castanopsis cuspidata (caulis) were found to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and intracellular reactive oxygen species (ROS). As a result, extracts of three plants reduced cell death of Chinese hamster lung fibroblast (V79-4) cells induced by $H_2O_2$ treatment. In addition, these extracts protected cell death of V79-4 cells damaged by ${\gamma}-ray$ radiation. In addition, these extracts scavenged ROS generated by radiation. Taken together, the results suggest that Carpinus laxiflora, Quercus salicina, and Castanopsis cuspidata protect V79-4 cells against oxidative damage by radiation through scavenging ROS.

• The Plant Pathology Journal
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• 제30권4호
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• pp.355-366
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• 2014

In this study, resistance responses were investigated during the interaction of Botrytis fabae with two faba bean cultivars expressing different levels of resistance against this pathogen, Nubaria (resistant) and Giza 40 (susceptible). Disease severity was assessed on leaves using a rating scale from 1 to 9. Accumulation levels of reactive oxygen species (ROS), lipid peroxidation and antioxidant enzymes (superoxide dismutase, catalase and ascorbate peroxidase) were measured in leaf tissues at different times of infection. The expression profiles of two pathogenesis-related proteins (PRPs) encoded by the genes PR-1 and ${\beta}$-1,3-glucanase were also investigated using reverse transcription RT-PCR analysis. The accumulation of these defense responses was induced significantly in both cultivars upon infection with B. fabae compared with un-inoculated controls. The resistant cultivar showed weaker necrotic symptom expression, less ROS accumulation, a lower rate of lipid peroxidation and higher activity of the enzymatic ROS scavenging system compared with susceptible cultivar. Interestingly, ROS accumulated rapidly in the resistant leaf tissues and peaked during the early stages of infection, whereas accumulation was stronger and more intense in the susceptible tissues in later stages. Moreover, the response of the resistant cultivar to infection was earlier and stronger, exhibiting high transcript accumulation of the PR genes. These results indicated that the induction of oxidant/antioxidant responses and the accumulation of PRPs are part of the faba bean defense mechanism against the necrotrophic fungus B. fabae with a different intensity and timing of induction, depending on the resistance levels.

• The Plant Pathology Journal
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• 제33권5호
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• pp.458-466
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• 2017

Xanthomonas oryzae pv. oryzae (Xoo), the causative agent of bacterial blight, is a major threat to rice productivity. Here, we performed RNA-Seq based transcriptomic analysis of Xoo transcripts isolated under in planta growth (on both susceptible and resistant hosts) and in vitro culture conditions. Our in planta extraction method resulted in successful enrichment of Xoo cells and provided RNA samples of high quality. A total of 4,619 differentially expressed genes were identified between in planta and in vitro growth conditions. The majority of the differentially expressed genes identified under in planta growth conditions were related to the nutrient transport, protease activity, stress tolerance, and pathogenicity. Among them, over 1,300 differentially expressed genes were determined to be secretory, including 184 putative type III effectors that may be involved in Xoo pathogenicity. Expression pattern of some of these identified genes were further validated by semi-quantitative RT-PCR. Taken together, these results provide a transcriptome overview of Xoo under in planta and in vitro growth conditions with a focus on its pathogenic processes, deepening our understanding of the behavior and pathogenicity of Xoo.

• 한국잡초학회지
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• 제4권1호
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• pp.96-106
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• 1984

Changes in weed floras and development of plant resistance to herbicides seemed to be closely related with increased and repeated use of herbicides. Herbicide use increased from 5% of the total consumption of pesticide in 1950 to 45% in 1976 in world basis. About 200 herbicides have been introduced to agriculture so as to control about 206 weed species which have been recorded important to human beings. In Korea, there was about 351 times in increased use of herbicides from 1966 to 1982. Interspecific selection by herbicide is mainly responsible for changes in weed floras and resulted in varying tolerance or susceptibility to herbicides, together with the changes of agricultural practices. The present trend toward continuous cereal cultivation throughout world will lead to type of changes in weed floras favorable to therophyte which can survive under unfavorable conditions as seeds rather than the types of geophyte which can survive unfavorable seasons as buds placed below soil surface. However, geophyte such as Sagitaria pygmaea, and Scirpus jurtcoides, and Cyperus rotundus and Cynodon dactylon in temperate warm climate become severe paddy weeds, presumably because of the removal of annual weeds by herbicides. Since differential tolerance to 2,4-D was firstly reported in Agrostis stolofera, about 30 species of weeds in 18 genera are presently known to have developed resistance to triazine herbicides. Resistance of weed biotypes to triazine herbicide is not mainly due to limited absorption and translocation or to the difference in metabolism, but is the result of biochemical changes at the site of metabolic activity, such as a loss of herbicide affinity for triazine binding site in the photosystem II complex of the chloroplast membrane. Genetical study showed that plastid resistance to triazine was wholly inherited through cytoplasmic DNA in the case of Brassica campestris. Plant tissue culture method can be utilized as an alternate mean of herbicide screening and development of resistance variants to herbicides as suggested by Chaleff and Parsons. In this purpose, one should be certain that the primary target process is operational in cell culture. Further, there are a variety of obstacles in doing this type of research, particularly development of resistance source and it's regeneration because cultured cells and whole plants represent different developmental state.

• 한국균학회지
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• 제30권2호
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• pp.131-135
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• 2002

표고버섯 균사체가 항당뇨 효과에 미치는 영향을 검토하기 위해 당뇨가 유발된 흰쥐에 표고버섯 균사체을 7일간 급여시킨 후 혈장 glucose, total cholesterol, triglyceride 및 ALT, AST 활성을 관찰하였고, 표고버섯 균사체 생장의 최적 배양 조건을 조사하였다. 균사체 생장을 위한 최적의 pH와 온도는 5와 $30^{\circ}C$로 나타났으며, 발효조배양시에는 최대 균사체 생장(8.13g/l)이 진탕배양일 때보다 6일 단축되었다. Hypoglycemic 효과에서 혈장 glucose는 당뇨 유발군에서 표고버섯 균사체 급여군이 대조군보다 23.0%가 유의하게 감소하였으며, 혈장 total cholesterol과 triglyceride는 20.3%와 27.1%로 낮게 나타났다. 또한 혈장 ALT와 AST 활성은 당뇨 대조군에 비해 표고버섯 균사체 급여군이 29.6%와 34.3% 낮은 활성을 보였다.

• 한국식품저장유통학회지
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• 제15권6호
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• pp.859-863
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• 2008

포도씨의 품종에 따른 총 페놀 함량과 proanthocyanidin의 함량과 항산화 활성을 알아보았다. 총 페놀 함량은 Folin-Ciocalteu법으로 측정하였고, $16.71\;{\sim}\;28.60\;mg$/100 g의 함량을 나타내었다. Proanthocyanidin 함량은 vanillin sulfuric acid법으로 측정하였고, $18.36\;{\sim}\;55.30\;mg$/100 g의 함량을 나타내었다. 품종 중에서 자옥 품종이 가장 높은 페놀 함량과 proanthocyanidin 함량을 나타내었다. 항산화 활성은 자옥 품종이 역시 가장 강한 항산화 활성을 나타내었다. 총 페놀 함량과 proanthocyanidin 함량은 항산화 활성과 높은 상관성을 보였다 (>0.92). 따라서 포도씨 추출물은 항산화 건강 기능성 식품의 소재로서 유용하게 활용될 수 있을 것으로 기대된다.

• 한국식품저장유통학회지
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• 제15권6호
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• pp.878-883
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• 2008

항균력이 탁월한 매실추출물로부터 항균활성물질을 column chromatography를 이용하여 순수하게 분리하고, nuclear magnetic resonance (NMR) spectrophtometer 측정기에 의하여 항균활성물질의 화학구조를 다음과 같이 분리 동정하였다. 즉, 매실추출물을 상온에서 메탄올에 현탁한 후, ethyl ether, ethyl acetate, n-butanol로 각각 분배 추출하여 각 용매의 수용성 추출물을 획득하였다. 각 용매의 수용성 추출물을 loading한 후, silica gel(70-230 mesh, Merck) column chromatography하여 소획분을 얻었다. 분리된 분획 중 항균력이 있는 소획분을 Sephadex LH 20을 충진하고 column chromatography하여 각 항균분획물질을 분리하였다. column chromatography에서 분리한 각 항균분획물질(Compound A, Compound B, Compound C)의 구조분석을 위해 $^{1}H$ 및 $^{13}C$ NMR 스펙트럼을 실온에서 측정하였다. NMR은 Bruker DRX500 (500MHz)을 이용하여 측정하였으며, 용매와 기준물질은 $CDCl_3$와 TMS를 각각 사용하였다. 이와같이 분리한 각 항균분획물질을 NMR 분석법으로 동정한 결과, isoeugenol, nomilin 및 $\beta$-sitosterol 등으로 확인되었다.

• 한국약용작물학회지
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• 제10권3호
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• pp.167-170
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• 2002

1998년 8월부터 11월까지 선학초(仙鶴草) 전초(全草) 추출액(抽出液)을 처리수준 0%, 0.02%, 0.04%, 0.06% 및 0.08%로 하여 흰쥐(Rat)에 경구투여(經口投與)하여 성장(成長) 및 생리활성상태(生理活性狀態)를 조사한 결과는 다음과 같다. 1. 선학초(仙鶴草) 전초(全草) 추출액(抽出液)을 흰쥐에 농도별(濃度別)로 투여한 결과 선학초(仙鶴草) 추출액(抽出液) 급여시(給與時) 증체양(增體量)은 무급(無給) 여구(與區) 대비(對比) 0.02% 급여구(給與區)가 353.2g으로 5% 성장하였으나 0.04% 급여구(給與區)에서는 5% 감소하는 경향을 보였다. 2. 사두효솔면(飼枓效率面)에서는 무급여구(無給與區)와 0.02% 급여구(給與區)에서는 0.14로 비슷한 경향을 보였으나 다른 수준에서는 7%정도 낮아지는 경향을 보였다. 3. 백혈구(白血球) 수(數)는 무급여구(無給與區)에 비해 0.02% 급여구(給與區)를 제외한 전(全) 급여구(給與區)에서 급여수준(給與水準)이 증가할 수록 백혈구(白血球) 수(數)가 증가하는 경향을 보였다. 4. 혈소판(血小板) 수(數)는 0.06% 급여구(給與區)에서 무급여구(無給與區)에 비해 20% 증가하는 경향을 나타냈다.

• Nutrition Research and Practice
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• 제9권1호
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• pp.71-78
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• 2015

BACKGROUND/OBJECTIVES: The purposes of this study were to compare total energy expenditure (including PAL and RMR) of Korean farmers between the farming season and off farming season and to assess the accuracy of estimated energy requirement (EER) prediction equation reported in KDRIs. SUBJECTS/METHODS: Subjects were 72 Korean farmers (males 23, females 49) aged 30-64 years. Total energy expenditure was calculated by multiplying measured RMR by PAL. EER was calculated by using the prediction equation suggested in KDRIs 2010. RESULTS: The physical activity level (PAL) was significantly higher (P < 0.05) in the farming season (male $1.77{\pm}0.22$, female $1.69{\pm}0.24$) than the off farming season (male $1.53{\pm}0.32$, female $1.52{\pm}0.19$). But resting metabolic rate was significantly higher (P < 0.05) in the off farming season (male $1,890{\pm}233kcal/day$, female $1,446{\pm}140kcal/day$) compared to the farming season (male $1,727{\pm}163kcal/day$, female $1,356{\pm}164kcal/day$). TEE ($2,304{\pm}497kcal/day$) of females was significantly higher in the farming season than that ($2,183{\pm}389kcal/day$) of the off farming season, but in males, there was no significant difference between two seasons in TEE. On the other hand, EER of male and female ($2,825{\pm}354kcal/day$ and $2,115{\pm}293kcal/day$) of the farming season was significantly higher (P < 0.05) than those ($2,562{\pm}339kcal/day$ and $1,994{\pm}224kcal/day$) of the off farming season. CONCLUSIONS: This study indicates that there is a significant difference in PAL and TEE of farmers between farming and off farming seasons. And EER prediction equation proposed by KDRI 2010 underestimated TEE, thus EER prediction equation for farmers should be reviewed.

• 농촌계획
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• 제18권4호
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• pp.117-127
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• 2012

The demand of rural tourism industry has increased among the urbanites in South Korea, in due to the increase of leisure activity and the emergence of ageing society. Rural amenity resources are gaining various interests, in the value creation and promotion of tourism. In this study, the propensities of city dwellers were separated by life-style classification, and each affinity to the rural amenity resources was examined in accordance with the separation. A questionnaire survey of urbanites in the southern area of Gyeonggi-do, the most populous province in South Korea, was conducted to analyze the preference of city dwellers about rural amenity resource and life-style of themselves. For statistical verification, $IBM^{(R)}$ $SPSS^{(R)}$ Statistics 20 software was used for frequency, reliability, factor and multiple regression analysis of this research. The results of the statistical analyses found a noticeable characteristic in life-style classification. The affinities of urbanites can be classified into four congregations of life-style factors in this statistical model. Each congregation of the factors was named as 'Self-development-oriented', 'Leisure-oriented', 'Achievement-oriented', and 'Culture-oriented' life-style, to represent the characteristics for convenience' sake. Among these styles, only 'Self-development-oriented' and 'Achievement-oriented' showed the positive correlation with rural amenity resources in the multiple regression analysis. In addition, the rural amenity resources were also analyzed in accordance with the life-styles classification of urbanites. City dwellers showed the highest interest to the 'natural resource management facility resource' in natural resources, the 'traditional heritage resource' in cultural resources, and the 'community resource' in social resources. Meanwhile, they showed less interest to 'agricultural and scenery resources' in natural resources, 'specialty production resource' in cultural resources, and 'cooperative farming' in social resources. These characteristics can be constructed as meaning that the urbanites who concern self-development and achievement of their lives have high interest in rural amenity resources, and the main interest of them is not 'return-to-the-farm'(歸農) but 'return-to-the-home'(歸村).