• 동의신경정신과학회지
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• 제10권2호
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• pp.71-83
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• 1999

This study was to investigate the effects of SUNGSIMJIHWANGTANG(SSJHT) on the blood and brain tissues of aged rats. For the experiment, the aged rats were divided into three groups : Non treated group(NC), distilled water fed group(PC), SSJHT fed group(SSJHT). Each group was fed for ten days before administration of scopolamine. Then, we injected scopolamine intraperitoneally to PC and SSJHT group for 5 days. We observed the changes of their blood cell(WBC, RBC, platelet), blood serum(BUN, creatinine, glucose, uric acid), erythrocyte hemolysis, and the activities of cholinesterase and measured the amounts of malondialdehyde in the serum, catalase, and SOD in the brain tissue. The main results of this investigation are as follows. 1. In respect of the number of WBC, SSJHT group exhibited significant increase in comparison with PC. 2. In respect of the amount of creatinine and uric acid in the blood serum, SSJHT group exhibited significant decrease in comparison with PC. 3. In respect of erythrocyte hemolysis, SSJHT group exhibited significant suppression in comparison with PC. 4. In respect of the activity of cholinesterase in the serum, SSJHT group exhibited significant activation in comparison with PC. 5. In respect of the amounts of malondialdehyde in the serum, SSJHT group exhibited significant decrease in comparison with PC. 6. In respect of the activity of catalase in brain tissue, SSJHT group didn't exhibit significant change in comparison with PC. 7. In respect of the activity of SOD in brain tissue, SSJHT group exhibited significant activation in comparison with PC. As a result of this study, SSJHT is expected to have antiaging effect by suppressing the formation of free radicals, the accumulation of antioxidants and further study needs to be carried on about SSJHT.

• 동의신경정신과학회지
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• 제9권2호
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• pp.71-85
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• 1998

This study was to investigate the effects of GONGCHENGWHAN(GCW) on the blood and brain tissues of aged rats. For the experiment, the aged rats were divided into three groups : Non treated group (NC), distilled water feeding group(PC), GCW feeding group(GCW).Each group was treated as the procedure for ten days before administration of scopolamine. After this treating, we injected scopolamine to Pc and GCW group their blood cell(WBc, RBC, platelet), blood serum (BUN, creatinine, glucose, uric acid), erythrocyte hemolysis, and the activities of cholinesterase as well as measured the amounts malondialdehysde in the blood serum, catalase, and SOD in the brain tissue.The main results of this investigation are as follows.1. In respect of the number of WBC, GCW group exhibited the administrated incerase in comparison with PC. In respect of the number of RBC· and platelet, however, the changes was not administrated.2. In respect of the number of BUN, creatinine, glucose and uric and the blood, GCW group exhibited the administrated decrease in comparison with PC.3. In respect of erythrocyte hemolysis, GCW group exhibited the administrated repression in comparison with PC.4. In respect of the activity of cholinestease in the blood serum, GCW group exhibited the administrated improvement in comparison with PC.5. In respect of the amounts of malondialdehyde in the blood serum, GCW group exhibited the administrated dexrease in comparison with PC.6. In respect of the activity of catalase in brain tissue, GCW group didn't exhibit the administrated change in comparison with PC7. In respect of the activity of SOD in brain tissue, GCW group exhibited the administrated improvement in comparison with PC.Results of this study indicates that GCW improves the activities of cholinesterase and SOD, but represses the formation of the free radical and the accumulation of the antioxidant materials such as MDA. It implies that GCW has some effects on antiaging.

• Journal of Microbiology and Biotechnology
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• 제28권3호
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• pp.357-366
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• 2018

Periodontitis, an infective disease caused by oral pathogens and the intrinsic aging process, results in the destruction of periodontal tissues and the loss of alveolar bone. This study investigated whether Boesenbergia pandurata extract (BPE) standardized with panduratin A exerted anti-periodontitis effects, using an aging model representative of naturally occurring periodontitis. In aged rats, the oral administration of BPE ($200mg{\cdot}kg^{-1}{\cdot}day^{-1}$) for 8 weeks significantly reduced the mRNA and protein expression of $interleukin-1{\beta}$, nuclear factor-kappa B, matrix metalloproteinase (MMP)-2, and MMP-8 in gingival tissues (p < 0.01). In alveolar bone, histological analysis with staining and micro-computed tomography revealed the attenuation of alveolar bone resorption in the BPE-treated aged group, which led to a significant reduction in the mRNA and protein expression of nuclear factor of activated T-cells c1 (NFATc1), c-Fos, tartrate-resistant acid phosphatase, and cathepsin K (p < 0.01). BPE not only increased the expression of osteoblast differentiation markers, such as alkaline phosphate, and collagen type I (COL1A1), but also increased the ratio of osteoprotegerin to RANKL. Collectively, the results strongly suggested that BPE is a natural resource for the prevention or treatment of periodontal diseases.

• 한국체육학회지인문사회과학편
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• 제55권6호
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• pp.691-700
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• 2016

본 연구의 목적은 장기간의 지구성 운동 또는 저항성 운동이 중년 흰쥐의 골격근 내 단백질 합성과 분해에 미치는 영향을 규명하는 것이다. 50 주령의 male Wistar rat 30 마리를 이용하여 3집단(좌업, 지구성 운동집단, 저항성 운동집단)으로 무선배정한 후 12 주간 처치를 실시하였다. 연구결과 12주간의 저항성운동은 중년 쥐의 족저근 내 Akt/mTOR 신호전달체계를 활성화시켰고, FoxO1/MuRF1 단백질 발현을 저해하였다. 지구성 운동은 mTOR 신호전달체계에는 영향을 미치지 않았으나 FoxO1/MuRF1 단백질 발현을 저해하였고, AMPK/PGC-1α 발현을 증가시켰다. 요약하면 12주간의 운동트레이닝은 중년 흰쥐의 골격근 내 단백질 동화/이화반응에 긍정적인 영향을 미쳤으며, 지구성 운동은 근단백질 이화반응의 저해를 통해서 노화근육의 단백질 균형에 영향을 미치는 것으로 나타났다. 이러한 결과를 통해서 근 손실에 따른 근 기능의 저하가 가시화되기 시작하는 중년을 대상으로 실시되는 운동처방 시 근 감소증 예방 및 치료를 위해 저항성 운동만이 아니라 중강도 이상의 지구성 운동도 유효할 것으로 생각된다.

• Journal of Animal Science and Technology
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• 제66권1호
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• pp.178-203
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• 2024

Constipation, which refers to difficulties in defecation and infrequent bowel movement in emptying the gastrointestinal system that ultimately produces hardened fecal matters, is a health concern in livestock and aging animals. The present study aimed to evaluate the potential effects of dairy-isolated lactic acid bacteria (LAB) strains to alleviate constipation as an alternative therapeutic intervention for constipation treatment in the aging model. Rats were aged via daily subcutaneous injection of D-galactose (600 mg/body weight [kg]), prior to induction of constipation via oral administration of loperamide hydrochloride (5 mg/body weight [kg]). LAB strains (L. fermentum USM 4189 or L. plantarum USM 4187) were administered daily via oral gavage (1 × 10 Log CFU/day) while the control group received sterile saline. Aged rats as shown with shorter telomere lengths exhibited increased fecal bulk and soften fecal upon administration of LAB strains amid constipation as observed using the Bristol Stool Chart, accompanied by a higher fecal moisture content as compared to the control (p < 0.05). Fecal water-soluble metabolite profiles showed a reduced concentration of threonine upon administration of LAB strains compared to the control (p < 0.05). Histopathological analysis also showed that the administration of LAB strains contributed to a higher colonic goblet cell count as compared to the control (p < 0.05). The present study illustrates the potential of dairy-sourced LAB strains as probiotics to ameliorate the adverse effect of constipation amid aging, and as a potential dietary intervention strategy for dairy foods including yogurt and cheese.

• 대한약침학회지
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• 제16권1호
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• pp.21-29
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• 2013

Objective: The study was carried out to evaluate the hepatoprotective and antioxidant potential of Ziziphus mucronata (ZM) fruit extract. Methods: The different types of fruit extract were prepared by soaking the dry powdered fruit in different solvents followed by rotary evaporation. Each extract was tested for its phenol content and antioxidant activities. An in vivo study was performed in Sprague-Dawley (SD) rats. Thirty adult male SD rats (aged 21 weeks) were divided into six groups of five rats each and treated as follows: The normal control (NC) received distilled water while the dimethoate control (DC) received 6 mg/kg.bw.day-1 dimethoate dissolved in distilled water. The experimental groups E1, E2, E3, and E0 received dimethoate (6 mg/kg.bw) + ZMFM (100 mg/kg.bw-1), dimethoate (6 mg/kg.bw) + ZMFM (200 mg/kg.bw-1), dimethoate (6 mg/kg.bw) + ZMFM (300 mg/kg.bw-1), and ZMFM (300 mg/kg.bw-1) only. Both the normal control and the dimethoate control groups were used to compare the results. After 90 days, rats were sacrificed, blood was collected for biochemical assays, and livers were harvested for histological study. Results: High phenol content was estimated, and 2, 2-diphenyl-1-picryl hydrazyl radical (DPPH) spectrophotometric, thin layer chromatography (TLC) and 2, 2-Azobis-3-ethyl benzothiazoline-6-sulphonic acid (ABTS) assays showed a high antioxidant activity among the extracts. The preventive effects observed in the E1, E2 and E3 groups proved that the extract could prevent dimethoate toxicity by maintaining normal reduced glutathione (GSH), vitamin C and E, superoxide dismutase, catalase, cholineasterase and lipid profiles. The preventive effect was observed to be dose dependent. The EO group showed no extract-induced toxicity. Histological observations agreed with the results obtained in the biochemical studies. Conclusion: The study demonstrated that ZM methanol fruit extract is capable of attenuating dimethoate-induced toxicity because of its high antioxidant activity.

• The Korean Journal of Physiology and Pharmacology
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• 제4권3호
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• pp.169-175
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• 2000

To learn the developmental changes in intrinsic electrophysiological properties of the second order taste neurons, whole cell recordings from the developing nucleus of the solitary tract neurons were done in brainstem slices of postnatal rats. Rats aged from postnatal 0 to 21 days (P0-P21) were used, being divided into 3 age groups: postnatal first week (P0-P7 days), second week (P8-P14 days), and third week (P15-P21 days). Slices containing gustatory NTS were cut horizontally in the thickness of $300\;{\mu}m.$ Whole cell recordings were obtained from neurons in response to a series of hyperpolarizing and depolarizing current pulses. The intrinsic electrophysiological properties of the rostral NTS (rNTS) neurons were compared among the age groups. Depolarizing current pulses evoked a train of action potentials in all neurons of all age groups. The resting membrane potential and input resistance of the neurons did not show any significant differences during the postnatal 3 weeks. The time constant, however, decreased during the development. Duration of action potential measured at half maximum amplitude was longer in younger age groups. Both the maximum rate of rise and the maximum rate of fall in the action potential increased during the first 3 weeks postnatal. Electrophysiologically more than half neurons were type III. In summary, it is suggested that developmental changes in electrophysiological properties in rNTS occur during the first three weeks in rats.

• 대한가정학회지
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• 제8권1호
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• pp.124-145
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• 1970

The author carried out this study to research the biological value of the protein fermented wheat bran. This study mainly devided into two parts. First part includes seven subgroups composed of five rats and second part fourteen subgroups composed of six rats each. In the first part of this study forty weanling male rats, aged 40${\pm}$5 days were fed by fermented wheat bran diet for seven weeks. In second part of this study 90 male weanling rats were fed by rice and soybean diet supplemented by fermented wheat bran. In the first part of this study food consumption rate of fermented group showed higher than standard group but the former group is lower than the later in the body weight gains. In the second part of this study, rice groups showed same trend of the result of the first part, but soybean groups did not go along with the result of rice group. In regard to body nitrogen retention rate, fermented wheat diet group showed higher than the standard group in the first part of this study. In the second part of this study rice diet groups revealed same result as that of the first part of this study but soybean diet did not give the same result in this respect. There was no significant difference in organ weight in all experimental groups but devided by 100 gram of body weight, the reverse is true in compare with body weight gains. Hematological investigation did not show the significance in both experimental and standard groups of the first and the second studies. It is conclusion of this study that rice which is inferior to soybean in protein quality, increases the rate of supplementary effect by fermented wheat bran in all respects of this study.

• 대한한방내과학회지
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• 제25권3호
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• pp.473-481
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• 2004

Objective : In this study old BCAO rats were observed for effects of 'Dea-Hwang' on brain ischema injury, because risk of stroke increases with age. Method : The brain ischema injury was induced by temporarily closing carotids on both sides in a low blood pressuer state and Dea-Hwang was administered orally to 18 month-old BCAO rats. Results and Conclusions : The ischemically damaged Hippocampus and c-fos and c-jun expression were analyzed by immunohistochemical staining and results are summarized as follows: 1. The c-fos expression after inducing a brain ischema injury in the hippocampus was more inhibited in the dosed group than in the control group. 2. The normalized optical density of c-fos expression was more reduced in the CA1, CA2, and DG areas of the dosed group than in those of the control group. 3. The c-jun expression after inducing brain ischema injury in the hippocampus was more inhibited in the dosed group than in the control group. 4. The normalized optical density of c-jun expression was more reduced in the CAI area of the dosed group than in that of the control group.

• Asian-Australasian Journal of Animal Sciences
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• 제21권6호
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• pp.883-889
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• 2008

Lipid peroxidation (LPO) has been identified as an important component of atherosclerosis. In this study, the effects of supplementation with cholesterol (0.5%), olive oil (5%) and vitamin E (0.05%) on erythrocyte glutathione (GSH), plasma malondialdehyde (MDA), total cholesterol, HDL-LDL cholesterol and triacylglycerol, brain and liver MDA and GSH concentrations of rats were investigated. A total of 50 Sprague-Dawley male rats aged 6 months, and of equal body weight were used and fed a standard ration ad libitum. Animals were housed in the University of Selcuk, Veterinary Faculty Experimental Animals Unit. The experiment lasted 60 days and there were five experimental groups as follows: 1. Control, 2. Cholesterol (0.5%), 3. Olive oil (5%), 4. Cholesterol plus vitamin E (0.05%), 5. Olive oil plus vitamin E (0.05%). At the end of the experiment, blood samples were taken by cardiac puncture and erythrocyte GSH, plasma MDA, cholesterol, HDL-LDL cholesterol, triacylglycerol and also GSH and MDA concentrations in brain and liver tissue of rats were spectrophotometrically determined. Supplementation of olive oil and cholesterol into rat diets (groups 2 and 3) caused significant differences in lipid parameters; HDL cholesterol concentrations were increased in the olive oil group and LDL cholesterol was lower than in the cholesterol fed group. Moreover, these decreases in LDL and triacylglycerol concentrations were more significant with vitamin E supplementation. The high plasma MDA concentrations showed that lipid peroxidation occurred in the olive oil group and the highest brain MDA concentrations were determined also in the olive oil group. These findings suggest that vitamin E addition may decrease the sensitivities of several oils to oxidation and that monounsaturated fatty acids in olive oil may decrease the incidence of atherosclerosis by regulating blood lipid profiles.