• 한국생물공학회:학술대회논문집
• /
• 2000.04a
• /
• pp.89-91
• /
• 2000

Polynuclear aromatic hydrocarbon (PAH) compounds are highly carcinogenic chemicals and common groundwater contaminants that are observed to persist in soils. The adherence and slow release of PAHs in soil is an obstacle to remediation and complicates the assessment of cleanup standards and risks. Biological degradation of PAHs in soil has been an area of active research because biological treatment may be less costly than conventional pumping technologies or excavation and thermal treatment. Biological degradation also offers the advantage to transform PAHs into non-toxic products such as biomass and carbon dioxide. Ample evidence exists for aerobic biodegradation of PAHs and many bacteria capable of degrading PAHs have been isolated and characterized. However, the microbial degradation of PAHs in sediments is impaired due to the anaerobic conditions that result from the typically high oxygen demand of the organic material present in the soil, the low solubility of oxygen in water, and the slow mass transfer of oxygen from overlying water to the soil environment. For these reasons, anaerobic microbial degradation technologies could help alleviate sediment PAH contamination and offer significant advantages for cost-efficient in-situ treatment. But very little is known about the potential for anaerobic degradation of PAHs in field soils. The objectives of this research were to assess: (1) the potential for biodegradation of PAH in field aged soils under denitrification conditions, (2) to assess the potential for biodegradation of naphthalene in soil microcosms under denitrifying conditions, and (3) to assess for the existence of microorganisms in field sediments capable of degrading naphthalene via denitrification. Two kinds of soils were used in this research: Harbor Point sediment (HPS-2) and Milwaukee Harbor sediment (MHS). Results presented in this seminar indicate possible degradation of PAHs in soil under denitrifying conditions. During the two months of anaerobic degradation, total PAH removal was modest probably due to both the low availability of the PAHs and competition with other more easily degradable sources of carbon in the sediments. For both Harbor Point sediment (HPS-2) and Milwaukee Harbor sediment (MHS), PAH reduction was confined to 3- and 4-ring PAHs. Comparing PAH reductions during two months of aerobic and anaerobic biotreatment of MHS, it was found that extent of PAHreduction for anaerobic treatment was compatible with that for aerobic treatment. Interestingly, removal of PAHs from sediment particle classes (by size and density) followed similar trends for aerobic and anaerobic treatment of MHS. The majority of the PAHs removed during biotreatment came from the clay/silt fraction. In an earlier study it was shown that PAHs associated with the clay/silt fraction in MHS were more available than PAHs associated with coal-derived fraction. Therefore, although total PAH reductions were small, the removal of PAHs from the more easily available sediment fraction (clay/silt) may result in a significant environmental benefit owing to a reduction in total PAH bioavailability. By using naphthalene as a model PAH compound, biodegradation of naphthalene under denitrifying condition was assessed in microcosms containing MHS. Naphthalene spiked into MHS was degraded below detection limit within 20 days with the accompanying reduction of nitrate. With repeated addition of naphthalene and nitrate, naphthalene degradation under nitrate reducing conditions was stable over one month. Nitrite, one of the intermediates of denitrification was detected during the incubation. Also the denitrification activity of the enrichment culture from MHS slurries was verified by monitoring the production of nitrogen gas in solid fluorescence denitrification medium. Microorganisms capable of degrading naphthalene via denitrification were isolated from this enrichment culture.

• Journal of Korean Society on Water Environment
• /
• v.25 no.2
• /
• pp.322-328
• /
• 2009

The advantage of simultaneous nitrification and denitrification (SND) is to reduce requirement of oxygen as well as tank volume. The fludized media was used in the oxic (aerobic) tank of Membrane-BNR to enhance the efficiency of SND. Nowadays, the interest of applying membrane to the wastewater treatment plant has been increased, which is proved by a lot of research published about the MBR. The Membrane-BNR, consisted of total 5 reactors might be called the compact process by using the fludized media and having short HRT of 6.5 hr. It could attain the further removal of not only the organics but also nutrients such as T-N and T-P. The mode A and B were identified with or without the step feed of influent. The mode A was classified with 3 modes according to the different DO concentration in the fludized media aerobic reactor, and the mode B with step feed was operated with the optimum DO condition. The step-feed was capable of improving TN removal efficiency under the domestic wastewater with the low ratio C/N. On the other hand, the efficiency of SND with the 1.0~1.5 mg/L DO in the oxic media tank was better than the one with below 1.0 mg/L, on which the nitrification did not happen enough, and with above 3.5 mg/L, on which the reduction of anoxic area in the tank happened. It means that the profitable nitrification should be performed prior to the denitrification step. The removal efficiency of nitrogen by SND was about 20% among of total denitrified nitrogen. And some organic carbon consumed could be reduced by the endogeneous denitrification.

• Journal of Environmental Science International
• /
• v.8 no.5
• /
• pp.617-623
• /
• 1999

This study was carried out to obtain the optimal operating parameter on organic matters and nutrient removal of mixed wastewater which was composed of sewage and stable wastewater using SBR. A laboratory scale SBR was operated with An/Ae(Anaerobic/Aerobic) ratio of 3/3, 2/4 and 4/2(3.5/2.5) at organic loading rate of 0.14 to 0.27 kgBOD/$m^3$/d. TCOD/SCOD ratio of mixed wastewater was 3, so the important operating factor depended upon the resolving the particulate parts of wastewater. Conclusions of this study were as follows: 1) For mixed wastewater, BOD and COD removal efficiencies were 93-96% and 85-89%, respectively. It was not related to each organic loading rate, whereas depended on An/Ae ratio. During Anarobic period, the amount of SCOD consumption was very little, because ICOD in influent was converted to SCOD by hydrolysis of insoluble matter. 2) T-N removal efficiencies of mixed wastewater were 55-62% for Exp. 1, 66-76% for Exp. 2, and 67-81% for Exp. 3, respectively. It was found that nitrification rate was increased according to organic concentration in influent increased. Therefore, the nitrification rate seemed to be achieved by heterotrophs. During anoxic period, denitrification rate depended on SCOD concentration in aerobic period and thus, was not resulted by endogenous denitrification. However, the amount of denitrification during anaerobic period were 3.5-14.1 mg/cycle, and that of BOD consumed were 10-40 mg/cycle. 3) For P removal of mixed wastewater, EBPR appeared only Mode 3($3^＊$). It was found that the time in which ICOD was converted to VFA should be sufficient. For mode 3 in each Exp., P removal efficiencies were 74, 87, and 81%, respectively. But for 45-48 of COD/TP ratio in influent, P concentration in effluent was over 1 mg/L. It was caused to a large amount of ICOD in influent. However, as P concnetration in influent was increased, the amounts of P release and uptake were increased linearly.

• Journal of Korean Society of Water and Wastewater
• /
• v.20 no.6
• /
• pp.875-883
• /
• 2006

The carbon and nitrogen removal process using PVA-gel (Polyvinyl Alcohol) immobilized microorganisms was studied. The process has been operated under diverse process conditions for 12 months. The process consists of denitrification with internal recycle of 300%, nitrification, aerobic digestion reactors and settling tank. Nitrificatoin and nitrogen removal efficiency according to gel packing ratio and temperature were studied. Yield for Heterotrophs ($Y_H$), decay coefficient for Heterotrophs($b_H$) in aerobic digestion reactor were determined to seize sludge reduction mechanism and compared with typical data of activated sludge process. Then SRT in aerobic digestion reactor was determined on an experimental basis and sludge reduction efficiency was calculated. The process was implemented successfully with sludge reduction efficiency of 92.0~98.5% on a basis of biomass.

• Journal of Korean Society on Water Environment
• /
• v.22 no.3
• /
• pp.566-571
• /
• 2006

A sequencing batch biofilm reactor (SBBR) operated with a cycle of anaerobic - aerobic - anoxic - aerobic has been evaluated for the nutrient removal characteristics. The sponge-like moving media was filled to about 10% of reactor volume. The sewage was the major substrate while external synthetic carbon substrate was added to the anoxic stage to enhance the nitrogen removal. The operational results indicated that maximum T-N and T-P removal efficiencies were 97% and 94%, respectively were achieved, while COD removal of 92%. The observations of significant nitrogen removal in the first aerobic stage indicated that nitrogen removal behaviour in this SBBR was different to conventional SBR. Although the reasons for aerobic nitrogen removal has speculated to either simultaneous nitrification and denitrification or anoxic denitrification inside of the media, further researches are required to confirm the observation. The specific oxygen uptake rate (SOUR) test with biofilm and suspended growth sludge indicated that biofilm in SBBR played a major role to remove substrates.

• Environmental Engineering Research
• /
• v.19 no.1
• /
• pp.75-81
• /
• 2014

Methanotrophic denitrification under a non-aerated condition (without external supply of oxygen or air) was investigated in a bioreactor coupled with a membrane diffuser. Batch experiment demonstrated that both methane consumption and nitrogen production rates were not high in the absence of oxygen, but most of the nitrate was reduced into $N_2$ with 88% recovery efficiency. The methane utilized for nitrate reduction was determined at 1.63 mmol $CH_4$/mmol $NO_3{^-}$-N, which was 2.6 times higher than the theoretical value. In spite of no oxygen supply, methanotrophic denitrification was well performed in the bioreactor, due to enhanced mass transfer of the methane by the membrane diffuser and utilization of oxygen remaining in the influent. The denitrification efficiency and specific denitrification rate were 47% and 1.69 mg $NO_3{^-}-N/g\;VSS{\cdot}hr$, respectively, which were slightly lower than for methanotrophic denitrification under an aerobic condition. The average concentration of total organic carbon in the effluent was as low as 2.45 mg/L, which indicates that it can be applicable as a post-denitrification method for the reclamation of secondary wastewater effluent. The dominant fatty acid methyl ester of mixed culture in the bioreactor was $C_{16:1{\omega}7c}$ and $C_{18:1{\omega}7c}$, which was predominantly found in type I and II methanotrophs, respectively. This study presents the potential of methanotrophic denitrification without externally excess oxygen supply as a post-denitrification option for various water treatment or reclamation.

• Biotechnology and Bioprocess Engineering:BBE
• /
• v.7 no.6
• /
• pp.352-356
• /
• 2002

Aeration was found to affect the biological denitrification by Ochrobactrum authropi SY509. Although cell growth was vigorous under 1 vvm of aeration and an agitation speed of 400 rpm in a 3-L jar fermentor, almost no nitrate was removed. Yet under low agitation speeds (100, 200, and 300 rpm), denitrification occurred when the dissolved oxygen was exhausted shortly af-ter the inoculation of the microorganism. Ochrobactrum authropi SY509 was found to express highly active denitrifying enzymes under anaerobic conditions. The microorganism also synthesized denitrifying enzymes under aerobic conditions (1 vvm and 400 rpm), yet their activity was only 60% of the maximum level under anaerobic conditions and the nitrate removal efficiency was merely 15%. However, although the activities of the denitrifying enzymes were inhibited in the presence of oxygen, they were fully recovered when the conditions were switched to anaerobic conditions.

• Journal of Korean Society on Water Environment
• /
• v.20 no.5
• /
• pp.480-487
• /
• 2004

This study was conducted to find the effect of electron acceptors on the formation of granular sludge by using four different types of electron acceptors. The phosphorous uptake, denitrification, and sulfate reduction in anoxic modes were simultaneously occured because of the presence of the polyphosphate accumultating organism(PAO) that utilize nitrate and sulfate as an electron acceptor in the anoxic zone. Denitrirying phosphorous removal bacteria(DPB) was enriched under anaerobic/anoxic/aerobic condition with a nitrate as an electron acceptor, and desulfating phosphorous removal bacteria(DSPB) was enriched under anaerobic/anoxic/aerobic condition with a sulfate as an electron acceptor. Polyphosphate accumulating organism(PAO) were enriched in the anaerobic/aerobic SBR. PAO took up acetate faster than DPB and DSPB during the aerobic phase. The sludge with nitrate and sulfate as an electron acceptors grew as a granules which possessed high activity and good settleability. In the anaerobic/aerobic modes, typical floccular growth was observed. In the result of bench-scale experiment, simultaneous reactions of phosphorus uptake, denitrification and sulfate reduction were observed under anoxic condition with nitrate and sulfate as an electron acceptors. These results demonstrated that the anaerobic/anoxic modes with nitrate and sulfate as an electron acceptors played an important role in the formation of the sludge granulation.

• Journal of Life Science
• /
• v.9 no.2
• /
• pp.40-43
• /
• 1999

In the wastewater treatment experiment by anaerobic-aerobic packed bed unit, it was found that the high and stable removal efficiency of nitrogen could be obtained. The extent of nitrogen removal gradually decreased with the rise of recycle ratio and DO concentration. On the other hand, the extent of phosphorus increased with the increase of DO concentration. COD showed high removal efficiency over the entire range tested. The simulation of T-N behavior was carried out satisfactorily by using the kinetic equations for biofilm and the reactor model which considered the packed bed as a plug flow reactor.

• Journal of Microbiology and Biotechnology
• /
• v.14 no.3
• /
• pp.639-642
• /
• 2004

The pH as a control parameter for oxidation-reduction potential (ORP) was investigated through the denitrification by Ochrobactrum anthropi SY509 under non-growing condition. The optimal pH of nitrate reductase was 7.0, and the minimal ORP level was －250 mV for the denitrification under aerobic condition. In the case of anaerobic condition, the optimal pHs of nitrate and nitrite reductase were shifted to 10.0 and 9.0, respectively, and the minimal ORP levels of nitrate and nitrite reductase were decreased to －370 mV and －340mV, respectively. In the case of alkaline pH and anaerobic condition, the denitrification efficiency of nitrate was increased up to about 2-fold over that of neutral pH and anaerobic condition. Therefore, the combined control of pH and ORP in the anaerobic condition is shown to be an important parameter in the biological denitrification process.